Mycoflora of seeds of Bellis perennis and Celosia cristata

Barbara Łacicowa; Irena Kiecan; Danuta Pięta

doi:10.5586/am.1992.005

Mycoflora of seeds of Bellis perennis and Celosia cristata

Acta Mycologica ◽

10.5586/am.1992.005 ◽

2014 ◽

Vol 27 (1) ◽

pp. 41-48

Author(s):

Barbara Łacicowa ◽

Irena Kiecan ◽

Danuta Pięta

Keyword(s):

Alternaria Alternata ◽

Agar Medium ◽

Phoma Exigua ◽

Celosia Cristata

The seeds of Bellis perennis and Celosia cristata were investigated. Agar medium with nutrients was used to isolate the fungi. Alternaria alternata dominated among isolated fungi. The investigation showed that Phoma exigua was more pathogenic to Bellis perennis.

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Micromycetes on Pisum sativum var. arvense

Acta Mycologica ◽

10.5586/am.1997.004 ◽

2014 ◽

Vol 32 (1) ◽

pp. 31-39

Author(s):

Joanna Marcinkowska

Keyword(s):

Pisum Sativum ◽

Botrytis Cinerea ◽

Sclerotinia Sclerotiorum ◽

Alternaria Alternata ◽

Agar Medium ◽

Mycosphaerella Pinodes ◽

Stemphylium Botryosum ◽

Number Of Species ◽

Fusarium Poae ◽

The Common

Seeds of 11 Austrian winter pea genotypes, harvested at Radzików (CEP) in 1993 and 1994, were evaluated for fungi occurrence on Coon's agar medium in Petri plates. Number of species isolated depended on the genotype and year of collection. Alternaria alternata, Stemphylium botryosum were found on all the tested samples and Phoma pinodella and Fusarium poae were also common while Botrytis cinerea, Sclerotinia sclerotiorum and Mycosphaerella pinodes appeared to be common only in 1993. Three species occurred only once. The mycoflora was richer in 1993. The common seed inhabitants usually transmitted higher percentage of fungi than species occuring more seldom.

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Mycoflora of Calendula officinalis L. seeds

Acta Agrobotanica ◽

10.5586/aa.1991.004 ◽

2013 ◽

Vol 44 (1-2) ◽

pp. 49-53

Author(s):

Danuta Pięta

Keyword(s):

Botrytis Cinerea ◽

Alternaria Alternata ◽

Agar Medium ◽

Fusarium Culmorum ◽

Fusarium Avenaceum ◽

Calendula Officinalis ◽

Calendula Officinalis L

The seeds of Calendula offlcinalis harvested in the years 1985-1987 were investigated. Agar medium with nitrients was used to isolate the fungi. As a result of the mycological analysis, 3642 isolates belonglng to 23 species and dark unsporulating mycelium were obtained. Alternaria alternata dominated among the isolated fungi. Moreover, Botrytis cinerea, Fusarium culmorum, Fusarium avenaceum and Sclerotinia sc1erotiorum were obtained from the seeds.

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Survey, survival and control of Alternaria alternata in wheat seeds

Revista Brasileira de Sementes ◽

10.1590/s0101-31222012000300001 ◽

2012 ◽

Vol 34 (3) ◽

pp. 358-365 ◽

Cited By ~ 4

Author(s):

Ricardo Trezzi Casa ◽

Paulo Roberto Kuhnem Junior ◽

Amauri Bogo ◽

Ana Maria Munerati Belani ◽

Jonatha Marcel Bolzan ◽

...

Keyword(s):

Alternaria Alternata ◽

Agar Medium ◽

Wheat Cultivar ◽

Wheat Cultivars ◽

Wheat Seed ◽

Southern Brazil ◽

Air Humidity ◽

Potato Dextrose Agar Medium ◽

Relative Air Humidity ◽

And Control

The fungus Alternaria alternata was quantified in 75 wheat seed samples collected from three different regions of southern Brazil for Cropping and Use Value (CUV) I, II and III. Fungal presence was evaluated in two hundred disinfested seeds per sample before sowing in a potato-dextrose-agar medium + antibiotic (PDA+A). Fungus survival was evaluated every 45 days for 180 days for three seed batches from six wheat cultivars stored in propylene bags in a storehouse, with air temperature varying between 18 to 22 °C and relative air humidity around 60%. The efficacy of carboxin+thiram, difenoconazol, thiram, triadimenol, triticonazol and triticonazol + iprodione fungicides to control A. alternata was determined. A. alternata was detected in all the samples with an incidences of 39.6 %, 38.8% and 35.9% for the CUV I, CUV II and CUV III regions, respectively. The highest mean incidence of the fungus was found in the CUV I region, the coolest and most humid, and was significantly different from the other two regions. The average reduction in A. alternata viability in the wheat cultivar seeds was 49.5% during the 180 days of storage (inter-harvest period), demonstrating that infected seeds are the primary inoculum source for the fungus. The triticonazol + iprodione fungicide mixture efficiently controls A. alternata.

