scholarly journals Heterogeneity of the acid phosphatase and ribonuclease from protocorms of the orchids Cymbidium Sw. and changes occurring after treatment with streptomycin

2015 ◽  
Vol 42 (1) ◽  
pp. 133-141 ◽  
Author(s):  
B. Morawiecka ◽  
A. Kubicz ◽  
K. Kukułczanka ◽  
A. Koch ◽  
E. Markefka
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Culture Medium ◽  
Disc Electrophoresis ◽  
Molecular Forms ◽  
Stages Of Development ◽  
Early Stages ◽  
Electrophoretic Patterns ◽  
Culture Growth

The molecular forms of the acid phosphatase and RNase in protocorms of <i>Cymbidium</i> Sw. were studied by disc electrophoresis. The effect of streptomycin added to the culture medium on both enzymes was investigated. Significant changes in enzyme activity and electrophoretic patterns occured after addition of streptomycin at the beginning of culture growth. This indicates that the enzymes are affected by streptomycin in early stages of development of the protocorms.

scholarly journals The activity of acid phosphatase and the level of storage proteins during the early stages of germination of Lupinus luteus L. and Lupinus angustifolius L. seeds

2014 ◽  
Vol 61 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Mariusz Olczak ◽  
Ewa Nizioł ◽  
Wiesława Widłak ◽  
Bronisława Morawiecka
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Protein Level ◽  
Acid Phosphatase Activity ◽  
Storage Proteins ◽  
Lupinus Angustifolius ◽  
Lupinus Luteus ◽  
Molecular Forms ◽  
Early Stages ◽  
Initial Decrease

Changes in the level of protein and in the activity of acid phosphatase in seeds of <i>Lupinus luteus</i> L. and <i>Lupinus angustifolius</i> L. during storage and germination were studied. The protein level decreased during germination, mainly as the result of a fall in the concentration of β-conglutin. After an initial decrease in the activity of acid phosphatase activity during the first five days of germination, the activity of this enzyme began to rise with the appearance of two new molecular forms. No significant effect of gibberelin A3 on the specific ativity of acid phosphatase was observed.

scholarly journals Activity and localization of some hydrolytic enzymes during the development of Iris pseudoacorus endosperm

2015 ◽  
Vol 41 (2) ◽  
pp. 223-233 ◽  
Author(s):  
B. Gabara ◽  
L. Konopska ◽  
M. J. Olszewska
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Enzymatic Activity ◽  
Cell Walls ◽  
Hydrolytic Enzymes ◽  
Stages Of Development ◽  
Early Stages

The changes in the activity of some hydrolytic enzymes during the development of <i>Iris pseudoacorus</i> endosperm were investigated using biochemical and cytochemical methods. In the early stages of development the chalazal pole shows a greater enzymatic activity than the micropylar pole. These differences decline as the seeds mature, and the activity of the studied enzymes becomes lower as the endosperm develops. Considerable activity of ß-galactosidase has been observed at the time of deposition of storage hemi-celluloses in the cell walls of the endosperm. Activity of the cytochemically datectable -hydrolases is localised in granules up to 2μ in diameter. Cytochemical observations in the electron microscope indicate that the activity of acid phosphatase is associated with spherosomes.

Smartphone Based Highly Sensitive Visualized Detection of Acid Phosphatase Enzyme Activity

2021 ◽  
Author(s):  
Rui Li ◽  
Yanan Sun ◽  
Lihua Jin ◽  
Xiaohong Qiao ◽  
Cong Li ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Point Of Care ◽  
Rapid Development ◽  
Practical Application ◽  
Fast Analysis ◽  
Highly Sensitive ◽  
Visualized Detection ◽  
Sensitive Method ◽  
Phosphatase Enzyme

With the rapid development of point-of-care (POC) technologies, the improvement of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for the practical application. In...

scholarly journals Enzyme Histochemical Studies of Adipose Tissue in Porcine Yellow Fat Disease

1974 ◽  
Vol 11 (6) ◽  
pp. 465-476 ◽  
Author(s):  
L. H. J. C. Danse ◽  
W. A. Steenbergen-Botterweg
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Acid Phosphatase ◽  
Nonspecific Esterase ◽  
Cell Structures ◽  
Increased Activity

Adipose tissue of piglets with yellow fat disease had increased activity of nonspecific esterase, 5-nucleotidase, and acid phosphatase. Since these enzymes are associated with different cell structures and damage to these structures can result in increased enzyme activity, they are criteria for pathogenetic study of yellow fat disease.

