Pathogenicity of the Entomopathogenic Fungi Beauveria Bassiana ( Balsamo ) and Verticillium Lecanii ( Zimmerman ) against Aphid Macrosiphum Rosae , Linnaeus ( Hemiptera : Aphididae ) under Laboratory Conditions

2016 ◽  
Vol 9 (1) ◽  
pp. 25-28 ◽  
Author(s):  
Maryam Eidy ◽  
Hooshang Rafiee-Dastjerdi ◽  
Foroaddin Zargarzadeh
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Verticillium Lecanii ◽  
Laboratory Conditions ◽  
Macrosiphum Rosae

scholarly journals Presence of Toxic Substance in Fungus Bodies of the Entomopathogenic Fungi, Beauveria bassiana and Verticillium lecanii

1978 ◽  
Vol 13 (2) ◽  
pp. 97-102 ◽  
Author(s):  
Shigeo MURAKOSHI ◽  
Masakatsu ICHINOE ◽  
Akinori SUZUKI ◽  
Masaharu KANAOKA ◽  
Akira ISOGAI ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Toxic Substance ◽  
Verticillium Lecanii

scholarly journals Pathogenicity of three commercial products of entomopathogenic fungi, Beauveria bassiana, Metarhizum anisopilae and Lecanicillium lecanii against adults of olive fly, Bactrocera oleae (Gmelin) (Diptera: Tephritidae) in the laboratory

2009 ◽  
Vol 45 (No. 3) ◽  
pp. 98-102 ◽  
Author(s):  
M.F. Mahmoud
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Oral Treatment ◽  
Bactrocera Oleae ◽  
Commercial Products ◽  
Lecanicillium Lecanii ◽  
Laboratory Conditions ◽  
Olive Fly ◽  
Mortality Response ◽  
Lethal Time

The pathogenicity of entomopathogenic fungi, <I>Beauveria bassiana, Metarhizum anisopilae</I> and <I>Lecanicillium lecanii</I>, was evaluated against adults of the olive fly <I>Bactrocera oleae</I> (Gmelin) under laboratory conditions by two ways, contact bioassays and oral bioassays. The results showed that oral bioassays caused higher mortality after four treatments than the used contact bioassays. Moreover, the virulence of<I>L. lecanii</I> was higher than the virulence of <I>B. bassiana</I> and<I> M. anisopilae</I> in both ways of experiment. Lethal time (LT<sub>50</sub>) was shorter in oral bioassays than in contact bioassays in all treatments. It was 14.67, 8.30 and 5.43 days for <I>B. bassiana, M. anisopilae</I> and<I> L. lecanii</I> with oral treatment while it was 16.6, 26.07 and 12.59 days for <I>B. bassiana, M. anisopilae</I> and<I> L. lecanii</I>, respectively, with contact treatment. The slope values were 2.41, 2.55 and 2.37 for contact bioassays and 1.64, 1.69 and 1.61 for oral bioassays of <I>B. bassiana, M. anisopilae</I>and<I> L. lecanii</I>, respectively. The mortality response to the interaction between <I>B. bassiana</I> and <I>M. anisopilae</I> was synergistic while the interaction between <I>B. bassiana + L. lecanii</I> and <I>M. anisopilae + L. lecanii </I>showed an antagonistic response.

scholarly journals The influence of chitin synthesis inhibitors on growth of entomopathogenic fungi in vitro

2014 ◽  
Vol 27 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Anna Sapieha ◽  
Ryszard Miętkiewski
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Verticillium Lecanii ◽  
Chitin Synthesis ◽  
Chitin Synthesis Inhibitors ◽  
Paecilomyces Farinosus

<i>Beauveria bassiana, Paecilomyces farinosus, Verticillium lecanii, Conidiobolus thnmboides, Erynia pieris, E. radicans</i> were examined on medium to which were added three chitin synthesis inhibitors.

scholarly journals Compatibility and combined efficacy of entomopathogenic fungi and neonicotinoid insecticides against Myzus persicae (Sulzer): An ecofriendly approach

2021 ◽  
Vol 30 (1) ◽  
pp. 24
Author(s):  
Jaydeep Halder ◽  
Sujan Majumder ◽  
A. B. Rai
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Myzus Persicae ◽  
Metarhizium Anisopliae ◽  
Lethal Concentration ◽  
Lipaphis Erysimi ◽  
Verticillium Lecanii ◽  
Neonicotinoid Insecticides ◽  
Test Population ◽  
Lethal Time

Efficacy of different entomopathogenic fungi (EPF) viz., Beauveria bassiana, Metarhizium anisopliae and Lecanicillium (=Verticillium) lecanii and neonicotinoid insecticides i.e., Imidacloprid, Thiamethoxam and Acetamiprid were evaluated alone and their 1:1 combination against Lipaphis erysimi prevalent in vegetable ecosystem. Among the entomopathogenic fungi, B. bassiana was found most promising registering lowest median lethal time (LT50) of 48.17, 48.92 and 48.87 h during the period of 2018, 2019 and 2020, respectively, followed by L. lecanii (49.57, 49.45 and 50.46 h), M. anisopliae (51.81, 51.67 and 51.63 h). Amongst the three neonicotinoids, Acetamiprid was found more efficacious than the Imidacloprid and Thiamethoxam. Blending of B. bassiana and Acetamiprid at half of their recommended dose took lowest (22.76, 23.48 and 23.06 h during 2018, 2019 and 2020, respectively) lethal time to kill the fifty per cent test population followed by L. lecanii + Acetamiprid (22.58, 22.68, 22.52 h) and M. anisopliae + Acetamiprid (22.61, 23.82, 23.60 h). Combinations of these entomopathogenic fungi and neonicotinoid insecticides had co-toxicity co-efficient values > 1 and lower LT50 values than each of their individual indicating the compatibility amongst them. Co-application of these EPF with sub-lethal concentration of neonicotinoids could not only be a green ecofriendly option against this sucking pest but also able to minimize the chemical insecticides load in the environment.

