scholarly journals Fractionation of phosphorus compounds in tumor and callus tissues of Nicotiana tabacum

2015 ◽  
Vol 41 (4) ◽  
pp. 481-488
Author(s):  
K. Baranowska ◽  
L. Kłyszejko-Stefanowicz ◽  
W. Maciejewska-Potapczyk
Keyword(s):  
Nicotiana Tabacum ◽  
Nucleic Acids ◽  
Dna Content ◽  
Callus Tissue ◽  
Tumour Tissue ◽  
Phosphorus Compounds ◽  
P Fractions ◽  
P Fraction ◽  
Fraction Increase ◽  
Callus Tissues

Phosphorus campounds in 2-, 4-, S- and 8-week old cultures of tumour and callus tissues of Nicotiana tabacum were fractionated by the method of Schmidt and Thannhauser (1945) modified toy Niemierko (1953). Much higher contents of P fractions were found in tumours as compared with callus tissues DNA content in tumour tissue increased during 8 weeks. The contents of the acid-soluble P fraction and RNA + pbosphoprotein fraction increase until 6 -weeks, then it decreases. Callus tissue however shows a decreased nucleic acids P fraction after 4 weeks. The remaining fractions of this tissue increase slightly in this period but they decrease after 6 and 8 weeks.

scholarly journals Influence of hydroxyurea on nucleic acids content and 3H-uridine incorporation in callus and tumorous tobacco tissues cultured in vitro

2015 ◽  
Vol 44 (3) ◽  
pp. 393-405
Author(s):  
A. Bielecka
Keyword(s):  
Nicotiana Tabacum ◽  
Nucleic Acids ◽  
Dna Content ◽  
Incubation Medium ◽  
Rna Synthesis ◽  
Callus Tissue ◽  
Tumour Tissue ◽  
Inhibitory Influence ◽  
Tumor Tissues

In callus and tumor tissues of <i>Nicotiana tabacum</i> cultured for 39 days in media supplemented with various concentrations of hydroxyurea (1.3 x 10<sup>-4</sup> M - 1.3 x 10<sup>-3</sup> M) a decrease of DNA content (ca. 24 per cent in callus tissue and ca. 23 per cent in tumour tissue) and a decrease of RNA content (over 10 per cent and ca. 9 per cent in callus and tumour tissue, respectively) was observed. The autoradiographic method showed that a long-lasting action of this com-pound inhibits RNA synthesis. A stronger inhibitory influence of hydroxyurea upon incorporation of 3H-uridine from the incubation medium was revealed.

scholarly journals Attempts at DNP isolation from callus and tumour tissues of Nicotiana tabacum

2015 ◽  
Vol 40 (2) ◽  
pp. 371-376
Author(s):  
L. Kłyszejko-Stefanowicz ◽  
A. Rennert ◽  
W. Mazurczyk ◽  
M. Wysocki ◽  
W. Potaczyk
Keyword(s):  
Nicotiana Tabacum ◽  
Callus Tissue ◽  
Preliminary Investigation ◽  
Tumour Tissue ◽  
Mean Values ◽  
Callus Tissues

This paper deals with a preliminary investigation of ONP originating from tumour and callus tissues of Nicotiana tabacum. DNP preparations from tumour tissue contained on the average: 54.88% of protein, 38.4% of DNA and 2,9% of RNA. The N/P ratio value fluctuated within the limits 4.8—5.8. The respective mean values for DNP preparations from callus tissue were: 57.3; 21.6 and 13.l%. The NAP ratio was 4.7-6.6.

scholarly journals Physiological and biochemical effects of morphactin IT 3233 on callus and tumour tissues of Nicotiana tabacum L. cultured in vitro. II. Protein synthesis and respiratory activity

2015 ◽  
Vol 43 (1) ◽  
pp. 71-80
Author(s):  
Z. Chirek
Keyword(s):  
Nicotiana Tabacum ◽  
Callus Tissue ◽  
Respiratory Activity ◽  
Tissue Growth ◽  
The Other ◽  
Biochemical Effects ◽  
Nicotiana Tabacum L ◽  
Callus Tissues ◽  
Physiological And Biochemical

It was found that the inhibition of callus tissue growth in <i>Nicotiana tabacum</i> L. cultured <i>in vitro</i> by the application of morphactin IT 3233 is associated with a rise of the protein level in spite of a 50 per cent depression of its synthesis. Respiratory activity of these tissues is also lowered. Tumour tissues, on the other hand, show only light changes in the parameters studied. It would seem that morphactin depresses a metabolic activity in the callus tissues, and probably causes deposition of a, large quantity of metabolically inactive proteins.

scholarly journals Influence of Application of Organic Residues of Different Biochemical Quality on Phosphorus Fractions in a Tropical Sandy Soil

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 248
Author(s):  
Tanabhat-Sakorn Sukitprapanon ◽  
Metawee Jantamenchai ◽  
Duangsamorn Tulaphitak ◽  
Nattaporn Prakongkep ◽  
Robert John Gilkes ◽  
...  
Keyword(s):  
Leaf Litter ◽  
Sandy Soil ◽  
Organic Residue ◽  
Residue Quality ◽  
Organic Residues ◽  
P Fractions ◽  
Residual P ◽  
Extractable P ◽  
P Fraction ◽  
Labile P

