scholarly journals Changes of genetic structure of Pinus sylvestris L. populations exposed to industrial pollution

2014 ◽  
Vol 66 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Aleksandra Wojnicka-Półtorak
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Pinus Sylvestris ◽  
Industrial Pollution ◽  
Fixation Index ◽  
Control Group ◽  
Provenance Trial ◽  
Phosphorus Fertilizers ◽  
The One ◽  
Nitrogen Phosphorus

Isoenzymatic variation of <em>Pinus sylvestris</em> was analysed in populations from a provenance trial (IUFRO 1982) in Kórnik and their replication in Luboń. The two groups of populations originated from the same seed pool, but the one in Luboń was exposed to pollutants emitted during the production of nitrogen-phosphorus fertilizers, while the Kórnik provenance trial were used as a control group. The parameters determining the genetic structure of the studied populations - frequency of alleles and genotypes (average number of alleles and genotypes per locus), observed heterozygosity (Ho), and genotypic polymorphism index (Pg) - have higher values in the group of trees tolerant to pollution. Also fixation index (F), which indicates that homozygotic genotypes underwent selection in this group, as well as dendrites and dendrograms according to Nei and Hedrick, suggest that the genetic structure of the populations exposed to pollution was changed. The obtained results indicate that populations characterized by a greater genetic diversity are more tolerant to pollution.

scholarly journals Genetic diversity and spatial genetic structure in populations of Orbignya phalerata Mart. under different exploitation intensities in the Brazilian savanna

2015 ◽  
Vol 64 (1-6) ◽  
pp. 201-211 ◽  
Author(s):  
Bruna Ibanes ◽  
A.M. Sebbenn ◽  
V.C.R. Azevedo ◽  
M.A. Moreno ◽  
F.B. Gandara ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Spatial Genetic Structure ◽  
Spatial Distance ◽  
Null Alleles ◽  
Fixation Index ◽  
Genetic Studies ◽  
Expected Heterozygosity ◽  
Adult Trees ◽  
Diversity Structure

Abstract Genetic studies in tropical tree species have found signs of decreased genetic diversity and increased levels of inbreeding and spatial genetic structure (SGS) in fragmented and exploited populations. The aim of this paper was to investigate genetic diversity, structure, and intrapopulation SGS using eight microsatellite loci for three Orbignya phalerata populations that have undergone different intensities of seed harvesting. From each population, we georeferenced and sampled 30 seedlings, 30 juveniles, and 30 adult trees. The total number of alleles over all loci (k), and observed (Ho) and expected heterozygosity (He) presented lower values for the population experiencing more intense fruit harvesting than less heavily exploited populations, suggesting that fruit harvesting may decrease genetic diversity. Null alleles were detected in practically all loci among seedlings, juveniles, and adults in all populations, indicating that the estimates of Ho, He, and fixation index (F) are biased. When corrected for null alleles (FNull), the fixation index decreased for all samples, resulting in significantly higher than zero results for seedlings of all populations, but not for juveniles and adults of all populations. The comparison of FNull values between cohorts in the most heavily exploited population (ESP) suggests that inbred individuals are eliminated between seedling and adult stages. Significant SGS was detected up to 60 m in all populations, which indicates short distance seed dispersal. Genetic differentiation (G’ST) between pairwise populations was related to spatial distance between populations, with the greatest difference between more distant populations.

scholarly journals Genetic diversity and differentiation of Pinus sylvestris L. from the IUFRO 1982 provenance trial revealed by AFLP analysis

2015 ◽  
Vol 67 (4) ◽  
pp. 1237-1249
Author(s):  
Piotr Androsiuk ◽  
Sylwia Ciaglo-Androsiuk ◽  
Lech Urbaniak
Keyword(s):  
Genetic Diversity ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Aflp Analysis ◽  
Provenance Trial ◽  
Migration Routes ◽  
Bulked Dna ◽  
Individual Character ◽  
The Individual ◽  
Diversity Studies

