scholarly journals Efficacy of Pseudomonas aeruginosa and other biocontrol agents in the control of root rot infection in cotton

2013 ◽  
Vol 50 (1-2) ◽  
pp. 5-10 ◽  
Author(s):  
Shamama Shamim ◽  
Nuzhat Ahmad ◽  
Atta Rahman ◽  
S. Ehteshmul-Haque ◽  
Abdul Ghaffar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Rhizoctonia Solani ◽  
Plant Height ◽  
Fusarium Solani ◽  
Root Rot ◽  
Macrophomina Phaseolina ◽  
Fresh Weight ◽  
Trichoderma Koningii ◽  
Pot Experiments ◽  
Combined Use

Seed dressing with <em>Pseudomonas aeruginosa, Paecilomyces lilacinus and Trichoderma koningii</em> significantly (p<0,05) reduced infection of <em>Macrophomina phaseolina, Rhizoctonia solani</em> and <em>Fusarium solani</em> on cotton roots in pot experiments and in field. Combined use of P.aeruginosa strain CMG63 with <em>T.koningii</em> produced greater plant height and fresh weight of shoot in field as compared to CMG52 which showed better results in pot experiments.

scholarly journals Interaction of Fusarium solani, Macrophomina phaseolina and Rhizoctonia solani as root rot pathogens of Cucumis melo

2019 ◽  
Vol 45 (4) ◽  
pp. 355-360
Author(s):  
Maria Alice Formiga Porto ◽  
Márcia Michelle de Queiroz Ambrósio ◽  
Selma Rogéria de Carvalho Nascimento ◽  
Beatriz Letícia Silva da Cruz ◽  
Taffarel Melo Torres
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Solani ◽  
Root Rot ◽  
Cucumis Melo ◽  
Yield Loss ◽  
Disease Incidence ◽  
Macrophomina Phaseolina ◽  
Fresh Weight ◽  
Root Diseases ◽  
Mixed Inoculum

ABSTRACT Root diseases represent one of the main reasons for yield loss in melon crops, especially root and stem rots caused by pathogens like the fungi Fusarium solani (Fs), Macrophomina phaseolina (Mp) and Rhizoctonia solani (Rs), frequently observed in muskmelon either alone or in combination. The objective of this study was to evaluate the effect of the interaction between the pathogens Fs, Mp and Rs on the incidence and severity of root rot and muskmelon development. Two greenhouse experiments were performed using plastic pots with substrate infested with each pathogenic agent alone or in combination. The second experiment was conducted in the same pots that were used in the first experiment. In the first experiment, the disease incidence was higher for the treatment with Fs alone. In the second experiment, the disease incidence and severity were greater for treatment Fs + Rs than for Fs alone. Macrophomina phaseolina was the most commonly isolated pathogen when applied to the plants in a paired mixed inoculum (Fs + Mp and Mp + Rs) in the first experiment. In the second experiment, Fs was more prevalent than the other studied pathogens. Soil infested with Fs had the lowest fresh weight of muskmelon. The pathogens Fs and Mp were more competitive than Rs.

scholarly journals Effect of crustacean chitin on the efficacy of plant growth promoting bacteria in the control of root infecting fungi of sunflower and chickpea

2013 ◽  
Vol 53 (1) ◽  
pp. 5-12 ◽  
Author(s):  
Viqar Sutana ◽  
Syed Ehteshamul-Haque ◽  
Jehan Ara ◽  
Rashid Qasim ◽  
Abdul Ghaffar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Plant Growth ◽  
Fusarium Solani ◽  
Soil Amendment ◽  
Macrophomina Phaseolina ◽  
Plant Growth Promoting Bacteria ◽  
Fresh Weight ◽  
Plant Growth Promoting ◽  
Growth Promoting

Soil amendment with crustacean chitin used alone or with <em>Pseudomonas aeruginosa Bacillus subtilis</em> significantly (p<0,05) reduced infection of <em>Rhizoctonia solani Fusarium solani</em> on sunflower and <em>R.solani</em> on chickpea. Crab chitin used alone or with <em>P.aeruginosa</em> or <em>B.subtilis</em> completely controlled the infection of <em>Macrophomina phaseolina</em> on chickpea. Prawn or shrimp powder used 1% w/w of soil was found phytotoxic on chickpea but not on sunflower. Maximum fresh weight of shoot was produced by <em>P.aeruginosa</em> used with shrimp powder in sunflower and with crab chitin in chickpea. <em>P.aeruginosa</em> produced greater plant height in chickpea used with shrimp chitin.

