Determination of Leishmania Species by PCR-RFLP in the Smear Samples Taken from the Lesions of Cutaneous Leishmaniasis Cases

2016 ◽  
Vol 50 (2) ◽  
pp. 300-306 ◽  
Author(s):  
Hatice ERTABAKLAR ◽  
Sema ERTUĞ ◽  
Serçin Özlem ÇALIŞKAN ◽  
Bülent BOZDOĞAN
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Species ◽  
Pcr Rflp

Identification of causative Leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP

Acta Tropica ◽  
2016 ◽  
Vol 158 ◽  
pp. 83-87 ◽  
Author(s):  
Yu Koarashi ◽  
Abraham G. Cáceres ◽  
Florencia Margarita Zúniga Saca ◽  
Elsa Elvira Palacios Flores ◽  
Adela Celis Trujillo ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Species ◽  
Pcr Rflp

scholarly journals Direct diagnosis of Leishmania species on serosity materials punctured from cutaneous leishmaniasis patients using PCR-RFLP

2011 ◽  
Vol 25 (1) ◽  
pp. 20-24 ◽  
Author(s):  
Homa Hajjaran ◽  
Farzaneh Vasigheh ◽  
Mehdi Mohebali ◽  
Sasan Rezaei ◽  
Setareh Mamishi ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Species ◽  
Pcr Rflp

scholarly journals Molecular Identification of Leishmania Species in an Outbreak of Re-Emerged Cutaneous Leishmaniasis in Southwestern Iran During 2015 - 2016

2021 ◽  
Vol 16 (3) ◽  
Author(s):  
Mehri Kargar ◽  
Homa Hajjaran ◽  
Javad Moazen ◽  
Mohammad Reza Shirzadi ◽  
Elham Kazemirad ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Health Center ◽  
Leishmania Major ◽  
Disease Incidence ◽  
Leishmania Species ◽  
Khuzestan Province ◽  
Pcr Rflp ◽  
Low Incidence ◽  
Southwestern Iran ◽  
Southwest Of Iran

Background: Dezful and its suburbs, as the second city in Khuzestan Province, southwest of Iran, has been an endemic area of cutaneous leishmaniasis (CL) with a low incidence rate since the last decades. However, the disease incidence has rapidly increased, and now is considered as a re-emerging parasitic disease in the area. Objectives: The aim of this study was to identify the most prevalent CL species in Dezful Region. Methods: A total of 196 microscopically confirmed slides from CL patients referred to Dezful Health Center were randomly collected in the period of 2015 - 2016. After DNA extraction from microscopically positive slides, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was carried out on 61 eligible specimens using ribosomal internal transcribed spacer 1 (ITS1) gene. The HaeIII restriction enzyme was used for the identification of species. Results: Samples were randomly collected from 196 acute CL cases, including 110 (56.2%) males and 86 (43.8%) females. Most infections were seen in the age range of 2 - 7 years (65/196, 33.1%). Totally, 60.1% of the cases had only one lesion, and half of the lesions appeared on hands. Furthermore, 162 (82.7%) cases were referred to Dezful Health Center in the cold seasons (autumn and winter). Results of PCR-RFLP on 61 eligible isolates showed that 60 (98.4%) isolates were Leishmania major, and only 1 (1.6%) isolate was Leishmania tropica. Conclusions: Our findings indicated that L. major is the main agent of re-emerged CL in Dezful and its suburbs, and the disease is a zoonosis.

scholarly journals The Investigation of the Association of Cutaneous Leishmaniasis in Biopsy Specimens of the Patients with Granulomatous Disease and Skin Cancer Using the Molecular Method

2020 ◽  
Author(s):  
Gülnaz ÇULHA ◽  
Asena Çiğdem DOĞRAMACI ◽  
Sibel HAKVERDİ ◽  
İlke Evrim SEÇİNTİ ◽  
Özkan ASLANTAŞ ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Cutaneous Leishmaniasis ◽  
Leishmania Species ◽  
Pcr Analysis ◽  
Biopsy Specimens ◽  
Skin Cancers ◽  
Pcr Rflp ◽  
Granulomatous Dermatitis ◽  
Tissue Specimens ◽  
Granulomatous Diseases

