scholarly journals Extraction of Carnosic Acid and Carnosol from Sage (Salvia officinalis L.) Leaves by Supercritical Fluid Extraction and Their Antioxidant and Antibacterial Activity

Plants ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 16 ◽  
Author(s):  
Valentina Pavić ◽  
Martina Jakovljević ◽  
Maja Molnar ◽  
Stela Jokić
Keyword(s):  
Supercritical Fluid ◽  
Flow Rate ◽  
Acid Content ◽  
Antioxidant Activities ◽  
Salvia Officinalis ◽  
Carnosic Acid ◽  
Antioxidant Compounds ◽  
Bacterial Strains ◽  
Salvia Officinalis L ◽  
Co2 Flow

Sage (Salvia officinalis L.) is a good source of antioxidant compounds, carnosic acid and carnosol being the prominent ones. Both are soluble in CO2, and our goal was to investigate the application of supercritical CO2 extraction to obtain sage extracts rich in these compounds. The effect of pressure, temperature, and CO2 flow rate on the carnosic acid and carnosol yield was studied. These variables were optimized by response surface methodology (RSM). The pressure significantly affected carnosol extraction, while the extraction of carnosic acid was affected by the pressure, temperature, and CO2 flow rate. Carnosic acid content varied from 0.29–120.0 µg mg−1, and carnosol content from 0.46–65.5 µg mg−1. The optimal conditions according to RSM were a pressure of 29.5 MPa, a temperature of 49.1°C, and a CO2 flow rate of 3 kg h−1, and the sage extract yield was calculated to be 6.54%, carnosic acid content 105 µg mg−1, and carnosol content 56.3 µg mg−1. The antioxidant activities of the sage extracts were evaluated by the scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH). Sage extract obtained at 30 MPa and 40°C with 2 kg h−1 CO2 flow rate with a carnosic acid content of 72 µg mg−1 and carnosol content of 55 µg mg−1 exhibited the highest antioxidant activity (80.0 ± 0.68%) amongst the investigated supercritical fluid extracts at 25 µg mL−1 concentration. The antimicrobial properties of extracts were tested on four bacterial strains: Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus. The extract with a carnosic acid content of 116 µg mg−1 and a carnosol content of 60.6 µg mg−1 was found to be the most potent agent against B. subtilis.

Optimization of Supercritical Fluid Extraction of Antioxidant Compounds from Venezuelan Rosemary Leaves

2012 ◽  
Vol 8 (4) ◽  
Author(s):  
Gabriela Caldera ◽  
Yalitza Figueroa ◽  
Maritza Vargas ◽  
Diego T. Santos ◽  
Germania Marquina-Chidsey
Keyword(s):  
Antioxidant Activity ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Antioxidant Activities ◽  
Extraction Time ◽  
Carnosic Acid ◽  
Antioxidant Compounds ◽  
Extraction Temperature ◽  
Fluid Extraction ◽  
Static Extraction

Abstract The extraction of the antioxidant compounds from rosemary can be achieved by means of various processes, such as hydrodistillation, soxhlet extraction, microwave-accelerated hydrodistillation (MAHD) and supercritical fluid extraction (SFE). The aim of our study was to optimize SFE variables such as extraction pressure, extraction temperature and static extraction time for the maximum extraction of carnosol and carnosic acid from Venezuelan rosemary (Rosmarinus officinalis L.) leaves by using 23 full factorial and Box-Behnken designs and Response Surface Methodology (RSM). The chemical characterization of the supercritical fluid extracts was achieved by high-performance liquid chromatography (HPLC), and the antioxidant activity was determined by the peroxide index assay. For the extraction of carnosol, the variables of extraction temperature and static extraction time were statistically significant, and for the extraction of carnosic acid, only the extraction temperature-static extraction time interaction was significant. At a fixed pressure, the highest extraction of carnosol and carnosic acid were achieved when the operational extraction conditions of extraction temperature and static extraction time were set at 48°C for 69 min and 64°C for 57 min, respectively. SFE extracts presented higher antioxidant activities than commercial and soxhlet extracts, and pure dibutyl hidroxytoluene (BHT). Since the carnosic acid/carnosol ratios presented in our SFE extracts are higher than those from other countries, possibly it would be expected that higher antioxidant activity values would be obtained for the Venezuelan extracts.

Drought effects on polyphenol composition and antioxidant activities in aerial parts of Salvia officinalis L.

2010 ◽  
Vol 33 (4) ◽  
pp. 1103-1111 ◽  
Author(s):  
Iness Bettaieb ◽  
Ibtissem Hamrouni-Sellami ◽  
Soumaya Bourgou ◽  
Ferid Limam ◽  
Brahim Marzouk
Keyword(s):  
Antioxidant Activities ◽  
Salvia Officinalis ◽  
Aerial Parts ◽  
Salvia Officinalis L ◽  
Drought Effects

Influence of sodium salicylate on rosmarinic acid, carnosol and carnosic acid accumulation by Salvia officinalis L. shoots grown in vitro

2015 ◽  
Vol 37 (8) ◽  
pp. 1693-1701 ◽  
Author(s):  
Margareta Kračun-Kolarević ◽  
Slavica Dmitrović ◽  
Biljana Filipović ◽  
Marija Perić ◽  
Danijela Mišić ◽  
...  
Keyword(s):  
Rosmarinic Acid ◽  
Sodium Salicylate ◽  
Salvia Officinalis ◽  
Carnosic Acid ◽  
Acid Accumulation ◽  
Salvia Officinalis L

scholarly journals The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.