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The studies on ash dying (Fraxinus excelsior L.) in the Włoszczowa Forest Unit stands

Acta Agrobotanica ◽

10.5586/aa.2005.068 ◽

2012 ◽

Vol 58 (2) ◽

pp. 429-440 ◽

Cited By ~ 21

Author(s):

Tadeusz Kowalski ◽

Agata Łukomska

Keyword(s):

Alternaria Alternata ◽

Agar Medium ◽

Fraxinus Excelsior ◽

Malt Agar ◽

Tree Age ◽

Frequency Of Occurrence ◽

Disease Symptoms ◽

Cylindrocarpon Destructans ◽

Fusarium Lateritium ◽

Identification Of Fungi

The studies were carried out in the Włoszczowa Forest Unit, in 9 ash stands differing in respect of age, origin (natural, artificial), site and in the nursery on 3 quarters differing due to a silvicultural method (transplanted and not transplanted) and seedlings age. In each stand an analysis of disease symptoms was carried out on 100 trees (2 - 20 years old stands) or 50 trees (21 - 80 years old stands) growing side by side in central part of the stand, while in the nursery in each block 200 seedlings were analyzed (4 sectors with 50 seedlings each). From the infected seedlings and trees 120 fragments of dead branches, living branches with cankers, and dead roots were taken. Identification of fungi was made on the basis of fructification and over 300 isolations of fungi on malt agar medium. The most frequent disease symptoms in ash stands were: the dead top (34.7% trees), the dying of whole branches (83.5%), the dying of the top of branches (20.1%), the occurrence of healed (36.0%) and unhealed cankers (18.9%) and the slime flux (23.7%) on the trunk, also the chlorosis of leaves (7.5%) and their atrophy (11.2%). Most of the types of disease symptoms appeared irrespectively of the tree age, origin and site, sometimes showing only a difference in the frequency of occurrence. On the seedlings in the nursery the shoot discolouration, healed and unhealed cankers on shoots and necrosis of a part of leaves were recorded most frequently. Disease symptoms occurred more frequently on 4-year-old seedlings in comparison with 3-year-old. In respect of transplanted seedlings the leaves dying was more frequent. Within cankers and on dead tops of shoots the most frequent were: Alternaria alternata, Chalara sp., Cytospora ambiens, Diplodia mutila, Fusarium lateritium, Gloeosporidiella turgida, Phomopsis controversa and Phomopsis scobina. In sparsely found dead roots of living trees appeared mostly: Cryptosporiopsis radicicola, Cylindrocarpon destructans and Phialocephala sp.

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Fungi occurrence on seeds of field pea

Acta Mycologica ◽

10.5586/am.2008.010 ◽

2013 ◽

Vol 43 (1) ◽

pp. 77-89 ◽

Cited By ~ 7

Author(s):

Joanna Marcinkowska

Keyword(s):

Pisum Sativum ◽

Environmental Conditions ◽

Alternaria Alternata ◽

Agar Medium ◽

Field Pea ◽

Mycosphaerella Pinodes ◽

General Level ◽

Stemphylium Botryosum ◽

Fusarium Poae ◽

Ascochyta Pisi

Seeds of four edible cultivars of Pisum sativum and three fodder harvested in 2004-2006 from eight localities, scattered in all region suitable for pea production in Poland, were evaluated for fungi occurrence on CN agar medium in Petri plates. The highest number (27) of species was isolated in 2004, while the lowest (16) in 2006. Number of fungi inhabiting seeds was influenced mainly by environmental conditions of locality and years. Alternaria alternata dominated in each sample of 450 seeds. Species of Penicillium contaminated seeds as the next and infection by Stemphylium botryosum was at similar level. Fusarium poae was the most often occurring species of this genera. Pea specific pathogens: Mycosphaerella pinodes, Phoma pinodella and Ascochyta pisi infected more seeds in 2004 and 2005 than 2006, and at the last season only A. pisi was noted. In general, level of infection by those pathogens was low, reaching on an average only 2.56%, with the highest for A. pisi, and the lowest for M. pinodes.

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