scholarly journals 【Original Contribution】 Characterization of Mouse Monoclonal Antibodies to Human Protein 4.1R FERM Domain: Epitope Mapping and Application to FERM Domain Binding to Red Blood Cell Inside-out Vesicles

MEMBRANE ◽  
2012 ◽  
Vol 37 (5) ◽  
pp. 250-257 ◽  
Author(s):  
Wataru Nunomura ◽  
Masahiko Hatakeyama ◽  
Yuichi Takakuwa
Keyword(s):  
Monoclonal Antibodies ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Epitope Mapping ◽  
Human Protein ◽  
Ferm Domain ◽  
Original Contribution ◽  
Protein 4.1R ◽  
Inside Out

Epitope mapping and characterization of monoclonal antibodies to human protein C

1993 ◽  
Vol 1161 (2-3) ◽  
pp. 113-123 ◽  
Author(s):  
Mikiko Takahashi ◽  
Michio Kimura ◽  
Kayoko Kurosawa-Ohsawa ◽  
Akiko Iwaki ◽  
Shigeaki Tanaka ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Protein C ◽  
Epitope Mapping ◽  
Human Protein

Detection and characterization of red blood cell (RBC) aggregation with photoacoustics

2012 ◽  
Author(s):  
Eno Hysi ◽  
Ratan K. Saha ◽  
Min Rui ◽  
Michael C. Kolios
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Rbc Aggregation

W. Dahr, in Recent Advance in Blood Group Biohchemistrv, V. Vengelen-Tyler and W.J. Judd, eds. American Association of Blood Banks, Arlington, VA (1986) pp. 23-65. 32. J-P. Cartron, in Monoclonal antibodies against human red blood cell and related antigens. P. Rouger and C. Salmon, eds. Arnette, Paris (1987) pp. 69-97. 33. D.J. Anstee, Vox Sang., 58, 1-20 (1990). 34. P. Tippett, in Blood Group Systems: Rh. V. Vengelen-Tyler and S. Pierce, eds. American Association of Blood Banks, Arlington, VA (1987) pp. 25-53 35. C. Lomas, J. Poole, N. Salaru, M. Redman, K. Kirkley, M. Moulds, J. McCreary, G.S. Nicholson, H. Hustinx and C. Green, Vox Sang., 59, 39-43 (1990). 36. J. Poole, H. Hustinx, H. Gerber, C. Lomas, Y.W. Liew, and P. Tippett, Vox Sang., 59, 44-47 (1990). 37. M. Bizot, C. Lomas, F. Rubio and P. Tippett, Transfusion, 28, 342-345 (1988). 38. N.A. Ellis, T-Z. Ye, S. Patton, J. German, P.N. Goodfellow and P. Weller, Nature Genet., 6, 394-400 (1994). 39. C. Gelin, F. Aubrit, A. Phalipon, B. Raynal, S. Cole, M. Kaczorek and A. Bernard, EMBO J., 8, 3253-3259 (1989). 40. M.N. Dworzak, G. Fritsch, P. Buchinger, C. Fleischer, D. Printz, A. Zellner, A. Schollhammer, G. Steiner, P.F. Ambros and H. Gadner, Blood, 83, 415-425 (1994). 41. R. Levy, J. Dilley, R.l. Fox and R. Warnke, Proc. Natl. Acad. Sci. USA, 76, 6552-6556 (1979). 42. G.S. Banting, B. Pym, S.M. Darling and P.N. Goodfellow, Mol Immunol., 26, 181-188 (1989). 43. P. Goodfellow, G. Banting, D. Sheer, H.H. Ropers, A. Caine, M.A. Ferguson-Smith, S. Povey and R. Voss, Nature, 302. 346-349 (1983). 44. S.M. Darling, G.S. Banting, B. Pym, J. Wolfe and P.N. Goodfellow, Proc. Natl. Acad. Sci. USA, 83, 135-139 (1986). 45. P.N. Goodfellow and P. Tippett, Nature, 289. 404-405 (1981). 46. P. Tippett, M-A. Shaw, C.A. Green and G.L. Daniels, Ann. Hum. Genet., 50, 339-347 (1986). 47. G.S. Banting, B. Pym and P.N. Goodfellow, EMBO J., 4, 1967-1972 (1985). 48. F. Latron, D. Blanchard and J-P. Cartron, Biochem. J., 247, 757-764 (1987). 49. R. Herron and G.A. Smith, Biochem. J., 262. 369-371 (1989). 50. A.C. Petty and P. Tippett Submitted.

1995 ◽  
pp. 200-205
Keyword(s):  
Monoclonal Antibodies ◽  
Blood Cell ◽  
Blood Group ◽  
Red Blood Cell ◽  
American Association ◽  
Blood Banks

scholarly journals Characterization of erythrocyte quality during the refrigerated storage of whole blood containing di-(2-ethylhexyl) phthalate

Blood ◽  
1984 ◽  
Vol 64 (6) ◽  
pp. 1270-1276 ◽  
Author(s):  
TN Estep ◽  
RA Pedersen ◽  
TJ Miller ◽  
KR Stupar
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Whole Blood ◽  
Refrigerated Storage ◽  
Cell Integrity ◽  
Normal Morphology ◽  
Plasma Hemoglobin ◽  
Induced Changes ◽  
Ethylhexyl Phthalate

Abstract Di-(2-ethylhexyl) phthalate (DEHP) accumulates in blood brought into contact with materials utilizing this compound as a plasticizer. To determine whether this phthalate diester affects red blood cell integrity, we have compared cell morphology, plasma hemoglobin accumulation, micro-vesicle production, and the concentration of intracellular metabolites and electrolytes of erythrocytes from blood stored at 4 degrees C with and without DEHP. When sufficient emulsified DEHP was mixed with blood to give a final concentration of 300 micrograms/mL, plasma hemoglobin accumulation was reduced by an average of 70%, the percentage of cells exhibiting normal morphology was enhanced by at least 20-fold, and the volume of microvesicles released from red blood cells was reduced by 50% after 35 days of refrigerated storage compared to the values obtained from corresponding samples stored without added phthalate. Similar effects were observed regardless of whether blood was stored in nonplasticized polypropylene or tri-(2-ethylhexyl) trimellitate plasticized polyvinylchloride containers and with DEHP solubilized by a variety of emulsifiers. When 300 micrograms/mL DEHP was added to stored blood containing erythrocytes predominantly in the echinocyte conformation, many of the cells reverted to the normal discoid morphology. The addition of this quantity of DEHP to blood had no significant effect on the course of storage-induced changes in erythrocyte adenosine triphosphate (ATP), 2,3-diphosphoglycerate (2,3-DPG), sodium or potassium concentrations. The data are consistent with the hypothesis that DEHP inhibits the deterioration of the red blood cell membrane that results from the refrigerated storage of whole blood.

Sign in / Sign up

Export Citation Format

Share Document