Antimicrobial efficacies of methanol extract of Asteracantha longifolia, Ipomoea aquatica and Enhydra fluctuans against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Micrococcus luteus

10.5580/95a ◽  
2009 ◽  
Vol 7 (2) ◽  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Methanol Extract ◽  
Micrococcus Luteus ◽  
Ipomoea Aquatica

scholarly journals ANÁLISE DA COR E DA ATIVIDADE ANTIMICROBIANA DE MICROCÁPSULAS CONTENDO LACTOFERRINA BOVINA

2014 ◽  
Vol 32 (2) ◽  
Author(s):  
LUCIANA HELENA MAIA PORTE ◽  
MARIA HELENA MIGUEZ ROCHA LEÃO ◽  
ALEXANDRE PORTE
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Spray Drying ◽  
Enterococcus Faecium ◽  
Micrococcus Luteus ◽  
Rhodococcus Equi ◽  
Streptococcus Faecium

Lactoferrina bovina (bLF), proveniente do soro de leite, foimicroencapsulada pela técnica de spray drying. Microcápsulascontendo 20 % de bLF foram produzidas, utilizando-se comomaterial de parede dextrina: amido octenilsuccinato (OSA) emdiferentes proporções: 100:00, 75:25, 50:50, 25:75 e 0:100 %.Foram avaliadas a cor e a estabilidade de cor das microcápsulassob armazenamento em ambientes com diferentes umidadesrelativas e a atividade antimicrobiana da lactoferrina liberadadas microcápsulas. As microcápsulas apresentaram cor clara etenderam a escurecer sob armazenamento em ambiente com altaumidade relativa. Verifi cou-se atividade inibitória das microcápsulasde bLF produzidas para diferentes bactérias Gram positivas(Bacillus subtilis CCT 2576, Staphylococcus aureus CCT 2740,Micrococcus luteus CCT 2692, Enterococcus faecium CCT 5079,Streptococcus faecium ATCC 10541, Rhodococcus equi CCT0541), Gram negativas (Pseudomonas aeruginosa ATCC 13388,Salmonella choleraesius CCT 4296, Escherichia coli CCT 0547) elevedura (Candida albicans ATCC 10231). A concentração inibitóriamínima (MIC) das microcápsulas variou de acordo com o microorganismotestado (MIC entre 2,5-100 mg.mL-1). Com exceção deB. subtilis (MIC entre 50-100 mg.mL-1 para as microcápsulas), aconcentração de bLF contida nas microcápsulas necessária parainibir o crescimento dos micro-organismos foi menor do que a bLFnativa. Esses resultados sugerem efeito de potencialização daatividade antimicrobiana da bLF após o processamento por spraydrying.

scholarly journals Atividade antimicrobiana dos extratos metanólicos de diferentes espécies do gênero Lippia

2021 ◽  
Vol 10 (9) ◽  
pp. e22610918051
Author(s):  
Soraya Castro Trindade ◽  
Jurandi Nery Pereira-Filho ◽  
Laerte Oliveira Barreto Neto ◽  
Thais Brito de Oliveira Moura ◽  
Antonio Pedro Fróes de Farias ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Candida Glabrata ◽  
Micrococcus Luteus ◽  
Salmonella Choleraesuis

As espécies do gênero Lippia (Verbenaceae) possuem diversas moléculas bioativas, oriundas do seu metabolismo secundário, que apresentam atividade antimicrobiana, analgésica, antifúngica e imunomoduladora. Este estudo teve como objetivo avaliar a atividade antimicrobiana in vitro do extrato metanólico das folhas de L. alnifolia, L. origanoides, L. insignis e L. thymoides frente a microrganismos de importância clínica.  Foi realizada uma triagem fitoquímica dos extratos das plantas e a avaliação da atividade antimicrobiana pelo método de difusão em disco, além da determinação da concentração inibitória mínima (MIC) frente às bactérias Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus e Salmonella choleraesuis, e os fungos Candida albicans e Candida glabrata. A presença de saponinas, terpenos, esteroides, cumarinas, ácidos fenólicos e flavonoides foi observada na análise fitoquímica. Os extratos metanólicos das folhas de L. alnifolia, L. origanoides e L. insignis apresentaram atividade antimicrobiana contra todas os microrganismos testados. O extrato metanólico da L. thymoides apresentou atividade antimicrobiana frente aos microrganismos M. luteus, P. aeruginosa, S. aureus, C. albicans e C. glabrata. A maioria dos extratos apresentou atividade bacteriostática e todos os extratos apresentaram atividade fungistática para C. albicans e fungicida para C. glabrata. A utilização dos extratos de Lippia pode ser uma alternativa viável no tratamento de doenças infecciosas de origem bacteriana e fúngica.

