scholarly journals Response of Fumaric Acid Addition on Methanogenesis, Rumen Fermentation, and Dry Matter Degradability in Diets Containing Wheat Straw and Sorghum or Berseem as Roughage Source

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
S. K. Sirohi ◽  
Poonam Pandey ◽  
Navneet Goel

An in vitro incubation system was used to evaluate effect of supplementation of fumaric acid at 0, 5, 10, and 15 mM concentration in high-, medium-, and low-fiber wheat straw containing total mixed diets with sorghum (Sorghum vulgare) and berseem clover (Trifolium alexandrinum L.) on rumen fermentation, methane production, and gas kinetics parameters. Three types of diets were prepared with different roughage and concentrate ratio (80 : 20, 50 : 50, and 20 : 80) by taking the representative samples. The roughage part composed of wheat straw (70 parts) and sorghum (30 parts) or berseem (30 parts) and the concentrate part composed of maize (33%), GNC (21%), mustard cake (12%), wheat bran (20%), deoiled rice bran (11%), mineral mixture (2%), and salt (1%). Fumaric acid was added in incubation medium to achieve final concentration of 0, 5, 10, and 15 mM. All the treatment combinations were arranged in 4×3 factorial designs with three replicates. It was concluded from the study that fumaric acid addition (5–15 mM) in diets varying in roughage to concentrate ratio significantly (P≤0.05) reduced the methane production without affecting dry matter digestibility and maximum reduction was noticed at 5 mM concentration.

2017 ◽  
Vol 48 (2) ◽  
pp. 63-69
Author(s):  
M. Joch ◽  
V. Kudrna ◽  
B. Hučko

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.


Author(s):  
A. S. Chaudhry ◽  
E. L. Miller

That alkali treatments can improve the nutritional quality of poor quality roughages has long been established (Sundstol and Owen, 1984). However, their effectiveness is limited by their potential hazards to the animals and mankind. Alkaline hydrogen peroxide (AHP) has recently emerged as a possible substitute (Gould, 1985) but its farm scale application is limited by the need for high amounts of chemicals and water. Lack of any information regarding its effectiveness over NaOH alone is another factor which requires further investigation. The present study was, therefore, planned to assess the effectiveness of pH-regulated (11.5±0.2) H2O2 (AHP) in improving the in vitro dry matter digestibility (IVDMD) of wheat straw (WS, Avalon) under different laboratory conditions. The possibility of using CaO on its own or to regulate pH for AHP was also tested.


2021 ◽  
Author(s):  
Vithal K. Munde ◽  
Asit Das ◽  
Putan Singh ◽  
Ashok K. Verma ◽  
Nirmala Muwel ◽  
...  

Abstract This experiment was conducted to study the effect of supplementation of Kappaphycus alvarezii (KA) and Gracilaria salicornia (GS) in vitro fermentation pattern, total gas and methane production of mixed substrates. Basal substrate comprising of concentrates and wheat straw (50:50) was supplemented with either 0% (control), 1 (KA1), 2 (KA2), 4 (KA4), 6 (KA6), and 8 % (KA8) of Kappaphycus; and, 1 (GS1), 2 (GS2), 4 (GS4), 6 (GS6), and 8 (GS8 ) of Gracilaria, respectively. Asymptote, rate constant of gas production and t-half, concentration of total volatile fatty acids (TVFA), and in vitro dry matter digestibility (IVDMD) was not affected up to 2% level KA supplementation, beyond which asymptote, and rate constant of gas production, TVFA, and IVDMD decreased and t-half increased (P<0.001). Asymptote, rate constant of gas production, TVFA and IVDMD was not affected at 1% level of inclusion, beyond which a steady decline in these parameters was observed (P<0.001). Methane production (ml/g DM) was higher (P<0.001) in CON, followed by KA1 and KA2, and lower values were observed in by KA4, KA6 and KA8. Methane production (ml/kg DM) declined (P<0.001) steadily with increased level of GS in the substrates. From the results it was concluded that inclusion of Kappaphycus alvarezii and Gracilaria salicornia at 2 and 1%, respectively in the fermentation substrate can reduce in vitro methane production without any adverse impact on total gas production and in vitro dry matter digestibility.


