scholarly journals Cold tolerance of potato plants transformed with yeast invertase gene

2013 ◽  
Vol 57 (1-2) ◽  
pp. 31-39 ◽  
Author(s):  
Alexander N. Deryaabin ◽  
Irena M. Dubinina ◽  
Elena A. Burakhanova ◽  
Nina V. Astakhova ◽  
Elena P. Sabel´nikova ◽  
...  
Keyword(s):  
Cold Tolerance ◽  
Sugar Content ◽  
Invertase Activity ◽  
Peptide Sequence ◽  
Leader Peptide ◽  
Potato Plants ◽  
Patatin Promoter ◽  
Yeast Invertase ◽  
Yeast Invertase Gene ◽  
Invertase Gene

Our study was carried out with potato plants (<i>Solanum tuberosun</i> L.,cv. Désirée) transformed with the yeast invertase gene under the control of the B33 class I patatin promoter and with the proteinase inhibitor II leader peptide sequence providing for the apoplastic enzyme localization (B33-<i>inv</i> plants) and with the plants transformed with the reporter gene encoding bb-glucuronidase under the control of the 35S CaMV promoter (control plants). Exposure to 5°C during 6 days caused an increase in invertase activity and sugar content in B33-<i>inv</i> leaves in comparison with the control plants. Cell membranes of B33-<i>inv</i> plant cells showed greater cold tolerance under low temperature conditions than control plants that was recorded by electrolyte release. We supposed that higher cold tolerance of B33-<i>inv</i> plants was caused by stabilizing effect of sugar on the membranes, because B33-<i>inv</i> plants differ from the control plants in higher invertase activity, induced by expression of yeast invertase gene, and high content of sugars.

Morphology and tuber formation of in-vitro-grown potato plants harboring the yeast invertase gene and/or the rolC gene

1998 ◽  
Vol 18 (3-4) ◽  
pp. 318-324 ◽  
Author(s):  
G. A. Romanov ◽  
L. I. Sergeeva ◽  
S. A. Golyanovskaya ◽  
L. Willmitzer ◽  
T. Schm�lling ◽  
...  
Keyword(s):  
Tuber Formation ◽  
Rolc Gene ◽  
Potato Plants ◽  
Yeast Invertase ◽  
Yeast Invertase Gene ◽  
Invertase Gene

Effect of sugars on the development of oxidative stress induced by hypothermia in potato plants expressing yeast invertase gene

2007 ◽  
Vol 54 (1) ◽  
pp. 32-38 ◽  
Author(s):  
A. N. Deryabin ◽  
M. S. Sin’kevich ◽  
I. M. Dubinina ◽  
E. A. Burakhanova ◽  
T. I. Trunova
Keyword(s):  
Oxidative Stress ◽  
Potato Plants ◽  
Yeast Invertase ◽  
Yeast Invertase Gene ◽  
Invertase Gene

Influence of yeast-derived invertase gene expression in potato plants on membrane lipid peroxidation at low temperature

2005 ◽  
Vol 30 (1) ◽  
pp. 73-77 ◽  
Author(s):  
A.N. Deryabin ◽  
I.M. Dubinina ◽  
E.A. Burakhanova ◽  
N.V. Astakhova ◽  
E.P. Sabel’nikova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lipid Peroxidation ◽  
Low Temperature ◽  
Membrane Lipid ◽  
Membrane Lipid Peroxidation ◽  
Potato Plants ◽  
Invertase Gene

RELATIONSHIP BETWEEN ACID INVERTASE ACTIVITY AND SUGAR CONTENT IN GRAPE SPECIES

2011 ◽  
Vol 35 (6) ◽  
pp. 1646-1652 ◽  
Author(s):  
DEVAIAH KAMBIRANDA ◽  
HEMANTH VASANTHAIAH ◽  
SHEIKH M. BASHA
Keyword(s):  
Sugar Content ◽  
Invertase Activity ◽  
Acid Invertase

THE INVERTASE OF THE CORN RADICLE AND ITS ACTIVITY IN SUCCESSIVE STAGES OF GROWTH

1962 ◽  
Vol 40 (1) ◽  
pp. 113-126 ◽  
Author(s):  
J. A. Hellebust ◽  
D. F. Forward
Keyword(s):  
Reducing Sugars ◽  
Sugar Content ◽  
Kinetic Studies ◽  
Invertase Activity ◽  
Cell Number ◽  
Permeability Barrier ◽  
Ph Optimum ◽  
Intact Cells ◽  
Stages Of Growth ◽  
Growing Cell