scholarly journals Red Cabbage Methanol Extract inhibits the growth of Vancomycin-resistant Enterococcus faecalis during Kirby-Bauer Disk Diffusion Susceptibility Test

2015 ◽  
Vol 4 (1) ◽  
pp. 87-89 ◽  
Author(s):  
Priju Varghese
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methanol Extract ◽  
Micrococcus Luteus ◽  
Vancomycin Resistant Enterococcus ◽  
Zone Of Inhibition ◽  
Red Cabbage ◽  
Vancomycin Resistant

This study was designed to establish whether the red cabbage methanol extract can inhibit the growth of various strains of bacteria which include Micrococcus luteus, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Vancomycin-resistant Enterococcus faecalis (VRE), Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. The red cabbage methanol extract discs were resisted by all the strains of bacteria except for VRE and Enterococcus faecalis. While the antibiotic streptomycin was resisted by VRE, the red cabbage methanol extract caused a remarkable zone of inhibition.

scholarly journals Bacteriological Analysis of Beef Production Chain in Birnin Kebbi Metropolis of Kebbi State, Nigeria

2020 ◽  
pp. 64-76
Author(s):  
Famubo, Joseph A. ◽  
Isiaka, Aishatu ◽  
Abbas, Yusuf B.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Micrococcus Luteus ◽  
Klebsiella Pneumonia ◽  
Proteus Vulgaris ◽  
Production Chain ◽  
Retail Outlets ◽  
Retail Meat

The hygiene status of most abattoirs and retail outlets in Nigeria is very poor, and it contributes to the unacceptable level of bacteria load in beef, and poses a health risk to consumers. This study examined the bacteriological analysis of beef production chain in Birnin Kebbi metropolis. A total of 100 samples of meat, water and surface swab in Main Market, GRA, Badariya, Bayan Kara and Rafin Atiku, Birnin Kebbi Central abattoir and retail meat shops were collected aseptically, processed and analyzed. Meat from retail outlets were mostly contaminated by Staphylococcus aureus (24.4%), followed by Escherichia coli (17.3%), Klebsiella pneumonia (9.8%), Salmonella typhimurium (9.1%), Entrobater aerogenes (8.5%), Streptococcus pyogenes (8.1%), Proteus vulgaris (7.8%), Micrococcus luteus (7.8%) and Pseudomonas aeruginosa (7.2%). In the abattoirs contact surfaces, contamination occurred mostly by Staphylococcus aureus (23.4%), Salmonella typhimurium (18.2%), Escherichia coli (16.9%), Entrobater aerogenes (9.1%), Streptococcus pyogenes (7.8%), Klebsiella pneumonia, Proteus vulgaris, and Pseudomonas aeruginosa (6.5% each) and Micrococcus luteus (5.2%). The findings revealed a high bacterial load in both abattoir and retail meat outlets. In order to safeguard the health of the public against the risks of food borne infections, there is a need to educate and advocate good sanitation and meat handling practices in the abattoir and beef retail outlets.

scholarly journals Synthesis and pharmacological evaluation of some benzylidene-4-nitroanilines

2020 ◽  
Vol 31 (1) ◽  
pp. 55-59
Author(s):  
Ramamurthy Suresh ◽  
Saravanan Palanivel Sakthinathan ◽  
Dakshnamoorthy Kamalakkannan ◽  
Inbasekaran Muthuvel ◽  
Ganesamoorthy Thirunarayanan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Effect ◽  
Micrococcus Luteus ◽  
Ultra Violet ◽  
Fungal Species ◽  
Gram Negative ◽  
Infra Red ◽  
Antibacterial Evaluation

