scholarly journals Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on Streptococcus mutans

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 532 ◽  
Author(s):  
Barbara Lapinska ◽  
Magdalena Konieczka ◽  
Beata Zarzycka ◽  
Krzysztof Sokolowski ◽  
Janina Grzegorczyk ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Dental Materials ◽  
Antibacterial Properties ◽  
Bonding Agents ◽  
Dentin Bonding ◽  
Bonding Systems ◽  
Self Etch

There is no consensus on the antibacterial activity of dentin bonding systems (DBS). Many study models have been used to evaluate the antimicrobial activity of dental materials. In this study, a novel detection method, flow cytometry, was introduced. It allows for evaluation of the antibacterial activity of DBS, based on assessment of the disruption of the bacterial physical membrane induced by DBS. The aim of the study was to evaluate the antibacterial properties of selected dentin bonding systems against Streptococcus mutans. The highest antibacterial activity against S. mutans was observed for Adhese Universal (99.68% dead cells) and was comparable to that of Prime&Bond Universal, OptiBond Universal, or Clearfil Universal Bond Quick (p > 0.05). The lowest activity of all tested systems was displayed by the multi-mode adhesive, Universal Bond (12.68% dead bacteria cells), followed by the self-etch adhesive, OptiBond FL (15.58% dead bacteria cells). The present study showed that in the case of two-component DBS, the primer exhibited higher antimicrobial activity than the adhesive (or bond) itself.

scholarly journals Dental Sealant Empowered by 1,3,5-Tri Acryloyl Hexahydro-1,3,5-Triazine and α-Tricalcium Phosphate for Anti-Caries Application

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 895 ◽  
Author(s):  
Juliana Caletti Monteiro ◽  
Michele Stürmer ◽  
Isadora Martini Garcia ◽  
Mary Anne Melo ◽  
Salvatore Sauro ◽  
...  
Keyword(s):  
Contact Angle ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Tricalcium Phosphate ◽  
Calcium Phosphates ◽  
Dental Materials ◽  
Antibacterial Properties ◽  
Chemical Properties ◽  
Knoop Hardness ◽  
Dental Sealant

: Quaternary ammonium compounds and calcium phosphates have been incorporated into dental materials to enhance their biointeractivity and preventive effects. This study aimed at evaluating the physical and chemical properties and effects against Streptococcus mutans of a dental sealant containing 1,3,5-tri acryloyl hexahydro-1,3,5-triazine (TAT) and α-tricalcium phosphate (α‐TCP). A methacrylate-based dental sealant was initially formulated. α‐TCP and TAT (Gα-TCPTAT) were added to the experimental sealant at 2 wt.% each. One group was formulated without α‐TCP and TAT and used as control (GCTRL). All tested resins were analyzed for polymerization kinetics and degree of conversion (DC %), Knoop hardness (KHN), softening in solvent (∆KHN%), ultimate tensile strength (UTS), the contact angle with water or with α-bromonaphthalene, surface free energy (SFE) and antibacterial activity against Streptococcus mutans in biofilm and in planktonic cells. The polymerization kinetic was different between groups, but without statistical differences in the DC % (p<0.05). KHN and ΔKHN% did not change between groups (p>0.05), but Gα-TCPTAT presented greater UTS compared to GCTRL (p<0.05). No differences were found for contact angle (p>0.05) or SFE (p>0.05). Gα-TCPTAT showed greater antibacterial activity in comparison to GCTRL (p<0.05). The formulation of dental sealants containing TAT and α-TCP can be characterized by improved mechanical and antibacterial properties.

scholarly journals Morphology and thickness of the diffusion of resin through demineralized or unconditioned dentinal matrix

2002 ◽  
Vol 16 (2) ◽  
pp. 115-120 ◽  
Author(s):  
César Augusto Galvão Arrais ◽  
Marcelo Giannini
Keyword(s):  
Adhesive System ◽  
Adhesive Systems ◽  
Hybrid Layer ◽  
Human Teeth ◽  
Bonding Agents ◽  
Mean Values ◽  
Dentin Bonding ◽  
Occlusal Surfaces ◽  
The One ◽  
Bonding Systems