Background: Clinically, cutaneous leishmaniasis (CL) can be confused with granulomatous diseases and skin cancers, and it may lead to erroneous diagnosis and treatment. Diagnosis based and histopathology can have some difficulties due to low number of parasites, especially in chronic CL cases. We aimed to emphasize the necessity of considering CL in the differential diagnosis for cases of granulomatous diseases and basal cell carcinoma, particularly in areas where CL is endemic. Methods:  One hundred and seven paraffin-embedded tissue biopsy specimens were selected from the archive, as of 2002, of Pathology Department, School of Medicine, University of Hatay Mustafa Kemal in Hatay, Turkey. After DNA isolation, performed with the samples were used for PCR analysis with specific 13A, 13B primers targeting kinetoplastid DNA (kDNA) found in all Leishmania species. Another PCR was performed with LITSR and L5.8S primers targeting ITS-1 internal-transcribed-spacer-1 (ITS-1) region to subtype positive samples. Then these samples were further analyzed for subtyping with PCR-RFLP using HaeIII enzyme (BsuRI). Results: Ten out of 107 tissue specimens were positive via kDNA-PCR. Lupus vulgaris, sarcoidosis, skin lymphoma and Leishmania cutis appeared in 9 out of 10 positive specimens. One of the cases presented with a mass on the cheek and was pre-diagnosed with hemangioma, but leishmaniasis did not appear. All of 10 specimens were diagnosed as granulomatous dermatitis. Two out of 10 samples, found positive with kDNA-PCR, were analyzed with ITS-1-PCR and identified as L. infantum/donovani after RFLP. Conclusion: Molecular methods should be utilized in the differential diagnosis of CL to eliminate false diagnoses of granulomatous diseases and skin cancers.

scholarly journals ITS1 PCR-RFLP Diagnosis and Characterization ofLeishmaniain Clinical Samples and Strains from Cases of Human Cutaneous Leishmaniasis in States of the Mexican Southeast

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Amalia Monroy-Ostria ◽  
Abedelmajeed Nasereddin ◽  
Victor M. Monteon ◽  
Carmen Guzmán-Bracho ◽  
Charles L. Jaffe
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Low Cost ◽  
Clinical Samples ◽  
Mucocutaneous Leishmaniasis ◽  
Pcr Rflp ◽  
Different Strains ◽  
Mixed Pattern ◽  
Short Time ◽  
Rflp Assay

American cutaneous leishmaniasis includes a spectrum of clinical forms localized cutaneous, diffuse cutaneous, and mucocutaneous leishmaniasis which can be caused by different strains ofLeishmaniabelonging to theL. mexicanaorL. braziliensiscomplexes which may coexist in the same endemic area. We evaluated the PCR-RFLP assay of the ITS1 genes for direct identification ofLeishmaniaspecies in 163 clinical samples and 21 Mexican isolates ofLeishmania. In relation to the Mexican isolates ofLeishmania52% displayed a pattern similar to theL. (L.) mexicana, 5% showed a mixed pattern compatible withL. (L.) mexicanaandL. (V.) braziliensis, eight withL. (L.) amazonensisandL. (L.) mexicana, and one toL. (V.) braziliensis. Most of the clinical samples, 109/116 (94%), gave a pattern similar to that of theL. mexicana, two clinical samples gave similar patterns to that ofLeishmaniabraziliensis, and 5 samples gave patterns that suggest a coinfection ofL. (L.) mexicanaandL. (V.) braziliensisorL. (L.) mexicanaandL. (L.) amazonensis. The ITS1 PCR-RFLP assay is a multipurpose tool for diagnosis ofLeishmaniafrom clinical samples and enables determination of the infecting species of New WorldLeishmaniain the field in relatively short time and low cost.

