scholarly journals Influence of hydroxyurea on nucleic acids content and 3H-uridine incorporation in callus and tumorous tobacco tissues cultured in vitro

2015 ◽  
Vol 44 (3) ◽  
pp. 393-405
Author(s):  
A. Bielecka
Keyword(s):  
Nicotiana Tabacum ◽  
Nucleic Acids ◽  
Dna Content ◽  
Incubation Medium ◽  
Rna Synthesis ◽  
Callus Tissue ◽  
Tumour Tissue ◽  
Inhibitory Influence ◽  
Tumor Tissues

In callus and tumor tissues of <i>Nicotiana tabacum</i> cultured for 39 days in media supplemented with various concentrations of hydroxyurea (1.3 x 10<sup>-4</sup> M - 1.3 x 10<sup>-3</sup> M) a decrease of DNA content (ca. 24 per cent in callus tissue and ca. 23 per cent in tumour tissue) and a decrease of RNA content (over 10 per cent and ca. 9 per cent in callus and tumour tissue, respectively) was observed. The autoradiographic method showed that a long-lasting action of this com-pound inhibits RNA synthesis. A stronger inhibitory influence of hydroxyurea upon incorporation of 3H-uridine from the incubation medium was revealed.

scholarly journals Fractionation of phosphorus compounds in tumor and callus tissues of Nicotiana tabacum

2015 ◽  
Vol 41 (4) ◽  
pp. 481-488
Author(s):  
K. Baranowska ◽  
L. Kłyszejko-Stefanowicz ◽  
W. Maciejewska-Potapczyk
Keyword(s):  
Nicotiana Tabacum ◽  
Nucleic Acids ◽  
Dna Content ◽  
Callus Tissue ◽  
Tumour Tissue ◽  
Phosphorus Compounds ◽  
P Fractions ◽  
P Fraction ◽  
Fraction Increase ◽  
Callus Tissues

Phosphorus campounds in 2-, 4-, S- and 8-week old cultures of tumour and callus tissues of Nicotiana tabacum were fractionated by the method of Schmidt and Thannhauser (1945) modified toy Niemierko (1953). Much higher contents of P fractions were found in tumours as compared with callus tissues DNA content in tumour tissue increased during 8 weeks. The contents of the acid-soluble P fraction and RNA + pbosphoprotein fraction increase until 6 -weeks, then it decreases. Callus tissue however shows a decreased nucleic acids P fraction after 4 weeks. The remaining fractions of this tissue increase slightly in this period but they decrease after 6 and 8 weeks.

scholarly journals Nucleic acids synthesis in normal and tumorous tobacco calluses treated with hydroxyurea in vitro

2015 ◽  
Vol 48 (2) ◽  
pp. 131-145
Author(s):  
Aldona Rennert
Keyword(s):  
Nucleic Acids ◽  
Rna Synthesis ◽  
Tumour Tissue

In normal and tumorous tobacco calluses treated with hydroxyurea RNA synthesis was investigated with the use of NaH<sub>2</sub><sup>32</sup>PO<sub>4</sub> as precursor and the method of fractionation on MAK columns. Low inhibitor concentrations show a specific action, and the influence of this compound on the RNA of the two tissues is different. In tumour tissue the population of the rapidly labelled RNA localized in region IV of the elution profiles exhibits remarkable resistance to hydroxyurea (HU). Moreover, disturbances of equilibrium between synthesis and degregation of heavier RNA, indicating inhibition of its degradation, are observed.

scholarly journals Physiological and biochemical effects of morphactin IT 3233 on callus and tumour tissues of Nicotiana tabacum L. cultured in vitro. IV. IAA oxidase activity. Oat coleoptile biotest

2015 ◽  
Vol 43 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Z. Chirek
Keyword(s):  
Oxidase Activity ◽  
Callus Tissue ◽  
Tissue Growth ◽  
Tumour Tissue ◽  
Iaa Oxidase ◽  
Nicotiana Tabacum L ◽  
Coleoptile Elongation ◽  
Short Time ◽  
Physiological And Biochemical