2011 ◽  
Vol 75 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Izabela Grzegorczyk ◽  
Ireneusz Bilichowski ◽  
Elżbieta Mikiciuk-Olasik ◽  
Halina Wysokińska
Keyword(s):  
Rosmarinic Acid ◽  
Hplc Analysis ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Carnosic Acid ◽  
Antioxidant Compounds ◽  
Shoot Cultures ◽  
Methanolic Extracts ◽  
Salvia Officinalis L

This report describes the effect of triacontanol on shoot multiplication and production of antioxidant compounds (carnosic acid, carnosol and rosmarinic acid) in <em>S. officinalis</em> cultures grown on MS basal medium (agar solidified medium supplemented with 0.1 mg l<sup>-1</sup> IAA, 0.45 mg l<sup>-1</sup> BAP). It was found that shoot proliferation significantly increased when triacontanol at concentrations of 5, 10 or 20 µg l<sup>-1</sup> was added to the medium. HPLC analysis of acetone and methanolic extracts of sage shoots showed that the production of diterpenoids, carnosic acid/carnosol ratio, as well as, contents of rosmarinic acid were also affected by the treatment with triacontanol. The highest stimulation effect of triacontanol was observed on the production of carnosol, where the treatment with 20 µg l l<sup>-1</sup> increased the content of this diterpenoid 4.5-fold compared to that in the control (sage shoots growing on MS basal medium, only).

scholarly journals Supercritical Antisolvent Fractionation of Antioxidant Compounds from Salvia officinalis

2021 ◽  
Vol 22 (17) ◽  
pp. 9351
Author(s):  
Raquel Mur ◽  
Juan I. Pardo ◽  
M. Rosa Pino-Otín ◽  
José S. Urieta ◽  
Ana M. Mainar
Keyword(s):  
Antioxidant Activity ◽  
Rosmarinic Acid ◽  
Supercritical Co2 ◽  
Qsar Model ◽  
Salvia Officinalis ◽  
Training Dataset ◽  
Antioxidant Compounds ◽  
Supercritical Antisolvent ◽  
Pure Compounds ◽  
Co2 Flow

The increasing interest towards greener antioxidants obtained via natural sources and more sustainable processes encourages the development of new theoretical and experimental methods in the field of those compounds. Two advanced separation methods using supercritical CO2 are applied to obtain valuable antioxidants from Salvia officinalis, and a first approximation to a QSAR model relating molecular structure with antioxidant activity is explored in order to be used, in the future, as a guide for the preselection of compounds of interest in these processes. Separation experiments through antisolvent fractionation with supercritical CO2 were designed using a Response Surface Methodology to study the effect of pressure and CO2 flow rate on both mass yields and capability to obtain fractions enriched in three antioxidant compounds: chlorogenic acid, caffeic acid and rosmarinic acid which were tracked using HPLC PDA. Rosmarinic acid was completely retained in the precipitation vessel while chlorogenic and caffeic acids, though distributed between the two separated fractions, had a major presence in the precipitation vessel too. The conditions predicted for an optimal overall yield and enrichment were 148 bar and 10 g/min. Although a training dataset including much more compounds than those now considered can be recommended, descriptors calculated from the σ-profiles provided by COSMO-RS model seem to be adequate for estimating the antioxidant activity of pure compounds through QSAR.

Development of a Method of Analysis for 46 Pesticides in Fruits and Vegetables by Supercritical Fluid Extraction and Gas Chromatography/Ion TVap Mass Spectrometry

1995 ◽  
Vol 78 (3) ◽  
pp. 821-830 ◽  
Author(s):  
Steven J Lehotay ◽  
Konstantin I Eller
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Supercritical Fluid ◽  
Flow Rate ◽  
Fruits And Vegetables ◽  
Method Development ◽  
Ion Trap ◽  
Solid Phase ◽  
Ion Trap Mass Spectrometry ◽  
Co2 Flow

Abstract A multiresidue method using supercritical fluid ex-traction (SFE) and gas chromatography/ion trap mass spectrometry (GC/1TMS) was developed for analysis of 46 pesticides in fruits and vegetables. The SFE procedure used 2 commercial instruments that trapped the extracts on solid-phase material. Silica gel chemically bound to octadecylsi-lane (ODS) collected the extracted pesticides efficiently, and elution of the trap with acetonitrile gave high recoveries. Extracts thus obtained were sufficiently clean for subsequent GC/ITMS analysis. The SFE conditions were 320 atm and 60°C (0.85 g/mL CO2 density) and 1.6 mL/min CO2 flow rate for 6 extraction vessel volumes. Trapping on 1 mL ODS occurred at 10°C, and a 0.4 mL/min flow rate of acetonitrile at 40°–50°C was used to elute the pesticides. Quantitative and qualitative analyses of the 46 pesticides were performed simultaneously by GC/ITMS. Studies of fortified samples gave &gt;80% recoveries for 39 pesticides, and recoveries of &gt;50% for the other pesticides, except methamidophos and omethoate. Grapes, carrots, potatoes, and broccoli were used as samples during method development, and a blind experiment involving incurred and fortified samples was used to test the approach. Results of the blind study compared satisfactorily with results from 7 laboratories using traditional GC detectors and solvent-based extractions.

Phenolic antioxidant compounds produced by in vitro shoots of sage (Salvia officinalis L.)

Plant Science ◽  
2002 ◽  
Vol 162 (6) ◽  
pp. 981-987 ◽  
Author(s):  
Paula C Santos-Gomes ◽  
Rosa M Seabra ◽  
Paula B Andrade ◽  
Manuel Fernandes-Ferreira
Keyword(s):  
Salvia Officinalis ◽  
Phenolic Antioxidant ◽  
Antioxidant Compounds ◽  
In Vitro Shoots ◽  
Salvia Officinalis L
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