Methane production and in vitro digestibility of low quality forages treated with a protease or a cellulase

2016 ◽  
Vol 56 (10) ◽  
pp. 1700
Author(s):  
J. M. Cantet ◽  
D. Colombatto ◽  
G. Jaurena
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Neutral Detergent Fibre ◽  
Dry Matter Digestibility ◽  
Application Rates ◽  
The Impact

The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.

scholarly journals Influence of Kappaphycus Alvarezii and Gracilaria Salicornia Supplementation on in Vitro Fermentation Pattern, Total Gas and Methane Production of Mixed Substrates

2021 ◽  
Author(s):  
Vithal K. Munde ◽  
Asit Das ◽  
Putan Singh ◽  
Ashok K. Verma ◽  
Nirmala Muwel ◽  
...  
Keyword(s):  
Rate Constant ◽  
Methane Production ◽  
Dry Matter ◽  
Kappaphycus Alvarezii ◽  
Gas Production ◽  
Mixed Substrates ◽  
Gracilaria Salicornia ◽  
Dry Matter Digestibility ◽  
Fermentation Pattern

Abstract This experiment was conducted to study the effect of supplementation of Kappaphycus alvarezii (KA) and Gracilaria salicornia (GS) in vitro fermentation pattern, total gas and methane production of mixed substrates. Basal substrate comprising of concentrates and wheat straw (50:50) was supplemented with either 0% (control), 1 (KA1), 2 (KA2), 4 (KA4), 6 (KA6), and 8 % (KA8) of Kappaphycus; and, 1 (GS1), 2 (GS2), 4 (GS4), 6 (GS6), and 8 (GS8 ) of Gracilaria, respectively. Asymptote, rate constant of gas production and t-half, concentration of total volatile fatty acids (TVFA), and in vitro dry matter digestibility (IVDMD) was not affected up to 2% level KA supplementation, beyond which asymptote, and rate constant of gas production, TVFA, and IVDMD decreased and t-half increased (P<0.001). Asymptote, rate constant of gas production, TVFA and IVDMD was not affected at 1% level of inclusion, beyond which a steady decline in these parameters was observed (P<0.001). Methane production (ml/g DM) was higher (P<0.001) in CON, followed by KA1 and KA2, and lower values were observed in by KA4, KA6 and KA8. Methane production (ml/kg DM) declined (P<0.001) steadily with increased level of GS in the substrates. From the results it was concluded that inclusion of Kappaphycus alvarezii and Gracilaria salicornia at 2 and 1%, respectively in the fermentation substrate can reduce in vitro methane production without any adverse impact on total gas production and in vitro dry matter digestibility.

scholarly journals In Vitro Ruminal Fermentation and Degradability of Rice Husk on Rice Bran Substitution

2020 ◽  
Vol 24 (1) ◽  
pp. 50
Author(s):  
Rusli Fidriyanto ◽  
Roni Ridwan ◽  
Wulansih Dwi Astuti ◽  
Rohmatussolihat Rohmatussolihat ◽  
Nurul Fitri Sari ◽  
...  
Keyword(s):  
Organic Matter ◽  
Rice Husk ◽  
Rice Bran ◽  
Dry Matter ◽  
Acid Level ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Fatty Acid Production ◽  
Dry Matter Digestibility

Rice bran is a by-product of the rice milling process and has been well used as livestock feed. Rice bran is often adulterated with rice husk. The objective of this study was to evaluate the in vitro ruminal fermentation characteristics of rice bran with various compositions of rice husk and assess the relationship between rice husk addition and rice bran quality. The experiment was arranged in a completely randomized design with rice husk addition as a factor and three replications. Data of proximate value, gas production, ruminal degradability, and volatile fatty acid production were analyzed by analysis of variance. Moreover, significant effects of each treatment in the in vitro fermentation were further analyzed by Duncan's multiple range test (P<0.05). It was shown that the addition of rice husk to rice bran could increase acetic acid level, but it reduced potential gas production, gas production rate, organic matter and dry matter digestibility, and propionic acid level. Interestingly, the linear regression of dry matter digestibility, organic matter digestibility, and potential gas production showed the high adjusted R2 values. Moreover, this study also revealed that 10% of rice husk substitution on rice bran could significantly reduce the dry matter digestibility.

