scholarly journals Stimulation of development of Cucumis sativus L. plants by low concentration of aluminium in vitro conditions

2013 ◽  
Vol 48 (2) ◽  
pp. 75-82
Author(s):  
Maria Szymańska ◽  
Jolanta Molas
Keyword(s):  
Root System ◽  
Cucumis Sativus ◽  
Leaf Explants ◽  
Small Degree ◽  
Basic Medium ◽  
Cucumis Sativus L ◽  
Leaf Formation ◽  
The Media ◽  
Short Shoots

The culture of cucumber plants <em>Cucumis sativus</em> L. Wisconsin cultivar, from seeds and leaf explants, were carried out on the basic medium ofMurashige and Skoog <em>in vitro</em> conditions. In the culture set from the leaf explants the MS medium was supplemented with IAA (0,5 mg/dm<sup>3</sup>) and BAP (2 mg/dm<sup>3</sup>). Aluminium (as AlCl<sub>3</sub>) was added to the media in concentration Of 1 mg/dm<sup>3</sup>. The media pH was adjusted to 6,2 or 4,2. In seedling culture, aluminium substantially stimulated the growth and development of the root system while a shoot to a small degree only (it advanced leaf formation mainly), causing no morphological and developmental anomalies. In the culture from the leaf explants Al induced the process of rhizogenesis which did not take place on the media without Al. It also stimulated a shoot morphogenesis. After 8 weeks of culture, 32 % leafexplants formed plants with short shoots (2-3,5 cm), long ones (5,5-7 cm) and with long but poorly branched root system„ In the acid conditions (pH 4,2), the effect 0 Al on plant growth was Iower than on the media with pH 6,2. Also a number of regenerated explants with comparable direction of differentiation won a fewer in low pH.

IN VITRO MORPHOGENESIS OF SHOOTS FROM LEAF EXPLANTS OF CUCUMBER (CUCUMIS SATIVUS L.)

2006 ◽  
pp. 155-160 ◽  
Author(s):  
N. Selvarai ◽  
A. Ganapath ◽  
A. Vasudevan ◽  
G. Vengadesan ◽  
S. Kasthuri Rengan
Keyword(s):  
Cucumis Sativus ◽  
Leaf Explants ◽  
In Vitro Morphogenesis ◽  
Cucumis Sativus L

scholarly journals Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants

2013 ◽  
Vol 36 (1-2) ◽  
pp. 67-81
Author(s):  
B. Wojciechowska ◽  
D. Czupryńska ◽  
K. Nowak ◽  
A. Ciepliński
Keyword(s):  
Cucumis Sativus ◽  
Callus Culture ◽  
Growth Regulators ◽  
Callus Formation ◽  
Basic Medium ◽  
Cucumis Sativus L ◽  
Hypocotyl Explants ◽  
The Media

Hypocotyl fragments of <i>Cucumis sativus</i> L. were grown on two basic media: Linsmaier-Skoog's (1964) and White's (1943) modified by Street and McGregor (1952). These media were enriched by addition of some growth regulators (NAA, IAA, 2,4-D) in combination with kinetin. White's basic medium is not suitable for callus culture and for rhisogenesis of cucumber hypocotyls. Linsmaier and Skoog's medium was most suitable for growth of callus. After enrichment of the media with IAA, NAA and 2,4-D differences in the degree of taking root by the explants and callus formation were observed.

scholarly journals In vitro culture of Cucumis sativus L. VI. Histological analysis of leaf explants cultured on media with 2, 4-D or 2, 4, 5-T

2014 ◽  
Vol 56 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Anna Nadolska-Orczyk ◽  
Stefan Malepszy
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Histological Analysis ◽  
Leaf Explants ◽  
Vascular Bundles ◽  
Meristematic Tissue ◽  
Cucumis Sativus L ◽  
Meristematic Cells ◽  
Cellular Aggregates

