scholarly journals Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants

2013 ◽  
Vol 36 (1-2) ◽  
pp. 67-81
Author(s):  
B. Wojciechowska ◽  
D. Czupryńska ◽  
K. Nowak ◽  
A. Ciepliński
Keyword(s):  
Cucumis Sativus ◽  
Callus Culture ◽  
Growth Regulators ◽  
Callus Formation ◽  
Basic Medium ◽  
Cucumis Sativus L ◽  
Hypocotyl Explants ◽  
The Media

Hypocotyl fragments of <i>Cucumis sativus</i> L. were grown on two basic media: Linsmaier-Skoog's (1964) and White's (1943) modified by Street and McGregor (1952). These media were enriched by addition of some growth regulators (NAA, IAA, 2,4-D) in combination with kinetin. White's basic medium is not suitable for callus culture and for rhisogenesis of cucumber hypocotyls. Linsmaier and Skoog's medium was most suitable for growth of callus. After enrichment of the media with IAA, NAA and 2,4-D differences in the degree of taking root by the explants and callus formation were observed.

scholarly journals Stimulation of development of Cucumis sativus L. plants by low concentration of aluminium in vitro conditions

2013 ◽  
Vol 48 (2) ◽  
pp. 75-82
Author(s):  
Maria Szymańska ◽  
Jolanta Molas
Keyword(s):  
Root System ◽  
Cucumis Sativus ◽  
Leaf Explants ◽  
Small Degree ◽  
Basic Medium ◽  
Cucumis Sativus L ◽  
Leaf Formation ◽  
The Media ◽  
Short Shoots

The culture of cucumber plants <em>Cucumis sativus</em> L. Wisconsin cultivar, from seeds and leaf explants, were carried out on the basic medium ofMurashige and Skoog <em>in vitro</em> conditions. In the culture set from the leaf explants the MS medium was supplemented with IAA (0,5 mg/dm<sup>3</sup>) and BAP (2 mg/dm<sup>3</sup>). Aluminium (as AlCl<sub>3</sub>) was added to the media in concentration Of 1 mg/dm<sup>3</sup>. The media pH was adjusted to 6,2 or 4,2. In seedling culture, aluminium substantially stimulated the growth and development of the root system while a shoot to a small degree only (it advanced leaf formation mainly), causing no morphological and developmental anomalies. In the culture from the leaf explants Al induced the process of rhizogenesis which did not take place on the media without Al. It also stimulated a shoot morphogenesis. After 8 weeks of culture, 32 % leafexplants formed plants with short shoots (2-3,5 cm), long ones (5,5-7 cm) and with long but poorly branched root system„ In the acid conditions (pH 4,2), the effect 0 Al on plant growth was Iower than on the media with pH 6,2. Also a number of regenerated explants with comparable direction of differentiation won a fewer in low pH.

Effect of Hypocotyl Length on Morphogenesis of Explants of Cucumber (Cucumis sativus L.) in vitro

1992 ◽  
Vol 19 (2) ◽  
pp. 165
Author(s):  
RL Gambley ◽  
W Dodd
Keyword(s):  
Cucumis Sativus ◽  
Growth Regulators ◽  
Multiple Shoots ◽  
Root Production ◽  
Concomitant Increase ◽  
Hypocotyl Length ◽  
Rapid Reduction ◽  
Cucumis Sativus L ◽  
Murashige And Skoog Medium

Explants of cucumber seedlings having different lengths of hypocotyl attached were grown axenically on Murashige and Skoog medium supplemented with kinetin (2 mg L-1). Multiple shoots developed from the apical regions of all explants. In this tissue shoots may also develop at the base of the hypocotyl, but this response is strongly dependent upon the length of the hypocotyl. As the length of the hypocotyl increased beyond 5 mm, there was a rapid reduction in basal shoot numbers and a concomitant increase in root production. We suggest that these responses are related not to the ratio or concentration of endogenous growth regulators but to different regions of sensitivity to growth regulators along the hypocotyl.