The formation of a hybrid layer is the main bonding mechanism of current dentin-bonding systems. This study evaluated the morphology and thickness of the resin-infiltrated dentinal layer after the application of adhesive systems. The dentin-bonding agents were evaluated on flat dentinal preparations confected on the occlusal surfaces of human teeth. The test specimens were prepared and inspected under scanning electron microscopy at a magnification of X 2,000. The adhesive systems were responsible for different hybrid layer thicknesses (p < 0.05), and the mean values were: for Scotchbond MP Plus (SM), 7.41 ± 1.24mum; for Single Bond (SB), 5.55 ± 0.82mum; for Etch & Prime 3.0 (EP), 3.86 ± 1.17mum; and for Clearfil SE Bond (CB), 1.22 ± 0.45mum. The results suggest that the conventional three-step adhesive system (SM) was responsible for the thickest hybrid layer, followed by the one-bottle adhesive (SB). The self-etching adhesives, EP and CB, produced the formation of the thinnest hybrid layers.

scholarly journals Minireview: Formulasi Obat Kumur Ekstrak Daun Ketul (Bidens pilosa)

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Zidane Irwansyah ◽  
Hadinata Santoso ◽  
Mariana Wahjudi
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Cardiac Glycosides ◽  
Antibacterial Properties ◽  
Tween 80 ◽  
Peppermint Oil ◽  
Literature Study ◽  
Bidens Pilosa ◽  
Extraction Solvent ◽  
Water Viscosity

Abstract— Ketul (Bidens pilosa L) is weed plant that has antibacterial properties. The potential of this plant has not been developed yet in Indonesia. We conducted a literature study about this plant use for herbal mouthwash. The purposes of this study were to determine components of Ketul leaf with antibacterial activity, to know the extraction solvent, to determine the method for antibacterial activity, and to predict the characteristics and components of herbal mouthwash formula. The results showed that Ketul leaf contains tannins, cardiac glycosides, saponins, alkaloids, flavonoids, and terpenoids which could inhibit the growth of various bacteria, including Streptococcus mutans. Hence the mouthwash is predicted to have antibacterial activity. The safe extraction solvent widely used was 70-80% ethanol. The Ketul leaf extract has the potential to be applied in herbal mouthwash formula. Other characteristic can be adjusted to meet the requirements as herbal mouthwash, which are pH between 5-7, the viscosity value near water viscosity value (1cP), stable during storage, and color is brownish yellow. Some common additives were added in mouthwash to improve the flavors and taste, such as sorbitol, tween-80, peppermint oil, and sodium benzoate. Keywords: antibacterial activity, solvent, Streptococcus mutans, weed Abstrak— Ketul (Bidens pilosa L) merupakan tumbuhan gulma yang memiliki aktivitas antibakteri. Potensi tumbuhan ini belum banyak dikembangkan di Indonesia. Pada tulisan ini telah dilakukan kajian pustaka pemanfaatan Ketul untuk obat kumur herbal. Tujuan kajian ini adalah untuk mengetahui komponen ekstrak daun Ketul yang berkhasiat antibakteri, mengetahui pelarut ekstraksi, metode penentuan aktivitas antibakteri obat kumur, dan prediksi karakteristik dan komponen obat kumur herbal daun Ketul. Hasil penelusuran pustaka menunjukkan bahwa daun Ketul mengandung tannin, glikosida jantung, saponin, alkaloid, flavonoid, dan terpenoid, yang dapat menghambat pertumbuhan berbagai bakteri, termasuk Streptococcus mutans sehingga obat kumur kemungkinan memiliki aktivitas antibakteri. Pelarut aman yang banyak digunakan untuk ekstraksi komponen fitokimia tersebut adalah etanol 70-80%. Penentuan daya antibakteri dapat dilakukan menggunakan metode difusi agar atau pengenceran. Ekstrak daun Ketul berpotensi digunakan dalam formula obat kumur herbal. Selain potensi antibakterinya, karakteristik lainnya dapat diatur untuk memenuhi syarat formula obat kumur herbal yaitu pH berkisar 5-7, nilai viskositas mendekati nilai viskositas air (1cP), stabilitas dapat tetap terjaga selama penyimpanan dan kemungkinan obat kumur berwarna kuning kecoklatan. Beberapa bahan tambahan, seperti sorbitol, tween-80, peppermint oil, dan natrium benzoate, merupakan bahan tambahan yang aman dan umum digunakan untuk menambah cita rasa dan stabilitas formula obat kumur herbal ekstrak daun Ketul. Kata kunci: aktivitas antibakteri, gulma, pelarut, Streptococcus mutans

