scholarly journals Early-Arbuscular Mycorrhizal Fungi-Application Improved Physiological Performances of Sugarcane Seedling and Further Growth in the Dry Land

2017 ◽  
Vol 9 (4) ◽  
pp. 95 ◽  
Author(s):  
Wawan Sulistiono ◽  
Taryono Taryono ◽  
Prapto Yudono ◽  
Irham Irham
Keyword(s):  
Mycorrhizal Fungi ◽  
Physiological Stress ◽  
Intercellular Co2 Concentration ◽  
Arbuscular Mycorrhizal ◽  
Co2 Concentration ◽  
Dry Land ◽  
Randomized Design ◽  
Net Assimilation ◽  
And Control ◽  
Infection Percentage

The Indonesian sugarcane production in the dryland was not optimal due to physiological stress. The research to improve their physiological characteristics by arbuscular mycorrhizal application was conducted at inceptisol soil from April 2014 to February 2015, using factorial completely randomized design. The first factor was clones PS864, Kidangkencana, PS881, Bululawang, and VMC, whereas the second factor was timing of arbuscular mycorrhizal inoculum applications i.e.: in the field, nursery, and control. The results show that the mycorrhizal application in the nursery significantly increased the infection percentage (41.3%) at the age of 1 week after transplanting to the field. There are differences in physiological and growth performance of clones by the effect of timing of mycorrhizal application. Bululawang with mycorrhizal application in the nursery stage tended to have better physiological performance such as chlorophylls a, b, and total chlorophyll, photosynthesis rate, intercellular CO2 concentration, leaf area, as well as the weight of stem biomass. Mycorrhizal application in the nursery stage showed the highest increase of net assimilation rate on the PS864 and, BL clones, and the highest crop growth rate on the PS864, KK and BL clones. The mycorrizal inoculum application in the nursery increased the biomass weight of stem (11-61.1%). Infection percentage of mycorrhiza was positively correlated with the biomass weight with r2 values of 0.54.

scholarly journals Enhancement of clover growth by inoculation of P-solubilizing fungi and arbuscular mycorrhizal fungi

2010 ◽  
Vol 82 (3) ◽  
pp. 771-777 ◽  
Author(s):  
Edson L. Souchie ◽  
Rosario Azcón ◽  
Jose M. Barea ◽  
Eliane M.R. Silva ◽  
Orivaldo J. Saggin-Júnior
Keyword(s):  
Growth Rate ◽  
Aspergillus Niger ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Trifolium Pratense ◽  
Arbuscular Mycorrhizal ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Environment Chamber ◽  
And Control

This study evaluated the synergism between several P-solubilizing fungi isolates and arbuscular mycorrhizal fungi to improve clover ( Trifolium pratense) growth in the presence of Araxá apatite. Clover was sown directly in plastic pots with 300g of sterilized washed sand, vermiculite and sepiolite 1:1:1 (v:v:v) as substrate, and grown in a controlled environment chamber. The substrate was fertilized with 3 g L-1 of Araxá apatite. A completely randomized design, in 8×2 factorial scheme (eight P-solubilizing fungi treatments with or without arbuscular mycorrhizal fungi)and four replicates were used. The P-solubilizing fungi treatments consisted of five Brazilian P-solubilizing fungi isolates (PSF 7, 9, 20, 21 and 22), two Spanish isolates ( Aspergillus niger and the yeast Yarowia lipolytica) and control (non-inoculated treatment). The greatest clover growth rate was recorded when Aspergillus niger and PSF 21 were co-inoculated with arbuscular mycorrhizal fungi. Aspergillus niger, PSF 7 and PSF 21 were the most effective isolates on increasing clover growth in the presence of arbuscular mycorrhizal fungi. Greater mycorrhizal colonization resulted in greater clover growth rate in most PSF treatments. PSF 7 was the best isolate to improve the establishment of mycorrhizal and rhizobia symbiosis.

scholarly journals Evaluation of Woven Agrotextiles in the Development of Hemp (Cannabis sativa L.) in Greenhouse

