scholarly journals Preliminary Investigation of Bioactive Compounds and Bioautographic Studies of Whole Plant Extract of Euphorbia pulcherrima on Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
H. B. Sharif ◽  
M. D. Mukhtar ◽  
Y. Mustapha ◽  
A. O. Lawal
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bioactive Compounds ◽  
Plant Extract ◽  
Methanol Extract ◽  
Preliminary Investigation ◽  
Salmonella Typhi ◽  
Euphorbia Pulcherrima ◽  
Whole Plant

The aim of this study is to carry out preliminary investigation of bioactive compounds and bioautographic studies of whole plant extract of Euphorbia pulcherrima on Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa. Tukey HSD test of hierarchy for the effect of different solvents crude extract on bacterial isolates indicates the methanol extract as the most bioactive. The Tukey HSD analysis also showed that the bioactivities of the crude extracts of the various parts of Euphorbia pulcherrima were part dependent and the whole plant was the most bioactive. The ethyl acetate fraction of the methanol extract of the whole plant of Euphorbia pulcherrima has been shown in this work to contain phytochemicals which have shown remarkable activities against Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa. The bioactivities against the test organisms were due to the combined effects of the compounds separated on TLC plates. Families of terpenoids, flavonoids, alkaloids, saponin, and steroids that were detected in the extracts were identified by GC-MS. The various classes of phytochemicals in the E. pulcherrima plant provided the antimicrobial potency of the plant.

scholarly journals Complexations of 2-thiouracil and 2,4-dithiouracil with Cd(SeCN)2and Hg(SeCN)2: NMR and anti-bacterial activity studies

2011 ◽  
Vol 25 (3-4) ◽  
pp. 187-195 ◽  
Author(s):  
M. Nasiruzzaman Shaikh ◽  
Bassem A. Al-Maythalony ◽  
Mohammed I. M. Wazeer ◽  
Anvarhusein A. Isab
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Solid State ◽  
Salmonella Typhi ◽  
Bacterial Activity ◽  
Spectroscopic Techniques ◽  
Standard Type ◽  
Nmr Data ◽  
C Nmr

Cadmium and mercury selenocyanate complexes of 2-thiouracil (TU) and 2,4-dithiouracil (DTU) ligands have been synthesized to form complexes of the type [M(SeCN)2(TU)] and [M(SeCN)2(DTU)] (where M is Cd2+or Hg2+) and studied by various spectroscopic techniques such as IR,1H and13C NMR in solution and in the solid state for13C,15N, and113Cd nuclei. Based on IR, and solution and solid-state13C NMR data, stronger cadmium bonding to the thiouracil was observed compared to that of mercury. Anti-bacterial activities of these complexes have been investigated with standard type culture ofEscherichia coli(MTCC 443),Klebsiella pneumoniae(MTCC 109),Pseudomonas aeruginosa(MTCC 1688),Salmonella typhi(MTCC 733) andStaphylococcus aureus(MTCC 737) and show that ligands exhibit more anti-bacterial activities than that of the corresponding Cd(II) and Hg(II) complexes.

scholarly journals Improvement of the Efficacy an WHO Alcohol-based Hand Rub Formulation by Reducing Isopropanol Content and Associating Syzygium aromarticum and Piper nigrum Essential Oils

2019 ◽  
pp. 1-11
Author(s):  
Mahamat Ismail ◽  
Veronique Penlap ◽  
Wilfred F. Mbacham ◽  
Sylvain Leroy Sado Kamdem
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Essential Oils ◽  
Salmonella Typhi ◽  
World Health ◽  
Partial Substitution ◽  
Piper Nigrum ◽  
Panel Test ◽  
One Year

Aim: This work was carried out in order to reduce the isopropanol contain of a World Health Organization (WHO) basic alcohol based hand rub (ABHR) formulation by substituting part of it with ethanol and essential oils. Study Design: A quasi-experimental design was used, based on WHO basic formulation modification, antimicrobial assessment followed by a panel test and challenge test of the best formulation. Place and Duration of the Study: The study was carried out in different laboratory of the University of Yaoundé 1 during March 2017 to July 2018. Methodology: This study was performed by producing different formulations nested from the WHO basic formulation and testing their antimicrobial capacity on selected strains based on the microbial percentage reduction. Following this, the best formulation was compared to two commercial products trough a panel test and later challenged with selected organism (Staphylococcus aureus SR196, Salmonella Typhi 15SA, Escherichia coli ATTC25922 and Pseudomonas aeruginosa PA01) inoculated in the product after different storage periods for one year and the antimicrobial stability assessed on the same strain for the same length of conservation. Results: The partial substitution of isopropanol with ethanol and Piper nigrum and Syzygium aromaticum essential oils proved to possess more antimicrobial properties than the original WHO formulation. The best formulation caused a 6 Log cells/ml reduction of the initial population compared to the 4 Log cells/ml of the WHO formulation. The product also proved to maintain its activity for one year and to be able to deactivate possible contaminations by Salmonella Typhi 15SA, Staphylococcus aureus NCTC10652, Escherichia coli ATCC25922, Pseudomonas aeruginosa PA01. Conclusion: The present work is a contribution to the improvement of ABHRs and could permit the reduction of hand hygiene associated infections in industries and health care facilities.

