Preliminary Screening of Crude Extracts of Fagaropsis Angolensis for Anticancer Activity

2018 ◽  
Vol 8 (2) ◽  
pp. 75-80 ◽  
Author(s):  
Antony Letoyah Yiaile ◽  
James Mucunu Mbaria ◽  
Isaac Mpapuluu Ole-Mapenay ◽  
Mitchel Otieno Okumu ◽  
Abdi Hussein Hadun ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Preliminary Screening ◽  
Crude Extracts

scholarly journals Evaluating antibacterial and anticancer activity of crude extracts of bacterial endophytes from Crinum macowanii Baker bulbs

2019 ◽  
Vol 8 (12) ◽  
Author(s):  
Tendani E. Sebola ◽  
Nkemdinma C. Uche‐Okereafor ◽  
Kudzanai I. Tapfuma ◽  
Lukhanyo Mekuto ◽  
Ezekiel Green ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Bacterial Endophytes ◽  
Crude Extracts ◽  
Antibacterial And Anticancer Activity

scholarly journals Investigating the Anticancer Activity and Characterization of Bioactive Constituents of Moricandia sinaica (Boiss.) Boiss through In Vitro and In Silico Approaches in Triple-Negative Breast Cancer Cell Line

2021 ◽  
Vol 11 (3) ◽  
pp. 1244
Author(s):  
Muhammad Farooq Khan ◽  
Fahd A. Nasr ◽  
Almohannad A. Baabbad ◽  
Ali S. Alqahtani ◽  
Mohammad A. M. Wadaan
Keyword(s):  
Breast Cancer ◽  
Anticancer Activity ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Triple Negative Breast Cancer ◽  
In Silico ◽  
Triple Negative ◽  
Protein Target ◽  
Crude Extracts ◽  
M Phase

Resistance to chemotherapy and recurrence are major hurdles to treating hormone receptor-negative breast cancer. The crude extract and natural products obtained from medicinal plants are believed to be multitargeted and possess less toxicity as compared to synthetic compounds. The aerial parts and roots of Moricandia sinaica (Boiss.) Boiss were used to prepare the crude extracts in solvents of different polarities. Human breast cancer cell lines (MCF7 and MDA-MB-231), liver carcinoma (HepG2), and nontumorigenic cells of human origin (human umbilical vein endothelial cells (HUVEC)) were treated with a serial dilution of crude extracts obtained from the aerial and roots of Moricandia sinaica (Boiss.) Boiss. The methanol extract of the shoots exhibited a higher level of cytotoxicity against MDA-MB-231 cells than against any other cancer and nontumorigenic cells lines. Six new compounds were identified by gas chromatography–mass spectrophotometry analysis in the shoots extract of Moricandia sinaica (Boiss.) Boiss, and 2-Tridecen-1-ol was one of the major compounds that represent more than 35% of the extract. M-phase inducer phosphases 1 and 2 (CDC 25A and B) were identified as the specific protein target for 2-Tridecen-1-ol by the Swiss protein target prediction tool. In silico molecular docking showed the binding of 2-Tridecen-1-ol with CDC 25 B with a higher binding energy as compared to CDC 25A. The possible molecular mechanism of anticancer activity of Moricandia sinaica (Boiss.) Boiss in MDA-MB-231 breast cancer is through inhibition of M-phase inducer phosphatases 1 and 2 via 2-Tridecen-1-ol. Further investigations in breast cancer models are needed to explore the therapeutic potential of Moricandia sinaica (Boiss.) Boiss and 2-Tridecen-1-ol as an efficient remedy with a possibly less toxic approach to treat triple-negative breast cancer.

scholarly journals Crude and Chitosan Nano-particles Extracts of Some Maggots as Antioxidant and Anticancer Agents

2019 ◽  
pp. 1-8 ◽  
Author(s):  
Ahmed I. Hasaballah
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Crude Extract ◽  
Chitosan Nanoparticles ◽  
Radical Scavenging ◽  
Nano Particles ◽  
Liver Carcinoma ◽  
Crude Extracts ◽  
Hct 116

The antioxidant effects besides anticancer activities of Musca domestica, Lucilia sericata and Chrysomya albiceps maggots extracts against human liver carcinoma (HepG-2) and human colon carcinoma (HCT-116) were investigated. Two kinds of extracts, crude and chitosan nanoparticles (CNPs) were prepared. The antioxidant activity of different tested extracts was performed by DPPH radical scavenging method, the results obtained revealed that, the highest levels of DPPH scavenging activity were exhibited by the crude extracts of tested maggots with preference to C. albiceps extract, which exhibited a much more potent activity followed by L. sericata and M. domestica in crude and CNPs extracts. Crude extracts have lower anticancer activity than the CNPs extracts; however, the lowest percentage of cell viability (6.7±0.7%) was recorded by L. sericata crude extract against HCT-116, followed by C. albiceps crude extract (7.57±1.25%) against HepG-2 at the highest used concentration 100 µg/ml. The strongest anticancer activity was observed with CNPs extracts and it was recorded at concentrations of 80, 90 and 100 µg/ml against cell lines tested. Depending on Median inhibitory concentrations (IC50) of maggots crude and CNPs extracts, the IC50 values were in the range of 37.3 to 74.3 µg/ml and the highest anticancer activity was obtained by C. albiceps CNPs extracts against cell lines tested. In conclusion, both tested extracts have optimistic antioxidant activity. CNPs extracts have great therapeutic potential due to its anticancer inducing activities.

