Morphology and tuber formation of in-vitro-grown potato plants harboring the yeast invertase gene and/or the rolC gene

1998 ◽  
Vol 18 (3-4) ◽  
pp. 318-324 ◽  
Author(s):  
G. A. Romanov ◽  
L. I. Sergeeva ◽  
S. A. Golyanovskaya ◽  
L. Willmitzer ◽  
T. Schm�lling ◽  
...  
Keyword(s):  
Tuber Formation ◽  
Rolc Gene ◽  
Potato Plants ◽  
Yeast Invertase ◽  
Yeast Invertase Gene ◽  
Invertase Gene

scholarly journals Cold tolerance of potato plants transformed with yeast invertase gene

2013 ◽  
Vol 57 (1-2) ◽  
pp. 31-39 ◽  
Author(s):  
Alexander N. Deryaabin ◽  
Irena M. Dubinina ◽  
Elena A. Burakhanova ◽  
Nina V. Astakhova ◽  
Elena P. Sabel´nikova ◽  
...  
Keyword(s):  
Cold Tolerance ◽  
Sugar Content ◽  
Invertase Activity ◽  
Peptide Sequence ◽  
Leader Peptide ◽  
Potato Plants ◽  
Patatin Promoter ◽  
Yeast Invertase ◽  
Yeast Invertase Gene ◽  
Invertase Gene

Our study was carried out with potato plants (<i>Solanum tuberosun</i> L.,cv. Désirée) transformed with the yeast invertase gene under the control of the B33 class I patatin promoter and with the proteinase inhibitor II leader peptide sequence providing for the apoplastic enzyme localization (B33-<i>inv</i> plants) and with the plants transformed with the reporter gene encoding bb-glucuronidase under the control of the 35S CaMV promoter (control plants). Exposure to 5°C during 6 days caused an increase in invertase activity and sugar content in B33-<i>inv</i> leaves in comparison with the control plants. Cell membranes of B33-<i>inv</i> plant cells showed greater cold tolerance under low temperature conditions than control plants that was recorded by electrolyte release. We supposed that higher cold tolerance of B33-<i>inv</i> plants was caused by stabilizing effect of sugar on the membranes, because B33-<i>inv</i> plants differ from the control plants in higher invertase activity, induced by expression of yeast invertase gene, and high content of sugars.

Effect of sugars on the development of oxidative stress induced by hypothermia in potato plants expressing yeast invertase gene

2007 ◽  
Vol 54 (1) ◽  
pp. 32-38 ◽  
Author(s):  
A. N. Deryabin ◽  
M. S. Sin’kevich ◽  
I. M. Dubinina ◽  
E. A. Burakhanova ◽  
T. I. Trunova
Keyword(s):  
Oxidative Stress ◽  
Potato Plants ◽  
Yeast Invertase ◽  
Yeast Invertase Gene ◽  
Invertase Gene

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu
Keyword(s):  
Solanum Tuberosum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Leaf Extract ◽  
Solanum Tuberosum L ◽  
In Vitro Toxicity ◽  
Potato Plants ◽  
Colony Diameter ◽  
In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

scholarly journals Expression Level of Mature miR172 in Wild Type and StSUT4-Silenced Plants of Solanum tuberosum Is Sucrose-Dependent

2021 ◽  
Vol 22 (3) ◽  
pp. 1455
Author(s):  
Varsha Garg ◽  
Aleksandra Hackel ◽  
Christina Kühn
Keyword(s):  
Solanum Tuberosum ◽  
Wild Type ◽  
Potato Plants ◽  
Mature Leaves ◽  
In Vitro Plantlets ◽  
Long Time ◽  
Short Time ◽  
Cut Leaves

In potato plants, the phloem-mobile miR172 is involved in the sugar-dependent transmission of flower and tuber inducing signal transduction pathways and a clear link between solute transport and the induction of flowering and tuberization was demonstrated. The sucrose transporter StSUT4 seems to play an important role in the photoperiod-dependent triggering of both developmental processes, flowering and tuberization, and the phenotype of StSUT4-inhibited potato plants is reminiscent to miR172 overexpressing plants. The first aim of this study was the determination of the level of miR172 in sink and source leaves of StSUT4-silenced as well as StSUT4-overexpressing plants in comparison to Solanum tuberosum ssp. Andigena wild type plants. The second aim was to investigate the effect of sugars on the level of miRNA172 in whole cut leaves, as well as in whole in vitro plantlets that were supplemented with exogenous sugars. Experiments clearly show a sucrose-dependent induction of the level of mature miR172 in short time as well as long time experiments. A sucrose-dependent accumulation of miR172 was also measured in mature leaves of StSUT4-silenced plants where sucrose export is delayed and sucrose accumulates at the end of the light period.

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