ANTICANCER ACTIVITY OF THE CRUDE EXTRACTS AND AN ISOLATE (β-SITOSTEROL) FROM THE LEAF OF ANNONA MURICATA

2018 ◽  
Vol 7 (8) ◽  
Author(s):  
Nwaehujor Idorenyin Ugochi
Keyword(s):  
Anticancer Activity ◽  
Annona Muricata ◽  
Crude Extracts

In-Vitro Antioxidant, Antibacterial and Anticancer properties of Leaf Extract Annona Muricata

2021 ◽  
Vol 8 (1) ◽  
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Antioxidant Activities ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Annona Muricata ◽  
Anticancer Properties

Annona muricata is one of the important herbal plant that are widely used to treat antidiabetic, anti-inflammatory, insecticidal, antimalarial, anticancer, antibacterial and antioxidant activities. In the present study, extraction procedure was carried out in few step processes method. The extract contains high percentage of steroid, alkaloid, flavonoid, phenolic and saponin. The extract was used to study the antioxidant activity; antibacterial activities and anticancer activity by standard methods. The antioxidant activity was studied by using radical scavenging DPPH, FRAP and H2O2 method and disc diffusion method. The results of antibacterial activity of the following bacteria’s such as Pseudomonas, Staphylococcus, Bacillus, E.coli shows the maximum zone of inhibition against Staphylococcus aureus which is around 1.7 cm. The anticancer activity was carried out by MTT assay using Hep-G2 as cell line and results are reported in the paper.

1 H qNMR Quantification of Annonaceous Acetogenins in Crude Extracts of Annona muricata L. Fruit Pulp

2017 ◽  
Vol 28 (4) ◽  
pp. 251-256 ◽  
Author(s):  
Natacha Bonneau ◽  
Timothé Cynober ◽  
Jean-Christophe Jullian ◽  
Pierre Champy
Keyword(s):  
Annona Muricata ◽  
Fruit Pulp ◽  
Annonaceous Acetogenins ◽  
Crude Extracts

Preliminary Screening of Crude Extracts of Fagaropsis Angolensis for Anticancer Activity

2018 ◽  
Vol 8 (2) ◽  
pp. 75-80 ◽  
Author(s):  
Antony Letoyah Yiaile ◽  
James Mucunu Mbaria ◽  
Isaac Mpapuluu Ole-Mapenay ◽  
Mitchel Otieno Okumu ◽  
Abdi Hussein Hadun ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Preliminary Screening ◽  
Crude Extracts

scholarly journals Annona muricata: Comparative Assessment of the Antibacterial Activities of the Leaf and Stem Extracts against Multiple Antibiotic Resistant Clinical Isolates

2020 ◽  
pp. 12-21
Author(s):  
O. O. Agunloye ◽  
A. K. Onifade
Keyword(s):  
Antibacterial Effect ◽  
Cold Water ◽  
Water Extract ◽  
Antibacterial Activities ◽  
Hot Water ◽  
Annona Muricata ◽  
Antibiotic Resistant ◽  
Crude Extracts ◽  
Hot Water Extract ◽  
Zones Of Inhibition

This study was conducted to determine the antibacterial effect of crude extracts of Annona muricata, comparing the leaf and stem bark extracts using the same extraction solvents. The bacteria isolates were obtained from the hospital. The isolates obtained are Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis and Streptococcus pyogene; they were confirmed using standard techniques. Multiple antibiotic-resistant was confirmed in the isolates after antibiotic susceptibility testing. Extracts were prepared from the leaf and stem of A. muricata using ethanol, chloroform, cold water and hot water. The antibacterial activities of the crude extracts were assayed using the agar well diffusion method. The phytochemical screening revealed the presence of saponins, Tannins and flavonoid especially in the ethanol extracts of both leaf and stem part of the plant. All the extract showed varying degrees of antibacterial activities. Chloroform and Hot water rated best for antibacterial activities in this study. A. muricata stem hot water extract has zones of inhibition that ranges from 16.003 ± 0.009 mm to 1.000± 0.006 mm. A. muricata leaf hot water extract has antibacterial effect with zones ranging from 14.500± 0.009 mm to 1.000± 0.006 mm across the isolates. Cold water extracts recorded the lowest zones of inhibition from this study for the stem and Bark antibacterial activities. The stem of A. muricata has higher inhibitory effect on the test isolates compared to the leaf of      A. muricata.

scholarly journals Evaluating antibacterial and anticancer activity of crude extracts of bacterial endophytes from Crinum macowanii Baker bulbs

2019 ◽  
Vol 8 (12) ◽  
Author(s):  
Tendani E. Sebola ◽  
Nkemdinma C. Uche‐Okereafor ◽  
Kudzanai I. Tapfuma ◽  
Lukhanyo Mekuto ◽  
Ezekiel Green ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Bacterial Endophytes ◽  
Crude Extracts ◽  
Antibacterial And Anticancer Activity

scholarly journals Investigating the Anticancer Activity and Characterization of Bioactive Constituents of Moricandia sinaica (Boiss.) Boiss through In Vitro and In Silico Approaches in Triple-Negative Breast Cancer Cell Line

2021 ◽  
Vol 11 (3) ◽  
pp. 1244
Author(s):  
Muhammad Farooq Khan ◽  
Fahd A. Nasr ◽  
Almohannad A. Baabbad ◽  
Ali S. Alqahtani ◽  
Mohammad A. M. Wadaan
Keyword(s):  
Breast Cancer ◽  
Anticancer Activity ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Triple Negative Breast Cancer ◽  
In Silico ◽  
Triple Negative ◽  
Protein Target ◽  
Crude Extracts ◽  
M Phase

Resistance to chemotherapy and recurrence are major hurdles to treating hormone receptor-negative breast cancer. The crude extract and natural products obtained from medicinal plants are believed to be multitargeted and possess less toxicity as compared to synthetic compounds. The aerial parts and roots of Moricandia sinaica (Boiss.) Boiss were used to prepare the crude extracts in solvents of different polarities. Human breast cancer cell lines (MCF7 and MDA-MB-231), liver carcinoma (HepG2), and nontumorigenic cells of human origin (human umbilical vein endothelial cells (HUVEC)) were treated with a serial dilution of crude extracts obtained from the aerial and roots of Moricandia sinaica (Boiss.) Boiss. The methanol extract of the shoots exhibited a higher level of cytotoxicity against MDA-MB-231 cells than against any other cancer and nontumorigenic cells lines. Six new compounds were identified by gas chromatography–mass spectrophotometry analysis in the shoots extract of Moricandia sinaica (Boiss.) Boiss, and 2-Tridecen-1-ol was one of the major compounds that represent more than 35% of the extract. M-phase inducer phosphases 1 and 2 (CDC 25A and B) were identified as the specific protein target for 2-Tridecen-1-ol by the Swiss protein target prediction tool. In silico molecular docking showed the binding of 2-Tridecen-1-ol with CDC 25 B with a higher binding energy as compared to CDC 25A. The possible molecular mechanism of anticancer activity of Moricandia sinaica (Boiss.) Boiss in MDA-MB-231 breast cancer is through inhibition of M-phase inducer phosphatases 1 and 2 via 2-Tridecen-1-ol. Further investigations in breast cancer models are needed to explore the therapeutic potential of Moricandia sinaica (Boiss.) Boiss and 2-Tridecen-1-ol as an efficient remedy with a possibly less toxic approach to treat triple-negative breast cancer.

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