scholarly journals Nanosilver Application in Dental Cements

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ana Paula Rodrigues Magalhães ◽  
Laura Barbosa Santos ◽  
Lawrence Gonzaga Lopes ◽  
Cyntia Rodrigues de Araújo Estrela ◽  
Carlos Estrela ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Statistical Analysis ◽  
Streptococcus Mutans ◽  
Bactericidal Activity ◽  
Agar Plate ◽  
Antibacterial Properties ◽  
Tooth Decay ◽  
Control Groups ◽  
Made In

Streptococcus mutans is the microorganism mostly responsible for initiation of tooth decay and also for the progression of an established lesion. Silver has been used for its antibacterial properties for many years, in different forms: ionised and elementary forms, as silver zeolites or as nanoparticles. The purpose of this study was to evaluate the antibacterial activity of three dental cements modified by nanosilver. Three cements were used: Sealapex, RelyX ARC, and Vitrebond. The cements were incorporated with 0.05 mL of silver nanoparticles solution. Control groups were prepared without silver. Six Petri plates with BHI were inoculated with S. mutans using sterile swabs. Three cavities were made in each agar plate (total = 18) and filled with the manipulated cements. They were incubated at 37°C for 48 h, and the inhibition halos were measured. The paired t-Test was used for statistical analysis (P<0.05). No inhibition halos were obtained for Sealapex and Rely X, but Vitrebond showed bactericidal activity without silver and enhanced effect with silver incorporation.

scholarly journals Minireview: Formulasi Obat Kumur Ekstrak Daun Ketul (Bidens pilosa)

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Zidane Irwansyah ◽  
Hadinata Santoso ◽  
Mariana Wahjudi
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Cardiac Glycosides ◽  
Antibacterial Properties ◽  
Tween 80 ◽  
Peppermint Oil ◽  
Literature Study ◽  
Bidens Pilosa ◽  
Extraction Solvent ◽  
Water Viscosity

Abstract— Ketul (Bidens pilosa L) is weed plant that has antibacterial properties. The potential of this plant has not been developed yet in Indonesia. We conducted a literature study about this plant use for herbal mouthwash. The purposes of this study were to determine components of Ketul leaf with antibacterial activity, to know the extraction solvent, to determine the method for antibacterial activity, and to predict the characteristics and components of herbal mouthwash formula. The results showed that Ketul leaf contains tannins, cardiac glycosides, saponins, alkaloids, flavonoids, and terpenoids which could inhibit the growth of various bacteria, including Streptococcus mutans. Hence the mouthwash is predicted to have antibacterial activity. The safe extraction solvent widely used was 70-80% ethanol. The Ketul leaf extract has the potential to be applied in herbal mouthwash formula. Other characteristic can be adjusted to meet the requirements as herbal mouthwash, which are pH between 5-7, the viscosity value near water viscosity value (1cP), stable during storage, and color is brownish yellow. Some common additives were added in mouthwash to improve the flavors and taste, such as sorbitol, tween-80, peppermint oil, and sodium benzoate. Keywords: antibacterial activity, solvent, Streptococcus mutans, weed Abstrak— Ketul (Bidens pilosa L) merupakan tumbuhan gulma yang memiliki aktivitas antibakteri. Potensi tumbuhan ini belum banyak dikembangkan di Indonesia. Pada tulisan ini telah dilakukan kajian pustaka pemanfaatan Ketul untuk obat kumur herbal. Tujuan kajian ini adalah untuk mengetahui komponen ekstrak daun Ketul yang berkhasiat antibakteri, mengetahui pelarut ekstraksi, metode penentuan aktivitas antibakteri obat kumur, dan prediksi karakteristik dan komponen obat kumur herbal daun Ketul. Hasil penelusuran pustaka menunjukkan bahwa daun Ketul mengandung tannin, glikosida jantung, saponin, alkaloid, flavonoid, dan terpenoid, yang dapat menghambat pertumbuhan berbagai bakteri, termasuk Streptococcus mutans sehingga obat kumur kemungkinan memiliki aktivitas antibakteri. Pelarut aman yang banyak digunakan untuk ekstraksi komponen fitokimia tersebut adalah etanol 70-80%. Penentuan daya antibakteri dapat dilakukan menggunakan metode difusi agar atau pengenceran. Ekstrak daun Ketul berpotensi digunakan dalam formula obat kumur herbal. Selain potensi antibakterinya, karakteristik lainnya dapat diatur untuk memenuhi syarat formula obat kumur herbal yaitu pH berkisar 5-7, nilai viskositas mendekati nilai viskositas air (1cP), stabilitas dapat tetap terjaga selama penyimpanan dan kemungkinan obat kumur berwarna kuning kecoklatan. Beberapa bahan tambahan, seperti sorbitol, tween-80, peppermint oil, dan natrium benzoate, merupakan bahan tambahan yang aman dan umum digunakan untuk menambah cita rasa dan stabilitas formula obat kumur herbal ekstrak daun Ketul. Kata kunci: aktivitas antibakteri, gulma, pelarut, Streptococcus mutans