The developmental sequence of callus initiation and somatic embryogenesis in leaf explants of <em>Cucumis sativus</em> cv. Borszczagowski was analysed and compared on media containing two different auxin phenoxy-derivatives (2,4-D and 2,4,5-T) and cytokinin (BAP or 2iP). During the first 20 days of culture on media with 2,4,5-T proliferation of parenchymatic tissue occurred mainly and only small meristematic centers were observed. There was an intensive detachment of parenchymatic cells and dissociation of their cell walls near vessels and in the lower part of the explant adjacent to the medium. These cells were strongly plasmolysed. On the 2,4-D containing medium mostly meristematic tissue developed, proliferating around vascular bundles and forming meristematic centers or promeristem-like structures. After 35-50 days of culture, secondary callus was formed by separation of meristematic cells from the meristem surface in explants cultured on the 2,4-D containing medium. On medium supplemented with 2, 4, 5-T the detachment of parenchymatic and meristematic cells occurred, along with formation of a gel-like substance. The gel-like callus contained multi-cellular aggregates, proembryoids and embryoids. This type of callus tissue was initiated more intensively on medium with 2, 4, 5-T, but the frequency of somatic embryogenesis was much lower. The periferial cells of aggregates, proembryoids and embryoids showed the tendency to separate from the surface of the tissue. Many embryoids formed adventitious embryos.

scholarly journals Production of bergenin, an active chemical constituent in the callus of Bergenia ciliata (Haw.) Sternb.

2012 ◽  
Vol 8 ◽  
pp. 40-44 ◽  
Author(s):  
Umesh Krishna Shrestha ◽  
Bijaya Pant
Keyword(s):  
Leaf Explants ◽  
Chemical Constituent ◽  
Wild Plants ◽  
Plant Science ◽  
Naphthalene Acetic Acid ◽  
Active Chemical ◽  
In The Wild ◽  
The Media ◽  
Benzyl Amino Purine

In vitro culture of Bergenia ciliata (Haw.) Sternb. was carried out for the examination of bergenin content. Leaf explants were cultured in MS (Murashige and Skoog) basal media supplemented with or without phytohormones. The hormonal series maintained were in the range of 0-2 mg l-1 for BAP (6-benzyl amino purine) and 0-1.5 mg l-1 for NAA (α-naphthalene acetic acid). Bergenin content of in vitro grown tissues of B. ciliata was compared with that of wild plants collected from three different localities of Nepal. The best growth of callus and plantlets occurred in the media containing BAP 1.0 mg l-1 + NAA 1.0 mg l-1 and BAP 1.5 mg l-1 + NAA 1.0 mg l-1. Production of bergenin was high in the media supplemented with 1.0 mg l-1 BAP + 1.5 mg l-1 NAA (3.40 μg g-1) and 2.0 mg l-1 BAP + 1.5 mg l-1 NAA (3.05 μg g-1) under experimental condition. The bergenin content in the wild plants collected from Langtang, Jumla and Godawari was found to be 4.28 μg g-1, 4.53 μg g-1 and 3.64 μg g-1 respectively. This study shows that the in vitro cultured callus of B. ciliata is capable of synthesizing bergenin in quantity comparable to that of the wild plant.doi: http://dx.doi.org/10.3126/botor.v8i0.5557 Botanica Orientalis – Journal of Plant Science (2011) 8: 40-44

Effect of Ethylene Inhibitors on in vitro Shoot Multiplication and their Impact on Ethylene Production in Cucumber (Cucumis sativus L.)

2006 ◽  
Vol 1 (3) ◽  
pp. 110-117 ◽  
Author(s):  
Vasudevan Ayyappan . ◽  
Selvaraj Natesan . ◽  
Ganapathi Andy . ◽  
Chang won Choi . ◽  
Manickavasagam Marka . ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Ethylene Production ◽  
Shoot Multiplication ◽  
Ethylene Inhibitors ◽  
Cucumis Sativus L

Effect of Hypocotyl Length on Morphogenesis of Explants of Cucumber (Cucumis sativus L.) in vitro

1992 ◽  
Vol 19 (2) ◽  
pp. 165
Author(s):  
RL Gambley ◽  
W Dodd
Keyword(s):  
Cucumis Sativus ◽  
Growth Regulators ◽  
Multiple Shoots ◽  
Root Production ◽  
Concomitant Increase ◽  
Hypocotyl Length ◽  
Rapid Reduction ◽  
Cucumis Sativus L ◽  
Murashige And Skoog Medium

Explants of cucumber seedlings having different lengths of hypocotyl attached were grown axenically on Murashige and Skoog medium supplemented with kinetin (2 mg L-1). Multiple shoots developed from the apical regions of all explants. In this tissue shoots may also develop at the base of the hypocotyl, but this response is strongly dependent upon the length of the hypocotyl. As the length of the hypocotyl increased beyond 5 mm, there was a rapid reduction in basal shoot numbers and a concomitant increase in root production. We suggest that these responses are related not to the ratio or concentration of endogenous growth regulators but to different regions of sensitivity to growth regulators along the hypocotyl.

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