scholarly journals Temperature requirements of four entomopathogenic fungi

2014 ◽  
Vol 29 (1) ◽  
pp. 109-120 ◽  
Author(s):  
Ryszard Miętkiewski ◽  
Cezary Tkaczuk ◽  
Mieczysław Żurek ◽  
L. P. Van der Geest
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Paecilomyces Farinosus ◽  
Temperature Requirements

Infection potential of <i>Beauvaria bassiana, Metarhizium anisopliae, Paecilomyces farinosus</i> and <i>P. fumosoroseus</i> to <i>G. mellonella</i> larvae in pine litter was established. The growth of these fungi on the Czapek's Dox medium exposed to different temperature was determined.

scholarly journals Entomopathogenic fungi isolated in the vicinity of Szczecin

2014 ◽  
Vol 33 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Ryszard Miętkiewicz ◽  
Magdalena Dzięgielewska ◽  
Krystyna Janowicz
Keyword(s):  
Forest Soil ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Dominant Species ◽  
Beech Forest ◽  
Meadow Soil ◽  
Paecilomyces Farinosus ◽  
Opposite Tendency

The spectrum and occurrence of entomopathogenic fungi in litter beech forest. meadow soil and arable soil were evaluated using an "insect bait method". Soil and litter samples taken in autumn and spring from each stand were baited with <i>G. mellonella</i> larvae at 22°C. Six species of entomopathogenic fungi infected these larvae. In the litter <i>Beauveria bassiana</i> was the dominant species. Besides <i>B. bassiana</i>, 23% of <i>G. mellonella</i> larvae were infected by <i>Paecilomyces farinosus</i> in autumn. In the forest soil the dominant entomopathogenic fungus was not found. In this stand the number of infected larvae was the lowest. <i>Metarhizium anisopliae</i> was the dominant species in the meadow soil in both sampling periods. In the soil taken from a rye field<i> P. fumosoroseus</i> and <i>B. bassiana</i> infected <i>G. mellonella</i> larvae almost to the same degree. It was established that <i>B. bassiana</i> and <i>M. anisopliae</i> infected more <i>G. mellonella</i> larvae in spring than in autumn. In the case of <i>P. farinosus</i> and <i>P. fumosoroseus</i> an opposite tendency was observed.

scholarly journals Occurrence of entomopathogenic fungi in arable soil and meadow soil

2014 ◽  
Vol 27 (2) ◽  
pp. 197-203 ◽  
Author(s):  
Ryszard Miętkowski ◽  
Cezary Tkaczyk ◽  
Ludwik Zasada
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Arable Soil ◽  
Meadow Soil ◽  
Paecilomyces Farinosus ◽  
Arable Field ◽  
Laspeyresia Pomonella

The samples of soil for experiment were taken from arable field and meadow. Larvae of <i>Laspeyresia pomonella</i> and <i>Plodia interpuactella</i> were used as an "insect bait" for isolation of entomopathogenic fungi from the soil. <i>Beauveria bassiana, Metarhizium anisopliae, Paecilomyces farinosus</i> and <i>P. fumosoroseus</i> were isolated from both kinds of soil.

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

scholarly journals Growth of entomopathogenic fungi colonies Metarhizium anisopliae (Metchnikoff) Sorokin enriched with termite juice

2021 ◽  
Vol 667 (1) ◽  
pp. 012084
Author(s):  
N Anggraini ◽  
S Suhartono ◽  
A Alfizar ◽  
H Husni ◽  
S Rusdiana ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae

scholarly journals Entomopathogenic fungi as the microbial frontline against the alien Eucalyptus pest Gonipterus platensis in Brazil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Carolina Jordan ◽  
Paula Leite dos Santos ◽  
Leiliane Rodrigues dos Santos Oliveira ◽  
Mauricio Magalhães Domingues ◽  
Bianca Cristina Costa Gêa ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Natural Variation ◽  
Fungal Strains ◽  
Conidial Production ◽  
Laboratory Conditions ◽  
Virulent Strains ◽  
Eucalyptus Plantations ◽  
Infected Adult

AbstractThe eucalyptus snout beetle (ESB), Gonipterus platensis, is endemic to Australia but has become a major invasive, destructive pest of Brazilian eucalyptus plantations. Efforts to develop insecticides based on entomopathogenic fungi against ESB are limited by the lack of known virulent strains. We therefore explored the virulence of indigenous Brazilian strains of major entomopathogenic fungi—Beauveria spp. and Metarhizium anisopliae—against ESB adults. We found widely varying virulence and later capacities for conidial production on infected adult cadavers. Two strains stood out, B. bassiana IBCB-240 and M. anisopliae IBCB-364, as especially lethal for ESB adults under laboratory conditions, sporulated abundantly on infected insects, and also outperformed comparable strains used in commercial mycoinsecticides. Notably, B. bassiana IBCB-240 exhibited lower LT50 values at low inoculum levels (≤ 107 conidia mL−1) and smaller LC50 values than M. anisopliae IBCB-364. Taken together, this study emphasizes natural variation in virulence among indigenous Beauveria and Metarhizium strains against ESB adults and identifies fungal strains with superior lethality to existing commercialized strains for managing this eucalyptus pest in Brazil.

scholarly journals Evaluating the virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) and Beauveria bassiana (Ascomycota: Hypocreales) isolates to Arabian rhinoceros beetle, Oryctes agamemnon arabicus

2015 ◽  
Vol 47 (3) ◽  
pp. 117 ◽  
Author(s):  
M.W. Khudhair ◽  
M.Z. Khalaf ◽  
H.F. Alrubeai ◽  
A.K. Shbar ◽  
B.S. Hamad ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Pupal Stage ◽  
Control Treatment ◽  
Rhinoceros Beetle ◽  
Oryctes Agamemnon ◽  
Lethal Time ◽  
Short Time

Virulence of entomopathogenic fungi <em>Metarhizium anisopliae</em> and <em>Beauveria bassiana</em> were tested against Arabian Rhinoceros Beetle, <em>Oryctes agamemnon arabicus</em> larvae. Four concentrations (1×10<sup>5</sup>, 1×10<sup>7</sup>, 1×10<sup>9</sup> and 1×10<sup>11</sup> conidia/mL<sup>–1</sup>) of two locally isolated entomopathogenic fungi spore suspensions were used in this study via larval direct spraying. Results revealed that both isolates can cause high mortality rate reaching 100% after 29 days. However, <em>Beauveria bassiana</em> scored higher mortality rate in short time especially at the concentration of 1×10<sup>11</sup> conidia/ mL<sup>–1</sup> with lethal time (LT)<sub>50</sub> 12.75 and LT<sub>90</sub> 20.00; while, <em>Metarhizium anisopliae</em> caused the higher percentage of malformed adults. Moreover, both isolates affected insect’s life cycle particularly in the pupal stage which was reduced remarkably by almost 50% in comparison with the control treatment.

scholarly journals Effect of Biofertilizers and Neem Oil on the Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

2001 ◽  
Vol 44 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Edson Hirose ◽  
Pedro M. O. J. Neves ◽  
João A. C. Zequi ◽  
Luís H. Martins ◽  
Cristiane H. Peralta ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Azadirachta Indica ◽  
Organic Agriculture ◽  
Metarhizium Anisopliae ◽  
Neem Oil ◽  
Colony Diameter ◽  
Negative Effect ◽  
Fungitoxic Effect

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion <FONT FACE=Symbol>Ô</FONT> and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibion<FONT FACE=Symbol>Ô</FONT> caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).

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