scholarly journals Dental Sealant Empowered by 1,3,5-Tri Acryloyl Hexahydro-1,3,5-Triazine and α-Tricalcium Phosphate for Anti-Caries Application

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 895 ◽  
Author(s):  
Juliana Caletti Monteiro ◽  
Michele Stürmer ◽  
Isadora Martini Garcia ◽  
Mary Anne Melo ◽  
Salvatore Sauro ◽  
...  
Keyword(s):  
Contact Angle ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Tricalcium Phosphate ◽  
Calcium Phosphates ◽  
Dental Materials ◽  
Antibacterial Properties ◽  
Chemical Properties ◽  
Knoop Hardness ◽  
Dental Sealant

: Quaternary ammonium compounds and calcium phosphates have been incorporated into dental materials to enhance their biointeractivity and preventive effects. This study aimed at evaluating the physical and chemical properties and effects against Streptococcus mutans of a dental sealant containing 1,3,5-tri acryloyl hexahydro-1,3,5-triazine (TAT) and α-tricalcium phosphate (α‐TCP). A methacrylate-based dental sealant was initially formulated. α‐TCP and TAT (Gα-TCPTAT) were added to the experimental sealant at 2 wt.% each. One group was formulated without α‐TCP and TAT and used as control (GCTRL). All tested resins were analyzed for polymerization kinetics and degree of conversion (DC %), Knoop hardness (KHN), softening in solvent (∆KHN%), ultimate tensile strength (UTS), the contact angle with water or with α-bromonaphthalene, surface free energy (SFE) and antibacterial activity against Streptococcus mutans in biofilm and in planktonic cells. The polymerization kinetic was different between groups, but without statistical differences in the DC % (p<0.05). KHN and ΔKHN% did not change between groups (p>0.05), but Gα-TCPTAT presented greater UTS compared to GCTRL (p<0.05). No differences were found for contact angle (p>0.05) or SFE (p>0.05). Gα-TCPTAT showed greater antibacterial activity in comparison to GCTRL (p<0.05). The formulation of dental sealants containing TAT and α-TCP can be characterized by improved mechanical and antibacterial properties.

scholarly journals Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on Streptococcus mutans

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 532 ◽  
Author(s):  
Barbara Lapinska ◽  
Magdalena Konieczka ◽  
Beata Zarzycka ◽  
Krzysztof Sokolowski ◽  
Janina Grzegorczyk ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Dental Materials ◽  
Antibacterial Properties ◽  
Bonding Agents ◽  
Dentin Bonding ◽  
Bonding Systems ◽  
Self Etch

