scholarly journals EFFECTS OF SOME OF HEAVY METALS ON TOTAL PROTEIN AMOUNT AND PEROXIDASE ACTIVITY IN SOLANUM LYCOPERSICUM MILL

2019 ◽  
Vol 3 (4) ◽  
pp. 371-378
Author(s):  
Gülru YÜCEL ◽  
Cüneyt AKI
Keyword(s):  
Heavy Metals ◽  
Solanum Lycopersicum ◽  
Peroxidase Activity ◽  
Total Protein

scholarly journals EFFECTS OF SOME HEAVY METALS ON TOTAL PROTEIN AND PEROXIDASE ACTIVITY IN CLOVER AND VETCH PLANTS

2020 ◽  
Vol 4 (4) ◽  
pp. 263-268
Author(s):  
Özlem DEMİRASLAN ◽  
Cüneyt AKI
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Peroxidase Activity ◽  
Total Protein ◽  
Lead Nitrate ◽  
Control Group ◽  
Distilled Water ◽  
Cadmium Nitrate ◽  
Maximum Increase ◽  
Forage Plants

Forage crop farming is shown as the most important way of continuous and safe forage production. Clover and vetch are the most used forage plants. In this research clover (Medicago sativa) and vetch (Vicia sativa) plants were planted in large pots (48x18x18cm) containing peat: perlite mixture (3:1). The seeds were kept in distilled water for 3 hours before planting and swelling was ensured. After the peat perlite mixture prepared in pots was watered sufficiently with distilled water and then seeds were planted. 1000 ppm heavy metal stock solutions have been prepared for lead nitrate Pb(NO3)2, copper nitrate Cu(NO3)2.3H2O, and cadmium nitrate (CdNO3)2.4H2O. Prepared heavy metal solutions were given to the plants at once, with irrigation water, in 300 mL for each pot. Total protein amount and peroxidase activity were examined by spectrophotometrically 72 hours after the heavy metal application. Three different heavy metals applications to the forage plants, have been showed different physiological reactions in these plants. These reactions have been measured by comparing control group of plants. According to research results, it was determined that the maximum increase in the total protein amount in M. sativa compared with the control group was 100 ppm applied lead. The maximum increase in the total protein amount in V. sativa compared with the control group was 30 ppm applied cadmium, respectively. The increase in peroxidase activity has been determined in M. sativa and V. sativa plants compared with the control groups were 30 ppm applied cadmium. As a result of our research, it was determined that cadmium has been found to be more effective than lead and copper for stimulation of plant defense system.

Alteration in growth and peroxidase activity by heavy metals in Phaseolus seedlings

2002 ◽  
Vol 24 (1) ◽  
pp. 89-95 ◽  
Author(s):  
N. G. Parmar ◽  
S. D. Vithalani ◽  
S. V. Chanda
Keyword(s):  
Heavy Metals ◽  
Peroxidase Activity

scholarly journals Heavy metal concentration and health risk assessment in commonly sold vegetables in Dhaka city market

2019 ◽  
Vol 54 (4) ◽  
pp. 357-366
Author(s):  
M Sultana ◽  
MN Mondol ◽  
AA Mahir ◽  
R Sultana ◽  
SF Elahi ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Brassica Oleracea ◽  
Solanum Lycopersicum ◽  
Cucumis Sativus ◽  
Health Risks ◽  
Daily Intake ◽  
Amaranthus Cruentus ◽  
Ipomoea Aquatica ◽  
Basella Alba

