scholarly journals Growth of the entomopathogenic fungi on the medium containing herbicides used in orcharding

2014 ◽  
Vol 25 (2) ◽  
pp. 35-50 ◽  
Author(s):  
Ryszard Miętkiewski ◽  
Anna Sapiecha ◽  
Zofia Miętkiewska
Keyword(s):  
Entomopathogenic Fungi ◽  
Verticillium Lecanii ◽  
Paecilomyces Farinosus

<i>Beauceria bassiana</i> (Bals.) Vuill., <i>Paecilomyces farinosus</i> (Dicks: Fr.) brown et Smith, <i>Verticillium lecanii</i> (Zimm.) Viegas, <i>Conidiobolus thromboides</i> Drechsler, <i>Erynia pieris</i> Li et Humber, <i>E. radicans</i> (Brcf.) Humber were examined on medium to which were added ten herbicides, one fungicide and one insecticide.

scholarly journals Ineraction between some saprophytic and entomopathogenis fungi

2014 ◽  
Vol 23 (1) ◽  
pp. 93-103
Author(s):  
Ryszard Miętkiewski
Keyword(s):  
Entomopathogenic Fungi ◽  
Verticillium Lecanii ◽  
Saprophytic Fungi ◽  
Paecilomyces Farinosus

The studies on interactions between selected entomopathogenic fungi: <i>Beaweria bassiana, Conidiobolus thromboides (= Entomophthora virulenta), Paecilomyces farinosus, Verticillium lecanii</i> and four species of saprophytic fungi were carried out.

scholarly journals The influence of chitin synthesis inhibitors on growth of entomopathogenic fungi in vitro

2014 ◽  
Vol 27 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Anna Sapieha ◽  
Ryszard Miętkiewski
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Verticillium Lecanii ◽  
Chitin Synthesis ◽  
Chitin Synthesis Inhibitors ◽  
Paecilomyces Farinosus

<i>Beauveria bassiana, Paecilomyces farinosus, Verticillium lecanii, Conidiobolus thnmboides, Erynia pieris, E. radicans</i> were examined on medium to which were added three chitin synthesis inhibitors.

scholarly journals Entomopathogenic fungal (Hyphomycetes) collection: assessment of conidial viability

1999 ◽  
Vol 34 (8) ◽  
pp. 1497-1503 ◽  
Author(s):  
Marcos Rodrigues de Faria ◽  
Irene Martins ◽  
Raquel Mello ◽  
Myrian Silvana Tigano
Keyword(s):  
Liquid Nitrogen ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Culture Collection ◽  
Nomuraea Rileyi ◽  
Paecilomyces Farinosus ◽  
Viability Loss ◽  
Conidial Viability

Twenty four strains of the entomopathogenic fungi (Hyphomycetes) Beauveria bassiana, Metarrhizium anisopliae, Nomuraea rileyi, Paecilomyces farinosus, P. fumosoroseus and P. lilacinus, maintained in the culture collection of Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia (Cenargen) and preserved by lyophilization and in liquid nitrogen, had their conidial viability assessed. Germination rates of 16- to 84-month-old cultures stored in liquid nitrogen decreased, on average, less than 13.3%. For 29- to 49-month-old cultures preserved by lyophilization, the viability loss ranged, on average, from 28.6 to 94.5%. The results demonstrated the efficiency of the tested methods, especially liquid nitrogen, in preserving the viability of entomopathogenic fungi.

scholarly journals Entomopathogenic fungi isolated in the vicinity of Szczecin

2014 ◽  
Vol 33 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Ryszard Miętkiewicz ◽  
Magdalena Dzięgielewska ◽  
Krystyna Janowicz
Keyword(s):  
Forest Soil ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Dominant Species ◽  
Beech Forest ◽  
Meadow Soil ◽  
Paecilomyces Farinosus ◽  
Opposite Tendency

The spectrum and occurrence of entomopathogenic fungi in litter beech forest. meadow soil and arable soil were evaluated using an "insect bait method". Soil and litter samples taken in autumn and spring from each stand were baited with <i>G. mellonella</i> larvae at 22°C. Six species of entomopathogenic fungi infected these larvae. In the litter <i>Beauveria bassiana</i> was the dominant species. Besides <i>B. bassiana</i>, 23% of <i>G. mellonella</i> larvae were infected by <i>Paecilomyces farinosus</i> in autumn. In the forest soil the dominant entomopathogenic fungus was not found. In this stand the number of infected larvae was the lowest. <i>Metarhizium anisopliae</i> was the dominant species in the meadow soil in both sampling periods. In the soil taken from a rye field<i> P. fumosoroseus</i> and <i>B. bassiana</i> infected <i>G. mellonella</i> larvae almost to the same degree. It was established that <i>B. bassiana</i> and <i>M. anisopliae</i> infected more <i>G. mellonella</i> larvae in spring than in autumn. In the case of <i>P. farinosus</i> and <i>P. fumosoroseus</i> an opposite tendency was observed.