There is no consensus on the antibacterial activity of dentin bonding systems (DBS). Many study models have been used to evaluate the antimicrobial activity of dental materials. In this study, a novel detection method, flow cytometry, was introduced. It allows for evaluation of the antibacterial activity of DBS, based on assessment of the disruption of the bacterial physical membrane induced by DBS. The aim of the study was to evaluate the antibacterial properties of selected dentin bonding systems against Streptococcus mutans. The highest antibacterial activity against S. mutans was observed for Adhese Universal (99.68% dead cells) and was comparable to that of Prime&Bond Universal, OptiBond Universal, or Clearfil Universal Bond Quick (p > 0.05). The lowest activity of all tested systems was displayed by the multi-mode adhesive, Universal Bond (12.68% dead bacteria cells), followed by the self-etch adhesive, OptiBond FL (15.58% dead bacteria cells). The present study showed that in the case of two-component DBS, the primer exhibited higher antimicrobial activity than the adhesive (or bond) itself.

scholarly journals Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial ActivityIn Vivo

2013 ◽  
Vol 57 (10) ◽  
pp. 4945-4955 ◽  
Author(s):  
Divya Prakash Gnanadhas ◽  
Midhun Ben Thomas ◽  
Rony Thomas ◽  
Ashok M. Raichur ◽  
Dipshikha Chakravortty
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Serum Albumin ◽  
Serum Proteins ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Resistant Bacteria ◽  
Human Society ◽  
Vis Spectroscopy

ABSTRACTThe emergence of multidrug-resistant bacteria is a global threat for human society. There exist recorded data that silver was used as an antimicrobial agent by the ancient Greeks and Romans during the 8th century. Silver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities, with minimal cytotoxic effects on the cells. However, very few reports have shown the usage of AgNPs for antibacterial therapyin vivo. In this study, we deciphered the importance of the chosen methods for synthesis and capping of AgNPs for their improved activityin vivo. The interaction of AgNPs with serum albumin has a significant effect on their antibacterial activity. It was observed that uncapped AgNPs exhibited no antibacterial activity in the presence of serum proteins, due to the interaction with bovine serum albumin (BSA), which was confirmed by UV-Vis spectroscopy. However, capped AgNPs [with citrate or poly(vinylpyrrolidone)] exhibited antibacterial properties due to minimized interactions with serum proteins. The damage in the bacterial membrane was assessed by flow cytometry, which also showed that only capped AgNPs exhibited antibacterial properties, even in the presence of BSA. In order to understand thein vivorelevance of the antibacterial activities of different AgNPs, a murine salmonellosis model was used. It was conclusively proved that AgNPs capped with citrate or PVP exhibited significant antibacterial activitiesin vivoagainstSalmonellainfection compared to uncapped AgNPs. These results clearly demonstrate the importance of capping agents and the synthesis method for AgNPs in their use as antimicrobial agents for therapeutic purposes.

scholarly journals Synthesis of Antibacterial Coating Using Chitosan, Polyethylene Glycol and Silver Nanoparticles and Investigation of Their Antibacterial Properties

2021 ◽  
Vol 13 (1) ◽  
pp. 1-9
Author(s):  
Saba Qureyshi ◽  
Zaman Sajid
Keyword(s):  
Silver Nanoparticles ◽  
Polyethylene Glycol ◽  
Agar Plate ◽  
Antibacterial Properties ◽  
Cotton Cloth ◽  
Synthesis Of Nanoparticles ◽  
Antibacterial Coating ◽  
Gram Positive Bacteria ◽  
Multiple Components ◽  
Coated Fabrics

Chitosan/ Polyethylene glycol and silver nanoparticles based antibacterial coating has been synthesized and applied to cotton cloth using sonochemical technique. In addition to the synthesis of nanoparticles using Pyrus seed extract (Green synthesis), Chitosan and PEG compound has also been used to develop the coating in this research. The coating obtained with this compound possesses good antibacterial properties and results. The study shows that the coated fabrics and silver nanoparticles show highly potent antibacterial activity towards gram negative and gram-positive bacteria. A comparison of coating with single and multiple components is studied; specifically, a comparison of pure chitosan and polyethylene glycol coating with their blend is studied. Agar plate test is performed against pseudomonas aeruginosa, Acinetobacter baumannii and methicillin-resistant staphylococcus aureus (MRSA), and the proposed process is helpful in healthcare industry and specified applications.

