Correlative analysis on the relationship between PMI and DNA degradation of cell nucleus in human different tissues

2005 ◽  
Vol 25 (4) ◽  
pp. 423-426 ◽  
Author(s):  
Shu Xiji ◽  
Liu Yaling ◽  
Ren Liang ◽  
He Fanggang ◽  
Zhou Hongyan ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Dna Degradation ◽  
Correlative Analysis ◽  
The Relationship ◽  
Different Tissues

scholarly journals The Relationship between Postmortem Interval and DNA Degradation in Different Tissues of Drowned Rats

2008 ◽  
Vol 16 (2) ◽  
pp. 45-61 ◽  
Author(s):  
Mona El-Harouny ◽  
Sahar El-Dakroory ◽  
Sohayla Attalla ◽  
Nermin, Hasan ◽  
Sobhy El-Nabi
Keyword(s):  
Dna Degradation ◽  
Postmortem Interval ◽  
The Relationship ◽  
Different Tissues

scholarly journals Metabolite profile comparison of a graft chimera ‘Hongrou Huyou’ (Citrus changshan-huyou + Citrus unshiu) and its two donor plants

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Min Zhang ◽  
Luyang Jing ◽  
Qun Wu ◽  
Kaijie Zhu ◽  
Fuzhi Ke ◽  
...  
Keyword(s):  
Principal Component ◽  
Metabolite Profile ◽  
Citrus Unshiu ◽  
Primary Metabolites ◽  
Citrus Breeding ◽  
Metabolite Profiles ◽  
Germacrene D ◽  
The Relationship ◽  
Different Tissues ◽  
Juice Sacs

Abstract Background Chimeras synthesized artificially by grafting are crucial to the breeding of perennial woody plants. ‘Hongrou Huyou’ (Citrus changshan-huyou + Citrus unshiu) is a new graft chimera originating from the junction where a Citrus changshan-huyou (“C”) scion was top-grafted onto a stock Satsuma mandarin ‘Owari’ (C. unshiu, “O”). The chimera was named OCC because the cell layer constitutions were O for Layer 1(L1) and C for L2 and L3. In this study, profiles of primary metabolites, volatiles and carotenoids derived from different tissues in OCC and the two donors were investigated, with the aim of determining the relationship between the layer donors and metabolites. Results The comparison of the metabolite profiles showed that the amount and composition of metabolites were different between the peels and the juice sacs, as well as between OCC and each of the two donors. The absence or presence of specific metabolites (such as the carotenoids violaxanthin and β-cryptoxanthin, the volatile hydrocarbon germacrene D, and the primary metabolites citric acid and sorbose) in each tissue was identified in the three phenotypes. According to principal component analysis (PCA), overall, the metabolites in the peel of the chimera were derived from donor C, whereas those in the juice sac of the chimera came from donor O. Conclusion The profiles of primary metabolites, volatiles and carotenoids derived from the peels and juice sacs of OCC and the two donors were systematically compared. The content and composition of metabolites were different between the tissues and between OCC and the each of the two donors. A clear donor dominant pattern of metabolite inheritance was observed in the different tissues of OCC and was basically consistent with the layer origin; the peel of the chimera was derived from C, and the juice sacs of the chimera came from O. These profiles provide potential chemical markers for genotype differentiation, citrus breeding assessment, and donor selection during artificial chimera synthesis.

scholarly journals The Effects of Chinese Seafarers’ Job Demands on Turnover Intention: The Role of Fun at Work

2020 ◽  
Vol 17 (14) ◽  
pp. 5247
Author(s):  
Yuan Gu ◽  
Dongbei Liu ◽  
Guoping Zheng ◽  
Chuanyong Yang ◽  
Zhen Dong ◽  
...  
Keyword(s):  
Turnover Intention ◽  
Online Survey ◽  
Turnover Intentions ◽  
Job Demands ◽  
Occupational Commitment ◽  
Group Activities ◽  
Correlative Analysis ◽  
The Relationship ◽  
Chinese Seafarers

This study examines how an occupational commitment and a fun work environment serve as important mechanisms that influence the job demands–turnover intentions relationship. On the basis of the job demands–resources model, the study explored the relationship between job demands, occupational commitment, fun at work, and turnover intention. The hypotheses were (1) that job demands would be positively associated with predicted turnover intention; (2) that occupational commitment would mediate the job demands–turnover intention link and (3) that a fun environment would moderate the relationship between job demands and occupational commitment and between job demands and turnover intention. The study sampled 294 seafarers using an online survey, and applied descriptive, correlative analysis and the PROCESS Macro to test the hypotheses. Findings provide preliminary support for the three hypotheses, and contribute to a better understanding of the mechanism determining seafarers’ turnover intention. The results suggest the importance of holding appropriate group activities on-board to help seafarers alleviate fatigue and stress.