scholarly journals In vitro meristem-tip culture and regeneration approaches in Congolese cassava accessions (Manihot esculenta Crantz cv. Boma and cv. Mpelo-Nlongi)

2015 ◽  
Vol 3 (2) ◽  
pp. 72
Author(s):  
J.-Roger Bansimba Mukiese ◽  
Aimé Diamuini Ndofunsu ◽  
Freddy Bulubulu ◽  
Alexandre Mbaya Ntumbula ◽  
Sébastien Luyindula Ndiku
Keyword(s):  
Growth Regulators ◽  
Manihot Esculenta ◽  
Callus Formation ◽  
Shoot Development ◽  
Media Composition ◽  
Manihot Esculenta Crantz ◽  
Adenine Sulphate ◽  
Meristem Tip Culture ◽  
The Media

<p>Shiny dome-like structures measuring less than 1mm in length were excised aseptically from shoot tip buds of infected of two cassava (<em>Manihot esculenta</em> Crantz) local cultivars (Boma and Mpelo Nlongi) and cultivated <em>in vitro</em> in two types of media with different combination of growth hormone: Murashige and Skoog supplemented of sucrose (20 g/l), Naphtalenacetic acid (NAA, 10 μM), Ben-zylaminopurine (BAP, 0.66 μM) as well as Gibberellic acid (GA3, 0.1 μM) with 80 mg/l of Adenine sulphate and MS-free growth regulators. After four weeks, data were scored: 29.5% responding explant with callus formation and 20.5% responding explants to shoot development in the medium with growth regulators for the cultivar Boma whereas the cultivar Mpelo-Nlongi presented 5.7% and 25.7% respectively of callus formation and shoot development. The cultivar Boma presented a tendency more pronounced for the callus formation rather than with the shoot development contrary to the cultivar Mpelo-Nlongi. In regards of this experiment, it was shown that the media composition and genotype are essential factors, which influence in vitro growth, mainly the shoot development, in the culture of meristems for cassava local accessions.</p>

scholarly journals Factors affecting hemp (Canabis sativa L.) callus genesis in somatic tissue culture

2019 ◽  
Vol 25 (4) ◽  
Author(s):  
Ernestas Maumevičius ◽  
Natalija Burbulis ◽  
Aušra Blinstrubienė ◽  
Irina Laiko ◽  
Ramunė Masienė
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Indoleacetic Acid ◽  
Callus Formation ◽  
Leaf Explants ◽  
Research Centre ◽  
Hypocotyl Explants ◽  
Mean Frequency ◽  
Cotyledon Explants ◽  
The Mean

Research was carried out at the Institute of Biology and Plant Biotechnology of Aleksandras Stulginskis University and at the Laboratory of Agrobiotechnology of the Joint Research Centre. The effect of growth regulators on the callus induction from hypocotyl, cotyledon and leaf explants was evaluated. The isolated explants were cultivated in the MS medium without growth regulators and supplemented with different zeatine (ZT) and α-naftilacetic acid (NAA) or indoleacetic acid (IAA) combinations and thidiazuron (TDZ) and ɑ-naftilacetic acid (NAA) or indoleacetic acid (IAA) combinations. The highest callus formation frequency from hypocotyl explants was obtained in the medium supplemented with 2.0 mg l–1 ZT + 1.0 mg l–1 IAA (‘KAN-30’ and ‘KAN-38’) and – 1.0 mg l–1 ZT + 0.5 mg l–1 NAR (‘KAN-34’). The combination of 2.0 mg l–1 ZT with 1.0 mg l–1 NAA was most appropriate for the callus induction from cotyledon explants. The optimal combination of growth regulators for callus induction from leaf explants should be selected for a specific genotype. The mean rate of callus formation frequency from leaf explants was 4.8–5.6 times higher in comparison with the mean frequency form hypocotyl explants and 2.7–3.1 times higher as compared to the mean frequency from cotyledon explants.

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