scholarly journals Antibacterial activity of a glass ionomer containing silver nanoparticles against Streptococcus mutans and Streptococcus sanguinis

2020 ◽  
Vol 31 (4) ◽  
pp. 589
Author(s):  
Rokhsareh Sadeghi ◽  
Hamidreza Moshfeghi ◽  
Roza Haghgoo ◽  
Mohammad Niakan ◽  
MohammadB Rezvani
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Glass Ionomer ◽  
Streptococcus Sanguinis

scholarly journals Effect of Silver Nanoparticles on Physicochemical and Antibacterial Properties of Calcium Silicate Cements

2016 ◽  
Vol 27 (5) ◽  
pp. 508-514 ◽  
Author(s):  
Fernando Vazquez-Garcia ◽  
◽  
Mário Tanomaru-Filho ◽  
Gisselle Moraima Chávez-Andrade ◽  
Roberta Bosso-Martelo ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Silver Nanoparticles ◽  
Compressive Strength ◽  
Antibacterial Activity ◽  
Calcium Silicate ◽  
Setting Time ◽  
Antibacterial Properties ◽  
Mineral Trioxide Aggregate ◽  
Biological Properties ◽  
Calcium Silicate Cements

Abstract Mineral trioxide aggregate (MTA) and Portland cement (PC) are calcium silicate cements. They have similar physicochemical, mechanical and biological properties. The addition of zirconium oxide (ZrO2) to PC provides radiopacity. Silver nanoparticles (AgNPs) may improve some properties of cements. The aim of this study was to evaluate the effect of AgNPs on physicochemical/mechanical properties and antibacterial activity of white MTA (WMTA) and PC associated with ZrO2. The following materials were evaluated: WMTA; PC 70% + ZrO2 30%; WMTA+ AgNPs; and PC 70% + ZrO2 30% + AgNPs. The study evaluated radiopacity, setting time, pH, compressive strength and solubility. For radiopacity analysis, radiographs were made alongside an aluminum (Al) step wedge. To evaluate the antibacterial activity, direct contact test was performed on planktonic cells and Enterococcus faecalis biofilm induced on bovine root dentin for 14 days. The experimental periods were 5 and 15 h. Data were obtained as CFU mL-1. The obtained data were submitted to ANOVA and Tukey tests (p<0.05). The addition of AgNPs to WMTA increased the pH, lowered the solubility and the initial and final setting times. The addition of AgNPs to PC/ZrO2 maintained the pH, lowered the solubility, and increased the setting time and compressive strength. The radiopacity of all materials was higher than 4 mmAl. The addition of AgNPs promoted an increase in antibacterial activity for calcium silicate cements and favored the physicochemical and mechanical properties of the materials.

Effect of Silver Concentration on Bioactivity and Antibacterial Properties of SiO2-CaO-P2O5 Sol-Gel Derived Bioactive Glass

2011 ◽  
Vol 493-494 ◽  
pp. 74-79 ◽  
Author(s):  
Nader Nezafati ◽  
F. Moztarzadeh ◽  
Saeed Hesaraki ◽  
Masoud Mozafari ◽  
Ali Samadikuchaksaraei ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bioactive Glass ◽  
Surface Characterization ◽  
Control Sample ◽  
Agar Plate ◽  
Antibacterial Properties ◽  
Living Environment ◽  
Molar Ratio ◽  
Human Beings ◽  
Inorganic Substances

