Metabolic engineering of invertase activities in different subcellular compartments affects sucrose accumulation in sugarcane cells

2000 ◽  
Vol 27 (11) ◽  
pp. 1021 ◽  
Author(s):  
Hongmei Ma ◽  
Henrik H. Albert ◽  
Robert Paull ◽  
Paul H. Moore
Keyword(s):  
Fold Increase ◽  
Saccharum Officinarum ◽  
Invertase Activity ◽  
Acid Invertase ◽  
Sucrose Accumulation ◽  
Sugar Composition ◽  
Transgenic Sugarcane ◽  
Soluble Acid Invertase ◽  
Invertase Gene ◽  
Soluble Acid

Transgenic sugarcane (Saccharum officinarum L.) lines were created to express altered invertase isoform activity to elucidate the role(s) of invertase in the sucrose accumulation process. A sugarcane soluble acid invertase cDNA (SCINVm, AF062734) in the antisense orientation was used to decrease invertase activity. The Saccharomyces cerevisiae invertase gene (SUC2), fused with appropriate targeting elements, was used to increase invertase activity in the apoplast, cytoplasm and vacuole. A callus/liquid culture system was established to evaluate change in invertase activity and sugar concentration in the transgenic lines. Increased invertase activity in the apoplast led to rapid hydrolysis of sucrose and rapid increase of hexose in the medium. The cellular hexose content increased dramatically and the sucrose level decreased. Cells with higher cytoplasmic invertase activity did not show a significant change in the sugar composition in the medium, but did significantly reduce the sucrose content in the cells. Transformation with the sugarcane antisense acid invertase gene produced a cell line with moderate inhibition of soluble acid invertase activity and a 2-fold increase in sucrose accumulation. Overall, intracellular and extracellular sugar composition was very sensitive to the change in invertase activities. Lowering acid invertase activity increased sucrose accumulation.

scholarly journals Levels and Role of Sucrose Synthase, Sucrose-phosphate Synthase, and Acid Invertase in Sucrose Accumulation in Fruit of Asian Pear

1992 ◽  
Vol 117 (2) ◽  
pp. 274-278 ◽  
Author(s):  
Takaya Moriguchi ◽  
Kazuyuki Abe ◽  
Tetsuro Sanada ◽  
Shohei Yamaki
Keyword(s):  
Sucrose Synthase ◽  
Sucrose Phosphate Synthase ◽  
Invertase Activity ◽  
Acid Invertase ◽  
Sucrose Accumulation ◽  
Sucrose Content ◽  
Soluble Acid Invertase ◽  
Asian Pear ◽  
Soluble Acid ◽  
Sucrose Phosphate

Soluble sugar content and activities of the sucrose-metabolizing enzymes sucrose synthase (SS) (EC 2.4.1.13), sucrose-phosphate synthase (SPS) (EC 2.4.1.14), and acid invertase (EC 2.4.1.26) were analyzed in the pericarp of fruit from pear cultivars that differed in their potential to accumulate sucrose to identify key enzymes involved in sucrose accumulation in Asian pears. The Japanese pear `Chojuro' [Pyrus pyrifolia (Burro. f.) Nakai] was characterized as a high-sucrose-accumulating type based on the analysis of mature fruit, while the Chinese pear `Yali' (P. bretschneideri Rehd.) was a low-sucrose-accumulating type throughout all developmental stages. The activity of SS and SPS in `Chojuro' increased during maturation concomitant with sucrose accumulation, whereas the activity of these enzymes in `Yali' did not increase during maturation. The activity of SS and SPS in the former were seven and four times, respectively, higher than those in the latter at the mature stage. Further, among 23 pear cultivars, SS activity was closely correlated with sucrose content, while SPS activity was weakly correlated. Soluble acid invertase activity in `Chojuro' and `Yali' decreased with fruit maturation, but the relationships between soluble invertase activity and sucrose content were not significant. The results indicate that SS and SPS are important determinants of sucrose accumulation in Asian pear fruit and that a decrease of soluble acid invertase activity is not absolutely required for sucrose accumulation.

Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane

2011 ◽  
Vol 33 (5) ◽  
pp. 1749-1757 ◽  
Author(s):  
Ashok K. Verma ◽  
Santosh K. Upadhyay ◽  
Manoj K. Srivastava ◽  
Praveen C. Verma ◽  
S. Solomon ◽  
...  
Keyword(s):  
Invertase Activity ◽  
Acid Invertase ◽  
Transcript Expression ◽  
Sucrose Accumulation ◽  
Soluble Acid Invertase ◽  
Soluble Acid

Change of soluble acid invertase gene (SAI-1) haplotype in hybrid sorghum breeding program in China

2021 ◽  
Vol 41 (6) ◽  
Author(s):  
Hai-Li Zhong ◽  
Yang Liu ◽  
Yuan-Dong Nie ◽  
Zhi Wang ◽  
Li Zhu ◽  
...  
Keyword(s):  
Acid Invertase ◽  
Breeding Program ◽  
Soluble Acid Invertase ◽  
Sorghum Breeding ◽  
Invertase Gene ◽  
Soluble Acid

scholarly journals Plant regulators and invertase activity in sugarcane at the beginning of the harvest season

2015 ◽  
Vol 45 (10) ◽  
pp. 1788-1794 ◽  
Author(s):  
Glauber Henrique Pereira Leite ◽  
Carlos Alexandre ◽  
Costa Crusciol ◽  
Gabriela Ferraz de Siqueira ◽  
Marcelo de Almeida Silva
Keyword(s):  
Invertase Activity ◽  
Acid Invertase ◽  
Active Principle ◽  
Sugarcane Variety ◽  
Growth Inhibitors ◽  
Soluble Acid Invertase ◽  
Neutral Invertase ◽  
Soluble Acid ◽  
In The Beginning ◽  
High Sucrose