scholarly journals Anticancer Activity of Spirulina Cultivated in Walne and Organic Media

2019 ◽  
Vol 22 (1) ◽  
pp. 50 ◽  
Author(s):  
Putriana Sari Sirait ◽  
Iriani Setyaningsih ◽  
Kustiariyah Tarman
Keyword(s):  
Anticancer Activity ◽  
Crude Extract ◽  
Normal Breast ◽  
Selectivity Index ◽  
Organic Media ◽  
Active Components ◽  
Crude Extracts ◽  
Hoechst Dye ◽  
Apoptotic Activity ◽  
Mcf 7

Spirulina is Cyanobacteria containing active components which is potentially showing anticancer activity. The purposes of this study were to determine anticancer activity and selectivity of crude extracts<br />of Spirulina cultured using Walne and organic media, and to detect the apoptosis. The stages of this study included cultivation and harvesting of Spirulina, active components extraction, anticancer test and apoptosis<br />detection. Anticancer activity was determined using MTT assay. The crude extracts of Spirulina from Walne and organic cultures contained active components of alkaloids, flavonoids, steroids, and saponins. These<br />extracts were not toxic to normal breast cells (MCF-12a), but showed cytotoxic activity in breast cancer cells (MCF-7). The crude extract of Spirulina from Walne culture had IC50 value of 36.23 ppm and selectivity index 30.07, while the IC50 of organic culture was 117.78 ppm and selectivity index 7.17. Detection of apoptosis with Hoechst dye 33342 showed the apoptotic activity of Spirulina crude extract against MCF-7 cells.

scholarly journals Anticancer natural products from Aspergillus neoniger, an endophyte of Ficus carica

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Randa Abdou ◽  
Alaa M. Alqahtani ◽  
Gouda H. Attia
Keyword(s):  
Hela Cell ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Therapeutic Potential ◽  
Ficus Carica ◽  
Anticancer Activities ◽  
Preliminary Screening ◽  
Antiproliferative Effects ◽  
Hela Cell Lines ◽  
Screening Assays

Abstract Background Several plants have not been investigated for their endophytes, such as the medicinal plant Ficus carica for which anticancer activity has been confirmed. The endophyte Aspergillus neoniger was selected for investigation of its metabolites since it exerted anticancer activities in preliminary screening assays. Results Bioactivity-guided chromatographic fractionation was performed on the endophytic extract and resulted in the identification of asperazine and asperazine A through spectroscopic analysis. Moderate cytotoxicity against HeLa cell lines (CC50 = 18.4 µg mL−1) and moderate antiproliferative effects against HUVEC and K-562 cell lines (GI50 = 31.5 and 24.8 µg mL−1, respectively) were observed for asperazine. Asperazine A on the other hand showed weak cytotoxic activity against HeLa cell lines (CC50 = 34.6 µg mL−1) as well as weak cytostatic activities against HUVEC and K-562 cell lines (GI50 = 40.7 and 50.2 µg mL−1, respectively) while no antimicrobial activity was detected for both compounds. Conclusions These results suggest contribution of A. neoniger to the reported anticancer activity of the host plant and provides a new source of anticancer metabolites with therapeutic potential.

scholarly journals Marine bacteria from the Gulf of California with antimicrofouling activity against colonizing bacteria and microalgae

2018 ◽  
Vol 66 (4) ◽  
Author(s):  
Diana Elizabeth Sánchez-Rodríguez ◽  
Ismael Ortiz-Aguirre ◽  
Ruth Noemí Aguila-Ramírez ◽  
Erika Guadalupe Rico-Virgen ◽  
Bárbara González-Acosta ◽  
...  
Keyword(s):  
Gulf Of California ◽  
Antimicrobial Properties ◽  
Marine Microorganisms ◽  
Bacterial Strains ◽  
La Paz ◽  
Antifouling Paints ◽  
Preliminary Screening ◽  
Crude Extracts ◽  
Shewanella Algae ◽  
Inhibitory Potential

One way of reducing the input of pollutants into the marine environment is to enforce the use of non-toxic antifouling paints in marine protected areas. Thus, the purpose of this study was to detect marine microorganisms that secrete inhibitory substances against bacteria and microalgae to avoid biofouling on man-made structures in La Paz bay, B.C.S., Mexico. The inhibitory potential of 125 bacteria was evaluated against biofilm-forming bacteria. Crude extracts were obtained with methanol and ethyl acetate from 16 bacterial strains that exhibited antagonistic and antibacterial activity in a preliminary screening. Antibacterial and antimicroalgal assays were performed using crude extracts, the minimum inhibitory concentration (MIC) was determined. The highest activity against bacteria and microalgae was found in two strains, Shewanella algae and Staphylococcus sp. The results of this study suggest that extracts of bacteria from the Gulf of California with antimicrobial properties against biofilm-forming bacteria can also prevent the adhesion of microalgae, which may control the development of biofilm formation and, as a consequence, biofouling.

Sign in / Sign up

Export Citation Format

Share Document