DETERMINATION OF P53 GENE CODON 72 POLYMORPHISMS IN PATIENTS WITH GASTRIC CANCER

2013 ◽  
pp. 11-17
Author(s):  
Thi Tuy Ha Nguyen ◽  
Thi Minh Thi Ha
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
P53 Gene ◽  
Diffuse Gastric Cancer ◽  
Codon 72 ◽  
Cancer Group ◽  
Pcr Rflp ◽  
Gastric Cancer Patients ◽  
The Relationship

Background: The role of p53 gene in the gastric cancer is still controversial. This study is aimed at determining the rate of the p53 gene codon 72 polymorphisms in gastric cancer patients and evaluating the relationship between these polymorphisms and endoscopic and histopathological features of gastric cancer. Patients and methods: Sixty eight patients with gastric cancer (cases) and one hundred and thirty six patients without gastric cancer (controls) were enrolled. p53 gene codon 72 polymorphisms were determined by PCR-RFLP technique with DNA extracted from samples of gastric tissue. Results: In the group of gastric cancer, Arginine/Argnine, Arginine/Proline and Proline/Proline genotypes were found in 29.4%, 42.7% and 27.9%, respectively. The differences of rates were not statistically significant between cases and controls (p > 0,05). In males, the Proline/Proline genotype was found in 38.1% in patients with gastric cancer and more frequent in patients without gastric cancer (15.7%, p = 0,01). An analysis of ROC curve showed that the cut-off was the age of 52 in the Proline/Proline genotype, but it was 65 years old in the Arginine/Proline genotype. The Proline/Proline genotype was found in 41.9% in Borrmann III/IV gastric cancer, this rate was higher than Borrmann I/II gastric cancer (16.2%, p = 0.037) and also higher than controls (18.4%, p = 0,01). The rate of Proline/Proline genotype was 41.7% in the diffuse gastric cancer, it was higher than in controls (p = 0,023). Conclusion: No significative difference of rate was found in genotypes between gastric cancer group and controls. However, there was the relationship between Proline/Proline genotype and gastric cancer in males, Borrmann types of gastric cancer, the diffuse gastric cancer. Key words: polymorphism, codon 72, p53 gene, PCR - RFLP, gastric cancer.

scholarly journals The Molecular Bases Study of the Inherited Diseases for the Health Maintenance of the Beef Cattle

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 678
Author(s):  
Elena Konovalova ◽  
Olga Romanenkova ◽  
Olga Kostyunina ◽  
Elena Gladyr
Keyword(s):  
Dna Analysis ◽  
Genetic Defects ◽  
Health Maintenance ◽  
Angus Cattle ◽  
Hereford Cattle ◽  
Pcr Rflp ◽  
Cattle Herds ◽  
Molecular Bases ◽  
Further Development

The article highlighted the problem of meat cattle genetic defects. The aim was the development of DNA tests for some genetic defects diagnostics, the determination of the animal carriers and their frequencies tracking in time. The 1490 DNA samples from the Aberdeen Angus (n = 701), Hereford (n = 385), Simmental (n = 286) and Belgian Blue (n = 118) cattle have been genotyped on the genetic defects by newly created and earlier developed DNA tests based on AS-PCR and PCR-RFLP methods. The Aberdeen Angus cattle genotyping has revealed 2.38 ± 0.31% AMC-cows and 1.67 ± 0.19 % AMC-bulls, 0.65 ± 0.07% DDC-cows and 0.90 ± 0.10% DDC-bulls. The single animals among the Hereford cattle were carriers of MSUD and CWH (on 0.27 ± 0.05%), ICM and HY (on 0.16 ± 0.03%). The Simmental cattle were free from OS. All Belgian Blue livestock were M1- and 0.84%-CMD1-carriers. The different ages Aberdeen Angus cattle genotyping has shown the tendency of the AMC- and DDC frequencies to increase in the later generations. The statistically significant increase of DDC of 1.17% in the cows’ population born in 2019 compared to those born in 2015 allows concluding the further development of the DNA analysis-based measures preventing the manifestation of the genetic anomalies in meat cattle herds is necessary.

The Role of CD1a expression in the diagnosis of cutaneous leishmaniasis, its relationship with Leishmania species and clinicopathological features

2021 ◽  
Author(s):  
Yasemin Yuyucu Karabulut ◽  
Funda KuŞ Bozkurt ◽  
Ümit Türsen ◽  
Gül Bayram ◽  
Gülhan Örekeci Temel ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Species ◽  
Clinicopathological Features
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