DNA markers have become effective tools in genetic diversity studies of forest trees. However, molecular marker analyses are associated with laborious and costly effort. One of the possibilities to overcome these constraints is to analyze bulked samples per population, rather than individual plants. We have used bulked DNA-based AFLP analysis to investigate genetic variations in Pinus sylvestris L. (Scots pine) from the IUFRO 1982 provenance trial in K?rnik (western Poland). Four AFLP primer combinations yielded a total of 309 bands, of which 208 (67.31%) were polymorphic. Thirty-six (11.65%) unique alleles were deployed randomly among the populations. Estimated genetic diversity and differentiation was high, as expressed by He = 0.238 and I = 0.356, and by genetic distance values which ranged from 0.154 to 0.363. A geographic pattern of interpopulation differentiation was observed, pointing to the individual character of populations from northeastern Europe. In the light of available data, we discuss the influence of historical migration routes, gene flow and human activity on observed genetic diversity and differentiation of Scots pine in Europe. Our results indicate that the AFLP method applied to DNA templates extracted from bulked leaf samples provides an efficient approach to elucidate genetic diversity and relationships among Scots pine populations.

scholarly journals The effects of fragmentation on the genetic structure of Theobroma speciosum (Malvaceae) populations

2017 ◽  
Vol 66 (1) ◽  
pp. 218 ◽  
Author(s):  
Juliana De Freitas Encinas Dardengo ◽  
Ana Aparecida Bandini Rossi ◽  
Tatiane Lemes Varella
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
National Park ◽  
Urban Forest ◽  
Fixation Index ◽  
Forest Fragments ◽  
Mato Grosso ◽  
Increasing Trend ◽  
Fragmented Population ◽  
Forest Parks

Native Theobroma species, such as cacauhy, are losing their habitat due to the intense forest fragmentation in the Amazon region, and preserving their genetic diversity has been the main aim of many conservation programs. The aim of the present study is to assess whether fragmentation and habitat reduction affect the genetic structure and lead to genetic diversity losses in natural Theobroma speciosum populations. The study was conducted in two Mato Grosso State (Brazil) locations, namely: Apiacás and Alta Floresta counties. Juruena National Park (JNP) in Apiacás County holds a natural T. speciosum population that has not underwent anthropic influences. A population composed of individuals from three anthropized urban forest parks (UF) in Alta Floresta County was analyzed. The leaves of 75 T. speciosum individuals distributed in the urban forest fragments and of 100 individuals found in the Juruena National Park were sampled. All nine microsatellite loci showed high polymorphism levels between categories (adults and sub-adults), in both populations. The sub-adult individuals of the population (UF) in the fragmented area showed higher value (0.71), whereas the preserved population (JNP) category presented the same value (0.69). The increasing trend of estimating the fixation index towards the fragmented population was observed. The analysis of molecular variance showed 83% genetic diversity within categories; 16%, between populations; and only 1%, between categories. Although the effects were small, a persistent fragmentation process can increase the inbreeding levels and facilitate the genetic drift action. These effects may lead the T. speciosum populations to inbreeding depression, diversity loss and genetic structure change in the course of several generations.

scholarly journals Genetic diversity of Anadenanthera colubrina Vell. (Brenan) var cebil, a tree species from the South American subtropical forest as revealed by cpSSR markers

2011 ◽  
Vol 60 (1-6) ◽  
pp. 123-132 ◽  
Author(s):  
M. E. Barrandeguy ◽  
M.V. García ◽  
C. F. Argüelles ◽  
G. D. L. Cervigni
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Tree Species ◽  
Evolutionary Dynamics ◽  
Pairwise Comparison ◽  
Fixation Index ◽  
Haplotypic Diversity ◽  
Adult Trees ◽  
Minimum Spanning Network ◽  
Subdivided Populations