Human beings are often infected by microorganisms in the living environment. Among various natural and inorganic substances, silver or silver ions have a powerful antibacterial activity. In the present study, the bioactivity and antibacterial activity of the SiO2–CaO–P2O5–Ag2O was compared with that of its ternary counterpart glass system (as a control sample)in vitro. The molar ratio of silver oxide in the bioactive glass composition was considered as different amount (0.5%, 1% and 2%). The surface characterization was evaluated after soaking in simulated body fluid (SBF). The sharpest apatite peak in X-ray diffraction (XRD) analyse after 7 days soaking in SBF was observed for 2% Ag sample (2%Ag-BG). Hence, the antibacterial effects of 2%Ag-BG sample against 2 gram negatives bacterium were examined by agar plate test. The result showed that the amount of silver did not prevent the HAp formation. Also, the antibacterial properties of 2%Ag-BG sample indicated near 100% bactericidal ratio (according to the width of antibacterial halo).

scholarly journals Synergistic Antibacterial Effect of Casein-AgNPs Combined with Tigecycline against Acinetobacter baumannii

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1529
Author(s):  
Yu-Hsuan Chen ◽  
Wei-Hsun Wang ◽  
Sheng-Hui Lin ◽  
Yuan-Ting Yang-Wang ◽  
Sung-Pin Tseng ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Synergistic Effect ◽  
Acinetobacter Baumannii ◽  
Nosocomial Infections ◽  
Antibacterial Effect ◽  
Combination Index ◽  
Antibacterial Properties ◽  
The Individual ◽  
Synergistic Antibacterial Activity

Acinetobacter baumannii (A. baumannii) is a common and challenging pathogen of nosocomial infections, due to its ability to survive on inanimate objects, desiccation tolerance, and resistance to disinfectants. In this study, we investigated an antibacterial strategy to combat A. baumannii via the combination of antibiotics and silver protein. This strategy used a functional platform consisting of silver nanoparticles (AgNPs) resurrected from silver-based calcium thiophosphate (SSCP) through casein and arginine. Then, the silver protein was combined with tigecycline, the first drug in glycylcycline antibiotic, to synergistically inhibit the viability of A. baumannii. The synergistic antibacterial activity was confirmed by the 96-well checkerboard method to determine their minimum inhibitory concentrations (MIC) and calculated for the combination index (CI). The MIC of the combination of silver protein and tigecycline (0.31 mg/mL, 0.16 µg/mL) was significantly lower than that of the individual MIC, and the CI was 0.59, which indicates a synergistic effect. Consequently, we integrated the detailed synergistic antibacterial properties when silver protein was combined with tigecycline. The result could make for a promising approach for the treatment of A. baumannii.

scholarly journals Green preparation and characterization of silver nanocolloids used as antibacterial material in soap

2022 ◽  
Author(s):  
M. Nowak ◽  
A. Tolińska ◽  
L. Marciniak ◽  
M. Skrobańska ◽  
B. Tylkowski ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Inductively Coupled Plasma ◽  
Antibacterial Properties ◽  
Dirty Hands ◽  
Inductively Coupled ◽  
Transmission Electron ◽  
Pure Soap ◽  
Vis Spectroscopy ◽  
Plasma Mass Spectrometry

AbstractThis study aimed to assess the characteristics, including morphology, physicochemical properties, and antibacterial properties, of silver nanocolloids obtained by D-glucose reduction. Silver nanoparticles were synthesized in accordance with the principles of green chemistry using D-glucose as a reductor. The obtained nanostructures were characterized by UV–vis spectroscopy, transmission electron microscopy, and dynamic light scattering. Stability tests performed after 1 month of storage revealed that the colloids prepared with and without polyvinylpyrrolidone as a stabilizer had the same properties. Distribution of the nanoparticles was tested using inductively coupled plasma mass spectrometry by doping the silver colloids into a natural soap mass. The antibacterial activity of the soap containing silver nanoparticles was tested on dirty hands. The antibacterial activity test demonstrated that the novel green soap materials improved with D-glucose-reduced silver nanoparticles possessed better antibacterial properties than a pure soap, and thus, they could be recommended for quotidian use by dermatological patients.

Sign in / Sign up

Export Citation Format

Share Document