IAA oxidase activity in callus and tumour tissue of tobacco subjected to the action of morphactin IT 3233 for shorter and longer periods was determined. Control tumour tissue shows an activity higher by about 40 per cent as compared with that of callus tissue. Morphactin applied for a short time (24-h incubation) does not change the activity of the enzyme. When application is prolonged, a considerable enhancement (up to 140%) of the enzyme activity in callus tissue is observed in dependence on the morphactin concentration. In tumour tissues the activity is stimulated by 45 per cent as compared to control. Oat coleoptile elongation growth induced by IAA is limited to 40 per cent when morphactin is added in the concentrations used for tobacco tissue cultures. The possibility of the morphactin action on tissue growth via IAA metabolism is discussed.

scholarly journals Attempts at DNP isolation from callus and tumour tissues of Nicotiana tabacum

2015 ◽  
Vol 40 (2) ◽  
pp. 371-376
Author(s):  
L. Kłyszejko-Stefanowicz ◽  
A. Rennert ◽  
W. Mazurczyk ◽  
M. Wysocki ◽  
W. Potaczyk
Keyword(s):  
Nicotiana Tabacum ◽  
Callus Tissue ◽  
Preliminary Investigation ◽  
Tumour Tissue ◽  
Mean Values ◽  
Callus Tissues

This paper deals with a preliminary investigation of ONP originating from tumour and callus tissues of Nicotiana tabacum. DNP preparations from tumour tissue contained on the average: 54.88% of protein, 38.4% of DNA and 2,9% of RNA. The N/P ratio value fluctuated within the limits 4.8—5.8. The respective mean values for DNP preparations from callus tissue were: 57.3; 21.6 and 13.l%. The NAP ratio was 4.7-6.6.

scholarly journals The effect of benzene and its Chlorella vulgaris metabolites on the growth of tobacco tissue

2014 ◽  
Vol 64 (4) ◽  
pp. 366-370
Author(s):  
E. Szczepkowska-Kuźnicka ◽  
W. Wardas
Keyword(s):  
Nicotiana Tabacum ◽  
Toxic Effect ◽  
Chlorella Vulgaris ◽  
Microscopic Examination ◽  
Callus Tissue ◽  
Tissue Growth ◽  
Tobacco Callus ◽  
Cultivation Medium

The influence of benzene, phenol and catechol on the tobacco callus tissue (<i>Nicotiana tabacum</i> L. cv. Wisconsin No. 38) cultivated <i>in vitro</i> was studied. Although inhibition of callus tissue growth in relation to the control was observed in all variants, the benzene-induced inhibition was the most significant. When catechol and phenol were added to the cultivation medium together, callus tissue growth was similar to that of the control. The addition of catechol to the medium containing phenol presumably reduces the toxic effect of phenol on the callus tissue examined. Callus tissue growing in the presence of phenol, benzene and cathechol became senescent more rapidly than in the control. Moreover microscopic examination revealed significant changes in the callus structure.

MAMMARY NUCLEIC ACIDS AND PITUITARY PROLACTIN SECRETION DURING PROLONGED LACTATION IN MICE

1976 ◽  
Vol 70 (3) ◽  
pp. 389-395 ◽  
Author(s):  
H. NAGASAWA ◽  
R. YANAI
Keyword(s):  
Mammary Gland ◽  
Dna Content ◽  
Rna Synthesis ◽  
Prolactin Secretion ◽  
Nucleic Acid Content ◽  
Plasma Prolactin ◽  
Litter Weight ◽  
Dna And Rna ◽  
Pituitary Prolactin

SUMMARY Lactation was prolonged until 61 days by repeated renewal of litters every week after day 12 in primiparous C3H/He strain mice. On days 12, 19, 40 and 61 of lactation, litters were removed for 5 h and after 1 h of resuckling the synthesis of DNA and RNA in the mammary gland was estimated by the incorporation of [3H]thymidine and [14C]uridine into mammary DNA and RNA in vitro respectively. Mammary nucleic acid content and pituitary and plasma levels of prolactin were also assayed. Nulliparous mice were similarly treated on day 19 of pregnancy. The percentage gain in litter weight per week was highest between days 5 and 12 of lactation, declined until days 26–33 and became steady thereafter. Mammary DNA synthesis was extremely high on day 19 of pregnancy, decreased on day 12 of lactation to less than one-fifteenth of that on day 19 of pregnancy and increased linearly thereafter. Changes in mammary DNA content were not so marked, but DNA content was high on days 12 and 19 of lactation. RNA synthesis was highest on day 19 of pregnancy, abruptly decreased on days 12 and 19 of lactation and increased again with the advance of lactation. Mammary RNA content, RNA:DNA and 14C:3H ratios increased from day 19 of pregnancy to days 12–19 of lactation and decreased on days 40 and 61. While the pituitary levels of prolactin were almost constant during lactation, they were significantly higher than those on day 19 of pregnancy. There were only slight differences in plasma prolactin levels at any stage.