scholarly journals Control of the root-rot and root-knot disease complex by Pseudomonas aeruginosa: impact of bacterial rhizosphere colonization

2013 ◽  
Vol 54 (1) ◽  
pp. 55-64
Author(s):  
I. A. Siddiqui ◽  
S. Ehteshamul-Haque ◽  
S. S.. Shaukat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fusarium Solani ◽  
Root Rot ◽  
Meloidogyne Javanica ◽  
Macrophomina Phaseolina ◽  
Root Infection ◽  
Gall Formation ◽  
Root Knot Nematode ◽  
Rhizosphere Colonization ◽  
Growth Promoting

The potential of 3 <i>Pseudomonas aeriuginosa</i> strains as biocontrol agents of rootinfecting fungi <i>Macrophomina phaseolina, Fusarium solani</i> and <i>Rhizoctonia solani</i> and the root-knot nematode <i>Meloidogyne javanica</i> was tested on chili and uridbean under greenhouse conditions. All the three strains significantly reduced nematode populations in soil, invasion, multiplication and gall formation due to <i>M.javanica</i>. Root infection by fungi was also effectively suppressed following <i>P.aeruginosa</i> application. Bacterial antagonists exhibited better biocontrol and growth promoting activity in 15-day-old plants than did those harvested at 30 or 45 days. Population of the bacterium in the rhizosphere declined rapidly after 15 days of nematode inoculation. Strain Pa-5 showed maximum nodulation in 15-day-old samplings while strain Pa-7 showed highest number of nodules in 30 and 45-day-old uridbean plants.

scholarly journals In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Alemayehu Dugassa ◽  
Tesfaye Alemu ◽  
Yitbarek Woldehawariat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Fluorescens ◽  
Faba Bean ◽  
Fusarium Solani ◽  
Root Rot ◽  
Mycelial Growth ◽  
Biocontrol Agents ◽  
Black Root Rot ◽  
Root Rot Disease ◽  
Rot Disease

Abstract Background Faba bean (Vicia faba L.) cultivation is highly challenged by faba bean black root rot disease (Fusarium solani) in high lands of Ethiopia. To ensure sustainable production of faba beans, searching for eco-friendly disease management options is necessary to curb the progress of the disease timely. The indigenous biocontrol agents that suit local environments may effectively strive with in-situ microorganisms and suppress local pathogen strains. This study aimed to screen antagonistic indigenous compatible Trichoderma and Pseudomonas strains against Fusarium solani. In the pathogenicity test, soil-filled pots were arranged in complete random block design and sown with health faba bean seeds. The effect of some fungicides was evaluated against Fusarium by food poisoning methods to compare with the biocontrol agents. The antagonistic efficacy of biocontrol agents and their compatibility was investigated on Potato dextrose agar medium. Results Fusarium solani AAUF51 strain caused an intense root rotting in faba bean plant. The effect of Mancozeb 80% WP at 300 ppm was comparable with Trichoderma and Pseudomonas strains against Fusarium. The mycelial growth of test the pathogen was significantly (P ≤ 0.05) reduced to 86.67 and 85.19% by Trichoderma harzianum AAUW1 and Trichoderma viridae AAUC22 strains in dual culture, respectively. The volatile metabolites of Pseudomonas aeruginosa AAUS31 (77.78%) found the most efficient in reducing mycelial growth of Fusarium followed by Pseudomonas fluorescens AAUPF62 (71.11%) strains. The cell-free culture filtrates of Pseudomonas fluorescens AAUPF62 and Pseudomonas aeruginosa AAUS31 were more efficient than the Trichoderma strain in reducing the growth of Fusarium isolates. There was no zone of inhibition recorded between Trichoderma harzianum AAUW1, Trichoderma viridae AAUC22, Pseudomonas aeruginosa AAUS31, and Pseudomonas fluorescens AAUPF62 strains, hence they were mutually compatible. Conclusions The compatible Trichoderma and Pseudomonas strains showed antagonistic potentiality that could be explored for faba bean protection against black root rot disease and might have a future dual application as biocontrol agents.

