5α-DIHYDROTESTOSTERONE AND THE SYNTHESIS OF RNA IN THE RAT VENTRAL PROSTATE IN VITRO

1971 ◽  
Vol 68 (3) ◽  
pp. 567-575 ◽  
Author(s):  
Ingrid Alfheim ◽  
Olav Unhjem
Keyword(s):  
Incubation Medium ◽  
Rna Synthesis ◽  
Ventral Prostate ◽  
Isolated Nuclei ◽  
Quantitative Effect ◽  
Rat Ventral Prostate

ABSTRACT The effect of 5α-dihydrotestosterone on RNA synthesis in the rat ventral prostate has been studied in vitro. The steroid was added to the incubation medium in a concentration of 10−6 m. Experiments have been performed with slices, homogenates, crude nuclear preparations and isolated nuclei from the rat ventral prostate. The results appear to indicate that there is no immediate quantitative effect of the steroid on RNA synthesis when administered in vitro.

5α-DIHYDROTESTOSTERONE AND THE SYNTHESIS OF RNA IN THE RAT VENTRAL PROSTATE IN VITRO. II

1973 ◽  
Vol 73 (1) ◽  
pp. 189-195 ◽  
Author(s):  
Ingrid Alfheim ◽  
Bjørg Fjell
Keyword(s):  
Gel Electrophoresis ◽  
Rna Synthesis ◽  
Gradient Centrifugation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Extraction Procedure ◽  
Ventral Prostate ◽  
Double Labelling ◽  
Phenol Extraction ◽  
Rat Ventral Prostate

ABSTRACT A double labelling technique has been used to study RNA synthesis in explanted tissues obtained from ventral prostates of castrated rats, incubated with or without 5α-dihydrotestosterone in the medium. RNA was isolated by a phenol extraction procedure, and analysed by sucrose gradient centrifugation and polyacrylamide gel electrophoresis. No specific hormonal response could be detected.

EFFECT OF 17α-METHYL-β-NORTESTOSTERONE (SK & F 7690) ON THE BINDING IN VITRO OF 5α-DIHYDROTESTOSTERONE TO MACROMOLECULAR COMPONENTS FROM THE RAT VENTRAL PROSTATE

1971 ◽  
Vol 66 (2) ◽  
pp. 352-356 ◽  
Author(s):  
Kjell J. Tveter
Keyword(s):  
Tissue Culture ◽  
Protein Complex ◽  
Incubation Medium ◽  
Culture Medium ◽  
Tissue Culture Medium ◽  
Male Rats ◽  
Ventral Prostate ◽  
Nuclear Fraction ◽  
Rat Ventral Prostate

ABSTRACT Slices from prostate glands of castrated male rats were incubated with [3H] 5α-dihydrotestosterone in Eagle's tissue culture medium. The labelled androgen was associated with androphilic macromolecules both in the prostatic cytosol and the nuclei. The addition of the anti-androgenic compound, 17α-methyl-β-nortestosterone (SK & F 7690) to the incubation medium inhibited the formation of the nuclear 5α-dihydrotestosterone-protein complex, and markedly reduced the cytosol 5α-dihydrotestosterone-protein complex. Likewise, the uptake of [3H] 5α-dihydrotestosterone by the prostatic nuclear fraction was reduced by about 40%.

FURTHER STUDIES ON A SOLUBLE ANDROGEN-MACROMOLECULAR COMPLEX FROM THE RAT VENTRAL PROSTATE

1970 ◽  
Vol 65 (3) ◽  
pp. 517-524 ◽  
Author(s):  
Olav Unhjem
Keyword(s):  
Hydrogen Atom ◽  
Keto Group ◽  
Ventral Prostate ◽  
Metabolic Inhibitors ◽  
Macromolecular Complex ◽  
Structural Requirements ◽  
Macromolecular Complexes ◽  
Rat Ventral Prostate

ABSTRACT The ability of various steroids and metabolic inhibitors to influence the binding of androgen to soluble macromolecules in the rat ventral prostate was evaluated in vitro. The results obtained revealed some structural requirements of steroids for binding to the macromolecules. An androstane skeleton with the α-configuration of the hydrogen atom at position 5 seemed to be essential for binding as well as a keto group at position 3. N-ethylmaleimide, Na-iodoacetate and p-hydroxymercuribenzoate inhibited the binding of androgen to macromolecules. The androgen-macromolecular complexes appeared to be rather stable at temperatures below 5°C.

scholarly journals Incorporation of Testosterone and 5α-Dihydrotestosterone into Rat Ventral Prostate in vitro

1970 ◽  
Vol 17 (6) ◽  
pp. 453-458 ◽  
Author(s):  
JUN SHIMAZAKI ◽  
JIN SATO ◽  
HISAKO NAGAI ◽  
KEIZO SHIDA
Keyword(s):  
Ventral Prostate ◽  
Rat Ventral Prostate

scholarly journals Initiation and elongation of polyribonucleotide chains on rat ventral-prostate chromatin transcribed by homologous ribonucleic acid polymerase B.

1977 ◽  
Vol 166 (2) ◽  
pp. 189-198 ◽  
Author(s):  
P Thomas ◽  
P Davies ◽  
K Griffiths
Keyword(s):  
Rna Polymerase ◽  
Rna Synthesis ◽  
Prostate Gland ◽  
Ventral Prostate ◽  
Control Of Gene Expression ◽  
Rat Ventral Prostate ◽  
Associated Proteins ◽  
Ribonucleoside Triphosphate ◽  
Androgenic Steroids ◽  
Transcriptional Process

The characteristics of initiation of RNA synthesis and the elongation of RNA chains on rat ventral-prostate chromatin by RNA polymerase B were investigated by two methods. 1. Initiation was carried out under low-salt conditions with three ribonucleoside triphosphates, and elongation was begun in the absence of reinitiation by the addition of the fourth ribonucleoside triphosphate and increasing the salt concentration. 2. Stable initiation complexes were formed by preincubation of enzyme with template at 37 degrees C, elongation was started by the addition of all four ribonucleoside triphosphates and reinitiation or spurious RNA synthesis was prevented by rifamycin AF/013. The latter method gave more reliable results. The dependence of those parameters on the androgenic status of the animal was studied. During the first 24h after castration, elongation was mainly affected, whereas after 72h a smaller number of initiation sites for RNA polymerase B on chromatin was evident. Considerable diurnal variations in the various parameters were observed. Changes in the relative concentrations of the chromatin-associated proteins were also observed after castration. In the rat ventral-prostate gland androgenic steroids may not only influence one stage of the transcriptional process, but may affect many factors involved in the control of gene expression.

Cell surface modifications in the epithelium of rat ventral prostate during adaptation to in vitro conditions: An ultrastructural study

In Vitro ◽  
1984 ◽  
Vol 20 (3) ◽  
pp. 216-228 ◽  
Author(s):  
Frederick B. Merk ◽  
Paul W. L. Kwan ◽  
Stanley Spilman ◽  
Louis Terracio ◽  
William H. J. Douglas
Keyword(s):  
Cell Surface ◽  
Ultrastructural Study ◽  
Surface Modifications ◽  
Ventral Prostate ◽  
Rat Ventral Prostate
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