scholarly journals ITS1 PCR-RFLP Diagnosis and Characterization ofLeishmaniain Clinical Samples and Strains from Cases of Human Cutaneous Leishmaniasis in States of the Mexican Southeast

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Amalia Monroy-Ostria ◽  
Abedelmajeed Nasereddin ◽  
Victor M. Monteon ◽  
Carmen Guzmán-Bracho ◽  
Charles L. Jaffe
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Low Cost ◽  
Clinical Samples ◽  
Mucocutaneous Leishmaniasis ◽  
Pcr Rflp ◽  
Different Strains ◽  
Mixed Pattern ◽  
Short Time ◽  
Rflp Assay

American cutaneous leishmaniasis includes a spectrum of clinical forms localized cutaneous, diffuse cutaneous, and mucocutaneous leishmaniasis which can be caused by different strains ofLeishmaniabelonging to theL. mexicanaorL. braziliensiscomplexes which may coexist in the same endemic area. We evaluated the PCR-RFLP assay of the ITS1 genes for direct identification ofLeishmaniaspecies in 163 clinical samples and 21 Mexican isolates ofLeishmania. In relation to the Mexican isolates ofLeishmania52% displayed a pattern similar to theL. (L.) mexicana, 5% showed a mixed pattern compatible withL. (L.) mexicanaandL. (V.) braziliensis, eight withL. (L.) amazonensisandL. (L.) mexicana, and one toL. (V.) braziliensis. Most of the clinical samples, 109/116 (94%), gave a pattern similar to that of theL. mexicana, two clinical samples gave similar patterns to that ofLeishmaniabraziliensis, and 5 samples gave patterns that suggest a coinfection ofL. (L.) mexicanaandL. (V.) braziliensisorL. (L.) mexicanaandL. (L.) amazonensis. The ITS1 PCR-RFLP assay is a multipurpose tool for diagnosis ofLeishmaniafrom clinical samples and enables determination of the infecting species of New WorldLeishmaniain the field in relatively short time and low cost.

scholarly journals Molecular Epidemiology of Mycoplasma conjunctivae in Caprinae: Transmission across Species in Natural Outbreaks

2003 ◽  
Vol 69 (4) ◽  
pp. 1913-1919 ◽  
Author(s):  
Luc Belloy ◽  
Martin Janovsky ◽  
Edy M. Vilei ◽  
Paola Pilo ◽  
Marco Giacometti ◽  
...  
Keyword(s):  
Variable Domain ◽  
Clinical Samples ◽  
Ocular Infection ◽  
European Alps ◽  
Sequence Determination ◽  
Molecular Method ◽  
Infectious Keratoconjunctivitis ◽  
Mycoplasma Conjunctivae ◽  
Different Strains

ABSTRACT Mycoplasma conjunctivae is the etiological agent of infectious keratoconjunctivitis, a highly contagious ocular infection that affects both domestic and wild Caprinae species in the European Alps. In order to study the transmission and spread of M. conjunctivae across domestic and wild Caprinae populations, we developed a molecular method for subtyping and identifying strains of M. conjunctivae. This method is based on DNA sequence determination of a variable domain within the gene lppS, a gene that encodes an antigenic lipoprotein of M. conjunctivae. This domain of lppS shows variations among different strains but remains constant upon generations of individual strains on growth medium and thus allows identification of individual strains and estimation of their phylogenetic intercorrelations. The variable domain of lppS is amplified by PCR using primers that match conserved sequences of lppS flanking it. Sequence analysis of the amplified fragment enables fine subtyping of M. conjunctivae strains. The method is applicable both to isolated strains and to clinical samples directly without requiring the cultivation of the strain. Using this method, we show that M. conjunctivae was transmitted between domestic and wild animals that were grazing in proximate pastures. Certain animals also presented infections with two different strains simultaneously.

Recent Electrochemical Assays on Cephalosporins

2020 ◽  
Vol 16 (4) ◽  
pp. 337-349
Author(s):  
Leyla Karadurmus ◽  
Kaan Eşme ◽  
Nurgul K. Bakirhan ◽  
Sibel A. Ozkan
Keyword(s):  
Low Cost ◽  
Linear Sweep Voltammetry ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Electroanalytical Methods ◽  
Antibiotic Drugs ◽  
Short Time ◽  
Advanced Generation ◽  
Antibacterial Spectrum

