scholarly journals Isoenzymatic variability in progeny of Pinus mugo Turra x Pinus sylvestris L. hybrids from Bór na Czerwonem, in experimental culture

2014 ◽  
Vol 69 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Maria A. Bobowicz ◽  
Władysław Danielewicz
Keyword(s):  
Genetic Structure ◽  
Pinus Sylvestris ◽  
Genetic Similarity ◽  
Hybrid Population ◽  
Fixation Index ◽  
Pinus Mugo ◽  
Similarity Coefficients ◽  
Hybrid Swarm ◽  
Pure Species ◽  
Natural Hybrids

The article describes the genetic structure of 12-year old seedlings. They were grown in an experimental culture representing the progeny of natural hybrids of <em>Pinus mugo</em> Turra and <em>Pinus sylvestris</em> L. from the peatbog Bór na Czerwonem. The studies were carried out on seedlings, employing electrophoresis of enzymatic proteins from winter buds. Parallel isoenzymatic analyses were also conducted for trees of <em>P. mugo</em> Turra from Tatra Mountains and for <em>P. sylvestris</em> L. trees from Białowieża in order to determine similarities of the hybrids to the 'pure' species, thought to represent parental forms, and for natural hybrids from Bór na Czerwonem. The obtained electrophoretic patterns made the determination of genetic structure of the population possible, namely of the observed heterozygosity, expected heterozygosity, Wright's fixation index and coefficients of similarity according to Nei and Hedrick. Genetic distances between the populations were also estimated. The variability of 11 enzymatic loci demonstrated the highest level of variability in the population of hybrids. Coefficients of genetic differentiation (GST) and of gene flow, (Nm) suggested a close relationship of the hybrids to <em>P. mugo</em> which may indicate an introgressive type of the hybrid swarm, from which the studied trees originated. The analysis of genetic similarity coefficients, according to Nei and Hedrick, demonstrated also an extensive similarity of the two hybrid groups to <em>P. mugo</em>. However, upon analysis of individual enzymatic loci, some of them highly resembled <em>P. mugo</em> while others were similar to <em>P. sylvestris</em>. The closest genetic similarity was demonstrated for the group of hybrids from the peatbog and the respective of hybrides group from culture. The variable gene expression as well as asymmetric inheritance of isoenzymes provided additional evidence of the introgressive character of the studied trees from the hybrid population.

Genetic status of the putative hybrid swarms of mountain dwarf pine and Scots pine in contact zones of their distribution in Slovakia

Biologia ◽  
2015 ◽  
Vol 70 (10) ◽  
Author(s):  
Peter Maňka ◽  
Andrej Kormuťák ◽  
Martin Galgóci ◽  
Dušan Gömöry
Keyword(s):  
Genetic Structure ◽  
Scots Pine ◽  
Molecular Level ◽  
Natural Populations ◽  
Hybrid Origin ◽  
Putative Hybrid ◽  
Hybrid Swarm ◽  
Pure Species ◽  
Hybrid Swarms ◽  
History Of

AbstractGenetic structure of the mountain dwarf pine (Pinus mugo) and Scots pine (P. sylvestris) natural populations along with their 4 putative hybrid swarms in Slovakia were analyzed using 12 enzyme loci. The study aimed in evaluation of postulated hybrid status of the swarms on molecular level. Based on semidiagnostic allele frequencies of the MDH B, MDH C, 6PGDH B, ADH 1 and F-EST loci in their megagametophytes and vegetative buds, the conspicuous genetic differences were detected between P. mugo and P. sylvestris populations. The putative hybrid swarms seem to incline by their genetic structure to P. mugo rather than to P. sylvestris. Obtained results indicate independent developmental history of each of the scored hybrid swarms. The putative hybrid swarm in Sucha Hora was found to be a mixed stand consisting prevailingly of pure-species individuals of P. mugo and P. sylvestris. On the contrary, the putative hybrid swarms in Habovka, Tisovnica and Terchova are supposed to be of hybrid origin.

scholarly journals Biometrical study of some individuals chosen from Pinus mugo turra populations in the peat bog "Bór na Czerwonem"