2020 ◽  
Vol 77 (1) ◽  
pp. 53
Author(s):  
Antigolena FOLINA ◽  
Ioannis ROUSSIS ◽  
Varvara KOUNELI ◽  
Ioanna KAKABOUKI ◽  
Stella KARIDOGIANNI ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Soil Cover ◽  
Cannabis Sativa ◽  
Higher Plants ◽  
Arbuscular Mycorrhizal ◽  
Randomized Design ◽  
Covered Area ◽  
Absolute Growth ◽  
And Control ◽  
Cover Material

The effect of the reflection by a white agrotextile, in hemp cultivation, was evaluated on a greenhouse experiment during 2019. The experiments were laid out in a completely randomized design with two treatments (soil cover and control). The results indicated that the average temperature in the covered area section was 28.5ºC while in the uncovered area was 25.12ºC, during the experiment. Concerning height, higher plants up to 20 cm, were recorded on the covered area than the uncovered one. The highest specific leaf area (SLA) (58.39 cm2 g-1) was found on the covered area at 60 days after transplanting (DAT) compared to the uncovered area where a value of 50.4 cm2 g-1 was recorded. The absolute growth rate was higher during 20-60 DAT comparatively to control. The highest rate was observed about 50-60 DAT where it reached the value of 3.34 g day-1. Concerning root system, arbuscular mycorrhizal fungi (AMF) was only affected by the cover material. The number of inflorescences was not found to be affected by the cover material but the bud length and the cannabidiol (CBD) content were affected by the soil cover. The cannabinoids (CBD) content was 28.8% higher on covered area.

scholarly journals The Effectiveness of Endomycorrhiza Species on the Growth of Barangan Banana Seedlings

2019 ◽  
Vol 1 (4) ◽  
pp. 70-76
Author(s):  
Asmah Indrawati ◽  
Suswati Suswati
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Seedling Growth ◽  
Mycorrhizal Fungi ◽  
Leaf Growth ◽  
Arbuscular Mycorrhizal ◽  
Sand Mixture ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Control

Utilization of various species of arbuscular mycorrhizal fungi in Barangan banana nurseries has been carried out in a wire house. The aim of the study was to obtain data on the effectiveness of Barangan banana seedling growth after various FMA species were applied. This research was conducted with a Completely Randomized Design with mycorrhizal (A) treatment consisting of 4 levels, namely: A0. Without mycorrhizae, A1. Glomus type 1; A2. Acaulospora type 4 and A3. Glomus fasciculatum (FMA isolate collection of Prof. Dr. Ir. Eti Farda Husin, MS) with 5 replications. A total of 10 g of FMA inoculants were separately put into the acclimatization media of the Barangan plantlet in the form of a sterilized charcoal and sand mixture (2: 1). during acclimatization. The results of this study indicate that seedling growth is better in the treatment of FMA applications than in controls. Acaulospora type-4 FMA isolates; Glomus type-1 and G. fasciculatum can increase the height of Barangan banana seedlings at a rate of: 3.117%; 2,831% and 2,328% while the rate of leaf growth is better in Glomus type-1 applications (1,865%), G. fasciculatum (1,709%) and Acaulospora type 4 (1,412 and control (1,174%).

scholarly journals First Insights into the Effect of Mycorrhizae on the Expression of Pathogen Effectors during the Infection of Grapevine with Plasmopara viticola

2021 ◽  
Vol 13 (3) ◽  
pp. 1226
Author(s):  
Ana Cruz-Silva ◽  
Andreia Figueiredo ◽  
Mónica Sebastiana
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Plasmopara Viticola ◽  
Vitis Vinifera L ◽  
Wine Production ◽  
Grapevine Downy Mildew ◽  
Pathogen Effectors ◽  
Rxlr Effectors ◽  
Mycorrhizal Inoculation ◽  
And Control