: Antibiotics are an important class among drugs because they are a significant agent to deal with infections. Cephalosporins are a very important group of antibiotics in the β-lactam class. The cephalosporins are semisynthetic antibiotics derived from products of the fungus Cephalosporium. Cephalosporins are classified as first, second, third, fourth, and advanced generation, based largely on their antibacterial spectrum and stability to β-lactamases. Electrochemical methods have been used for the determination of cephalosporin just as used in the determination of many antibiotic drugs. Electroanalytical methods present generally high sensitivity, low cost, low requirements, ease of preparation of the samples in a very short time, and a short analysis time. The most commonly used types are cyclic voltammetry, differential pulse voltammetry, square wave voltammetry and linear sweep voltammetry. The aim of this review is to evaluate the advantages and uses of electroanalytical methods used in the determination of cephalosporins. In addition, current applications of the methods to the pharmaceutical analysis of cephalosporins will also be summarized in a table.

Determination of Leishmania Species by PCR-RFLP in the Smear Samples Taken from the Lesions of Cutaneous Leishmaniasis Cases

2016 ◽  
Vol 50 (2) ◽  
pp. 300-306 ◽  
Author(s):  
Hatice ERTABAKLAR ◽  
Sema ERTUĞ ◽  
Serçin Özlem ÇALIŞKAN ◽  
Bülent BOZDOĞAN
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Species ◽  
Pcr Rflp

scholarly journals Determination of Haptoglobin in Bovine Serum using Polyclonal and Monoclonal Anti-human Haptoglobin Antibodies

2010 ◽  
Vol 79 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Paulina Jawor ◽  
Tadeusz Stefaniak ◽  
Iwona Kątnik-Prastowska
Keyword(s):  
Monoclonal Antibody ◽  
Detection Limit ◽  
Bovine Serum ◽  
Solid Phase ◽  
Low Cost ◽  
Reference Method ◽  
Serum Samples ◽  
Incubation Periods ◽  
Short Time

Two ELISA procedures to determine haptoglobin (Hp) in bovine serum were developed. Equine haemoglobin was used as the solid phase. Self-developed goat polyclonal antibody (variant I) and monoclonal antibody (variant II) raised against human Hp were used. The results were compared with the guaiacol method. High correlation was found (r = 0.96 and r = 0.90, respectively) based on the results of 548 bovine serum samples, of which 357 were from clinically healthy cows and 191 from cows and calves monitored during treatment for the most common diseases. The Hp detection limit of ELISA using polyclonal Ab was 0.1 mg/l and using MoAb 0.21 mg/l. The addition of 2% PEG 6000 at the antibody-binding steps enabled major shortening of the incubation periods. The relatively short time, low cost of reagents, and high correlation with the reference method support the use of these ELISA variants in bovine diagnostics.

scholarly journals Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP

2018 ◽  
pp. 162-171 ◽  
Author(s):  
Aref Teimouri ◽  
Mehdi Mohebali ◽  
Elham Kazemirad ◽  
Homa Hajjaran
Keyword(s):  
Visceral Leishmaniasis ◽  
Cutaneous Leishmaniasis ◽  
Rflp Analysis ◽  
Canine Visceral Leishmaniasis ◽  
Species Comparison ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Leishmania Spp ◽  
Human Cutaneous Leishmaniasis ◽  
Rflp Assay

Background: Leishmaniasis is a major medical health problem and distributes in nearly half of 31 provinces of Iran. We aimed to identify cutaneous and visceral Leishmania spp. isolated from infected humans and domestic dogs in various regions of Iran, 2010‒2013. Methods: DNA was extracted from 108 lesion exudate samples of suspected patients to cutaneous leishmaniasis and nine liver and spleen aspirates of infected dogs cultured in RPMI-1640 and amplified using partial sequence of ITS1 gene. The PCR amplicons were digested using HaeIII endonuclease enzyme and used in restriction fragment length polymorphism (RFLP) assay. Then, 48 amplicons representing various hosts were sequenced and compared to se­quences from GenBank databases using BLAST. Results: PCR-RFLP analysis showed that 60 and 48 CL patients were infected by Leishmania tropica and L. major, respectively. From nine canine visceral leishmaniasis (CVL) isolates, eight isolates were identified as L. infantum and one as L. tropica. The greatest similarity of 95.7% in ITS1 region was seen between L. infantum and L. major. Furthermore, the lowest similarity with 65.7% was seen between L. tropica and L. major. Intra-species comparison of ITS1 region in L. infantum, L. major and L. tropica isolates were showed 100%, 98.2% and 72.4 % similarities, respectively. Conclusion: PCR-RFLP based on ITS1 region is an appropriate method to distinguish three Leishmania spp. of L. major, L. tropica, and L. infantum. In intra-species comparison of ITS1 region, genotypic variations showed that L. tropica isolates were more heterogeneous than L. major and L. infantum isolates.