2014 ◽  
Vol 64 (1) ◽  
pp. 71-80 ◽  
Author(s):  
Alina Bączkiewicz
Keyword(s):  
Pinus Sylvestris ◽  
Parental Species ◽  
Growth Trait ◽  
Morphological Characters ◽  
Peat Bog ◽  
Pinus Mugo ◽  
Hybrid Swarm ◽  
Multivariate Statistical ◽  
One Year ◽  
Biometrical Study

Sixteen individuals were sampled for study of variation in 17 anatomical and morphological characters. Only individuals of low polycormic growth (trait typical for <i>Pinus mugo</i> Turra) connected with incurved one-year-cone stipes (a similar situation exists in <i>Pinus sylvestris</i> L.) were chosen, thus the sample studied cannot be treated as a random one. It has been shown by multivariate statistical analysis that these 16 individuals are quite different from each other, Mahalanobis'generalized distances between them being nearly 50% significantly different from 0. The sample studied in this respect is distinctly different from pure stands of both putative parental species (i.e. <i>Pinus mugo</i> and <i>P. sylvestris</i>). Every plant studied shows a different combination of traits typical (or nearly typical) for both the above-mentioned species and traits that are truly intermediate between them. The results support the frequently expressed opinion that the mountain pine population from the peat bog "Bór na Czerwonem" is, in fact, a hybrid swarm formed by hybridization between <i>Pinus mugo</i> and <i>Pinus sylvestris</i>.

scholarly journals The genetic structure of admixed populations.

Genetics ◽  
1991 ◽  
Vol 127 (2) ◽  
pp. 417-428 ◽  
Author(s):  
J C Long
Keyword(s):  
Natural Selection ◽  
Genetic Structure ◽  
Genetic Drift ◽  
Hybrid Population ◽  
Fixation Index ◽  
Human Populations ◽  
Random Errors ◽  
Chi Square ◽  
Testing Procedures ◽  
Chi Square Test

Abstract A method for simultaneously estimating the admixture proportions of a hybrid population and Wright's fixation index, FST, for that hybrid is presented. It is shown that the variance of admixture estimates can be partitioned into two components: (1) due to sample size, and (2) due to evolutionary variance (i.e., genetic drift). A chi-square test used to detect heterogeneity of admixture estimates from different alleles, or loci, can now be corrected for both sources of random errors. Hence, its value for the detection of natural selection from heterogeneous admixture estimates is improved. The estimation and testing procedures described above are independent of the dynamics of the admixture process. However, when the admixture dynamics can be specified, FST can be predicted from genetic principles. Two admixture models are considered here, gene flow and intermixture. These models are of value because they lead to very different predictions regarding the accumulation of genes from the parental populations and the accumulation of variance due to genetic drift. When there is not evidence for natural selection, and it is appropriate to apply these models to data, the variance effective size (Ne) of the hybrid population can be estimated. Applications are made to three human populations: two of these are Afro-American populations and one is a Yanomamö Indian village. Natural selection could not be detected using the chi-square test in any of these populations. However, estimates of effective population sizes do lead to a richer description of the genetic structure of these populations.

scholarly journals Changes of genetic structure of Pinus sylvestris L. populations exposed to industrial pollution

2014 ◽  
Vol 66 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Aleksandra Wojnicka-Półtorak
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Pinus Sylvestris ◽  
Industrial Pollution ◽  
Fixation Index ◽  
Control Group ◽  
Provenance Trial ◽  
Phosphorus Fertilizers ◽  
The One ◽  
Nitrogen Phosphorus

Isoenzymatic variation of <em>Pinus sylvestris</em> was analysed in populations from a provenance trial (IUFRO 1982) in Kórnik and their replication in Luboń. The two groups of populations originated from the same seed pool, but the one in Luboń was exposed to pollutants emitted during the production of nitrogen-phosphorus fertilizers, while the Kórnik provenance trial were used as a control group. The parameters determining the genetic structure of the studied populations - frequency of alleles and genotypes (average number of alleles and genotypes per locus), observed heterozygosity (Ho), and genotypic polymorphism index (Pg) - have higher values in the group of trees tolerant to pollution. Also fixation index (F), which indicates that homozygotic genotypes underwent selection in this group, as well as dendrites and dendrograms according to Nei and Hedrick, suggest that the genetic structure of the populations exposed to pollution was changed. The obtained results indicate that populations characterized by a greater genetic diversity are more tolerant to pollution.