Streptococcus mutans biofilm formation on chlorhexidine-containing dentin bonding agents

2013 ◽  
Vol 29 ◽  
pp. e94
Author(s):  
A. Ionescu ◽  
E. Brambilla ◽  
M. Cadenaro ◽  
F. Tay ◽  
D. Pashley ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Bonding Agents ◽  
Dentin Bonding ◽  
Dentin Bonding Agents

scholarly journals Cytocompatibility and Antibacterial Properties of Capping Materials

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Claudio Poggio ◽  
Carla Renata Arciola ◽  
Riccardo Beltrami ◽  
Annachiara Monaco ◽  
Alberto Dagna ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Disc Diffusion ◽  
Streptococcus Salivarius ◽  
Diffusion Test ◽  
Streptococcus Sanguis ◽  
Agar Disc ◽  
Bacterial Growth Inhibition

The aim of this study was to evaluate and compare the antimicrobial activity and cytocompatibility of six different pulp-capping materials: Dycal (Dentsply), Calcicur (Voco), Calcimol LC (Voco), TheraCal LC (Bisco), MTA Angelus (Angelus), and Biodentine (Septodont). To evaluate antimicrobial activity, materials were challengedin vitrowithStreptococcus mutans,Streptococcus salivarius, andStreptococcus sanguisin the agar disc diffusion test. Cytocompatibility of the assayed materials towards rat MDPC-23 cells was evaluated at different times by both MTT and apoptosis assays. Results significantly differed among the different materials tested. Both bacterial growth inhibition halos and cytocompatibility performances were significantly different among materials with different composition. MTA-based products showed lower cytotoxicity and valuable antibacterial activity, different from calcium hydroxide-based materials, which exhibited not only higher antibacterial activity but also higher cytotoxicity.

scholarly journals Immediate Dentin Bond Strength of Self-etch Dentine Adhesives

2014 ◽  
Vol 2 (1) ◽  
pp. 51
Author(s):  
David Lafuente ◽  
Isabel Ferreto
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Statistical Difference ◽  
Composite Resin ◽  
The Other ◽  
Bonding Agents ◽  
Dentin Bonding ◽  
Dentin Surface ◽  
Sixth Generation ◽  
Self Etch

Purpose: The purpose of this study was to evaluate the immediate bond strength of two sixth generation and two seventh generation dentin bonding agents to superficial dentin. Specimens were prepared to exposed superficial dentin and either Clearfil SE, Clearfil S3, Adper Prompt-l-pop of G-bond was applied over the dentin surface and light cured. Then composite resin was applied to the treated surface and light-cured in two increments. Specimens were tested 15 minutes after they were made for shear bond strength at 0.01 cm/min. Clearfil SE had statistically higher bond strength than the other three adhesives evaluated (42.9 MPa). There was no statistical difference among Clearfil S3, Adper Prompt-l-pop and G-Bond. The dentin adhesive with an application of an acidic primer before the application of the adhesive showed better immediate bond strength.

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