Abstract Anadenanthera colubrina var cebil is a tree species native to the Upper Parana Atlantic Forest where human activities have severely impacted causing deep fragmentation. Microsatellites are not available in this species. Therefore, the first objective of this study was to generate chloroplast simple sequence repeats (cpSSR) by cross-species transfer. Understanding the evolutionary dynamics of subdivided populations is an important matter. In this way, a first approach to the characterization of the haplotypic diversity within and between populations as well as the genetic structure of native Argentinean populations were the main goals of this study. Twenty four individuals from two populations of the Misiones province were studied and four cpSSR loci were tested. Two of them exhibited polymorphic patterns leading to the identification of 11 cpDNA haplotypes with high mean genetic diversity (GD=0.73). The minimum spanning network defined three clear groups which can be assigned to at least three subpopulations. AMOVA indicated that the total variance showed the highest percentage of variation (48%) within subpopulations with a fixation index (FST) statistically significant (FST=0.520; p<0.05). Brown’s two loci component analysis indicated that substructure population is present. Jost’s differentiation global index (Dest) was 0.049 while Dest pairwise comparison reflected a certain level of genetic structure. The high diversity level detected in the adult trees of A. colubrina var cebil from the populations under study could be due to recent human influence. In this way, the reduction in population size caused a reduction in the number of trees leading to surviving trees showing the historical diversity of the populations analyzed.

scholarly journals The genetic structure of Scots pine (Pinus sylvestris L.) population from the top of Szczeliniec Wielki Mt., Central Sudetes

2014 ◽  
Vol 63 (3-4) ◽  
pp. 315-324 ◽  
Author(s):  
Jerzy Szweykowski ◽  
Wiesław Prus-Głowacki ◽  
Jerzy Hrynkiewicz
Keyword(s):  
Genetic Structure ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Environmental Conditions ◽  
Genetic Parameters ◽  
Geographic Region ◽  
Common Origin ◽  
Provenance Trial ◽  
Great Similarity

The comparison of the genetic stucture of the population of Scots pine from Szczeliniec Wielki Mt. with the population of its progeny and three other populations from the same geographic region shows great similarity of both populations as far as the frequencies of genes and genotypes are concerned. Also other genetic parameters, such as heterozygosity, genotypic polymorphism indices and a number of alleles per locus were in both populations very similar. It seems that our data proved that the provenance trial experiments reflect rather closely the genetic structure of maternal populations. The fact that the population from Szczeliniec Wielki and its "replica" are genetically most similar to the geographically closest population from Bystrzyca Kłodzka leads to the conclusion, that these populations are of common origin. Though the relic character of the population from Szczeliniec can not be excluded. The morphological distinctness of the population from Szczeliniec is caused by a modifying influence of environmental conditions.

PSXII-8 Mitochondrial DNA diversity within domestic reindeer in Russia

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 253-253
Author(s):  
Veronika R Kharzinova ◽  
Arsen V Dotsev ◽  
Nikolay V Bardukov ◽  
Tatiana E Deniskova ◽  
Maulik Upadhyay ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
R Package ◽  
Cytb Sequence ◽  
Cultural Survival ◽  
Census Size ◽  
Mtdna Diversity ◽  
The One ◽  
Genetic Structure And Diversity ◽  
Sequences Alignment

Abstract Domestic reindeer in Russia are a valuable resource of vital importance to the physical and cultural survival of the Northern indigenous minority. During the last decades, the mitochondrial (mt) genetic markers have been widely used as a molecular tool to investigate genetic structure and diversity of livestock species. Here we aimed at the assessing the mtDNA diversity of the domestic reindeer inhabiting the area from the Kola Peninsula in the west to the Chukotka region in the east. A complete cytochrome b (cytb) sequences (1,140 bp) from representatives of six populations, including Nenets (NEN, n = 16), Evenk (EVK, n = 12), Even (EVN, n = 6), Chukotka (CHU, n = 6), Chukotka-Khargin (CHUKH, n = 6) and Tuva (TUVA, n = 6) were obtained. Sequences’ alignment was conducted using MUSCLE algorithm in R package msa. In total, 34 haplotypes were identified. Median-joining network, constructed in PopART 1.7, revealed three major groups of haplotypes: the first one joined the samples of all the populations, the second one included NEN, EVN and CHUKH, and the third group was presented by the one sample of CHU. AMOVA, calculated in Arlequin 3.5.2.2, showed that only 9.58% of molecular variance could be explained by the differences between populations and 90.42% - within populations. Genetic diversity parameters calculated in DnaSP 6.12.03, demonstrated that average number of nucleotide differences (K) was highest in CHUKH (28.333) and EVN (27.409) and lowest in TUVA (4.533) and EVK (5.400). Nucleotide diversity (Pi) was 0.01238±0.00559, 0.00474±0.00091, 0.02404±0.00453, 0.01281±0.00464, 0.02485±0.00744, and 0.00398±0.00110 for NEN, EVK, EVN, CHU, CHUKH and TUVA, respectively. Our study demonstrated the lack of clear genetic structure of the studied reindeer populations in relation to cytb sequence. The level of genetic diversity was associated with census size and was lowest in the smallest Tuva population. This study was supported by RSF-21-16-00071 and Russian Ministry of Science and Higher Education-0445-2019-0024.

