The Relationship Between Postmortem Interval And Dna Degradation In Different Tissues Of Drowned Rats

10.5580/1293 ◽  
2009 ◽  
Vol 4 (1) ◽  
Keyword(s):  
Dna Degradation ◽  
Postmortem Interval ◽  
The Relationship ◽  
Different Tissues

scholarly journals The Relationship between Postmortem Interval and DNA Degradation in Different Tissues of Drowned Rats

2008 ◽  
Vol 16 (2) ◽  
pp. 45-61 ◽  
Author(s):  
Mona El-Harouny ◽  
Sahar El-Dakroory ◽  
Sohayla Attalla ◽  
Nermin, Hasan ◽  
Sobhy El-Nabi
Keyword(s):  
Dna Degradation ◽  
Postmortem Interval ◽  
The Relationship ◽  
Different Tissues

scholarly journals Metabolite profile comparison of a graft chimera ‘Hongrou Huyou’ (Citrus changshan-huyou + Citrus unshiu) and its two donor plants

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Min Zhang ◽  
Luyang Jing ◽  
Qun Wu ◽  
Kaijie Zhu ◽  
Fuzhi Ke ◽  
...  
Keyword(s):  
Principal Component ◽  
Metabolite Profile ◽  
Citrus Unshiu ◽  
Primary Metabolites ◽  
Citrus Breeding ◽  
Metabolite Profiles ◽  
Germacrene D ◽  
The Relationship ◽  
Different Tissues ◽  
Juice Sacs

Abstract Background Chimeras synthesized artificially by grafting are crucial to the breeding of perennial woody plants. ‘Hongrou Huyou’ (Citrus changshan-huyou + Citrus unshiu) is a new graft chimera originating from the junction where a Citrus changshan-huyou (“C”) scion was top-grafted onto a stock Satsuma mandarin ‘Owari’ (C. unshiu, “O”). The chimera was named OCC because the cell layer constitutions were O for Layer 1(L1) and C for L2 and L3. In this study, profiles of primary metabolites, volatiles and carotenoids derived from different tissues in OCC and the two donors were investigated, with the aim of determining the relationship between the layer donors and metabolites. Results The comparison of the metabolite profiles showed that the amount and composition of metabolites were different between the peels and the juice sacs, as well as between OCC and each of the two donors. The absence or presence of specific metabolites (such as the carotenoids violaxanthin and β-cryptoxanthin, the volatile hydrocarbon germacrene D, and the primary metabolites citric acid and sorbose) in each tissue was identified in the three phenotypes. According to principal component analysis (PCA), overall, the metabolites in the peel of the chimera were derived from donor C, whereas those in the juice sac of the chimera came from donor O. Conclusion The profiles of primary metabolites, volatiles and carotenoids derived from the peels and juice sacs of OCC and the two donors were systematically compared. The content and composition of metabolites were different between the tissues and between OCC and the each of the two donors. A clear donor dominant pattern of metabolite inheritance was observed in the different tissues of OCC and was basically consistent with the layer origin; the peel of the chimera was derived from C, and the juice sacs of the chimera came from O. These profiles provide potential chemical markers for genotype differentiation, citrus breeding assessment, and donor selection during artificial chimera synthesis.

scholarly journals Detection of Type I and III collagen in porcine acellular matrix using HPLC–MS

2020 ◽  
Vol 7 (6) ◽  
pp. 577-582
Author(s):  
Yang Zhang ◽  
Yi Chen ◽  
Bo Zhao ◽  
Jianping Gao ◽  
Leilei Xia ◽  
...  
Keyword(s):  
Type I ◽  
Triple Quadrupole Mass Spectrometer ◽  
Collagen Concentration ◽  
Acellular Matrix ◽  
Different Types ◽  
Small Intestinal ◽  
Main Component ◽  
Intestinal Submucosa ◽  
The Relationship ◽  
Different Tissues

Abstract Acellular matrix (ACM) has been widely used as a biomaterial. As the main component of ACM, collagen type and content show influence on the material properties. In this research, the collagen in ACM from different tissues of pig were determined by detection of marker peptides. The marker peptides of Type I and III collagen were identified from the digested collagen standards using ions trap mass spectrometry (LCQ). The relationship between the abundance of marker peptide and collagen concentration was established using triple quadrupole mass spectrometer (TSQ). The contents of Type I and III collagen in ACM from different tissues were determined. The method was further verified by hydroxyproline determination. The results showed that, the sum of Type I and III collagen contents in the ACM from small intestinal submucosa, dermis and Achilles tendon of pig were about 87.59, 81.41 and 61.13%, respectively, which were close to the total collagen contents in these tissues. The results proved that this method could quantitatively detect the collagen with different types in the ACM of various tissues.

scholarly journals Metabolite profile comparison of a graft chimera ‘Hongrou Huyou’ (Citrus changshan-huyou + Citrus unshiu) and its two donor plants

2019 ◽  
Author(s):  
Min Zhang ◽  
Luyang Jing ◽  
Qun Wu ◽  
Kaijie Zhu ◽  
Fuzhi Ke ◽  
...  
Keyword(s):  
Principal Component ◽  
Metabolite Profile ◽  
Citrus Unshiu ◽  
Primary Metabolites ◽  
Citrus Breeding ◽  
Metabolite Profiles ◽  
Germacrene D ◽  
The Relationship ◽  
Different Tissues ◽  
Juice Sacs