This study assesses the levels of heavy metals in vegetables (Cucumis sativus, Solanum lycopersicum, Ipomoea aquatica, Amaranthus cruentus, Basella alba, Brassica oleracea, Musa acuminata) collected from Kawran Bazar vegetable market located in Dhaka, Bangladesh. Also, it examines potential health risks from the consumption of these vegetables. The samples were randomly collected, processed and analyzed for heavy metals using Atomic Absorption Spectrophotometer. Among the vegetables Basella alba had the highest heavy metal content followed by Cucumis sativus, Amaranthus cruentus, Solanum lycopersicum, Brassica oleracea, Musa acuminate and Ipomoea aquatica. The average daily intake for Cr (0.245 mg/person/day) was above the permissible maximum tolerable daily intake of 0.20 mg/person/day endorsed by WHO/FAO (2013). The hazard quotient (HQ) values for Cd in Basella alba (4.400) and Brassica oleracea (1.333), for Cr in Ipomoea aquatica (1.756), Amaranthus cruentus (1.655), Basella alba (3.033) and Musa acuminate (1.333) as well as the hazard indices (HI) for I. aquatica (2.537), A. cruentus (2.791), B.alba (8.883), B. oleracea (2.295), M. acuminate (2.999) exceeded unity, signifying presence of health risks from consumption of the vegetables. This study recommends regular monitoring of heavy metals in vegetables and foodstuffs to prevent excessive accrual in food chain. Bangladesh J. Sci. Ind. Res.54(4), 357-366, 2019

scholarly journals Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines

2002 ◽  
Vol 14 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Isabel Regina Prazeres de Souza ◽  
Vera Maria Carvalho Alves ◽  
Sidney Netto Parentoni ◽  
Antônio Carlos de Oliveira ◽  
Flávia França Teixeira ◽  
...  
Keyword(s):  
Protein Content ◽  
Inbred Line ◽  
Peroxidase Activity ◽  
Total Protein ◽  
Primary Root ◽  
Inbred Lines ◽  
Total Protein Content ◽  
Anionic Peroxidase ◽  
Sensitive Line ◽  
Peroxidase Isoforms

The effects of a short-term (80 min) exposure to 222 µM aluminum (Al) on the protein content and expression and on peroxidase activity and isoenzymes in the primary root of maize were evaluated. Two inbred lines differing in their level of tolerance to Al were used: Cateto 237 (tolerant) and L36 (sensitive). The apical 20 mm of the primary root was divided into 2-mm-long segments that were analyzed for total protein content and peroxidase activity. These results demonstrate that the total protein content along the root apex was not affected by Al in the tolerant inbred line, but decreased in the sensitive line. In the apical 2 mm of the root of the sensitive line, the expression of low molecular weight proteins (43 kDa or smaller) was decreased. Expression of low molecular proteins increased in the tolerant inbred line, even though total protein content did not increase. This suggests that some of these proteins could play a role in metal tolerance, perhaps as binding peptides. While the peroxidase activity of the tolerant inbred line did not change with exposure to Al, peroxidase activity in the apical 6 mm of the root of the sensitive line decreased. The tolerant inbred line constitutively expressed more anionic peroxidase isoforms. These results demonstrate that maintenance of protein expression may be an important component of the plant's resistance to Al stress, and that resistance to Al stress is associated with the higher expression of anionic peroxidase isoforms.

scholarly journals Seasonal effects of heavy metals on the date mussel Lithophaga lithophaga (Mollusca:Bivalvia) at Eastern harbor, Alexandria, Egypt

2020 ◽  
Vol 1 (1) ◽  
pp. 62-77
Author(s):  
Shahenaz M. Abd-Ellah ◽  
◽  
Soheir El-Sherif ◽  
Rehab El-Morshedy ◽  
◽  
...  
Keyword(s):  
Heavy Metals ◽  
Protein Content ◽  
Digestive Gland ◽  
Total Protein ◽  
Soft Tissues ◽  
Food Sources ◽  
Total Protein Content ◽  
Ultrastructural Studies ◽  
Different Tissues ◽  
Lithophaga Lithophaga