scholarly journals Flavonoids and their aromatic derivatives in Piper betle powder promote in vitro methane mitigation in a variety of diets

2020 ◽  
Vol 44 ◽  
Author(s):  
Rayudika Aprilia Patindra Purba ◽  
Siwaporn Paengkoum ◽  
Chalermpon Yuangklang ◽  
Pramote Paengkoum
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Methane Emissions ◽  
Gas Production ◽  
Piper Betle ◽  
Ruminal Fermentation ◽  
Perennial Plant ◽  
Rumen Ph ◽  
Lactating Goats

ABSTRACT At present, there is little information regarding whether supplementation with Piper betle powder (PBP) and sunflower oil (SFO) has a synergistic effect on lowering methane emissions without negatively impacting ruminal fermentation. This study investigated the effects of PBP, supplemented either with or without SFO, on biogas release, fermentation end-products, and microorganisms in the rumen of lactating goats. The treatments were run in a completely randomized 3 × 5 factorial arrangement, whereby 0, 15, and 30 mg SFO were combined with 0, 15, 30, 45, and 60 mg PBP on a dry matter basis. The outcomes were assessed in vitro. PBP was obtained from the perennial plant Piper betle L., which is an abundant source of flavonoids and their aromatic derivatives. SFO, which reduces dietary methane emissions, was supplemented to confirm whether it interacted with other nutrients in the ruminant diet. SFO × PBP significantly (p < 0.05) decreased methane production, enhanced total volatile fatty acid concentrations, and decreased the number of rumen protozoa. We found that 15-30 mg, but not 45-60 mg, PBP combined with 0, 15, and 30 mg SFO increased (p < 0.05) total gas production (including CO2) from fermentation. However, our results suggested that at least 45 mg PBP, either alone or combined with SFO, was required to reduce ammonia-N (p < 0.05). Not all treatments affected rumen pH. In conclusion, supplementing PBP (< 30 mg), either alone or combined with SFO, has a suppressing effect on methane production while preserving an optimum rate of rumen fermentation.

Washing and filtration of wheat straw treated with sodium hydroxide alone or with hydrogen peroxide to modify cell wall composition and in vitro digestibility

1997 ◽  
Vol 37 (6) ◽  
pp. 617 ◽  
Author(s):  
A. S. Chaudhry
Keyword(s):  
Hydrogen Peroxide ◽  
Wheat Straw ◽  
Dry Matter ◽  
Ph Control ◽  
Fibre Content ◽  
In Vitro Digestibility ◽  
Neutral Detergent Fibre ◽  
Chemical Treatments ◽  
Dry Matter Digestibility

Summary. This study compared the effect of different levels (24–160 g/kg DM) of NaOH alone or in combination with 2 levels (130 or 260 g/kg straw DM) of H2O2 (alkaline hydrogen peroxide, AHP) in improving in vitro dry matter digestibility of wheat straw. The study also examined the role of filtration or a water wash of straws treated at a regulated pH (± s.d.) of 11.5 ± 0.2 to maximise the effects of NaOH and AHP on in vitro dry matter digestibility. The experiment was a 3 × 2 × 3 factorial design, replicated twice, and included 3 straw groups (pH-control, NaOH-control, AHP), each treated with 2 volumes of chemical solutions (high, 26 L/kg DM containing 48 or 160 g NaOH alone or 160 g NaOH plus 260 g H2O2 ; low, 13 L/kg DM containing 24 or 80 g NaOH alone or 80 g NaOH plus 130 g H2O2 ) and each was subdivided into 3 groups for storage (unfiltered, filtered, washed). Both NaOH-control and AHP reduced (P<0.001) neutral detergent fibre content and increased (P<0.001) in vitro dry matter digestibility of straw compared with pH-control. However, AHP was more effective (P<0.001) in reducing neutral detergent fibre and increasing in vitro dry matter digestibility of straw compared with NaOH alone. Filtration and washing of treated straws increased (P<0.001) neutral detergent fibre and reduced (P<0.001) in vitro dry matter digestibility compared with unfiltered samples of treated straws. High volumes of chemicals were more effective (P<0.001) in reducing neutral detergent fibre content and improving in vitro dry matter digestibility of straws compared with those treated with low volumes of chemicals. While regulation of pH around 11.5 was effective in reducing neutral detergent fibre and enhancing in vitro dry matter digestibility the use of filtration or a water wash of straws following chemical treatments is not recommended.