Grapevine (Vitis vinifera L.), widely used for berry and wine production, is highly susceptible to the pathogenic oomycete Plasmopara viticola, the etiological agent of grapevine downy mildew disease. The method commonly used to prevent and control P. viticola infection relies on multiple applications of chemical fungicides. However, with European Union goals to lower the usage of such chemicals in viticulture there is a need to develop new and more sustainable strategies. The use of beneficial microorganisms with biocontrol capabilities, such as the arbuscular mycorrhizal fungi (AMF), has been pointed out as a viable alternative. With this study, we intended to investigate the effect of AMF colonization on the expression of P. viticola effectors during infection of grapevine. Grapevine plants were inoculated with the AMF Rhizophagus irregularis and, after mycorrhizae development, plants were infected with P. viticola. The expression of P. viticola RxLR effectors was analyzed by real-time PCR (qPCR) during the first hours of interaction. Results show that pre-mycorrhizal inoculation of grapevine alters the expression of several P. viticola effectors; namely, PvRxLR28, which presented decreased expression in mycorrhizal plants at the two time points post-infection tested. These results suggest that the pre-inoculation of grapevine with AMF could interfere with the pathogen’s ability to infect grapevine by modulation of pathogenicity effectors expression, supporting the hypothesis that AMF can be used to increase plant resistance to pathogens and promote more sustainable agriculture practices, particularly in viticulture.

scholarly journals EFFECT OF ARBUSCULAR MYCORRHIZAL FUNGI BIOFERTILIZER ON THE GROWTH OF CASHEW SEEDLING

2020 ◽  
Vol 17 (4) ◽  
pp. 150
Author(s):  
O. TRISILAWATI
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Phosphatase Activity ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Anacardium Occidentale ◽  
Glomus Etunicatum ◽  
Gigaspora Margarita ◽  
Randomized Design ◽  
Phosphorus And Potassium ◽  
Glomus Sp

<p>ABSTRACT</p><p>The effects of several arbuscular mycorrhizal fungi (AMF) on thegrowth, nutrient uptake (nitrogen, phosphorus, and potassium), and acidphosphate activity of two promising numbers of Anacardium occidentaleseedling were evaluated. The experiment was conducted in the greenhouse of Indonesian Spices and Medicinal Crops Research Institute(BALITTRO) in 2002 for six months on a randomized design with twofactors and four replicates. First factor was isolate (six isolates of AMFand one control) consisting of : 1) control; 2) Glomus aggregatum; 3)Glomus etunicatum; 4) Mycofer; 5) Glomus sp.; 6) a mixture of Glomussp1, Glomus sp2, Glomus sp3, Glomus sp4, Glomus etunicatum,Gigaspora margarita, Gigaspora sp., and Enthropospora sp., and 7)Gigaspora sp. The second factor was two cashew promising numbers :Asembagus and Wonogiri. The results showed that AMF inoculationsignificantly affected the growth of cashew. Mycofer and mixed AMFwere more effective to Wonogiri promising number, while for Asembaguspromising number inoculation of mycofer was more effective. Inoculationwith mycofer to Asembagus promising number increased the uptake of Pand K nutrients by 65 and 53% while inoculation with mycofer and mixedAMF to Wonogiri promising number increased the uptake of N, P and Knutrients by 55, 38, and 17%, and by 18, 31, and 17%. Moreover, theAMF inoculation resulted in higher phosphatase activity. In mycorrhizalAsembagus promising number infected by mixed AMF, the increment ofphosphatase activity was 136.5%, whether in Wonogiri promising numberinfected by mycofer, the increment of phosphatase activity was 80% thancontrol.</p><p>Key words: Anacardium occidentale, promising number, growth,phosphatase activity</p><p>ABSTRAK</p><p>Pengaruh Pupuk Hayati Fungi Mikoriza Arbuskula(FMA) terhadap Pertumbuhan Benih Jambu Mete</p><p>Penelitian ini bertujuan untuk mengetahui pengaruh beberapa jenisfungi mikoriza arbuskula (FMA) terhadap pertumbuhan, serapan hara danaktivitas enzim fosfatase dari dua nomor harapan benih jambu mete(Anacardium occidentale). Penelitian dilakukan di rumah kaca Balittropada tahun 2002 selama 6 bulan, menggunakan rancangan acak yangterdiri dari dua faktor dan diulang empat kali. Faktor pertama adalahisolate (6 jenis isolat FMA dan satu kontrol) yaitu: 1). kontrol; 2). Glomusaggregatum; 3) Glomus etunicatum; 4). Mycofer; 5). Glomus sp.; 6).campuran dari Glomus sp1, Glomus sp2, Glomus sp3, Glomus sp4, Glomusetunicatum, Gigaspora margarita, Gigaspora sp., Enthropospora sp., dan7). Gigaspora sp. Faktor kedua adalah nomor harapan jambu mete, yaituAsembagus dan Wonogiri. Hasil penelitian mendapatkan bahwa inokulasiFMA berpengaruh nyata terhadap pertumbuhan jambu mete. Mycofer dancampuran FMA lebih efektif berpengaruh terhadap nomor harapanWonogiri, sedangkan mycofer lebih efektif berpengaruh terhadap nomorharapan Asembagus. Serapan hara P dan K pada nomor harapanAsembagus yang diinokulasi mycofer meningkat sebesar 65 dan 53%,sedangkan nomor harapan Wonogiri yang diinokulasi mycofer dancampuran FMA, serapan hara N, P, dan K meningkat masing-masingsebesar 55; 38; dan 17%, dan 18; 31; dan 17%. Selain itu, inokulasi FMAdapat meningkatkan aktivitas fosfatase akar jambu mete. Peningkatanaktivitas fosfatase akar jambu mete nomor harapan Asembagus yangterinfeksi oleh campuran FMA sebesar 136,5%, sedangkan pada nomorharapan Wonogiri yang terinfeksi mycofer, peningkatnnya sebesar 80%dibandingkan kontrol.</p><p>Kata kunci: Anacardium occidentale, nomor harapan, pertumbuhan,aktivitas fosfatase</p>