scholarly journals Detection of Type I and III collagen in porcine acellular matrix using HPLC–MS

2020 ◽  
Vol 7 (6) ◽  
pp. 577-582
Author(s):  
Yang Zhang ◽  
Yi Chen ◽  
Bo Zhao ◽  
Jianping Gao ◽  
Leilei Xia ◽  
...  
Keyword(s):  
Type I ◽  
Triple Quadrupole Mass Spectrometer ◽  
Collagen Concentration ◽  
Acellular Matrix ◽  
Different Types ◽  
Small Intestinal ◽  
Main Component ◽  
Intestinal Submucosa ◽  
The Relationship ◽  
Different Tissues

Abstract Acellular matrix (ACM) has been widely used as a biomaterial. As the main component of ACM, collagen type and content show influence on the material properties. In this research, the collagen in ACM from different tissues of pig were determined by detection of marker peptides. The marker peptides of Type I and III collagen were identified from the digested collagen standards using ions trap mass spectrometry (LCQ). The relationship between the abundance of marker peptide and collagen concentration was established using triple quadrupole mass spectrometer (TSQ). The contents of Type I and III collagen in ACM from different tissues were determined. The method was further verified by hydroxyproline determination. The results showed that, the sum of Type I and III collagen contents in the ACM from small intestinal submucosa, dermis and Achilles tendon of pig were about 87.59, 81.41 and 61.13%, respectively, which were close to the total collagen contents in these tissues. The results proved that this method could quantitatively detect the collagen with different types in the ACM of various tissues.

scholarly journals Personalized Bioconversion of Panax Notoginseng Saponins Mediated by Gut Microbiota Between Chinese-diet and Western-diet Healthy Subjects

2021 ◽  
Author(s):  
Li Wang ◽  
Man-Yun Chen ◽  
Li Shao ◽  
Wei Zhang ◽  
Xiang-Ping Li ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Lactobacillus Rhamnosus ◽  
16S Rrna Gene Sequencing ◽  
Panax Notoginseng ◽  
Rrna Gene ◽  
Bifidobacterium Adolescentis ◽  
Correlative Analysis ◽  
Rrna Gene Sequencing ◽  
The Relationship

Abstract Background: Panax notoginseng saponins (PNS) as the main effective substances from P. notoginseng with low bioavailability could be bio-converted by human gut microbiota. In our previous study, PNS metabolic variations mediated by gut microbiota have been observed between high fat, high protein (HF-HP)-diet and low fat, plant fiber-rich (LF-PF)-diet subjects. In this study, we aimed to correspondingly characterize the relationship between distinct gut microbiota profiles and PNS metabolites. Methods: Gut microbiota were collected from HF-HP and LF-PF healthy adults, respectively and profiled by 16S rRNA gene sequencing. PNS were incubated with gut microbiota in vitro. A LC-MS/MS method was developed to quantify the five main metabolites yields including ginsenoside F1 (GF1), ginsenoside Rh2 (GRh2), ginsenoside compound K (GC-K), protopanaxatriol (PPT) and protopanaxadiol (PPD). The selected microbial species, Bifidobacterium adolescentis and Lactobacillus rhamnosus, were employed to metabolize PNS for the corresponding metabolites.Results: The five main metabolites were significantly different between the two diet groups. Compared with HF-HP group, the microbial genus Blautia, Bifidobacterium, Clostridium, Corynebacterium, Dorea, Enhydrobacter, Lactobacillus, Roseburia, Ruminococcus, SMB53, Streptococcus, Treponema and Weissella were enriched in LF-PF group, while Phascolarctobacterium and Oscillospira were relatively decreased. Furthermore, Spearman’s correlative analysis revealed gut microbiota enriched in LF-PF and HF-HP groups were positively and negatively associated with PNS metabolites yields, respectively. Conclusions: Our data showed gut microbiota diversity led to the personalized bioconversion of PNS.

scholarly journals Metabolite profile comparison of a graft chimera ‘Hongrou Huyou’ (Citrus changshan-huyou + Citrus unshiu) and its two donor plants

2019 ◽  
Author(s):  
Min Zhang ◽  
Luyang Jing ◽  
Qun Wu ◽  
Kaijie Zhu ◽  
Fuzhi Ke ◽  
...  
Keyword(s):  
Principal Component ◽  
Metabolite Profile ◽  
Citrus Unshiu ◽  
Primary Metabolites ◽  
Citrus Breeding ◽  
Metabolite Profiles ◽  
Germacrene D ◽  
The Relationship ◽  
Different Tissues ◽  
Juice Sacs