AbstractA number of nine benzylidene-4-nitroanilines were synthesized by condensation method. The formation of the substituted (E)-N-benzylidene-4-nitrobenzenamines has been confirmed from their physical and Ultra-Violet, Infra-Red, NMR spectral data. The evaluation of antimicrobial screening of substituted (E)-N-benzylidene-4-nitrobenzenamines was conducted by using standard Bauer-Kirby method. Three gram-positive microbes namely Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus, and two gram-negative microbes, Escherichia coli and Pseudomonas aeruginosa, were used for the antibacterial evaluation. The antifungal activities against Aspergillus niger and Penicilium scup fungal species were also performed. A good antibacterial effect has been possessed by some of the substituted (E)-N-benzylidene-4-nitrobenzenamines on the microorganisms utilized in the present study

scholarly journals Atividade Antibacteriana, Antioxidante e Compostos Fenólicos de Méis Produzidos por Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)

2021 ◽  
Vol 10 (10) ◽  
pp. e48101018424
Author(s):  
Érica Larissa Silva Carvalho ◽  
Ellen Matos Silva Bomfim ◽  
Marcos da Costa Silva ◽  
Luciene Cristina Lima e Lima ◽  
Edson de Jesus Marques ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Micrococcus Luteus ◽  
Nannotrigona Testaceicornis

O mel de abelhas vem sendo utilizado na medicina popular para o tratamento de infecções e contra danos oxidativos. Entretanto, a composição do mel é bastante variável, pois depende da composição do néctar da espécie vegetal, da espécie de abelha produtora, das condições ambientais, da coleta e processamento. O presente estudo objetivou avaliar a atividade antibacteriana, antioxidante, teor dos compostos fenólicos totais e flavonoides totais do mel de Nannotrigona testaceicornis L. A atividade antibacteriana foi realizada pelo método de difusão em discos e concentração inibitória mínima. A capacidade antioxidante foi investigada através do ensaio de captura do radical livre 2,2-difenil-1-picrilhidrazila. O teor total de fenóis totais e flavonoides totais foi quantificado por espectrofotometria. As amostras dos meses de maio e junho/2013 inibiram o crescimento bacteriano para todas as bactérias analisadas. Todas as amostras de mel apresentaram ação antibacteriana frente a Pseudomonas aeruginosa. O teste ANOVA mostrou que houve diferença estatisticamente significante entre os meses para as bactérias Pseudomonas aeruginosa; Escherichia coli; Micrococcus luteus e Staphylococcus aureus. No ensaio de determinação da concentração inibitória mínima, as amostras de maio/2013, março/2014 e junho/2014 foram mais ativas frente a Micrococcus luteus. A capacidade antioxidante acima de 60% foi observada na amostra de maio/2013. As amostras de janeiro/2014 e maio/2103 se destacaram na quantificação de fenóis totais e flavonoides totais, respectivamente. Houve correlação positiva entre a ação antioxidante e flavonoides totais. O mel in natura de Nannotrigona testaceicornis L. apresenta potencial antimicrobiano e capacidade sequestradora de radicais livres.

scholarly journals Determinación de la composición química del aceite esencial de Thymus vulgaris L. "Tomillo", determinada por cromatografía de fase gaseosa, espectrometría de masa GCIMS y análisis de su actividad antimicrobiana

2000 ◽  
Vol 3 (2) ◽  
pp. 69-78
Author(s):  
Olinda Acosta ◽  
Américo Castro L. ◽  
Mirtha Roque A. ◽  
Luis Felix V.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Staphylococcus Epidermidis ◽  
Micrococcus Luteus ◽  
Salmonella Typhi ◽  
Culture Collection ◽  
American Type Culture Collection ◽  
Thymus Vulgaris