PCR-RFLP Detection od PAI-2 Gene Variants: Prevelence in Ethnic Groups and Disease Relationship in patients Undergoing corony Angiography

1997 ◽  
Vol 77 (05) ◽  
pp. 0955-0958 ◽  
Author(s):  
Carole A Foy ◽  
Peter J Grant
Keyword(s):  
Gene Variants ◽  
Genotype Distribution ◽  
Pima Indians ◽  
Significant Difference ◽  
Pcr Rflp ◽  
History Of ◽  
Caucasian Patients ◽  
Clinical Conditions ◽  
Rflp Assay ◽  
Coronary Atheroma

SummaryPAI-2 is a fibrinolytic inhibitor produced predominantly by monocytes. Most PAI-2 is intracellular making study in clinical conditions difficult. Abnormalities in production may be associated with inflammation and fibrinolysis at sites of tissue damage such as the atherosclerotic plaque.PAI-2 gene variants have been described: variant A consists of Asn120, Asn404 and Ser413 and variant B consists of Asp120, Lys404 and Cys413. We designed a PCR-RFLP assay using primers spanning the region containing Asn/Lys404 and Ser/Cys413. Variant B contains an Mwol restriction site. We analysed 302 Pima Indians and 286 healthy Caucasian volunteers. To investigate relationships between genotype and vascular disease we analysed 333 Caucasian patients undergoing coronary angiography.Gene variant B was more common in the Pimas than in Caucasians (p <0.0001). There was no significant difference in genotype distribution between the volunteers and patients. In the patients there was no association between genotype and either a history of MI or extent of coronary atheroma.

Low-Cost Hardware Implementation of Human Blood Identification Device Using Feed-Forward Artificial Neural Network on FPGA

2018 ◽  
Author(s):  
Rizki Eka Putri ◽  
Denny Darlis
Keyword(s):  
Neural Network ◽  
Artificial Neural Network ◽  
Low Cost ◽  
Image Resolution ◽  
Blood Type ◽  
Feed Forward ◽  
Testing Facility ◽  
System Input ◽  
Artificial Neural ◽  
Short Time

This article was under review for ICELTICS 2018 -- In the medical world there is still service dissatisfaction caused by lack of blood type testing facility. If the number of tested blood arise, a lot of problems will occur so that electronic devices are needed to determine the blood type accurately and in short time. In this research we implemented an Artificial Neural Network on Xilinx Spartan 3S1000 Field Programable Gate Array using XSA-3S Board to identify the blood type. This research uses blood sample image as system input. VHSIC Hardware Discription Language is the language to describe the algorithm. The algorithm used is feed-forward propagation of backpropagation neural network. There are 3 layers used in design, they are input, hidden1, and output. At hidden1layer has two neurons. In this study the accuracy of detection obtained are 92%, 92%, 92%, 90% and 86% for 32x32, 48x48, 64x64, 80x80, and 96x96 pixel blood image resolution, respectively.

Robust Test Method of TSOP PEMs for Space Application

2019 ◽  
Author(s):  
Yasunobu Iwai ◽  
Koichi Shinozaki ◽  
Daiki Tanaka
Keyword(s):  
Evaluation Method ◽  
Low Cost ◽  
Reliability Evaluation ◽  
Test Method ◽  
Thermal Shock Test ◽  
Space Application ◽  
Space Applications ◽  
Limit Test ◽  
Long Time ◽  
Short Time

Abstract Compared with space parts, consumer parts are highly functional, low cost, compact and lightweight. Therefore, their increased usage in space applications is expected. Prior testing and evaluation on space applicability are necessary because consumer parts do not have quality guarantees for space application [1]. However, in the conventional reliability evaluation method, the test takes a long time, and the problem is that the robustness of the target sample can’t be evaluated in a short time. In this report, we apply to the latest TSOP PEM (Thin Small Outline Package Plastic Encapsulated Microcircuit) an evaluation method that combines preconditioning and HALT (Highly Accelerated Limit Test), which is a test method that causes failures in a short time under very severe environmental conditions. We show that this method can evaluate the robustness of TSOP PEMs including solder connections in a short time. In addition, the validity of this evaluation method for TSOP PEM is shown by comparing with the evaluation results of thermal shock test and life test, which are conventional reliability evaluation methods.

Sign in / Sign up

Export Citation Format

Share Document