Genetic variability and phylogeny inferred from random amplified polymorphic DNA data reflect life strategy of entomopathogenic fungi

2000 ◽  
Vol 78 (9) ◽  
pp. 1150-1155 ◽  
Author(s):  
Miroslav Oborník ◽  
Marek Klíc ◽  
Lukáš Zizka
Keyword(s):  
Genetic Variability ◽  
Entomopathogenic Fungi ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Major Influence ◽  
Life Strategy ◽  
Verticillium Lecanii ◽  
Paecilomyces Fumosoroseus ◽  
Neighbor Joining

Our analysis of the genetic variability and phylogenetic relationships among the isolates of mitosporic entomopathogenic fungi Paecilomyces fumosoroseus (Wize) Brown & Smith (10 isolates), Paecilomyces farinosus (Holm ex S.F. Gray) Brown & Smith (3 isolates), Paecilomyces lilacinus (Thom) Samson (1 isolate), and Verticillium lecanii (Zimmerman) Viegas (6 isolates) was based on the use of random amplified polymorphic DNA (RAPD) markers. Neighbor-joining analysis characterized P. fumosoroseus and V. lecanii as monophyletic taxa. The analysis also supported monophyly of the genus Paecilomyces. We have used the distribution of genetic distances to demonstrate the genetic variability within studied species, in Paecilomyces spp. (Hyphomycetes) and Aschersonia spp. (Coelomycetes) in particular. We conclude that the RAPD-based analysis is useful for the reconstruction of intraspecific and interspecific relationships of the entomopathogenic fungi. Moreover, it can be employed to study the intergeneric relationships, especially in the case of closely related genera of the hyphomycetous entomopathogenic fungi. We propose that RAPD-based phylogeny and genetic variability reflect their life strategy. In our opinion, the mobility of spores represents a factor with major influence on the evolution of these entomopathogenic fungi.Key words: Paecilomyces, Verticillium, Aschersonia, RAPD, phylogeny, genetic variability.

scholarly journals Presence of Toxic Substance in Fungus Bodies of the Entomopathogenic Fungi, Beauveria bassiana and Verticillium lecanii

1978 ◽  
Vol 13 (2) ◽  
pp. 97-102 ◽  
Author(s):  
Shigeo MURAKOSHI ◽  
Masakatsu ICHINOE ◽  
Akinori SUZUKI ◽  
Masaharu KANAOKA ◽  
Akira ISOGAI ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Toxic Substance ◽  
Verticillium Lecanii

Phylogeny of mitosporic entomopathogenic fungi: Is the genus Paecilomyces polyphyletic?

2001 ◽  
Vol 47 (9) ◽  
pp. 813-819 ◽  
Author(s):  
Miroslav Oborník ◽  
Milan Jirku ◽  
David Dolezel
Keyword(s):  
Ribosomal Rna ◽  
Entomopathogenic Fungi ◽  
Large Subunit ◽  
Sister Group ◽  
Small Subunit ◽  
Verticillium Lecanii ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Large Subunit Rrna ◽  
Rna Genes

We analyzed sequences of the divergent domain at the 5' end of the large subunit rRNA gene from the mitosporic entomopathogenic fungi Paecilomyces sp., Paecilomyces fumosoroseus, Paecilomyces farinosus, Paecilomyces lilacinus, Verticillium lecanii, Verticillium psalliotae, Beauveria bassiana, Aschersonia sp., Aschersonia placenta, ascomycetous Cordyceps sp., and Cordyceps militaris. Phylogenetic analysis showed P. fumosorseus as the best characterized out of the analyzed species with the B. bassiana clade as its sister group. Two of the P. farinosus isolates were invariably placed within the Verticillium cluster, which also contained C. militaris. The only analyzed P. lilacinus isolate appeared on the root of the hyphomycetous fungi and was characterized as the most distinct from all the hyphomycetous fungi tested. Polyphyly of the genus Paecilomyces was well supported by the Kishino-Hasegawa test. In all trees based on the small subunit rRNA gene sequences obtained from the GenBank(tm), V. lecanii, V. psalliotae, P. fumosoroseus, P. tenuipes and B. bassiana form, together with that of C. militaris, the best supported cluster in the tree. The rest of Cordyceps spp. constitute a distinct clade. Phylogenetic relationships derived from both tested DNA regions show polyphyly of the genus Paecilomyces and close relationships among entomopathogenic species of the genera Verticillium, Paecilomyces, and Beauveria.Key words: Paecilomyces, Verticillium, Beauveria, Aschersonia, entomopathogenic fungi, molecular phylogeny, ribosomal RNA genes.

scholarly journals Temperature requirements of four entomopathogenic fungi

2014 ◽  
Vol 29 (1) ◽  
pp. 109-120 ◽  
Author(s):  
Ryszard Miętkiewski ◽  
Cezary Tkaczuk ◽  
Mieczysław Żurek ◽  
L. P. Van der Geest
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Paecilomyces Farinosus ◽  
Temperature Requirements

Infection potential of <i>Beauvaria bassiana, Metarhizium anisopliae, Paecilomyces farinosus</i> and <i>P. fumosoroseus</i> to <i>G. mellonella</i> larvae in pine litter was established. The growth of these fungi on the Czapek's Dox medium exposed to different temperature was determined.

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