Segments of the first 10 millimeters of corn radicle tips have been analyzed in terms of invertase activity, cell number, fresh and dry weights, and sugar content. Invertase activity per cell increased 40-fold as the meristematic cell advanced to the stage of most rapid elongation, and again subsided as the cell ceased to elongate and entered the stage of maturation. In the growing cell, the concentration of sucrose remained low while that of reducing sugars increased fivefold.The corn radicle invertase was found to be a β-fructofuranosidase with a Km of 0.006 M and a pH optimum of 4.6. Kinetic studies indicate that there is no change in the nature of the corn radicle invertase during cell growth. Equivalent activities of intact cells or segments and homogenates is consistent with the assumption that the enzyme is located outside the permeability barrier of the cells.

Roles played by invertase and gene expression in the development of the horn-shaped gall on leaves of Rhus chinensis

2017 ◽  
Vol 44 (12) ◽  
pp. 1160 ◽  
Author(s):  
Zhen-Yuan Ruan ◽  
Xiao-Ming Chen ◽  
Pu Yang ◽  
Bing-Yi Wang
Keyword(s):  
Cell Wall ◽  
Invertase Activity ◽  
Activity Level ◽  
Rapid Expansion ◽  
Sink Strength ◽  
Cell Wall Invertase ◽  
Vacuolar Invertase ◽  
Growing Period ◽  
Rhus Chinensis ◽  
Invertase Gene

The present study deals with the growth and development of the horn-shaped gall, which is induced by Schlechtendalia chinensis Bell. on leaves of Rhus chinensis Mill. The relationship between gall formers and their host plants was investigated by means of the activities of various invertases, the expressions of the cell wall invertase gene (INV2), and vacuolar invertase gene (INV3) during gall development. Our results show that the increase in the sink strength of the galls required cell wall invertase and vacuolar invertase, and that vacuolar invertase had a particular impact during the early development. In addition, vacuolar invertase activity was always significantly higher in galls than in leaves. However, ionically bound cell wall invertase showed a slightly significant increased activity level when compared with the leaves after galls had entered the fast growing period. This result indicates that vacuolar invertase is related to the rapid expansion of the galls, but ionically bound cell wall invertase is involved in the rapid growth of tissues. The enhanced activity of cell wall invertase and the expression of INV2 may be a plant response to a gall-induced stress. Cytoplasmic invertase that acts as a maintenance enzyme, or takes part in the production of secondary metabolites, was elevated when intracellular acid invertase activity decreased.

Metabolic engineering of invertase activities in different subcellular compartments affects sucrose accumulation in sugarcane cells

2000 ◽  
Vol 27 (11) ◽  
pp. 1021 ◽  
Author(s):  
Hongmei Ma ◽  
Henrik H. Albert ◽  
Robert Paull ◽  
Paul H. Moore
Keyword(s):  
Fold Increase ◽  
Saccharum Officinarum ◽  
Invertase Activity ◽  
Acid Invertase ◽  
Sucrose Accumulation ◽  
Sugar Composition ◽  
Transgenic Sugarcane ◽  
Soluble Acid Invertase ◽  
Invertase Gene ◽  
Soluble Acid

Transgenic sugarcane (Saccharum officinarum L.) lines were created to express altered invertase isoform activity to elucidate the role(s) of invertase in the sucrose accumulation process. A sugarcane soluble acid invertase cDNA (SCINVm, AF062734) in the antisense orientation was used to decrease invertase activity. The Saccharomyces cerevisiae invertase gene (SUC2), fused with appropriate targeting elements, was used to increase invertase activity in the apoplast, cytoplasm and vacuole. A callus/liquid culture system was established to evaluate change in invertase activity and sugar concentration in the transgenic lines. Increased invertase activity in the apoplast led to rapid hydrolysis of sucrose and rapid increase of hexose in the medium. The cellular hexose content increased dramatically and the sucrose level decreased. Cells with higher cytoplasmic invertase activity did not show a significant change in the sugar composition in the medium, but did significantly reduce the sucrose content in the cells. Transformation with the sugarcane antisense acid invertase gene produced a cell line with moderate inhibition of soluble acid invertase activity and a 2-fold increase in sucrose accumulation. Overall, intracellular and extracellular sugar composition was very sensitive to the change in invertase activities. Lowering acid invertase activity increased sucrose accumulation.

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