Understanding phosphorus (P) dynamics in tropical sandy soil treated with organic residues of contrasting quality is crucial for P management using organic amendments. This research determined P fractions in a tropical sandy soil under the application of organic residues of different quality, including groundnut stover (GN), tamarind leaf litter (TM), dipterocarp leaf litter (DP), and rice straw (RS). The organic residues were applied at the rate of 10 t DM ha−1 year−1. The P fractions were examined by a sequential extraction procedure. Organic residue application, regardless of residue quality, resulted in P accumulation in soils. For unamended soil, 55% of total P was mainly associated with Al (hydr)oxides. Organic residue application, regardless of residue quality, diminished the NH4F-extractable P (Al-P) fraction, but it had a nonsignificant effect on NaOH-extractable P (Fe-P). The majority of Al-P and Fe-P fractions were associated with crystalline Al and Fe (hydr)oxides. NH4Cl-extractable P (labile P), NaHCO3-extractable P (exchangeable P and mineralizable organic P), HCl-extractable P (Ca-P), and residual P fractions in soil were significantly increased as a result of the incorporation of organic residues. The application of organic residues, particularly those high in ash alkalinity, increase soil pH, labile P, and Ca-P fractions. In contrast, applications of residues high in lignin and polyphenols increase residual P fraction, which is associated with organo-mineral complexes and clay mineral kaolinite.

Growth Inhibition in Tobacco (Nicotiana tabacum) Callus by 2,6-Dinitroaniline Herbicides and Protection by D-α-tocopherol Acetate

Weed Science ◽  
1978 ◽  
Vol 26 (6) ◽  
pp. 527-530 ◽  
Author(s):  
J. B. Huffman ◽  
N. D. Camper
Keyword(s):  
Regression Analysis ◽  
Nicotiana Tabacum ◽  
Callus Tissue ◽  
Linear Regression Analysis ◽  
Tissue Growth ◽  
Tissue Cultures ◽  
Fresh Weight ◽  
Structural Variations ◽  
Tocopherol Acetate ◽  
Dinitroaniline Herbicides

Tobacco(Nicotiana tabacumL. ‘X-73’) callus tissue cultures were used in a bioassay system for determining the effect of the following substituted 2,6-dinitroaniline herbicides on growth: trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine); oryzalin (3,5-dinitro-N4,N4-dipropylsulfanilamide); benefin(N-butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-toluidine); AC 92390(N-sec-butyl-2,6-dinitro-3,4-xylidine); penoxalin [N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine]; GS-38946(N-ethyl-N-tetrahydrofurfuryl-4-trifluoromethyl-2,6-dinitroaniline); chlornidine [N,N-di(2-chloroethyl)-4-methyl-2,6-dinitroaniline]; nitralin [4-(methylsulfonyl)2,6-dinitro-N,N-dipropylaniline]; dinitramine(N4,N4-diethyl-α,α,α-trifluoro-3,5-dinitrotoluene-2,4-diamine); isopropalin (2,6-dinitro-N,N-dipropylcumidine), and profluralin [N(cyclopropylmethyl)-α,α,α-trifluoro-2,6-dinitro-N-propyl-p-toluidine]. The molar concentration required to inhibit fresh weight gain by 50% (I50) was determined by using linear regression analysis on data from a range of five concentrations of each chemical. I50values did not correlate with structural variations or available physical constants. Herbicides listed in order of increasing activity are AC 92390< GD-38946<profluralin = isopropalin<benefin = chlornidine = trifluralin = nitralin<oryzalin = penoxalin < dinitramine. Exogenously applied D-α-tocopherol acetate at 100 times the I50concentrations decreased the inhibition of tissue growth by the herbicides.

Growth substance requirements and major lipid constituents of tissue cultures of Euphorbia esula and E. cyparissias

1972 ◽  
Vol 50 (4) ◽  
pp. 723-726 ◽  
Author(s):  
T. T. Lee ◽  
A. N. Starratt
Keyword(s):  
Callus Tissue ◽  
Defined Medium ◽  
Growth Substance ◽  
Tissue Growth ◽  
Euphorbia Esula ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Callus Tissues ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Root Tissues

The root tissues of Euphorbia esula and E. cyparissias form callus on chemically defined medium. Both species require an exogenous supply of auxin for growth, but the appearance and color of the tissue and their responses to kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D) and indoleacetic acid (IAA) are different. The tissue growth is more satisfactory with α-naphthaleneacetic acid (NAA) than with 2,4-D, IAA, or 4-amino-3,5,6-trichloropicolinic acid (picloram). Gibberellic acid has no effect. The callus tissues of E. esula become intensely green under light but are not autotrophic.Triglycerides, palmitic acid, and β-sitosterol are the major lipid constituents of the callus tissue of E. esula. Chromatographic analysis reveals no significant differences in the composition of extracts from the non-green and green tissues. Long-chain aldehydes, alcohols, and triterpenes found in the plant are not detected in the cultures.

scholarly journals Physiological and biochemical effects of morphactin IT 3233 on callus and tumour tissues of Nicotiana tabacum L. cultured in vitro. IV. IAA oxidase activity. Oat coleoptile biotest

2015 ◽  
Vol 43 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Z. Chirek
Keyword(s):  
Oxidase Activity ◽  
Callus Tissue ◽  
Tissue Growth ◽  
Tumour Tissue ◽  
Iaa Oxidase ◽  
Nicotiana Tabacum L ◽  
Coleoptile Elongation ◽  
Short Time ◽  
Physiological And Biochemical

IAA oxidase activity in callus and tumour tissue of tobacco subjected to the action of morphactin IT 3233 for shorter and longer periods was determined. Control tumour tissue shows an activity higher by about 40 per cent as compared with that of callus tissue. Morphactin applied for a short time (24-h incubation) does not change the activity of the enzyme. When application is prolonged, a considerable enhancement (up to 140%) of the enzyme activity in callus tissue is observed in dependence on the morphactin concentration. In tumour tissues the activity is stimulated by 45 per cent as compared to control. Oat coleoptile elongation growth induced by IAA is limited to 40 per cent when morphactin is added in the concentrations used for tobacco tissue cultures. The possibility of the morphactin action on tissue growth via IAA metabolism is discussed.

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