scholarly journals Selecting native entomopathogenic fungi against Cosmopolites sordidus (Germar) in the laboratory

2021 ◽  
Vol 20 (1) ◽  
pp. 093-097
Author(s):  
Junior Steve Garrido Pérez ◽  
Richard Paredes-Espinosa ◽  
Giannfranco Egoávil Jump ◽  
Oniel Jeremias Aguirre Gil
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Mortality Rates ◽  
Paecilomyces Lilacinus ◽  
Cosmopolites Sordidus ◽  
Laboratory Conditions

The objective of this study was to select native isolates of entomopathogenic fungi against adult Cosmopolites sordidus in laboratory conditions. Four isolates of Beauveria bassiana, two isolates of Metarhizium anisopliae, and two isolates of Paecilomyces lilacinus were tested against C. sordidus. The entomopathogenic fungi that resulted in mortality rates over 50% were M. anisopliae (MA-CsCha and MA-Carabid) and B. bassiana (BB-CsCha and BB-CsLp). The most virulent isolates were MA-CsCha, BB-CsCha, and MA-Carabid with median lethal times (LT50) of 4.82, 5.4, and 5.79 days, respectively. In conclusion, the MA-CsCha, BB-CsCha, MA-Carabid, and BB-CsLp isolates are viable candidates to be tested in banana fields.

Effects of diphenols on the growth of three entomopathogenic fungi

1992 ◽  
Vol 38 (9) ◽  
pp. 1000-1003 ◽  
Author(s):  
Wen-Feng Hsiao ◽  
Michael J. Bidochka ◽  
George G. Khachatourians
Keyword(s):  
Key Words ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Fungal Growth ◽  
Defined Medium ◽  
Verticillium Lecanii ◽  
Insect Cuticle ◽  
Dihydroxyphenylacetic Acid ◽  
Yeast Extract Peptone Dextrose

The growth of three entomopathogenic fungi, Verticillium lecanii, Beauveria bassiana, and Metarhizium anisopliae, was determined in a yeast extract – peptone – dextrose (YPD) medium or a defined medium supplemented with dihydro-xyphenylalanine, dopamine, dopamine-HCl, norepinephrine-HCl, dihydroxyphenylacetic acid, or catechol. These diphenols may be found as part of the insect cuticle or hemolymph. Without diphenols YPD medium afforded generally higher growth of the fungi when compared with defined medium. Least growth was observed for B. bassiana and M. anisopliae in defined medium supplemented with dopamine, dopamine-HCl, or norepinephrine-HCl. Best growth for B. bassiana and M. anisopliae in defined medium was observed in unsupplemented medium or medium supplemented with dihydroxyphenylacetic acid, dihydroxyphenylalanine, or catechol. Best growth for V. lecanii was observed in a defined medium supplemented with dopamine, dopamine-HCl, or norepinephrine-HCl. In YPD medium, catechol inhibited growth of B. bassiana and M. anisopliae, whereas dihydroxyphenylalanine stimulated growth. The results showed similarities in M. anisopliae and B. bassiana response to various diphenols, whereas V. lecanii was different. Key words: diphenols, entomopathogenic fungi, Beauveria bassiana, Metarhizium anisopliae, Verticillium lecanii, fungal growth.

scholarly journals Effect of the native strain of the predator Nesidiocoris tenuis Reuter and the entomopathogenic fungi Beauveria bassiana and Lecanicillium muscarium against Bemisia tabaci (Genn.) under greenhouse conditions in Tunisia

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Besma Hamrouni Assadi ◽  
Sabrine Chouikhi ◽  
Refki Ettaib ◽  
Naima Boughalleb M’hamdi ◽  
Mohamed Sadok Belkadhi
Keyword(s):  
Beauveria Bassiana ◽  
Bemisia Tabaci ◽  
Entomopathogenic Fungi ◽  
Tobacco Plant ◽  
Economic Losses ◽  
Native Strain ◽  
Lecanicillium Muscarium ◽  
Southern Tunisia ◽  
Significant Efficacy ◽  
Nesidiocoris Tenuis

Abstract Background The misuse of chemical insecticides has developed the phenomenon of habituation in the whitefly Bemisia tabaci (Gennadius) causing enormous economic losses under geothermal greenhouses in southern Tunisia. Results In order to develop means of biological control appropriate to the conditions of southern Tunisia, the efficacy of the native strain of the predator Nesidiocoris tenuis Reuter (Hemiptera: Miridae) and two entomopathogenic fungi (EPF) Beauveria bassiana and Lecanicillium muscarium was tested against Bemisia tabaci (Gennadius). Indeed, the introduction of N. tenuis in doses of 1, 2, 3, or 4 nymphs per tobacco plant infested by the whitefly led to highly significant reduction in the population of B. tabaci, than the control devoid of predator. The efficacy of N. tenuis was very high against nymphs and adults of B. tabaci at all doses per plant with a rate of 98%. Likewise, B. bassiana and L. muscarium, compared to an untreated control, showed a very significant efficacy against larvae and adults of B. tabaci. In addition, the number of live nymphs of N. tenuis treated directly or introduced on nymphs of B. tabaci treated with the EPF remained relatively high, exceeding 24.8 nymphs per cage compared to the control (28.6). Conclusions It can be concluded that the native strain of N. tenuis and the EPF tested separately were effective against B. tabaci. Their combined use appears to be possible.

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