Root-rot resistance in common bean

1993 ◽  
Vol 73 (1) ◽  
pp. 365-367 ◽  
Author(s):  
J. C. Tu ◽  
S. J. Park
Keyword(s):  
Phaseolus Vulgaris ◽  
Rhizoctonia Solani ◽  
Common Bean ◽  
Fusarium Oxysporum ◽  
Key Words ◽  
Fusarium Solani ◽  
Root Rot ◽  
Pythium Ultimum ◽  
Growth Room

A bean (Phaseolus vulgaris) line, A - 300, resistant to Rhizoctonia solani and Fusarium oxysporum was introduced into Ontario from Colombia. The results of tests conducted in a root-rot nursery, in a greenhouse and in a growth room showed that this bean line is resistant to Fusarium solani f. sp. phaseoli and Pythium ultimum. Key words: Bean, Phaseolus vulgaris, root rot resistance

Pathogenic effects of Heterodera avenae and Rhizoctonia solani and their interaction on wheat

1971 ◽  
Vol 22 (2) ◽  
pp. 189 ◽  
Author(s):  
JW Meagher ◽  
SC Chambers
Keyword(s):  
Rhizoctonia Solani ◽  
Plant Height ◽  
Root Length ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Heterodera Avenae ◽  
Root Number ◽  
Fresh Weight ◽  
Wheat Seedlings ◽  
Pathogenic Effects

The effects of the cereal cyst nematode Heterodera arenae and the fungus Rhizoctonin solani on the growth of wheat seedlings were examined under glasshouse conditions. In combination, the pathogens caused significantly greater reduction in tillering, plant height, fresh weight, and root number and length than that caused by either pathogen when acting alone. Reduction in plant height, root number, and root length were the result of interaction. Nematode-fungus interaction may be the cause of disease in the field usually attributed to only one of these pathogens.

scholarly journals Antagonismo de Trichoderma harzianum contra la fusariosis del garbanzo y su efecto biofertilizante

2020 ◽  
Vol 11 (5) ◽  
pp. 1135-1147
Author(s):  
Talina Olivia Martínez-Martínez ◽  
Brenda Zulema Guerrero-Aguilar ◽  
Víctor Pecina-Quintero ◽  
Enrique González-Pérez ◽  
Juan Gabriel Angeles-Núñez
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Fusarium Solani ◽  
Baja California ◽  
Sclerotium Rolfsii ◽  
Macrophomina Phaseolina

El garbanzo es una leguminosa, que se cultiva en dos regiones de México principalmente, noroeste (Sonora, Sinaloa y Baja california) y la región de El Bajío (Guanajuato, Michoacán y Jalisco); sin embargo, cada año la producción del cultivo está comprometida con la fusariosis vascular, una de las principales enfermedades que afectan al cultivo y que está asociada al complejo fúngico Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Macrophomina phaseolina y Sclerotium rolfsii. Una alternativa de control biológico es la aplicación de Trichoderma, la que además tiene un efecto indirecto en la nutrición de la planta. El objetivo de este estudio fue determinar el antagonismo in vitro de dos cepas de Trichoderma harzianum (T1 y T2) y su efecto como biofertilizante. Se realizaron confrontaciones in vitro contra cepas de las razas de Fusariumoxysporum f. sp. ciceris (Foc 0, 1B/C, 5 y 6), Fusarium solani, Macrophomina phaseolina (MSonora y M-GTO) y Sclerotium rolfsii. Se evaluó el efecto de T2 como biofertilizante (TB) midiendo las variables: número de flores, vainas, altura de la planta, diámetro del tallo, longitud de la raíz y rendimiento de grano. Las dos cepas de T. harzianum mostraron antagonismo en diferente escala contra los patógenos. Adicionalmente, con el tratamiento donde se aplicó T. harzianum (TB) se presentaron incrementos en el número de flores (30%), vainas (24%), altura (3%), diámetro de las plantas (3.5%), así como la longitud de la raíz (13%) y rendimiento del grano (23%).

scholarly journals Soil amendment with Aerva javanica (Burm. F.) Juss. ex Schult. in the control of root rot fungi of cowpea [Vigna unguiculata (L.) Walp.] and mung bean [Vigna radiata (L.)]