scholarly journals PSII-5 Chemical composition, in vitro dry matter digestibility, gas production and methane emission of maintenance, growth and production diets/rations of buffaloes

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 408-408
Author(s):  
Uchenna Anele ◽  
Sultan Singh ◽  
B P Kushwaha ◽  
P K Gupta ◽  
S Bhattacharya
Keyword(s):  
Chemical Composition ◽  
Methane Production ◽  
Dry Matter ◽  
Chemical Constituents ◽  
Gas Production ◽  
Protein Fractions ◽  
Mean Values ◽  
Dry Matter Digestibility ◽  
Structural Carbohydrate

Abstract Ten types of diets were prepared to meet the nutrient requirements of buffaloes for maintenance (MD1 to MD10), growth (GD1 to GD10) and production/lactation (PD1 to PD10). A total of 30 diets were evaluated for chemical composition, in vitro gas and methane production, and dry matter (DM) disappearance using buffalo rumen liquor. The CNCPS was used to estimate the carbohydrate and protein fractions. Protein fractions PB1 and PB2 of maintenance diets were lower (P &lt; 0.05) than growth and production diets, while protein fractions (PB3) and Pc were (P &lt; 0.05) higher in maintenance than in growth and production diets. Mean values of PA was (P &lt; 0.05) higher in growth diets (136.9) than production (114.8) and maintenance diets (105.6 g/kg DM). Maintenance diets had (P &lt; 0.05) higher structural carbohydrate contents (586.2 g/kg DM) than production diets (513.0 g/kg DM). Carbohydrate fraction (CB1) was highest (P &lt; 0.05) in production diets (187.2 g/kg DM) followed by growth (129.5 g/kg DM) and maintenance diets (96.1 g/kg DM). In vitro gas production at different time periods (12, 24 and 48 h) was similar for maintenance (63.04, 51.98 and 48.15 ml/g DM), growth (63.83, 52.73 and 48.250) and production diets (63.51, 52.54 and 47.21 ml/g DM). Cumulative methane production was numerically lower for maintenance (28.40 ml/g DM) than growth (29.58 ml/g DM) and production diets (33.13 ml/g DM). In vitro methane production as a proportion of degraded DM (ml/g DDM and g/kg DDM) was similar for maintenance (14.21 and 29.53), growth (42.19 and 30.25) and production diets (41.26 and 29.58). Dietary chemical constituents such as EE, lignin, NDIN, ADIN and PB3 and Cc were (P &lt; 0.05) negatively associated with methane production, while OM, NPN, SP, PA and PB1, TCHO and CB2 were positively (P &lt; 0.05) correlated with methane production.

In vitro ruminal fermentation characteristics and methane production differ in selected key pasture species in Australia

2013 ◽  
Vol 64 (9) ◽  
pp. 935 ◽  
Author(s):  
B. K. Banik ◽  
Z. Durmic ◽  
W. Erskine ◽  
K. Ghamkhar ◽  
C. Revell
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
Enteric Fermentation ◽  
Rumen Microbes ◽  
Nutritive Values ◽  
The Impact ◽  
In Vitro Rumen Fermentation

Thirteen current and potential pasture species in southern Australia were examined for differences in their nutritive values and in vitro rumen fermentation profiles, including methane production by rumen microbes, to assist in selection of pasture species for mitigation of methane emission from ruminant livestock. Plants were grown in a glasshouse and harvested at 7 and 11 weeks after sowing for in vitro batch fermentation, with nutritive values assessed at 11 weeks of growth. The pasture species tested differed significantly (P < 0.001) in methane production during in vitro rumen fermentation, with the lowest methane-producing species, Biserrula pelecinus L., producing 90% less methane (4 mL CH4 g–1 dry matter incubated) than the highest methane-producing species, Trifolium spumosum L. (51 mL CH4 g–1 dry matter incubated). Proxy nutritive values of species were found not to be useful predictors of plant fermentation characteristics or methane production. In conclusion, there were significant differences in fermentative traits, including methane production, among selected pasture species in Australia, indicating that the choice of fodder species may offer a way to reduce the impact on the environment from enteric fermentation.

