scholarly journals Validation and Calibration of an Electrical Analog Model of Human Liver Perfusion Based on Hypothermic Machine Perfusion Experiments

2014 ◽  
Vol 37 (6) ◽  
pp. 486-498 ◽  
Author(s):  
Charlotte Debbaut ◽  
Diethard R.L. Monbaliu ◽  
Patrick Segers
Keyword(s):  
Human Liver ◽  
Liver Perfusion ◽  
Machine Perfusion ◽  
Analog Model ◽  
Electrical Analog ◽  
Hypothermic Machine Perfusion

From Human Liver Vascular Corrosion Cast to Electrical Analog Model of the Hepatic Blood Flow

2010 ◽  
Author(s):  
C. Debbaut ◽  
D. Monbaliu ◽  
C. Casteleyn ◽  
P. Cornillie ◽  
D. Van Loo ◽  
...  
Keyword(s):  
Blood Flow ◽  
Organ Preservation ◽  
Human Liver ◽  
Machine Perfusion ◽  
Analog Model ◽  
Hepatic Microcirculation ◽  
Electrical Analog ◽  
Expanded Criteria ◽  
Corrosion Cast ◽  
Potential Damage

Machine perfusion (MP) is experiencing a revival in organ preservation due to the limitations of static cold storage and the need for better preservation methods for expanded criteria donors. Liver MP prototypes, however, face problems such as potential damage to sinusoidal endothelial cells, and heterogeneous perfusion related to the complex hepatic microcirculation.

From Vascular Corrosion Cast to Electrical Analog Model for the Study of Human Liver Hemodynamics and Perfusion

2011 ◽  
Vol 58 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Charlotte Debbaut ◽  
Diethard Monbaliu ◽  
Christophe Casteleyn ◽  
Pieter Cornillie ◽  
Denis Van Loo ◽  
...  
Keyword(s):  
Human Liver ◽  
Analog Model ◽  
Electrical Analog ◽  
Vascular Corrosion Cast ◽  
Corrosion Cast ◽  
Liver Hemodynamics

scholarly journals Optimizing porcine donor kidney preservation with normothermic or hypothermic machine perfusion: A systematic review

2021 ◽  
Author(s):  
Sarah Bouari ◽  
Özgür Eryigit ◽  
Ron W. F. Bruin ◽  
Jan N. M. IJzermans ◽  
Robert C. Minnee
Keyword(s):  
Systematic Review ◽  
Machine Perfusion ◽  
Donor Kidney ◽  
Kidney Preservation ◽  
Hypothermic Machine Perfusion

scholarly journals Human liver stem cell‐derived extracellular vesicles reduce injury in a model of normothermic machine perfusion of rat livers previously exposed to a prolonged warm ischemia

2021 ◽  
Vol 34 (9) ◽  
pp. 1607-1617
Author(s):  
Nicola De Stefano ◽  
Victor Navarro‐Tableros ◽  
Dorotea Roggio ◽  
Alberto Calleri ◽  
Federica Rigo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Extracellular Vesicles ◽  
Human Liver ◽  
Warm Ischemia ◽  
Machine Perfusion ◽  
Normothermic Machine Perfusion ◽  
Rat Livers

scholarly journals Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion

10.3791/52777 ◽  
2015 ◽  
Author(s):  
Bote G. Bruinsma ◽  
James H. Avruch ◽  
Pepijn D. Weeder ◽  
Gautham V. Sridharan ◽  
Basak E. Uygun ◽  
...  
Keyword(s):  
Human Liver ◽  
Machine Perfusion ◽  
Liver Preservation

Primary Human Liver Cells as Source for Modular Extracorporeal Liver Support - a Preliminary Report

2002 ◽  
Vol 25 (10) ◽  
pp. 1001-1005 ◽  
Author(s):  
I.M. Sauer ◽  
K. Zeilinger ◽  
N. Obermayer ◽  
G. Pless ◽  
A. Grünwald ◽  
...  
Keyword(s):  
Human Liver ◽  
Treatment Time ◽  
Liver Perfusion ◽  
Cell Mass ◽  
Liver Weight ◽  
Liver Cells ◽  
Liver Support ◽  
Overall Treatment Time ◽  
Human Liver Cells ◽  
Extracorporeal Liver Support

Cell-based extracorporeal liver support is an option to assist or replace the failing organ until regeneration or until transplantation can be performed. The use of porcine cells or tumor cell lines is controversial. Primary human liver cells, obtained from explanted organs found to be unsuitable for transplantation, are a desirable cell source as they perform human metabolism and regulation. The Modular Extracorporeal Liver Support (MELS) concept combines different extracorporeal therapy units, tailored to suit the individual and intra-individual clinical needs of the patient. A multi-compartment bioreactor (CellModule) is loaded with human liver cells obtained by 5-step collagenase liver perfusion. A cell mass of 400 g – 600 g enables the clinical application of a liver lobe equivalent hybrid organ. A detoxification module enables single pass albumin-dialysis via a standard high-flux dialysis filter, and continuous venovenuous hemodiafiltration may be included if required. Cells from 54 human livers have been isolated (donor age: 56 ± 13 years, liver weight: 1862 ± 556 g resulting in a viability of 55.0 ± 15.9%). These grafts were not suitable for LTx, due to steatosis (54%), cirrhosis (15%), fibrosis (9%), and other reasons (22%). Out of 36 prepared bioreactors, 10 were clinically used to treat 8 patients with liver failure. The overall treatment time was 7–144 hours. No adverse events were observed. Initial clinical applications of the bioreactor evidenced the technical feasibility and safety of the system.

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