Population genetic structure andWolbachiainfection in an endangered butterfly,Zizina emelina(Lepidoptera, Lycaenidae), in Japan

2014 ◽  
Vol 105 (2) ◽  
pp. 152-165 ◽  
Author(s):  
Y. Sakamoto ◽  
N. Hirai ◽  
T. Tanikawa ◽  
M. Yago ◽  
M. Ishii
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Population Genetic ◽  
Nuclear Dna ◽  
Geographic Distance ◽  
Fixation Index ◽  
Migratory Activity ◽  
Dna Analyses ◽  
Mitochondrial Haplotypes ◽  
Male Killer

AbstractZizina emelina(de l'Orza) is listed on Japan's Red Data List as an endangered species because of loss of its principal food plant and habitat. We compared parts of the mitochondrial and nuclear genes of this species to investigate the level of genetic differentiation among the 14 extant populations. We also examined infection of the butterfly with the bacteriumWolbachiato clarify the bacterium's effects on the host population's genetic structure. Mitochondrial and nuclear DNA analyses revealed that haplotype composition differed significantly among most of the populations, and the fixation indexFSTwas positively correlated with geographic distance. In addition, we found three strains ofWolbachia, one of which was a male killer; these strains were prevalent in several populations. There was linkage between some host mitochondrial haplotypes and the threeWolbachiastrains, although no significant differences were found in a comparison of host mitochondrial genetic diversity with nuclear genetic diversity inWolbachia-infected or -uninfected populations. These genetic analyses andWolbachiainfection findings show thatZ. emelinahas little migratory activity and that little gene flow occurs among the current populations.

scholarly journals Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Forests ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 999
Author(s):  
Yulia Vasilyeva ◽  
Nikita Chertov ◽  
Yulia Nechaeva ◽  
Yana Sboeva ◽  
Nina Pystogova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Spatial Genetic Structure ◽  
Natural Populations ◽  
Geographic Location ◽  
East European Plain ◽  
Gene Pools ◽  
East European

In order to carry out activities aimed at conservation and rational use of forest resources; it is necessary to study the main forest-forming plant species in detail. Scots pine (Pinus sylvestris L., Pinaceae) is mainly found in the boreal forests of Eurasia and is not so often encountered in the east of the East European Plain. The aim of the study was to study the genetic diversity, structure and differentiation of Scots pine populations in the east of the East European Plain. We studied ten populations of P. sylvestris using the Inter Simple Sequence Repeats (ISSR)-based DNA polymorphism detection method. Natural populations are demonstrated by relatively high rates of genetic diversity (He = 0.167; ne = 1.279; I = 0.253). At the same time, there is a tendency for a decrease in the genetic diversity of the studied populations of P. sylvestris from west to east. Analysis of the genetic structure shows that the studied populations are highly differentiated (GST = 0.439), the intrapopulation component accounts for about 56% of the genetic diversity. Using various algorithms for determining the spatial genetic structure, it is found that the studied populations form two groups of populations in accordance with geographic location. With the help of a genetic originality coefficient, populations with specific and typical gene pools are identified. They are recommended as sources of genetic diversity and reserves for the conservation of genetic resources of the species.

scholarly journals Isoenzymatic variability in progeny of Pinus mugo Turra x Pinus sylvestris L. hybrids from Bór na Czerwonem, in experimental culture