scholarly journals Effects of tannins and Monensin in a feedlot diet on in vitro ruminal fermentation

2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 3223
Author(s):  
Hariany Ferreira Martello ◽  
Nelcino Francisco de Paula ◽  
Bruna Gomes Macedo ◽  
Joanis Tilemahos Zervoudakis ◽  
Danielle Dias Brutti ◽  
...  
Keyword(s):  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Low Dose ◽  
Ammonia Concentration ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Kinetics Parameters ◽  
Dry Matter Digestibility

The objective of this study was to evaluate the effects of tannins versus Monensin on in vitro ruminal fermentation of a feedlot diet. The treatments were: control (no additives); low tannin (2 mg g DM-1); medium tannin (4 mg g DM-1), high tannin (6 mg g DM-1), and Monensin (0.02 mg g DM-1). The substrate was a feedlot diet composed by hay and concentrate (15:85 w/w; DM basis). Ruminal fluid was obtained from three rumen-cannulated male Santa Inês sheep. In vitro incubations were carried out during four consecutive weeks (run). Gas production (GP) was recorded at 1, 2, 3, 4, 5, 6, 8, 10, 12, 18, 24, 30, 36, 42, 48, 60, 72, 84, and 96 h of incubation. At 48 and 96 h, two bottles per treatment were withdrawn to measure pH, ammonia concentration (NH3), volatile fatty acid (VFA), in vitro dry matter digestibility (IVDMD), and in vitro neutral detergent fiber digestibility (IVNDFD). Addition of tannin or Monensin did not affect (P > 0.05) the kinetics parameters. Tannin supplementation reduced (P < 0.05) the GP at 24 h compared to Monensin. Addition of Monensin decreased (P < 0.05) IVDMD at 96 h and IVNDFD at 48 and 96 h compared to the control. The IVNDFD was lower (P < 0.05) with Monensin than with tannin at 48 and 96 h. The NH3 was lower (P < 0.05) with tannin compared with Monensin. By increasing tannin dosage, NH3 levels changed quadratically (P < 0.05). The inclusion of tannin in vitro reduced the NH3 concentration considerably when used in low dose.

scholarly journals PSVII-10 Evaluation of different biochar sources added at two inclusion levels in a grass hay- based diet on dry matter disappearance and ruminal fermentation parameters in vitro

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 296-296
Author(s):  
Genet Mengistu ◽  
Tim A McAllister ◽  
Kim Ominski ◽  
Gabriel O Ribeiro ◽  
Erasmus Okine ◽  
...  
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Buffer Solution ◽  
N Concentration ◽  
Grass Hay ◽  
Inclusion Level ◽  
The Impact

Abstract This study evaluated the impact of adding biochar to a grass hay-based diet on in vitro dry matter disappearance (DMD), total gas production (GP), methane production (CH4), volatile fatty acid (VFA) and ammonia nitrogen (NH3-N) concentration. Treatments were arranged in a factorial design with 7 biochar products differing in origin (pine or coconut-based) and physical properties (course, fine or loose spheres) at 2 inclusion levels (2.25% and 4.5% of DM) and a grass hay-only control. On each three successive weeks, rumen fluid was collected from 2 ruminally cannulated beef heifers fed a grass hay-based diet, pooled and mixed with a buffer solution (1:2) and treatments incubated in triplicate for 48 h. Gas pressure was recorded at 3, 6, 9, 12, 18, 24, 36 and 48 h of incubation, and gas was collected at each time to measure CH4. At 48 h, pH was measured and the supernatant sampled for determination of VFA and NH3-N, while the residue was used to estimate DMD. Dry matter disappearance decreased (P &lt; 0.05) with increasing addition of biochar. Biochar did not affect GP or CH4, but did reduce (P &lt; 0.05) gas production per g of DM incubated (111.7 vs 107.8 ml/g), but not per g DMD. The lower GP per g DM incubated could be related to the increased proportion of the diet as biochar, without it contributing to GP. Biochar, irrespective of level did not affect the total or molar proportions of VFA. There was a biochar x inclusion level interaction (P &lt; 0.04) in NH3-N concentration. Biochar type and inclusion level in the present study had little or no significant effect on in vitro fermentation and did not mitigate CH4 production from a grass hay-based diet.

