Effect of Epibrassinolide on Pigment Content, Total Protein Amount and Peroxidase Activity in Excised Cucumber Cotyledons

2013 ◽  
Vol 27 (1) ◽  
pp. 3502-3505
Author(s):  
Nihal Gören Sağlam
Keyword(s):  
Peroxidase Activity ◽  
Total Protein ◽  
Pigment Content ◽  
Cucumber Cotyledons

scholarly journals Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines

2002 ◽  
Vol 14 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Isabel Regina Prazeres de Souza ◽  
Vera Maria Carvalho Alves ◽  
Sidney Netto Parentoni ◽  
Antônio Carlos de Oliveira ◽  
Flávia França Teixeira ◽  
...  
Keyword(s):  
Protein Content ◽  
Inbred Line ◽  
Peroxidase Activity ◽  
Total Protein ◽  
Primary Root ◽  
Inbred Lines ◽  
Total Protein Content ◽  
Anionic Peroxidase ◽  
Sensitive Line ◽  
Peroxidase Isoforms

The effects of a short-term (80 min) exposure to 222 µM aluminum (Al) on the protein content and expression and on peroxidase activity and isoenzymes in the primary root of maize were evaluated. Two inbred lines differing in their level of tolerance to Al were used: Cateto 237 (tolerant) and L36 (sensitive). The apical 20 mm of the primary root was divided into 2-mm-long segments that were analyzed for total protein content and peroxidase activity. These results demonstrate that the total protein content along the root apex was not affected by Al in the tolerant inbred line, but decreased in the sensitive line. In the apical 2 mm of the root of the sensitive line, the expression of low molecular weight proteins (43 kDa or smaller) was decreased. Expression of low molecular proteins increased in the tolerant inbred line, even though total protein content did not increase. This suggests that some of these proteins could play a role in metal tolerance, perhaps as binding peptides. While the peroxidase activity of the tolerant inbred line did not change with exposure to Al, peroxidase activity in the apical 6 mm of the root of the sensitive line decreased. The tolerant inbred line constitutively expressed more anionic peroxidase isoforms. These results demonstrate that maintenance of protein expression may be an important component of the plant's resistance to Al stress, and that resistance to Al stress is associated with the higher expression of anionic peroxidase isoforms.

scholarly journals EFFECTS OF SOME HEAVY METALS ON TOTAL PROTEIN AND PEROXIDASE ACTIVITY IN CLOVER AND VETCH PLANTS

2020 ◽  
Vol 4 (4) ◽  
pp. 263-268
Author(s):  
Özlem DEMİRASLAN ◽  
Cüneyt AKI
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Peroxidase Activity ◽  
Total Protein ◽  
Lead Nitrate ◽  
Control Group ◽  
Distilled Water ◽  
Cadmium Nitrate ◽  
Maximum Increase ◽  
Forage Plants

Forage crop farming is shown as the most important way of continuous and safe forage production. Clover and vetch are the most used forage plants. In this research clover (Medicago sativa) and vetch (Vicia sativa) plants were planted in large pots (48x18x18cm) containing peat: perlite mixture (3:1). The seeds were kept in distilled water for 3 hours before planting and swelling was ensured. After the peat perlite mixture prepared in pots was watered sufficiently with distilled water and then seeds were planted. 1000 ppm heavy metal stock solutions have been prepared for lead nitrate Pb(NO3)2, copper nitrate Cu(NO3)2.3H2O, and cadmium nitrate (CdNO3)2.4H2O. Prepared heavy metal solutions were given to the plants at once, with irrigation water, in 300 mL for each pot. Total protein amount and peroxidase activity were examined by spectrophotometrically 72 hours after the heavy metal application. Three different heavy metals applications to the forage plants, have been showed different physiological reactions in these plants. These reactions have been measured by comparing control group of plants. According to research results, it was determined that the maximum increase in the total protein amount in M. sativa compared with the control group was 100 ppm applied lead. The maximum increase in the total protein amount in V. sativa compared with the control group was 30 ppm applied cadmium, respectively. The increase in peroxidase activity has been determined in M. sativa and V. sativa plants compared with the control groups were 30 ppm applied cadmium. As a result of our research, it was determined that cadmium has been found to be more effective than lead and copper for stimulation of plant defense system.

scholarly journals Erratum to: The effects of two preservatives on peroxidase activity and total protein content in Strelitzia reginae during cold storage

2016 ◽  
Vol 57 (3) ◽  
pp. 309-309
Author(s):  
Marcos R. da S. Vieira ◽  
Luiz G. M. Pessoa ◽  
Walter S. E. Júnior ◽  
Ygor H. Leal ◽  
Luzia F. da Silva ◽  
...  
Keyword(s):  
Protein Content ◽  
Cold Storage ◽  
Peroxidase Activity ◽  
Total Protein ◽  
Total Protein Content ◽  
Strelitzia Reginae

Effects of caffeine treatment alone and in combination with gamma exposure on cultured peanut cells

1984 ◽  
Vol 62 (9) ◽  
pp. 1890-1895 ◽  
Author(s):  
M. Inoue ◽  
R. B. van Huystee
Keyword(s):  
Ionic Strength ◽  
Cell Growth ◽  
Mitotic Index ◽  
Peroxidase Activity ◽  
Total Protein ◽  
High Ionic Strength ◽  
Suspension Cells ◽  
Caffeine Treatment ◽  
Qualitative Changes

Cell growth, mitotic index, and peroxidase activity were measured, and radioimmunoassay with [35S]methionine for peroxidase synthesis was carried out on peanut suspension cells treated with 10−5 M caffeine with or without 50 kR gamma exposure. Decreases in the amount of peroxidase in the medium from caffeine treatment were thought to be induced by events causing simultaneous mitotic reduction and degradation of peroxidase by gamma exposure. However, the data based on a comparison of the rate of total protein and of peroxidase synthesis suggest that caffeine treatment induces quantitative and qualitative changes in the rate of peroxidase synthesis. The data on qualitative differences were obtained from peroxidase extraction without or with high ionic strength buffer.

scholarly journals Continuous UV-B Irradiation Induces Endoreduplication and Peroxidase Activity in Epidermal Cells Surrounding Trichomes on Cucumber Cotyledons

2010 ◽  
Vol 51 (2) ◽  
pp. 187-196 ◽  
Author(s):  
Seiji YAMASAKI ◽  
Eri SHIMADA ◽  
Tomoe KUWANO ◽  
Tae KAWANO ◽  
Naoto NOGUCHI
Keyword(s):  
Peroxidase Activity ◽  
Epidermal Cells ◽  
Cucumber Cotyledons
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