Transformation of cucumber ( Cucumis sativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants

1996 ◽  
Vol 15 (11) ◽  
pp. 809-814 ◽  
Author(s):  
Soryu Nishibayashi ◽  
Hiroyasu Kaneko ◽  
Takahiko Hayakawa
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Cucumis Sativus ◽  
Cucumis Sativus L ◽  
Hypocotyl Explants

scholarly journals Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants

2013 ◽  
Vol 36 (1-2) ◽  
pp. 67-81
Author(s):  
B. Wojciechowska ◽  
D. Czupryńska ◽  
K. Nowak ◽  
A. Ciepliński
Keyword(s):  
Cucumis Sativus ◽  
Callus Culture ◽  
Growth Regulators ◽  
Callus Formation ◽  
Basic Medium ◽  
Cucumis Sativus L ◽  
Hypocotyl Explants ◽  
The Media

Hypocotyl fragments of <i>Cucumis sativus</i> L. were grown on two basic media: Linsmaier-Skoog's (1964) and White's (1943) modified by Street and McGregor (1952). These media were enriched by addition of some growth regulators (NAA, IAA, 2,4-D) in combination with kinetin. White's basic medium is not suitable for callus culture and for rhisogenesis of cucumber hypocotyls. Linsmaier and Skoog's medium was most suitable for growth of callus. After enrichment of the media with IAA, NAA and 2,4-D differences in the degree of taking root by the explants and callus formation were observed.

High frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of cucumber (Cucumis sativus L.) seedlings

2000 ◽  
Vol 157 (1) ◽  
pp. 136-139 ◽  
Author(s):  
Jae-Whune Kim ◽  
Su-Kyung Han ◽  
Suk-Yoon Kwon ◽  
Haeng-Soon Lee ◽  
Yong Pyo Lim ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cucumis Sativus ◽  
High Frequency ◽  
Shoot Induction ◽  
Cucumis Sativus L ◽  
Hypocotyl Explants
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