scholarly journals The Effect of Zinc-Proteinate Supplementation on the In Vitro Digestibility and Ruminal Fermentation in Goat

2021 ◽  
pp. 180-186
Author(s):  
Gilbert Nathaniel ◽  
Tiara Annisa ◽  
Anis Muktiani ◽  
Dian Wahyu Harjanti ◽  
Widiyanto Widiyanto
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Experimental Treatment ◽  
Dairy Goat ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Organic Matter Digestibility

This study aimed primarily to investigate the effect of Zn-Proteinate (Zn-Prot) supplementation on in vitro rumen digestibility and rumen fermentation. This research used a completely randomized design with four treatments and four replicates. The experimental treatment was the supplementation of various levels of Zn-Prot (0; 12.5 ppm; 25 ppm, and 37.5 ppm) on a dry matter basis. Parameters determined were in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), partial volatile fatty acid (VFA) (acetate, propionate, and butyrate), A/P ratio, CH4, and the efficiency of the conversion hexose to VFA. Data were analyzed using ANOVA. The results showed that goats fed with a diet supplemented with 25 ppm Zn-Prot had the highest IVDMD and IVOMD values. There was no significant effect on the VFA and CH4 concentrations, A/P ratio, and the efficiency of the hexose-VFA conversion within treatment groups. In conclusion, supplementing 25 ppm of Zn-Prot into the diet of dairy goat increase the dry matter and organic matter digestibility. 

scholarly journals Effects of Additives on Silage Fermentation Characteristic and In Vitro Digestibility of Perennial Oat at Different Maturity Stages on the Qinghai Tibetan

2021 ◽  
Vol 9 (11) ◽  
pp. 2403
Author(s):  
Ping Li ◽  
Xiaolong Tang ◽  
Chaosheng Liao ◽  
Maoya Li ◽  
Liangyin Chen ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Dry Matter ◽  
Gas Production ◽  
Coliform Bacteria ◽  
In Vitro Digestibility ◽  
Flowering Stage ◽  
Ambient Temperatures ◽  
Dry Matter Digestibility ◽  
Silage Fermentation

To effectively use local grass resources to cover the winter feed shortage on the Qinghai-Tibetan Plateau, the silage fermentation and in vitro digestibility of perennial oat (Helictotrichonvirescens Henr.) were investigated. Perennial oat was harvested at the heading/flowering stage, wilted under sunny conditions, chopped, vacuumed in small bag silos, and stored at ambient temperatures (5–15 °C) for 60 days. The silages were treated without (CK) or with local lactic acid bacteria (LAB) inoculant (IN1), commercial LAB inoculant (IN2), and sodium benzoate (BL). Control silages of perennial oat at early heading stage showed higher (p < 0.05) lactate and acetate contents and lower (p < 0.05) final pH, butyrate, and ammonia-N contents than those at the flowering stage. High levels of dry matter recovery (DMR) and crude protein (CP) were observed in IN1- and BL-treated silages, with high in vitro gas production and dry matter digestibility. Compared to CK, additives increased (p < 0.05) aerobic stability by inhibiting yeasts, aerobic bacteria, and coliform bacteria during ensiling. In particular, the local LAB inoculant increased (p < 0.05) concentrations of lactate, acetate and propionate, and decreased concentrations of butyrate and ammonia-N in silages. This study confirmed that local LAB inoculant could improve the silage quality of perennial oat, and this could be a potential winter feed for animals such as yaks on the Qinghai Tibetan Plateau.

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