scholarly journals Mycoflora of Calendula officinalis L. seeds

2013 ◽  
Vol 44 (1-2) ◽  
pp. 49-53
Author(s):  
Danuta Pięta

The seeds of <i>Calendula offlcinalis</i> harvested in the years 1985-1987 were investigated. Agar medium with nitrients was used to isolate the fungi. As a result of the mycological analysis, 3642 isolates belonglng to 23 species and dark unsporulating mycelium were obtained. <i>Alternaria alternata</i> dominated among the isolated fungi. Moreover, <i>Botrytis cinerea, Fusarium culmorum, Fusarium avenaceum</i> and <i>Sclerotinia sc1erotiorum</i> were obtained from the seeds.

2014 ◽  
Vol 32 (1) ◽  
pp. 31-39
Author(s):  
Joanna Marcinkowska

Seeds of 11 Austrian winter pea genotypes, harvested at Radzików (CEP) in 1993 and 1994, were evaluated for fungi occurrence on Coon's agar medium in Petri plates. Number of species isolated depended on the genotype and year of collection. <i>Alternaria alternata, Stemphylium botryosum</i> were found on all the tested samples and <i>Phoma pinodella</i> and <i>Fusarium poae</i> were also common while <i>Botrytis cinerea, Sclerotinia sclerotiorum</i> and <i>Mycosphaerella pinodes</i> appeared to be common only in 1993. Three species occurred only once. The mycoflora was richer in 1993. The common seed inhabitants usually transmitted higher percentage of fungi than species occuring more seldom.


2013 ◽  
Vol 44 (1-2) ◽  
pp. 43-47
Author(s):  
Danuta Pięta ◽  
Irena Kiecana

Thirty seed speciments of <i>Viola wittrockiana</i> harvested in the years 1985-1987 were investigated. As a result of mycological analysis, 1808 fungi isolates belonging to 19 species were obtained. Most frequently isolated species was <i>Alternaria alternata</i> whose isolates made 26 % of isolations of all the fungi. Among the fungi isolated particularly from undisinfected seeds the species from the <i>Penicillium</i> kind were dominant. Pathogenic fungi like <i>Botrytis cinerea</i> and <i>Fusarium culmorum</i> were also isolated from the investigated seeds.


2019 ◽  
Vol 12 (1) ◽  
pp. 46-55
Author(s):  
Ján Víglaš ◽  
Petra Olejníková

Abstract This work was focused on the characterization of novel isolate of Trichoderma atroviride O1, found in the forest around the village of Zázrivá (the Northern Slovakia, region Orava). The isolate was identified by sequencing its internal transcribed spacer (ITS) region of rDNA. T. atroviride O1 stimulated the development of lateral roots of model plant Lepidium sativum. Simultaneously, the isolate has proved its high mycoparasitic potential as it displayed the ability to attack colonies of phytopathogenic fungi (Alternaria alternata, Fusarium culmorum, Botrytis cinerea). This isolate produced secondary metabolites, which were isolated and tested for the antimicrobial activity against gram-positive bacteria Staphylococcus epidermidis and Staphylococcus aureus. The growth of these bacteria was suppressed to 10 % and 40 %, respectively. The suppression of the growth of two Candida species was also strong (10 % growth). However, growth parameters of three phytopathogenic fungi (Alternaria alternata, Botrytis cinerea and Fusarium culmorum) were less affected (75 % growth in comparison with the control). Attempts were made to characterize secondary metabolites isolated from T. atroviride O1. Known peptaibols, 20—21 amino acid long, but also shorter peptides, were detected by MALDI-TOF mass spectrometry. Thus, this study demonstrates the plant growth promotion, strong mycoparasitic potential and antimicrobial activity of the isolate T. atroviride O1, which could be in part ascribed to the production of secondary metabolites. This isolate does have a potential in the biocontrol in eco-farming. Further study, particularly, the identification of produced secondary metabolites, is needed.


2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 319-321
Author(s):  
I. Gajda ◽  
H. Kurzawińska ◽  
P. Muras

Stewartia pseudocamelia is one of more attractive of ornamental bushes. The main of decorative quality are: ornamental flowers, non-typical florescence (from end of June to August), original bark and attractive overcolouring of leaves in autumn season. Apart diseases affected Stewartia during vegetation period, the most dangerous are those which infest seedlings. The total number of 132 colonies of fungi were obtained from the sore seedlings of Stewartia. The isolates represented 19 species of thirteen genera. The most dominated of them were fungi of species: Cladosporium cladosporioides, Cylindrocarpon radicicola, Fusarium avenaceum and F. oxysporum. These above-mentioned fungi together with: Phytophthora cinnamomi, Botrytis cinerea, Penicillium spp., Alternaria alternata, Rhizoctonia solani and Pythium debaryanum belonged to the group of dominants and consistued 79.02% of total community.


2017 ◽  
Vol 59 (2) ◽  
Author(s):  
W. Marek Gołębiewski

<p>A series of new 3-substituted isoxazolecarboxamides have been prepared from aldehydes. The key step was a 1,3-dipolar cycloaddition reaction of nitrile oxides to a,b-unsaturated esters and amides. The cycloadditions to amides were mediated by chiral ligands and several products displayed excellent enantioselectivities. Some of the title compounds exhibited good fungicidal activities against <em>Alternaria alternata</em>, <em>Botrytis cinerea</em>, <em>Fusarium culmorum</em>, <em>Phytophthora cactorum</em>, and <em>Rhizoctonia solani</em> strains.</p>


2013 ◽  
Vol 15 (2) ◽  
pp. 208-216 ◽  
Author(s):  
S.M. Mazaro ◽  
H. Fogolari ◽  
A. Wagner Júnior ◽  
I. Citadin ◽  
I. Santos

Foram desenvolvidos três experimentos com o objetivo de avaliar o potencial de preparados a base de calêndula (Calendula officinalis L.) na indução de fitoalexinas em cotilédones de soja, na indução de mecanismos de resistência em frutos de morango, e o efeito fungistático sobre Botrytis cinerea in vitro. O delineamento experimental foi inteiramente casualizado com quatro repetições, para 15 tratamentos resultantes da combinação de três formas de extração (extrato alcoólico, infusão, e maceração) em cinco concentrações (zero; 1,25; 2,5; 5; e 10%). Os resultados demonstram que os preparados de C. officinalis apresentaram capacidade de indução das fitoalexinas gliceolinas em cotilédones de soja. Na aplicação dos preparados em pós-colheita de morangos ocorreu alteração no teor de flavonóides, bem como a atividade da enzima FAL foi estimulada pela aplicação dos extratos; no entanto, não foi constatado o controle de podridão dos frutos. O efeito fungistático foi observado na extração por maceração em todas as suas concentrações reduzindo o crescimento do fungo B. cinerea in vitro sendo que, a partir de 2,5%, observou-se inibição total. A extração por infusão também apresentou resposta positiva na redução do crescimento de B. cinerea, com melhor resposta na concentração de 10% do preparado.


Planta Medica ◽  
2016 ◽  
Vol 82 (05) ◽  
Author(s):  
H Rafiee ◽  
H Naghdi Badi ◽  
A Mehrafarin ◽  
M Taghi Khosravi ◽  
M Tamjidi

2018 ◽  
Vol 72 (4) ◽  
pp. 37-46
Author(s):  
Arkadiusz Telesiński ◽  
Mirosław Onyszko ◽  
Martyna Śnioszek ◽  
Maciej Płatkowski ◽  
Michał Stręk

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