1960 ◽  
Vol 54 (3) ◽  
pp. 417-420 ◽  
Author(s):  
G. D. Phillips ◽  
R. E. Hungate ◽  
A. MacGregor ◽  
D. P. Hungate

1. Experiments are described in which retention time of digesta in the reticulo-rumen, fermentation rates of rumen contents, and dry-matter digestibilities were studied simultaneously in four grade European and three zebu steers.2. Fermentation rates and rumen retentions were significantly negatively correlated.3. Correlations between digestibility and the other two factors were not significant at a high level.4. The multiple regressions calculated for retention time and fermentation rate were significant at the 5% level and that for digestibility approached this level.5. While only fermentation rates show significant differences for the two types of cattle, the results suggest that grades and zebus differ also in the rate of passage of digesta through the rumen.6. The loss in weight of substrate per unit of fermentation products was measured inin vitroexperiments.7. Using certain assumptions, estimates are made of the extent to which the measured fermentation rates could account for the loss in weight of dry matter during digestion, and are compared with the loss actually found.


Author(s):  
A. S. Chaudhry ◽  
E. L. Miller

Previous studies (Chaudhry and Miller,1990) have shown the effectiveness of CaO, NaOH and alkaline hydrogen peroxide (AHP) in improving the in vitro dry matter digestibility (IVDMD) of wheat straw (WS). Based on those results, therefore, an experiment was planned to test the effectiveness of some of the selected treatments in improving ruminal and total tract digestibilities by sheep of WS (Norman), treated on a large scale. Rumen conditions were also characterised to see if there was any relationship between digestion and the rumen conditions.


2016 ◽  
Vol 56 (10) ◽  
pp. 1700
Author(s):  
J. M. Cantet ◽  
D. Colombatto ◽  
G. Jaurena

The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.


1997 ◽  
Vol 37 (6) ◽  
pp. 617 ◽  
Author(s):  
A. S. Chaudhry

Summary. This study compared the effect of different levels (24–160 g/kg DM) of NaOH alone or in combination with 2 levels (130 or 260 g/kg straw DM) of H2O2 (alkaline hydrogen peroxide, AHP) in improving in vitro dry matter digestibility of wheat straw. The study also examined the role of filtration or a water wash of straws treated at a regulated pH (± s.d.) of 11.5 ± 0.2 to maximise the effects of NaOH and AHP on in vitro dry matter digestibility. The experiment was a 3 × 2 × 3 factorial design, replicated twice, and included 3 straw groups (pH-control, NaOH-control, AHP), each treated with 2 volumes of chemical solutions (high, 26 L/kg DM containing 48 or 160 g NaOH alone or 160 g NaOH plus 260 g H2O2 ; low, 13 L/kg DM containing 24 or 80 g NaOH alone or 80 g NaOH plus 130 g H2O2 ) and each was subdivided into 3 groups for storage (unfiltered, filtered, washed). Both NaOH-control and AHP reduced (P<0.001) neutral detergent fibre content and increased (P<0.001) in vitro dry matter digestibility of straw compared with pH-control. However, AHP was more effective (P<0.001) in reducing neutral detergent fibre and increasing in vitro dry matter digestibility of straw compared with NaOH alone. Filtration and washing of treated straws increased (P<0.001) neutral detergent fibre and reduced (P<0.001) in vitro dry matter digestibility compared with unfiltered samples of treated straws. High volumes of chemicals were more effective (P<0.001) in reducing neutral detergent fibre content and improving in vitro dry matter digestibility of straws compared with those treated with low volumes of chemicals. While regulation of pH around 11.5 was effective in reducing neutral detergent fibre and enhancing in vitro dry matter digestibility the use of filtration or a water wash of straws following chemical treatments is not recommended.


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