5α-DIHYDROTESTOSTERONE AND THE SYNTHESIS OF RNA IN THE RAT VENTRAL PROSTATE IN VITRO

1971 ◽  
Vol 68 (3) ◽  
pp. 567-575 ◽  
Author(s):  
Ingrid Alfheim ◽  
Olav Unhjem
Keyword(s):  
Incubation Medium ◽  
Rna Synthesis ◽  
Ventral Prostate ◽  
Isolated Nuclei ◽  
Quantitative Effect ◽  
Rat Ventral Prostate

ABSTRACT The effect of 5α-dihydrotestosterone on RNA synthesis in the rat ventral prostate has been studied in vitro. The steroid was added to the incubation medium in a concentration of 10−6 m. Experiments have been performed with slices, homogenates, crude nuclear preparations and isolated nuclei from the rat ventral prostate. The results appear to indicate that there is no immediate quantitative effect of the steroid on RNA synthesis when administered in vitro.

scholarly journals Purine metabolism in mesophyll protoplasts of tobacco (Nicotiana tabacum) leaves

1980 ◽  
Vol 186 (1) ◽  
pp. 343-350 ◽  
Author(s):  
J Barankiewicz ◽  
J Paszkowski
Keyword(s):  
Nucleic Acid ◽  
Nicotiana Tabacum ◽  
Nucleic Acids ◽  
Metabolic Pathways ◽  
Rna Synthesis ◽  
Acid Fraction ◽  
Purine Nucleotide ◽  
Tobacco Leaf ◽  
Mesophyll Protoplasts ◽  
Leaf Mesophyll

The overall metabolism of purines was studied in tobacco (Nicotiana tabacum) mesophyll protoplasts. Metabolic pathways were studied by measuring the conversion of radioactive adenine, adenosine, hypoxanthine and guanine into purine ribonucleotides, ribonucleosides, bases and nucleic acid constituents. Adenine was extensively deaminated to hypoxanthine, whereupon it was also converted into AMP and incorporated into nucleic acids. Adenosine was mainly hydrolysed to adenine. Inosinate formed from hypoxanthine was converted into AMP and GMP, which were then catabolized to adenine and guanosine respectively. Guanine was mainly deaminated to xanthine and also incorporated into nucleic acids via GTP. Increased RNA synthesis in the protoplasts resulted in enhanced incorporation of adenine and guanine, but not of hypoxanthine and adenosine, into the nucleic acid fraction. The overall pattern of purine-nucleotide metabolic pathways in protoplasts of tobacco leaf mesophyll is proposed.

scholarly journals Physiological and biochemical effects of morphactin IT 3233 on callus and tumour tissues of Nicotiana tabacum L. cultured in vitro. I. Growth and nitrogen compounds content

2015 ◽  
Vol 43 (1) ◽  
pp. 59-70
Author(s):  
Z. Chirek
Keyword(s):  
Nicotiana Tabacum ◽  
Callus Tissue ◽  
Dry Mass ◽  
Tissue Growth ◽  
Nitrogen Compounds ◽  
Nitrogen Pool ◽  
Biochemical Effects ◽  
Nicotiana Tabacum L ◽  
Physiological And Biochemical

A significant limitation of callus tissue growth was noted in <i>Nicotiana tabacum</i> L. cultured in vitro under the influence of morphactin IT 3233 applied in 10 - 40 mg/dm<sup>3</sup> concentrations. Growth inhibition was associated with an increase in dry mass content and the contribution of protein N to the nitrogen, pool. Tumour tissue of tobacco under the same conditions reacted weaker to morphactin. Growth limitation reached 25 per cent as compared with the control, dry mass and nitrogen compounds content showed only slight variations. It is supposed that the different sensitivity of both types of tissues to morphactin is connected with their different hormonal metabolism.

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