Abstract Background: Chimeras synthesized artificially by grafting are crucial to the breeding of perennial woody plants. ‘Hongrou Huyou’ (Citrus changshan-huyou + Citrus unshiu) is a new graft chimera originating from the junction where a Citrus changshan-huyou (“C”) scion was top-grafted onto a stock Satsuma mandarin ‘Owari’ (C. unshiu, “O”). The chimera was named OCC because the cell layer constitutions were O for Layer 1(L1) and C for L2 and L3. In this study, profiles of primary metabolites, volatiles and carotenoids derived from different tissues in OCC and the two donors were investigated, with the aim of determining the relationship between the layer donors and metabolites. Results: The comparison of the metabolite profiles showed that the amount and composition of metabolites were different between the peels and the juice sacs, as well as between OCC and each of the two donors. The absence or presence of specific metabolites (such as the carotenoids violaxanthin and β-cryptoxanthin, the volatile hydrocarbon germacrene D, and the primary metabolites citric acid and sorbose) in each tissue was identified in the three phenotypes. According to principal component analysis (PCA), overall, the metabolites in the peel of the chimera were derived from donor C, whereas those in the juice sac of the chimera came from donor O. Conclusion: The profiles of primary metabolites, volatiles and carotenoids derived from the peels and juice sacs of OCC and the two donors were systematically compared. The content and composition of metabolites were different between the tissues and between OCC and the each of the two donors. A clear donor dominant pattern of metabolite inheritance was observed in the different tissues of OCC and was basically consistent with the layer origin; the peel of the chimera was derived from C, and the juice sacs of the chimera came from O. These profiles provide potential chemical markers for genotype differentiation, citrus breeding assessment, and donor selection during artificial chimera synthesis.

scholarly journals Evaluation of Parameters for Estimating the Postmortem Interval of Skeletal Remains Using Bovine Femurs: A Pilot Study

Diagnostics ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1066
Author(s):  
Midori Nagai ◽  
Koichi Sakurada ◽  
Kazuhiko Imaizumi ◽  
Yoshinori Ogawa ◽  
Motohiro Uo ◽  
...  
Keyword(s):  
Environmental Conditions ◽  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Dna Degradation ◽  
Postmortem Interval ◽  
Skeletal Remains ◽  
Emission Spectrometry ◽  
Inductively Coupled ◽  
Element Concentrations ◽  
Wide Range

The postmortem interval (PMI) of victims is a key parameter in criminal investigations. However, effective methods for estimating the PMI of skeletal remains have not been established because it is determined by various factors, including environmental conditions. To identify effective parameters for estimating the PMI of skeletal remains, we investigated the change in bone focusing on the amount of DNA, element concentrations, and bone density that occurred in the bone samples of bovine femurs, each maintained under one of five simulated environmental conditions (seawater, freshwater, underground, outdoors, and indoors) for 1 year. The amount of extracted mitochondrial DNA (mtDNA; 404 bp fragment) decreased over time, and significant DNA degradation (p < 0.01), as estimated by a comparison with amplification results for a shorter fragment (128 bp), was detected between 1 month and 3 months. Eleven of 30 elements were detected in samples by inductively coupled plasma optical emission spectrometry, and Na and Ba showed significant quantitative differences in terms of environmental conditions and time (p < 0.01). This preliminary study suggests that the level of DNA degradation determined by real-time polymerase chain reaction and element concentrations determined by inductively coupled plasma optical emission may be useful indices for estimating the PMI of victims under a wide range of environmental conditions. However, this study is a limited experimental research and not applicable to forensic cases as it is. Further studies of human bone with longer observation periods are required to verify these findings and to establish effective methods for PMI estimation.

The relationship between adrenal gland morphometric changes and postmortem interval in rats: A stereological study

2012 ◽  
Vol 14 (4) ◽  
pp. 214-218 ◽  
Author(s):  
Ayse Kurtulus ◽  
Kemalettin Acar ◽  
Hulya Sorkun ◽  
Canan Kelten ◽  
Bora Boz
Keyword(s):  
Adrenal Gland ◽  
Postmortem Interval ◽  
The Relationship

scholarly journals Endothelial Cells as Tools to Model Tissue Microenvironment in Hypoxia-Dependent Pathologies

2021 ◽  
Vol 22 (2) ◽  
pp. 520
Author(s):  
Aleksandra Majewska ◽  
Kinga Wilkus ◽  
Klaudia Brodaczewska ◽  
Claudine Kieda
Keyword(s):  
Endothelial Cells ◽  
Alternative Methods ◽  
Biological Processes ◽  
Animal Testing ◽  
Tissue Microenvironment ◽  
Vessel Permeability ◽  
Biological Phenomena ◽  
The Relationship ◽  
Different Tissues

Endothelial cells (ECs) lining the blood vessels are important players in many biological phenomena but are crucial in hypoxia-dependent diseases where their deregulation contributes to pathology. On the other hand, processes mediated by ECs, such as angiogenesis, vessel permeability, interactions with cells and factors circulating in the blood, maintain homeostasis of the organism. Understanding the diversity and heterogeneity of ECs in different tissues and during various biological processes is crucial in biomedical research to properly develop our knowledge on many diseases, including cancer. Here, we review the most important aspects related to ECs’ heterogeneity and list the available in vitro tools to study different angiogenesis-related pathologies. We focus on the relationship between functions of ECs and their organo-specificity but also point to how the microenvironment, mainly hypoxia, shapes their activity. We believe that taking into account the specific features of ECs that are relevant to the object of the study (organ or disease state), especially in a simplified in vitro setting, is important to truly depict the biology of endothelium and its consequences. This is possible in many instances with the use of proper in vitro tools as alternative methods to animal testing.

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