scholarly journals Serological characteristics of some putative hybrid individuals from a Pinus sylvestris x Pinus mugo hybrid swarm population

2014 ◽  
Vol 49 (1-2) ◽  
pp. 127-142 ◽  
Author(s):  
Wiesław Prus-Gowacki ◽  
Jerzy Szweykowski
Keyword(s):  
Pinus Sylvestris ◽  
The Other ◽  
Putative Hybrid ◽  
Pinus Mugo ◽  
Hybrid Swarm ◽  
Common Trend ◽  
Antigenic Properties ◽  
Enzymatic Systems

The antigenic properties of some putative hybrid individuals from a hybrid swarm population were studied. All the plants were to a major or lesser degree intermediate between three pure taxa, i.e. <em>P. sylvestris, P. mugo</em> and <em>P. uliginosa</em>. The plants are, however, not intermediate in general, but are sin-Mar to one species in part of their antigenic properties (enzymatic systems) and to the other one in the rest. It means that there is no common trend in the variation of the antigenic characters (proteins) and they behave independently.

CONNECTION g.307 G > A SNP POLYMORPHISM OF THE ALPHA-FUKOZYLTRASFERAZA 1 GENE WITH ECONOMIC-USEFUL TRAITS IN THE LARGE WHITE PIG BREED

2017 ◽  
Vol 54 ◽  
pp. 134-140
Author(s):  
H. S. Rudoman ◽  
V. M. Balatsky ◽  
V. Y. Nor ◽  
V. O. Vovk
Keyword(s):  
Genetic Structure ◽  
Information Content ◽  
Analysis Of Variance ◽  
Farm Animals ◽  
Daily Weight Gain ◽  
Fixation Index ◽  
Its Sequencing ◽  
Large White ◽  
Stage Of Development ◽  
High Level

One of the top priorities at the present stage of development of pig breeding remains the development of a set of measures aimed at increasing the resistance animals to various diseases, especially – to colibacteriosis .One of the recent and effective approaches to prevent colibacillosis is using markers of selection; it involves pig genotyping by genome locuses. Chosen locuses are associated with animal sensitivity to the disease and selection of the results of genotyping of animals with increased resistance. Due to researches, one of such locuses is alpha-fukozyltrasferаza 1 gene (FUT1). Gene FUT1 is located in chromosome 6. As a result of its sequencing in the swine breeds of Large White and Swedish Landrace, single-nucleotide polymorphism (g.307 G > A SNP) has been detected. AA genotype determines the resistance of animals to colibacteriosis, while AG and GG genotypes are susceptible to this disease. According to the results of previous studies, the positive effect of allele A was determined not only on the resistance of pigs to colibacteriosis, but also on indicators of fattening and meat productivity and on reproductive performance. In Ukraine, the studies of polymorphism FUT1 g.307 G > A SNP were held fragmentedly and only on certain populations of Ukrainian Meat and Large White breed but without establishing its association with the indicators of productivity of pigs. The aim of our work was to study the genetic structure of Ukrainian Large White breeds, type 1 and the establishment of association. g. 307 G > A SNP FUT1 gene with pigs indicators of productivity. For research the 96 samples of hair were used. DNA isolation from samples with biomaterial were carried out using ion exchange resin Chelex-100. Genotyping was performed by PCR-RFLP by method of Jorgensen et al. (2006). Using DNA analysis of this breeds locus FUT1 was determined by genetic structure. Allele frequency of allele G (0,573) and allele A (0,427) was established The distribution of genotype frequencies was not statistically significantly different from the theoretically expected, calculated by the Hardy-Weinberg criterion. Thus, according to the locus of FUT1 g.307 G > A, the breeds investigated are in a state close to the genetic equilibrium. The negative value of the fixation index by the locus FUT1 g.307 G > A indicates an excess of heterozygotes in the population, and hence the lack of targeted selection for this marker. The valuation was performed by calculating the PIC (polymorphic information content) – information content of polymorphism marker. In the analyzed pig herd for this marker, the PIC has an average value (0,367), which indicates the high level of polymorphism of the given locus and is favorable for the possibility of conducting a search for links between individual genotypes and indicators of productivity. To establish the association g.307 G > A SNP FUT1 gene with indicators of animal productivity, a one-way analysis of variance of the experimental data was used. Based on the results of a one-way analysis of variance, a significant effect of the genotypes of the FUT1gene (g.1849 G > C) on the indicator of the average daily weight gain (р ≤ 0,001), the thickness of the bacon at the level of the VI-VII vertebrae (p ≤ 0.01), reaching live weight of 100 kg (р ≤ 0,001) and breeding index of fattening qualities (р ≤ 0,01) was found. The parameter of the influence of the genetic factor on the test feature was 28,54%, 10,4%, 33,4% and 8,8% respectively. According to the investigated indicators of productivity, animals with genotype AA, which determines resistance to colibacteriosis, dominated the animals with genotypes GG and AG. Based on the results of our research and pre-published data, the multiple effect of the g.307 G > A SNP FUT1 gene is observed, which is associated with the indicators of productivity, which again confirms the polygenicity of the quantitative trait loci of farm animals. Taking into account a high level of polymorphism of the investigated gene and reliable associations of genotypes with indicators of productivity found, it can be recommended to carry out breeding of pigs using genetic information of the g.307 G > A SNP gene FUT1.