2012 ◽  
Vol 65 (3) ◽  
pp. 69-74 ◽  
Author(s):  
Naheed Ikram ◽  
Shahnaz Dawar
Keyword(s):  
Vigna Unguiculata ◽  
Root Rot ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Growth Parameters ◽  
Macrophomina Phaseolina ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Fresh Weight ◽  
Plant Parts

Root rot fungi cause severe losses of crop plants, so the present work was carried out to determine the effect of <em>Aerva javanica </em>parts powder on root infecting fungi of mung bean (<em>Vigna radiata </em>(L.) and cowpea (<em>Vigna unguiculata </em>(L.) Walp.). <em>A. javanica </em>parts (stem, leaves and flower) were used as soil amendments at 0.1, 1 and 5% to check the effectiveness on growth parameters. All the plant parts showed a significant reduction in root rot fungi like <em>Fusarium </em>spp., <em>Rhizoctonia solani </em>Kuhn, and <em>Macrophomina phaseolina </em>(Tassi) Goid. It was noted that germination percentage, fresh weight, leaf area and number of nodules were significantly higher and the inhibitory effect on root rot fungi increased when the soil was amended with <em>A. javanica </em>leaves at 1%. Thus, among all the treatments, <em>A. javanica </em>leaves at 1% were found to be the most effective against root rot fungi.

In-vitro bioefficacy of fungal antagonists against root rot of Pea caused by Rhizoctonia solani (Kuhn)

2018 ◽  
Vol 3 (02) ◽  
pp. 166-170
Author(s):  
Safdar Kaiser Hasmi ◽  
R. U. Khan
Keyword(s):  
Growth Inhibition ◽  
Rhizoctonia Solani ◽  
Root Rot ◽  
Radial Growth ◽  
Culture Technique ◽  
Dual Culture ◽  
Time Control ◽  
Trichoderma Koningii ◽  
Petri Plate

In-vitro effectiveness of various antagonistic fungi namely Aspergillus niger, A. flavus, Trichoderma koningii, T. atroviride, and T. harzianum were evaluated against Rhizoctonia solani by dual culture technique on potato dextrose agar. According to the observation recorded after 5 days, all the treatments were found to be superior over control (R. solani), but among all treatments A. niger was found to the most effective antagonist, with highest radial growth inhibition of the pathogen (77.01 percent), followed by A. flavus, T. harzianum and T. koningii i.e., 66.23, 64.42 and 62.20 percent. While as, T. atroviride was found to be the least effective one with minimum growth inhibition i.e., 42.21 percent. Whereas, at the same time control (R. solani) showed 100 percent radial growth and covered the whole Petri Plate within 5 days. All the bio-control agents were significantly effective to inhibit the sclerotia formation and development, except T. atroviride in which formation of sclerotia was recorded, while in all other treatments complete inhibition of sclerotia formation was recorded after 10 days of incubation.

scholarly journals Feijão-de-porco (Canavalia ensiformis) no controle da podridão radicular do meloeiro causada por associação de patógenos

2016 ◽  
Vol 42 (4) ◽  
pp. 327-332
Author(s):  
Maria Alice Formiga Porto ◽  
◽  
Márcia Michelle de Queiroz Ambrósio ◽  
Francisco Cláudio Lopes de Freitas ◽  
Selma Rogéria de Carvalho Nascimento ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Solani ◽  
Cucumis Melo ◽  
Macrophomina Phaseolina ◽  
Canavalia Ensiformis ◽  
Cucumis Melo L

RESUMO A ocorrência de doenças radiculares representa uma das principais causas de perda de rendimento na cultura do melão, com destaque para patógenos causadores das podridões de raízes e colos, como os fungos Fusarium solani, Macrophomina phaseolina e Rhizoctonia solani, observados no meloeiro tanto de forma isolada quanto associada. A utilização de material vegetal e restos de culturas, além de melhorar as propriedades físicas do solo e favorecer a atividade microbiana neste ambiente, pode interferir negativamente sobre a população de patógenos. Portanto, o objetivo deste trabalho foi verificar o efeito da utilização do Feijão-de-porco [Canavalia ensiformis (L.) DC] no manejo da podridão radicular do meloeiro (Cucumis melo L.) causada pelos patógenos F. solani, M. phaseolina e R. solani, quando sozinhos ou associados. O experimento foi realizado em casa de vegetação no delineamento de blocos casualizados, com esquema fatorial 8 x 4, sendo 8 tipos de fungos/associações e 4 formas de manejo, com 4 repetições. As características avaliadas foram: incidência de podridão radicular das plantas de melão no final do ciclo, prevalência dos patógenos e a massa da matéria seca do meloeiro. O feijão-de-porco em cobertura proporciona menor incidência de podridão radicular no meloeiro quando Fusarium solani está sozinho. Macrophomina phaseolina é o fungo que prevalece na maioria das associações nas quais está presente. Aumento na massa da matéria seca do meloeiro foi observado quando aplicado o mulch (filme de polietileno preto) nos tratamentos com associação de F. solani + R. solani e no solo não infestado.

Sign in / Sign up

Export Citation Format

Share Document