Se ha realizado el estudio del aceite esencial extraído de las hojas y flores de Thymus vulgaris L "Tomillo" por arrastre con vapor de agua. El cual se sometió al análisis físico - químico y determinación de la composición química mediante cromatografía de gases acoplado a un espectrómetro de masas GCIMS, deteuninándose compuestos hidrocarbonados como: Tuyona; 1,3,6-octatrieno, 3,7-dimetil; Canfeno; Betamirceno; Delta-4-careno; Benceno, 1-metil-4-(l-metil etil); Gama terpineno; Beta selineno; Germacreno D; Delta cadineno; Tras-cariofileno; Beta-bisaboleno y compuestos oxigenados como: 7-octen-4-ol (CAS); 1,6-octadieno-3-ol, 3,7-dimetil acetato; Linalol; Isoborneol; carvacrol; Timol; Acetato de famesil 2. Asimismo, se determinó la actividad antimicrobiana frente a cepas patógenas procedentes de muestras clínicas y de la ATCC (American Type Culture Collection), observándose actividad inhibitoria frente a las cepas: Escherichia coli, Escherichia coli, ATCC 25922 Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 6538, Streptococcus β hemolítieo, Staphylococcus epidermidis N° 36, Shigella flexnery, Salmonela paratyphi, Salmonella typhi, Candida albicans ATCC 10231, Candida albicans, Pseudomonas aeruginosa.

scholarly journals Preliminary Investigation of Bioactive Compounds and Bioautographic Studies of Whole Plant Extract of Euphorbia pulcherrima on Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
H. B. Sharif ◽  
M. D. Mukhtar ◽  
Y. Mustapha ◽  
A. O. Lawal
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bioactive Compounds ◽  
Plant Extract ◽  
Methanol Extract ◽  
Preliminary Investigation ◽  
Salmonella Typhi ◽  
Euphorbia Pulcherrima ◽  
Whole Plant

The aim of this study is to carry out preliminary investigation of bioactive compounds and bioautographic studies of whole plant extract of Euphorbia pulcherrima on Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa. Tukey HSD test of hierarchy for the effect of different solvents crude extract on bacterial isolates indicates the methanol extract as the most bioactive. The Tukey HSD analysis also showed that the bioactivities of the crude extracts of the various parts of Euphorbia pulcherrima were part dependent and the whole plant was the most bioactive. The ethyl acetate fraction of the methanol extract of the whole plant of Euphorbia pulcherrima has been shown in this work to contain phytochemicals which have shown remarkable activities against Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa. The bioactivities against the test organisms were due to the combined effects of the compounds separated on TLC plates. Families of terpenoids, flavonoids, alkaloids, saponin, and steroids that were detected in the extracts were identified by GC-MS. The various classes of phytochemicals in the E. pulcherrima plant provided the antimicrobial potency of the plant.

Activité antibactérienne de l’huile essentielle de Pelargonium x asperum et son potentiel synergique avec la nisine

2019 ◽  
Vol 17 (3) ◽  
pp. 140-148 ◽  
Author(s):  
A. Ouelhadj ◽  
L. Ait Salem ◽  
D. Djenane
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Nous Avons ◽  
Activité Antibactérienne

Ce travail vise l’étude de l’activité antibactérienne de l’huile essentielle (HE) de Pelargoniumx asperum et de la bactériocine, la nisine seul et en combinaison vis-à-vis de six bactéries dont quatre sont multirésistantes d’origine clinique. L’activité antibactérienne in vitro a été évaluée par la méthode de diffusion sur gélose. La concentration minimale inhibitrice (CMI) est aussi déterminée pour HE. Les résultats ont révélé une activité antibactérienne significative exercée par HE visà-vis de Staphylococcus aureus (ATCC 43300), Staphylococcus aureus et Escherichia coli avec des diamètres d’inhibition de 36,00 ; 22,50 et 40,00 mm, respectivement. Cependant, l’HE de Pelargonium asperum a montré une activité antibactérienne supérieure par rapport à la nisine. Les valeurs des CMI rapportées dans cette étude sont comprises entre 1,98–3,96 μl/ml. Les combinaisons réalisées entre HE et la nisine ont montré un effet additif vis-à-vis de Escherichia coli (ATCC 25922) avec (50 % HE Pelargonium asperum + 50 % nisine). Par contre, nous avons enregistré une synergie vis-à-vis de Klebsiella pneumoniae avec (75 % HE Pelargonium asperum + 25 % nisine) et contre Pseudomonas aeruginosa avec les trois combinaisons testées. Les résultats obtenus permettent de dire que l’HE de Pelargonium asperum possède une activité antibactérienne ainsi que sa combinaison avec la nisine pourrait représenter une bonne alternative pour la lutte contre l’antibiorésistance.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

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