Comparative leaf development of aerial and aquatic growth forms of Myriophyllum aquaticum

Botany ◽  
2013 ◽  
Vol 91 (7) ◽  
pp. 421-430 ◽  
Author(s):  
M.D. Shafiullah ◽  
Christian R. Lacroix
Keyword(s):  
Apical Meristem ◽  
Epidermal Cells ◽  
The Other ◽  
Developmental Study ◽  
Growth Forms ◽  
Myriophyllum Aquaticum ◽  
Stages Of Development ◽  
Qualitative And Quantitative ◽  
Early Stages ◽  
Other Hand

Myriophyllum aquaticum (Vell.) Verdc. produces two morphologically different forms of leaves based on whether they are aerial or aquatic. The objective of this study was to determine whether there are any similarities or differences between these two growth forms during their early stages of development. A comparative developmental study of aerial and aquatic growth forms of M. aquaticum was conducted from a qualitative and quantitative perspective using a scanning electron microscope. The pattern of leaf and lobe initiation such as their origin and shape were similar in both growth forms until the fourth plastochron (stage P4). Differences between the two growth forms became evident from stage P5 onward, where a larger shoot apical meristem (SAM), elongated epidermal cells, shorter and slightly more numerous lobes, as well as the presence of appendage-like structures characterized aquatic growth forms. On the other hand, aerial growth forms had smaller SAM, bulb-like epidermal cells, and longer and slightly less numerous leaf lobes. Significant differences between growth forms were noted for parameters such as volume of SAM, length of terminal, first, and middle lobes, as well as the length from first to last lobes. The volume of the SAM of aquatic shoot tips was always greater than aerial forms. On the other hand, lobes of aerial forms were always longer than the aquatic counterpart during early stages of development. This study on the development of M. aquaticum shows that the aerial and aquatic growth forms diverge from their early stages of development.

scholarly journals Purification and subunit structure of argininosuccinate lyase from Chlamydomonas reinhardi

1977 ◽  
Vol 167 (1) ◽  
pp. 71-75 ◽  
Author(s):  
R F Matagne ◽  
J P Schlösser
Keyword(s):  
Enzyme Activity ◽  
Crude Extract ◽  
Enzyme Preparation ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Activity Analysis ◽  
Disc Electrophoresis ◽  
Subunit Structure ◽  
Argininosuccinate Lyase

Argininosuccinate lyase (EC 4.3.2.1) was purified by (NH4)2SO4 fractionation, chromatography on DEAE-cellulose and gel filtration on Sephadex G-200. The final enzyme preparation was purified 46-fold compared with the crude extract. Electrophoresis of this preparation revealed three bands, the major one having the enzyme activity. Analysis of the enzyme by gel filtration and by disc electrophoresis (in two different concentrations of acrylamide) gave mol.wts. of 200000 (+/- 15000) and 190000 (+/- 20000) respectively. Treatment with sodium dodecyl sulphate and mercaptoethanol dissociated the enzyme into subunits of mol.wt. 39000 (+/-2000). The results are indicative of the multimeric structure of the enzyme, which is composed of five (perhaps four or six) identical subunits.

XV. On the development of the parasitic isopoda

1878 ◽  
Vol 169 ◽  
pp. 505-521 ◽  
Keyword(s):  
The Body ◽  
The Other ◽  
Stages Of Development ◽  
Early Stages ◽  
Abdominal Appendages ◽  
Pair Of Antennae

The following paper contains an account of observations on the development of the species Cymothoa œstroides and C . parallela of Milne Edwards; but the forms of the young seem to show that several species are really included under these two names. In the early stages of development the only observable difference that exists between the embryos is one of size, but in the later stages they differ very markedly from each other in their external characters. From adult individuals answering the description of C . œstroides I have obtained four varieties of embryos: two with long antennae and two with short.* In the two former the first pair of antennae are but slightly longer than the head, while the second pair are longer than the body; the eyes are small. In one of the varieties thus characterised the abdominal appendages are fringed with long hairs (fig. 20), and in the other they are smooth.

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