2014 ◽  
Vol 69 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Maria A. Bobowicz ◽  
Władysław Danielewicz
Keyword(s):  
Genetic Structure ◽  
Pinus Sylvestris ◽  
Genetic Similarity ◽  
Hybrid Population ◽  
Fixation Index ◽  
Pinus Mugo ◽  
Similarity Coefficients ◽  
Hybrid Swarm ◽  
Pure Species ◽  
Natural Hybrids

The article describes the genetic structure of 12-year old seedlings. They were grown in an experimental culture representing the progeny of natural hybrids of <em>Pinus mugo</em> Turra and <em>Pinus sylvestris</em> L. from the peatbog Bór na Czerwonem. The studies were carried out on seedlings, employing electrophoresis of enzymatic proteins from winter buds. Parallel isoenzymatic analyses were also conducted for trees of <em>P. mugo</em> Turra from Tatra Mountains and for <em>P. sylvestris</em> L. trees from Białowieża in order to determine similarities of the hybrids to the 'pure' species, thought to represent parental forms, and for natural hybrids from Bór na Czerwonem. The obtained electrophoretic patterns made the determination of genetic structure of the population possible, namely of the observed heterozygosity, expected heterozygosity, Wright's fixation index and coefficients of similarity according to Nei and Hedrick. Genetic distances between the populations were also estimated. The variability of 11 enzymatic loci demonstrated the highest level of variability in the population of hybrids. Coefficients of genetic differentiation (GST) and of gene flow, (Nm) suggested a close relationship of the hybrids to <em>P. mugo</em> which may indicate an introgressive type of the hybrid swarm, from which the studied trees originated. The analysis of genetic similarity coefficients, according to Nei and Hedrick, demonstrated also an extensive similarity of the two hybrid groups to <em>P. mugo</em>. However, upon analysis of individual enzymatic loci, some of them highly resembled <em>P. mugo</em> while others were similar to <em>P. sylvestris</em>. The closest genetic similarity was demonstrated for the group of hybrids from the peatbog and the respective of hybrides group from culture. The variable gene expression as well as asymmetric inheritance of isoenzymes provided additional evidence of the introgressive character of the studied trees from the hybrid population.

scholarly journals Molecular Genetic Analysis of Pinus sylvestris L. and Pinus sibirica Du Tour Populations in Perm Krai Based on Polymorphism ISSR-PCR markers

2021 ◽  
Vol 7 (4) ◽  
Author(s):  
Yu. Nechaeva ◽  
N. Pystogova ◽  
N. Chertov ◽  
S. Boronnikova
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Pinus Sylvestris ◽  
Gene Pools ◽  
Pinus Sibirica ◽  
Pcr Markers ◽  
High Genetic Diversity ◽  
Perm Krai ◽  
Pinus Sibirica Du Tour ◽  
Issr Pcr

DNA polymorphism has been studied, indicators of genetic diversity and genetic structure of 3 populations of Pinus sylvestris L. and 3 populations of Pinus sibirica Du Tour in the Perm Krai have been determined. In the populations of P. sibirica, 102 ISSR-PCR markers were found, of which 88 were polymorphic (P95 = 0.863), and in the populations of P. sylvestris — 113 ISSR-PCR markers, 100 of which were polymorphic (P95 = 0.885). The populations of the two studied species of woody plants are characterized by high genetic diversity. At the same time, in P. sibirica, the indices of genetic diversity were slightly higher (HE = 0.195; ne = 1.335; na = 1.330) than in P. sylvestris (HE = 0.166; ne = 1.268; na = 1.212). The analysis of the genetic structure showed that the coefficient of genetic subdivision (GST) in the two studied species of the genus Pinus are similar and amount to 0.320 in P. sibirica and 0.303 in P. sylvestris. The populations of Siberian pine and Scots pine are characterized by an average degree of genetic differentiation, since the interpopulation component accounts for 32.0% and 30.3% of the genetic diversity of these species, respectively. Using the Mantel test, a high correlation was found between genetic and geographical distances in P. sibirica populations (R2 = 0.6871), while P. sylvestris showed a low correlation (R2 = 0.0649). The data obtained are relevant for the preservation of the gene pools of the studied two species of the genus Pinus in the Perm Krai.

Sign in / Sign up

Export Citation Format

Share Document