Detection and characterization of red blood cell (RBC) aggregation with photoacoustics

2012 ◽  
Author(s):  
Eno Hysi ◽  
Ratan K. Saha ◽  
Min Rui ◽  
Michael C. Kolios
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Rbc Aggregation

scholarly journals Red Blood Cell Aggregation-Associated Dietary Pattern Predicts Hyperlipidemia and Metabolic Syndrome

Nutrients ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 1127 ◽  
Author(s):  
Pei Lin ◽  
Chun-Chao Chang ◽  
Kuo-Ching Yuan ◽  
Hsing-Jung Yeh ◽  
Sheng-Uei Fang ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Dietary Patterns ◽  
Dietary Pattern ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Dietary Factors ◽  
Reduced Rank Regression ◽  
Rbc Aggregation

Red blood cell (RBC) aggregation and iron status are interrelated and strongly influenced by dietary factors, and their alterations pose a great risk of dyslipidemia and metabolic syndrome (MetS). Currently, RBC aggregation-related dietary patterns remain unclear. This study investigated the dietary patterns that were associated with RBC aggregation and their predictive effects on hyperlipidemia and MetS. Anthropometric and blood biochemical data and food frequency questionnaires were collected from 212 adults. Dietary patterns were derived using reduced rank regression from 32 food groups. Adjusted linear regression showed that hepcidin, soluble CD163, and serum transferrin saturation (%TS) independently predicted RBC aggregation (all p < 0.01). Age-, sex-, and log-transformed body mass index (BMI)-adjusted prevalence rate ratio (PRR) showed a significant positive correlation between RBC aggregation and hyperlipidemia (p-trend < 0.05). RBC aggregation and iron-related dietary pattern scores (high consumption of noodles and deep-fried foods and low intake of steamed, boiled, and raw food, dairy products, orange, red, and purple vegetables, white and light-green vegetables, seafood, and rice) were also significantly associated with hyperlipidemia (p-trend < 0.05) and MetS (p-trend = 0.01) after adjusting for age, sex, and log-transformed BMI. Our results may help dieticians develop dietary strategies for preventing dyslipidemia and MetS.

scholarly journals Characterization of erythrocyte quality during the refrigerated storage of whole blood containing di-(2-ethylhexyl) phthalate

Blood ◽  
1984 ◽  
Vol 64 (6) ◽  
pp. 1270-1276 ◽  
Author(s):  
TN Estep ◽  
RA Pedersen ◽  
TJ Miller ◽  
KR Stupar
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Whole Blood ◽  
Refrigerated Storage ◽  
Cell Integrity ◽  
Normal Morphology ◽  
Plasma Hemoglobin ◽  
Induced Changes ◽  
Ethylhexyl Phthalate

Abstract Di-(2-ethylhexyl) phthalate (DEHP) accumulates in blood brought into contact with materials utilizing this compound as a plasticizer. To determine whether this phthalate diester affects red blood cell integrity, we have compared cell morphology, plasma hemoglobin accumulation, micro-vesicle production, and the concentration of intracellular metabolites and electrolytes of erythrocytes from blood stored at 4 degrees C with and without DEHP. When sufficient emulsified DEHP was mixed with blood to give a final concentration of 300 micrograms/mL, plasma hemoglobin accumulation was reduced by an average of 70%, the percentage of cells exhibiting normal morphology was enhanced by at least 20-fold, and the volume of microvesicles released from red blood cells was reduced by 50% after 35 days of refrigerated storage compared to the values obtained from corresponding samples stored without added phthalate. Similar effects were observed regardless of whether blood was stored in nonplasticized polypropylene or tri-(2-ethylhexyl) trimellitate plasticized polyvinylchloride containers and with DEHP solubilized by a variety of emulsifiers. When 300 micrograms/mL DEHP was added to stored blood containing erythrocytes predominantly in the echinocyte conformation, many of the cells reverted to the normal discoid morphology. The addition of this quantity of DEHP to blood had no significant effect on the course of storage-induced changes in erythrocyte adenosine triphosphate (ATP), 2,3-diphosphoglycerate (2,3-DPG), sodium or potassium concentrations. The data are consistent with the hypothesis that DEHP inhibits the deterioration of the red blood cell membrane that results from the refrigerated storage of whole blood.

scholarly journals Deformability based sorting of stored red blood cells reveals donor-dependent aging curves

Lab on a Chip ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 226-235 ◽  
Author(s):  
Emel Islamzada ◽  
Kerryn Matthews ◽  
Quan Guo ◽  
Aline T. Santoso ◽  
Simon P. Duffy ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Cold Storage ◽  
Red Blood Cell ◽  
Cell Sorting ◽  
Blood Cells ◽  
Cell Deformability ◽  
Red Blood Cell Deformability

Cell sorting using microfluidic ratchets enables sensitive and consistent characterization of donor red blood cell deformability. Using this capability, we show the degradation of red blood cell deformability during cold storage is donor-dependent.