scholarly journals PENGGUNAAN ASAM HUMIK DALAM KULTUR TRAPPING CENDAWAN MIKORIZA ARBUSKULA DARI EKOSISTEM DENGAN SALINITAS TINGGI

2017 ◽  
Vol 9 (2) ◽  
pp. 124-129
Author(s):  
Delvian Delvian
Keyword(s):  
Humic Acid ◽  
Host Plant ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Spore Production ◽  
Control Treatment ◽  
Salinity Levels ◽  
And Control ◽  
Microbe Population ◽  
Pot Culture

It has been reported that humic acid can increase plant growth and benefecial soil microbe population.   A glasshouse experiment was conducted to study the effect of humic acid on growth of host plant and sporulation of arbuscular mycorrhizal fungi under salt stress in trap pot culture. Trap cultures were maintenance at various salinity levels as according to field salinity.  Half of the pots received 2.5% humic acid and control treatment received no humic acid. Result of research indicate that humic acid  can increase growth of host plant and mycorrhizae sporulation at all salinity levels. Acaulospora  is highest   its spore production   followed by of Glomus and Gigaspora .

scholarly journals PENGARUH PEMBERIAN CENDAWAN MIKORIZA ARBUSCULAR (CMA) DAN DOSIS PUPUK P TERHADAP PERTUMBUHAN DAN PRODUKSI OKRA (Abelmoschus esculentus L)

2021 ◽  
Vol 3 (2) ◽  
pp. 601-612
Author(s):  
Roni Novianto ◽  
◽  
Sri Hartatik ◽  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Plant Height ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Fruit Weight ◽  
Plant Weight ◽  
Randomized Design ◽  
Two Factors ◽  
P Fertilizer ◽  
Number Of Leaves

Okra is a vegetable crop that has a high selling value and the production of okra is more exported than sold domestically. The demand for okra from year to year continues to increase from various countries, especially Japan, which imports the most okra from Indonesia. Fertilization of phosphorus (P) fertilizer to fulfill nutrients and provision of arbuscular mycorrhizal fungi (CMA) for more efficient and maximum absorption by plants. Phosphorus (P) fertilizer can increase the production and quality of okra. The aim of this study was to determine the combination of treatment of arbuscular mycorrhizal fungi and which dosage of P fertilizer was best to increase the yield of okra production. The design used was a completely randomized design (CRD) with factorial treatment consisting of two factors. The first factor is the dose of arbucular mycorrhizal fungi (CMA) consisting of 4 levels, namely (0; 160; 320; 480) and the second factor is the dose of P fertilizer (75; 100; 125; 150). Parameters observed were plant height, number of leaves per plant, volume of roots, number of fruits per plant, weight of fresh fruit per plant and weight of plant dry corpse. Based on the analysis of variance, it showed that the interaction was significantly different on the variables of root volume, plant height, number of fruits and fruit weight. Based on research, the CMA dose of 320 kg / ha and the dose of 125 kg / ha gave the highest production results.