Abstract Background: Chimeras synthesized artificially by grafting are crucial to the breeding of perennial woody plants. ‘Hongrou Huyou’ (Citrus changshan-huyou + Citrus unshiu) is a new graft chimera originating from the junction where a Citrus changshan-huyou (“C”) scion was top-grafted onto a stock Satsuma mandarin ‘Owari’ (C. unshiu, “O”). The chimera was named OCC because the cell layer constitutions were O for Layer 1(L1) and C for L2 and L3. In this study, profiles of primary metabolites, volatiles and carotenoids derived from different tissues in OCC and the two donors were investigated, with the aim of determining the relationship between the layer donors and metabolites. Results: The comparison of the metabolite profiles showed that the amount and composition of metabolites were different between the peels and the juice sacs, as well as between OCC and each of the two donors. The absence or presence of specific metabolites (such as the carotenoids violaxanthin and β-cryptoxanthin, the volatile hydrocarbon germacrene D, and the primary metabolites citric acid and sorbose) in each tissue was identified in the three phenotypes. According to principal component analysis (PCA), overall, the metabolites in the peel of the chimera were derived from donor C, whereas those in the juice sac of the chimera came from donor O. Conclusion: The profiles of primary metabolites, volatiles and carotenoids derived from the peels and juice sacs of OCC and the two donors were systematically compared. The content and composition of metabolites were different between the tissues and between OCC and the each of the two donors. A clear donor dominant pattern of metabolite inheritance was observed in the different tissues of OCC and was basically consistent with the layer origin; the peel of the chimera was derived from C, and the juice sacs of the chimera came from O. These profiles provide potential chemical markers for genotype differentiation, citrus breeding assessment, and donor selection during artificial chimera synthesis.

scholarly journals Endothelial Cells as Tools to Model Tissue Microenvironment in Hypoxia-Dependent Pathologies

2021 ◽  
Vol 22 (2) ◽  
pp. 520
Author(s):  
Aleksandra Majewska ◽  
Kinga Wilkus ◽  
Klaudia Brodaczewska ◽  
Claudine Kieda
Keyword(s):  
Endothelial Cells ◽  
Alternative Methods ◽  
Biological Processes ◽  
Animal Testing ◽  
Tissue Microenvironment ◽  
Vessel Permeability ◽  
Biological Phenomena ◽  
The Relationship ◽  
Different Tissues

Endothelial cells (ECs) lining the blood vessels are important players in many biological phenomena but are crucial in hypoxia-dependent diseases where their deregulation contributes to pathology. On the other hand, processes mediated by ECs, such as angiogenesis, vessel permeability, interactions with cells and factors circulating in the blood, maintain homeostasis of the organism. Understanding the diversity and heterogeneity of ECs in different tissues and during various biological processes is crucial in biomedical research to properly develop our knowledge on many diseases, including cancer. Here, we review the most important aspects related to ECs’ heterogeneity and list the available in vitro tools to study different angiogenesis-related pathologies. We focus on the relationship between functions of ECs and their organo-specificity but also point to how the microenvironment, mainly hypoxia, shapes their activity. We believe that taking into account the specific features of ECs that are relevant to the object of the study (organ or disease state), especially in a simplified in vitro setting, is important to truly depict the biology of endothelium and its consequences. This is possible in many instances with the use of proper in vitro tools as alternative methods to animal testing.

scholarly journals Pyroptosis: mechanisms and diseases

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Pian Yu ◽  
Xu Zhang ◽  
Nian Liu ◽  
Ling Tang ◽  
Cong Peng ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Side Effects ◽  
Treatment Strategy ◽  
New Drugs ◽  
Basic Knowledge ◽  
Tumor Treatment ◽  
Dual Effect ◽  
Rational Use ◽  
The Relationship ◽  
Different Tissues

AbstractCurrently, pyroptosis has received more and more attention because of its association with innate immunity and disease. The research scope of pyroptosis has expanded with the discovery of the gasdermin family. A great deal of evidence shows that pyroptosis can affect the development of tumors. The relationship between pyroptosis and tumors is diverse in different tissues and genetic backgrounds. In this review, we provide basic knowledge of pyroptosis, explain the relationship between pyroptosis and tumors, and focus on the significance of pyroptosis in tumor treatment. In addition, we further summarize the possibility of pyroptosis as a potential tumor treatment strategy and describe the side effects of radiotherapy and chemotherapy caused by pyroptosis. In brief, pyroptosis is a double-edged sword for tumors. The rational use of this dual effect will help us further explore the formation and development of tumors, and provide ideas for patients to develop new drugs based on pyroptosis.

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