Invertases play an essential role in partitioning photosynthates between storage and growth. The objective of this study was to evaluate the activity of acid and neutral invertases and the role they play in controlling the accumulation of sucrose in sugarcane as a result of the application of plant regulators in the beginning of the cropping season.A randomized block experimental design was adopted, with five replicates.The treatments consisted in the application of three plant regulators of the class of growth inhibitors (Sulfomethuron-methyl - 20g ha-1, Glyphosate - 0.4L ha-1, and Compounds from organic carboxylic radicals + Glyphosate - 1L ha-1 + 0.15L ha-1), in addition to a control (natural ripening).The acid and neutral invertase levels are affected in different ways and intensities, due to the active principle used as ripening agent and to the weather conditions.In sugarcane variety RB85-5453, with the conditions described in this experiment, it is suggested high levels of soluble acid invertase in relation to levels of neutral invertase; however, the first was characterized by high sucrose content in the stalks.Inverse correlation could be established for sugarcane variety RB85-5453 between soluble acid invertase levels and effective sucrose accumulation in the stalks.

Soluble Acid Invertase (SAI) Activity and Gene Expression Controlling Sugar Composition in Sugarcane

Sugar Tech ◽  
2017 ◽  
Vol 19 (6) ◽  
pp. 669-674 ◽  
Author(s):  
Radha Jain ◽  
S. P. Singh ◽  
Anshu Singh ◽  
Smita Singh ◽  
Ram Kishor ◽  
...  
Keyword(s):  
Gene Expression ◽  
Acid Invertase ◽  
Sugar Composition ◽  
Soluble Acid Invertase ◽  
Soluble Acid

Physiological basis for enhanced sucrose accumulation in an engineered sugarcane cell line

2010 ◽  
Vol 37 (12) ◽  
pp. 1161 ◽  
Author(s):  
Luguang Wu ◽  
Robert G. Birch
Keyword(s):  
Sucrose Synthase ◽  
Sucrose Phosphate Synthase ◽  
Saccharum Officinarum ◽  
Invertase Activity ◽  
Negative Regulator ◽  
Sucrose Accumulation ◽  
Physiological Basis ◽  
Transgenic Sugarcane ◽  
Extracellular Invertase ◽  
Sucrose Phosphate

Transgenic sugarcane (Saccharum officinarum L. interspecific hybrids) line N3.2 engineered to express a vacuole-targeted sucrose isomerase was found to accumulate sucrose to twice the level of the background genotype Q117 in heterotrophic cell cultures, without adverse effects on cell growth. Isomaltulose levels declined over successive subcultures, but the enhanced sucrose accumulation was stable. Detailed physiological characterisation revealed multiple processes altered in line N3.2 in a direction consistent with enhanced sucrose accumulation. Striking differences from the Q117 control included reduced extracellular invertase activity, slower extracellular sucrose depletion, lower activities of symplastic sucrose-cleavage enzymes (particularly sucrose synthase breakage activity), and enhanced levels of symplastic hexose-6-phosphate and trehalose-6-phosphate (T6P) in advance of enhanced sucrose accumulation. Sucrose biosynthesis by sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP) was substantially faster in assays conducted to reflect the elevation in key allosteric metabolite glucose-6-phosphate (G6P). Sucrose-non-fermenting-1-related protein kinase 1 (SnRK1, which typically activates sucrose synthase breakage activity while downregulating SPS in plants) was significantly lower in line N3.2 during the period of fastest sucrose accumulation. For the first time, T6P is also shown to be a negative regulator of SnRK1 activity from sugarcane sink cells, hinting at a control circuitry for parallel activation of key enzymes for enhanced sucrose accumulation in sugarcane.

scholarly journals 746 PB 102 DIFFERENCES IN SUCROSE METABOLISM RELATIVE TO ACCUMULATION OF BIRD-DETERRENT SUCROSE LEVELS IN FRUIT OF VACCINIUM SPECIES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 540a-540
Author(s):  
R.L. Darnell ◽  
R. Cano-Medrano ◽  
K.E. Koch
Keyword(s):  
Sucrose Concentration ◽  
Sucrose Phosphate Synthase ◽  
Invertase Activity ◽  
Sucrose Accumulation ◽  
Ripe Fruit ◽  
Soluble Acid Invertase ◽  
Apparent Correlation ◽  
Soluble Acid ◽  
High Sucrose ◽  
Sucrose Phosphate

Variability in sucrose levels and metabolism in ripe fruit of several Vaccinium species were examined. The objective was to determine if sufficient variability for fruit sucrose accumulation was present in existing populations to warrant attempts to breed for high-sucrose fruit, which potentially would be less subject to bird predation. Three-fold differences in fruit sucrose concentration were found among species, ranging from 19 to 24 mg·(g fw)-1 in V. stamineum and V. arboreum to about 7 mg·(g fw)-1 in cultivated blueberry (V. ashei and V. corymbosum) and V. darrowi. Soluble acid invertase activity was negatively correlated with fruit sucrose concentration. There was no apparent correlation between fruit sugar concentration and either sucrose phosphate synthase or sucrose synthase activities, both of which were low for all species studied. The degree of variability in fruit sucrose accumulation among Vaccinium species supports the feasibility of developing high sucrose fruit, which would be a potentially valuable addition to current strategies of minimizing crop losses to birds.

Sign in / Sign up

Export Citation Format

Share Document