scholarly journals Arbuscular mycorrhizal fungi in soil aggregates from fields of "murundus" converted to agriculture

2015 ◽  
Vol 50 (4) ◽  
pp. 313-321 ◽  
Author(s):  
Marco Aurélio Carbone Carneiro ◽  
Dorotéia Alves Ferreira ◽  
Edicarlos Damacena de Souza ◽  
Helder Barbosa Paulino ◽  
Orivaldo José Saggin Junior ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Soil Aggregates ◽  
Geometric Mean ◽  
Arbuscular Mycorrhizal ◽  
Spore Density ◽  
Extraradical Mycelium ◽  
Randomized Design ◽  
Conversion Time ◽  
Completely Randomized Design

The objective of this work was to evaluate the spore density and diversity of arbuscular mycorrhizal fungi (AMF) in soil aggregates from fields of "murundus" (large mounds of soil) in areas converted and not converted to agriculture. The experiment was conducted in a completely randomized design with five replicates, in a 5x3 factorial arrangement: five areas and three aggregate classes (macro-, meso-, and microaggregates). The evaluated variables were: spore density and diversity of AMF, total glomalin, total organic carbon (TOC), total extraradical mycelium (TEM), and geometric mean diameter (GMD) of soil aggregates. A total of 21 AMF species was identified. Spore density varied from 29 to 606 spores per 50 mL of soil and was higher in microaggregates and in the area with 6 years of conversion to agriculture. Total glomalin was higher between murundus in all studied aggregate classes. The area with 6 years showed lower concentration of TOC in macroaggregates (8.6 g kg-1) and in microaggregates (10.1 g kg-1). TEM was greater at the top of the murundus in all aggregate classes. GMD increased with the conversion time to agriculture. The density and diversity of arbuscular mycorrhizal spores change with the conversion of fields of murundus into agriculture.

scholarly journals ARBUSCULAR MYCORRHIZAL FUNGI AND DARK SEPTATE ENDOPHYTIC FUNGI ON THE BIOMASS DEVELOPMENT OF VETIVER GRASS

2018 ◽  
Vol 31 (3) ◽  
pp. 602-611 ◽  
Author(s):  
JESSICA SILVA SANTOS ◽  
JACILENE FRANCISCA SOUZA SANTOS ◽  
LÁZARA JOSSIKARLA DE OLIVEIRA LOPES ◽  
JOHNY DE JESUS MENDONÇA ◽  
FRANCISCO SANDRO RODRIGUES HOLANDA ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Endophytic Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Northeastern Brazil ◽  
Vetiver Grass ◽  
Grass Family ◽  
Randomized Design ◽  
Fast Development ◽  
Completely Randomized Design

ABSTRACT Vetiver grass is a member of the grass family Poaceae. Its fast development is probably due to the interaction with native microbiota, whose influence has not been studied yet. The objective of this work was to evaluate the colonization and development of the vetiver grass (Chrysopogon zizanioides (L.) Roberty) inoculated with arbuscular mycorrhizal fungi and dark septate endophytic fungi. The experimental design was a completely randomized design with six treatments (control, without mycorrhizal fungi, native inoculants, UFLA05 - Gigaspora albida, UFLA351 - Rhizoglomus clarum, UFLA372 - Claroideoglomus etunicatum, and UFLA401 - Acaulospora morrowiae), with three replicates each. Vetiver grass tillers as well as the native microbial inoculum were obtained from the Lower São Francisco river experimental area, located in Sergipe state, Northeastern Brazil. There was a negative interaction between all tested UFLAs mycorrhizal isolates and the native microbiota (mycorrhizal and endophytic fungi) in the treatments, especially when taking into consideration plant height and volume of roots. The effects of inoculation with UFLA isolates may have been influenced by the presence of the native mycorrhizal fungi and the dark septate endophytic fungi. Vetiver grass was responsive to the native inoculant. The mycorrhizal colonization of the vetiver grass was vesicular, but the formation of the arbuscules can be influenced by the interaction between the fungus, plant, and the environment.

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