The edible mussel Lithophaga lithophaga is considered as one of the most important human food sources in Alexandria, Mediterranean Sea. The present study is designed to determine the seasonal bioaccumulation levels of Cd, Co and Pb in the whole soft tissues as well as different tissues of Lithophaga lithophaga. Results revealed that the seasonal bioaccumulation levels of Cd, Co and Pb in date mussel were below the permissible limits or other reported values from other regions of the Mediterranean. On the other hand, the order of metals accumulation level in different tissues was as follows: digestive gland>remaining soft tissues>gonads. The present study confirmed the role of digestive gland as a concentration center for heavy metals. Moreover, total protein content and stress protein responses of the whole soft tissues were evaluated. The total protein content was arranged in the following order: summer>autumn>spring>winter. Five novel stress proteins appeared in summer. The histological and ultrastructural studies of the digestive gland of Lithophaga lithophaga collected in summer and spring showed marked histopathological alternations.

Effect of Antioxidants on Heavy Metals Induced Conformational Altera-tion of Cytochrome C And Myoglobin

2020 ◽  
Vol 27 ◽  
Author(s):  
Anwar Ahmed ◽  
Khadega Khamis Moh Alazoumi ◽  
Salman Freeh Alamery ◽  
Anas Shamsi ◽  
Basir Ahmad ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Cytochrome C ◽  
Peroxidase Activity ◽  
Heme Proteins ◽  
Deleterious Effect ◽  
Tertiary Structure ◽  
Animal Origin ◽  
Metal Exposure ◽  
Secondary And Tertiary Structure

Background: The exposure to heavy metals due to unrestrained industrialization, pollution and non-degradability imposes a significant risk to human health. Proteins are prime targets of heavy metal stress, however, the underlying mechanisms and its impact on heme proteins is still not entirely clear. Objective: To analyze the deleterious effect of heavy metals such as cadmium, chromium and mercury on conformation of two proteins namely, cytochrome c and myoglobin. The protective effect of glycine and ascorbic acid (animal origin), gallic acid and sesamol (plant origin) on heavy metal exposure was studied. Methods: Far- and near-UV circular dichroism (CD) measurements monitored the changes in secondary and tertiary structure. Absorption Soret spectroscopy study revealed changes in heme-protein interaction. Peroxidase activity has been assayed to measure the absorption of tetraguaiacol. The interaction of heme proteins with different heavy metals was done using docking study. Result: Far- and near–UV CD measurements reveal that heavy metals disrupt the secondary and tertiary structure of heme proteins. Antioxidants counteract the deleterious effect of heavy metals. Absorption spectroscopy revealed changes in the Soret region of these heme proteins. Changes in peroxidase activity was observed on addition of heavy metals and antioxidants. Molecular docking validated interaction of the heavy metals with proteins with a significant binding affinity (-2.3 kcal/mol). Conclusion: Heavy metals interfered and disrupted both the heme proteins and mercury showed the maximum deleterious effect, further, chromium showed detrimental effect at very small concentration. The antioxidants from animal origin exhibited better protective response than those from plant source.

Peroxidase Activity after Viral Infection and Whitefly Infestation in Juvenile and Mature Leaves of Solanum lycopersicum

2011 ◽  
Vol 159 (11-12) ◽  
pp. 707-712 ◽  
Author(s):  
Hamady Dieng ◽  
Tomomitsu Satho ◽  
Ahmad Abu Hassan ◽  
Al Thbiani Aziz ◽  
Ronald Enrique Morales ◽  
...  
Keyword(s):  
Viral Infection ◽  
Solanum Lycopersicum ◽  
Peroxidase Activity ◽  
Mature Leaves

Biochar effect on crop performance and Pb and Zn uptake of tomato (Solanum lycopersicum, L.) plants grown on heavy metals contaminated Kosovo soils

2020 ◽  
Vol 55 (9) ◽  
pp. 844-853
Author(s):  
Hana Voca ◽  
Lea Piscitelli ◽  
Giuseppe Natale Mezzapesa ◽  
Donato Mondelli ◽  
Teodoro Miano ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L ◽  
Zn Uptake ◽  
Crop Performance
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