scholarly journals Aktivitas Antibakteri dari Damar Batu (Shorea eximia) asal Indonesia

2012 ◽  
Vol 17 (1) ◽  
Author(s):  
Noryawati Mulyono ◽  
Bibiana Widiyati Laya ◽  
Siuling Susanti Rusli
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Proteus Mirabilis ◽  
Salmonella Typhi

Damar batu adalah resin natural, atau lebih tepatnya adalah hasil hutan bukan kayu dari tumbuhan Shorea eximia. Getah ini dihasilkan sebagai metabolit sekunder yang diinduksi oleh malnutrisi dan kekeringan. Penelitian ini bertujuan melakukan evaluasi aktivitas antibakteri dalam metabolit sekunder tersebut. Resin dilarutkan dalam heksana, etil asetat, dan etanol secara terpisah selama 24 jam dengan konsentrasi 0,5 g L -1 . Selanjutnya, aktivitas antibakteri diuji terhadap Escherichia coli, Salmonella typhi, Proteus mirabilis, Pseudomonas aeruginosa, Chromobacter violaceum, Streptococcus sp., Staphylococcus aureus, S. epidermidis, dan Bacillus cereus. Tiga komponen utama dalam damar batu yang mempunyai aktivitas antibakteri adalah δ-kadinen, valencene, dan spatulenol.

Activité antimicrobienne de l’huile essentielle de galles du Pistachier de l’Atlas (Pistacia atlantica Desf.) en Algérie

2019 ◽  
Author(s):  
I. Sifi ◽  
M. Yousfi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Bacillus Cereus ◽  
Salmonella Typhi ◽  
Huiles Essentielles ◽  
Activité Antimicrobienne ◽  
Pistacia Atlantica

L’activité antimicrobienne des huiles essentielles de galles de Pistacia atlantica récoltées dans trois régions (Aïn-Oussara, Laghouat et Kheneg) a été évaluée sur sept souches pathogènes (Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, Staphylococcus aureus, MRSA+, Salmonella typhi et Candida albicans), en utilisant les méthodes de diffusion sur gélose, de microdilution et celle de microatmosphère (seulement pour Candida albicans). Les huiles essentielles ont révélé un effet antimicrobien remarquable contre les micro-organismes testés, avec des valeurs de CMI et de CMB variant de 0,13 à 7,99 μl/ml et de 0,25 à 7,99 μl/ml, respectivement. Une activité importante a été observée pour les échantillons de la station Kheneg contre l’espèce Candida albicans avec une zone d’inhibition de 68 mm (50 μl). Ces résultats suggèrent que les huiles essentielles testées pour leur activité antimicrobienne peuvent être classées comme bactéricides.

scholarly journals Pengujian Aktivitas Antibakteri Ekstrak Kulit Ranting Sengon Dengan Pelarut Metanol Dan N-Heksana

2020 ◽  
Vol 3 (1) ◽  
pp. 6-15
Author(s):  
Nunik Tri Rahayu ◽  
Ai Sri Nurhasanah ◽  
Alfi Rumidatul ◽  
Feldha Fadhila ◽  
Yayan Maryana
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Proteus Mirabilis ◽  
Salmonella Typhi

Pohon Sengon (Falcataria moluccana) memiliki senyawa metabolit sekunder yaitu terpenoid, steroid, flavonoid, fenolik, tanin, saponin yang berfungsi sebagai antibakteri. Antibakteri bersumber dari alam menjadi alternatif untuk pengobatan penyakit infeksi. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak kulit ranting Sengon dengan pelarut n-heksana dan metanol terhadap Shigella dysentriae, Klebsiella pneumoniae, Escherichia coli, Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa, dan Proteus mirabilis. Penelitian ini menggunakan metode Kirby Bauer dengan konsentrasi ekstrak 9%, 9,5%, 10%, 10,5% dan 11%. Hasil ekstrak kulit ranting Sengon menunjukkan adanya zona bening dengan konsentrasi efektif yaitu 11% terhadap Shigella dysentriae (4 mm), Escherichia coli (1,7 mm), Salmonella typhi (3,3 mm). Sedangkan ekstrak kulit ranting Sengon dengan pelarut n-heksana terhadap Proteus mirabillis (2,7 mm). Ekstrak kulit ranting Sengon dengan pelarut metanol dan n-heksana memiliki aktivitas antibakteri terhadap Shigella dysentriae, Escherichia coli, Salmonella typhi, dan Proteus mirabilis. Kata kunci: Antibakteri, Falcataria moluccana, N-heksana, Metanol

scholarly journals Antibacterial and Phytochemical Properties of Crude Leaf Extracts of Moringa oleifera Lam., Pterocarpus santalinoides L’Herit DC and Ceiba pentandra L. on Some Clinical Bacterial Isolates in Nigeria