S5.5. Characterization of red blood cell mechanical properties and interactions by ultrasonic interferometry

Biorheology ◽  
1995 ◽  
Vol 32 (2-3) ◽  
pp. 125-125
Author(s):  
M RAZAVIAN ◽  
M BOYNARD ◽  
R GUILLET ◽  
H MEISELMAN ◽  
Y BEUZARD
Keyword(s):  
Mechanical Properties ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Mechanical Properties ◽  
Ultrasonic Interferometry

Modulation of endothelial nitric oxide synthase expression by red blood cell aggregation

2004 ◽  
Vol 286 (1) ◽  
pp. H222-H229 ◽  
Author(s):  
Oguz K. Baskurt ◽  
Ozlem Yalcin ◽  
Sadi Ozdem ◽  
Jonathan K. Armstrong ◽  
Herbert J. Meiselman
Keyword(s):  
Nitric Oxide ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Aggregation ◽  
Shear Stresses ◽  
Control Mechanisms ◽  
Vascular Control ◽  
Arterial Blood ◽  
Rbc Aggregation ◽  
Endothelial Nitric Oxide

The effects of enhanced red blood cell (RBC) aggregation on nitric oxide (NO)-dependent vascular control mechanisms have been investigated in a rat exchange transfusion model. RBC aggregation for cells in native plasma was increased via a novel method using RBCs covalently coated with a 13-kDa poloxamer copolymer (Pluronic F-98); control experiments used RBCs coated with a nonaggregating 8.4-kDa poloxamer (Pluronic F-68). Rats exchange transfused with aggregating RBC suspensions demonstrated significantly enhanced RBC aggregation throughout the 5-day follow-up period, with mean arterial blood pressure increasing gradually over this period. Arterial segments (≈300 μm in diameter) were isolated from gracilis muscle on the fifth day and mounted between two glass micropipettes in a special chamber equipped with pressure servo-control system. Dose-dependent dilation by ACh and flow-mediated dilation of arterial segments pressurized to 30 mmHg and preconstricted to 45–55% of the original diameter by phenylephrine were significantly blunted in rats with enhanced RBC aggregation. Both responses were totally abolished by nonspecific NO synthase (NOS) inhibitor ( Nω-nitro-l-arginine methyl ester) treatment of arterial segments, indicating that the responses were NO related. Additionally, expression of endothelial NOS protein was found to be decreased in muscle samples obtained from rats exchanged with aggregating cell suspensions. These results imply that enhanced RBC aggregation results in suppressed expression of NO synthesizing mechanisms, thereby leading to altered vasomotor tonus; the mechanisms involved most likely relate to decreased wall shear stresses due to decreased blood flow and/or increased axial accumulation of RBCs.

Effects of swimming exercise on red blood cell rheology in trained and untrained rats

2000 ◽  
Vol 88 (6) ◽  
pp. 2074-2080 ◽  
Author(s):  
Ozlem Yalcin ◽  
Melek Bor-Kucukatay ◽  
Umit K. Senturk ◽  
Oguz K. Baskurt
Keyword(s):  
Lipid Peroxidation ◽  
Blood Cell ◽  
Transit Time ◽  
Red Blood Cell ◽  
Swimming Exercise ◽  
Aggregation Index ◽  
Control Value ◽  
Cell Rheology ◽  
Transient Impairment ◽  
Rbc Aggregation

Red blood cell (RBC) mechanical properties were investigated after swimming exercise in trained and untrained rats. A group of rats was trained for 6 wk (60 min swimming, daily), and another group was kept sedentary. Blood samples were obtained either within 5 min or 24 h after 60 min swimming in both groups. In the untrained rats, the RBC aggregation index decreased to 2.60 ± 0.4 immediately after exercise from a control value of 6.73 ± 0.18 ( P < 0.01), whereas it increased to 13.13 ± 0.66 after 24 h ( P < 0.01). RBC transit time through 5-μm pores increased to 3.53 ± 0.16 ms within 5 min after the exercise from a control value of 2.19 ± 0.07 ms ( P < 0.005). A very significant enhancement (166%) in RBC lipid peroxidation was detected only after 24 h. In the trained group, the alterations in all these parameters were attenuated; there was a slight, transient impairment in RBC deformability (transit time = 2.64 ± 0.13 ms), and lipid peroxidation was found to be unchanged. These findings suggest that training can significantly limit the hemorheological alterations related to a given bout of exercise. Whether this effect is secondary to the training-induced reduction in the degree of metabolic and/or hormonal perturbation remains to be determined.

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