Phylogenetic relationships of five morphological groups of hexaploid wheat (Triticum aestivum L. em Thell.) based on RAPD analysis

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 724-727 ◽  
Author(s):  
Wenguang Cao ◽  
G Scoles ◽  
P Hucl ◽  
R N Chibbar
Keyword(s):  
Hexaploid Wheat ◽  
Phylogenetic Relationships ◽  
Genetic Similarity ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Triticum Aestivum L ◽  
Similarity Coefficients ◽  
Morphological Classification ◽  
Wild Wheat

The genetic relationships among the five groups of hexaploid wheat: common, spelta, macha, vavilovii, and semi-wild wheat (SWW) are not clear. Random amplified polymorphic DNA (RAPD) analysis was used to assess phylogenetic relationships among these five morphological groups of hexaploid wheat. RAPD data were analyzed using the NTSYS-PC computer program to generate Jaccard genetic similarity coefficients. A dendrogram based on RAPD analysis grouped 15 accessions into five distinct clusters. These results are in agreement with those based on morphological classification, suggesting that common wheat is most closely related to SWW, followed by spelta, vavilovii, and macha.Key words: RAPD, macha, spelta, vavilovii, semi-wild wheat, phylogenetic relationships.

Population Dynamics and Regeneration of a Hybrid Zone Between Eucalyptus risdonii Hook-F and E amygdalina Labill

1986 ◽  
Vol 34 (3) ◽  
pp. 305 ◽  
Author(s):  
BM Potts
Keyword(s):  
Hybrid Zone ◽  
Null Point ◽  
Asymmetric Distribution ◽  
Hybrid Swarm ◽  
Population Response ◽  
Pure Species ◽  
Seed Migration ◽  
Hybrid Swarms ◽  
Population Turnover ◽  
Phenotypic Fitness

Regeneration of a hybrid zone between E. amygdalina and E. risdonii and pure species stands following wildfire is reported, as well as the reproductive and vegetative fitness of parental and hybrid phenotypes. E. risdonii phenotypes dominated the seed rain and seedling cohort and there was clearly a marked fitness differential between E. amygdalina and E. risdonii at their boundary. When the F1 type hybrid is in competition with both parental types it is generally reproductively the least fit, although frequently vegetatively vigorous. Reduced fitness appears to extend to advanced generations as hybrid phenotypes tending.toward either species are, on average, less fit than the corresponding parental type. The pattern of phenotypic fitness suggests that the species' boundary is in disequilibrium and it is argued that E. risdonii is invading the range of E. amygdalina by both pollen and seed migration. There is an asymmetric distribution of F1 type hybrids across the boundary and the hybrid swarm examined is being invaded by E. risdonii genes. It is suggested that hybridization may be associated with natural disequilibrium and, where seed migration is limited, boundary movements may be preceded by a wave of hybridization due partly to pollen swamping of the least fit species. Hybrid swarms may develop but, at the boundary of large stands, are probably transitory. There is a marked inertia in the population response to the prevailing selective regime due to the extremely slow population turnover and limited dispersal potential. This is discussed in the broader context of non-equilibrium models where it is argued that dispersal may be the factor limiting population response to perturbation of a shallow environmental gradient. This is due to large geographical shifts in the position of the null point and would be accentuated in a patchy environment where migration as a front is prevented.

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