2020 ◽  
pp. 1-15
Author(s):  
Reginald C. Njokuocha ◽  
Anthonia E. Ewenike
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Salmonella Typhi ◽  
Bacterial Isolates ◽  
Leaf Extracts ◽  
Ceiba Pentandra ◽  
Ethanolic Extracts ◽  
Pterocarpus Santalinoides

Aims: The study was carried out to determine the phytochemical constituents and antibacterial activity of aqueous and ethanolic extracts of fresh leaves of Moringa oleifera Lam., Pterocarpus santalinoides L’Herit DC and Ceiba pentandra L. on bacterial isolates; Salmonella typhi, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Methodology: The plant leaves were dried, pulverized and phytochemical tests were done according to standard laboratory procedure. Aqueous and ethanolic extracts were obtained from 20 g of the of the ground leaves. Antibacterial assay was carried out with Disc diffusion method on seven concentrations of the extracts ;100,50,25,12.5, 6.25,3.125,1.5625 mg/ml and compared with standard antibiotics. Isolated bacterial pathogens; Salmonella typhi, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa (1.0 x 105 cfu /ml) were used as test organisms. Results: Alkaloids, steroidal aglycones, glycosides, proteins, carbohydrates, reducing sugars, tannins, saponins, vitamins A and E were present in all the plant samples. Flavonoids and cardiac glycosides were not detected in Pterocarpus santalinoides and Ceiba pentandra, respectively. Anthracene glycoside was absent in all samples. Aqueous and ethanolic extracts of M. oleifera showed antibacterial activities against all the bacterial isolates at minimum inhibitory concentration (MIC) of 3.125 mg/ml and 1.5625 mg/ml respectively. Pterocarpus santalinoides showed inhibitory activity only on Salmonella typhi at 3.125 mg/ml and Escherichia coli 1.5625 mg/ml MIC. Ceiba pentandra showed spectrum of antibacterial activity against all the bacterial isolates at 1.56 mg/ml MIC with exception of Salmonella typhi. E. coli was the most susceptible to the leaf extracts. Salmonella typhi was not sensitive to the leaf extracts of Ceiba pentandra, while Staphylococcus aureus and Pseudomonas aeruginosa were not sensitive to the leaf extracts of Pterocarpus santalinoides. Conclusion: It can be concluded that both aqueous and ethanolic leaf extracts had antibacterial activity against the test organism, thus justifying their use in folklore medicine.

scholarly journals Antibacterial Screening of Fifteen Cameroonian Medicinal Plants against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi

2016 ◽  
Vol 4 (10) ◽  
Author(s):  
Elisabeth Zeuko’o Menkem ◽  
Rufin Marie Toghueo Kouipou ◽  
Cedric Derick Jiatsa Mbouna ◽  
Maguerite Simo Kamdem ◽  
Patrick Valere Tsouh Fokou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Medicinal Plants ◽  
Salmonella Typhi ◽  
Antibacterial Screening

scholarly journals UJI DAYA HAMBAT EKSTRAK RIMPANG SEGAR KUNYIT PUTIH (Curcuma zedoaria, Berg) TERHADAP PERTUMBUHAN KUMAN GRAM POSITIF DAN GRAM NEGATIF IN VITRO

2015 ◽  
Vol 3 (1) ◽  
pp. 50
Author(s):  
Elizabeth Bahar
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Cancer Cell ◽  
Salmonella Typhi ◽  
Gram Negative Bacteria ◽  
Curcuma Zedoaria ◽  
Test Sensitivity ◽  
Anti Cancer

 ABSTRACT Known as white turmeric crop of crop medicines anti cancer where this crop rimpang contain immeasurable chemical vitamin in the reality can pursue many of cancer cell. Though unegual germ cell its structure with cancer cell but process many of is same cell perform a replicace. Have been done/conducted by research of test sensitivity of extract of rimpang fresh of white turmeric in so many concentration 100%, 75%, 50% and 25% to growth of germ of Staphylococcus aureus, Salmonella typhii, Pseudomonas aeruginosa dan Escherichia coli of in vitro to know its sensitivity. Research of experiment by using factorial pattern in complete random device (RAL) with 5 restating. From result of research obtained by extract of rimpang fresh white tumeric in so many concentration not pursue growth of germ of Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa dan Escherichia coli. Its conclusion of extract of rimpang fresh of white turmeric non influence growth of Gram positive and Gram negative bacteria. Keywords: extract, fresh rimpang, white turmeric, germ

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