Ischemic postconditioning improves the outcome of organs from donors after cardiac death in a pig liver transplantation model and provides synergistic protection with hypothermic machine perfusion

2021 ◽  
Author(s):  
Degong Jia ◽  
Qi Pan ◽  
Yijie Zhang ◽  
Yang Yu ◽  
Zhanyu Song ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Cardiac Death ◽  
Ischemic Postconditioning ◽  
Machine Perfusion ◽  
Pig Liver ◽  
Hypothermic Machine Perfusion ◽  
Transplantation Model

scholarly journals Effects of SP600125 and hypothermic machine perfusion on livers donated after cardiac death in a pig allograft transplantation model

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Yijie Zhang ◽  
Qi Pan ◽  
Ying Cheng ◽  
Yongfeng Liu
Keyword(s):  
Liver Transplantation ◽  
Survival Rate ◽  
Cardiac Death ◽  
Ischemia Reperfusion ◽  
Donation After Cardiac Death ◽  
Machine Perfusion ◽  
Serological Examination ◽  
Satisfactory Outcome ◽  
Hypothermic Machine Perfusion

Abstract Background Hypothermic machine perfusion (HMP) improves the quality of donor livers for transplantation, both in animal models and in clinical practice. Treatment with SP600125, an inhibitor of c-Jun N-terminal kinase (JNK), can suppress the JNK signaling pathway to alleviate donor liver ischemia–reperfusion injury (IRI). We performed the present study with the objective of exploring the protective effects exerted by a combination of HMP and SP600125 on liver xenograft viability for donation after cardiac death (DCD) in a porcine model. Methods 54 adult BAMA mini-pigs were randomly assigned to 5 groups, including sham, cold storage for 4 h (CS 4 h), CS 4 h + SP600125, CS 2 h + HMP 2 h, and CS 2 h + HMP 2 h + SP600125 groups. Donor livers in the CS 4 h and CS 4 h + SP600125 groups were conventionally cold preserved for 4 h, whereas donor livers in the CS 2 h + HMP 2 h and CS 2 h + HMP 2 h + SP600125 groups were cold preserved for 2 h and then treated with HMP for 2 h. The preservation and perfusion solutions contained SP600125 (20 µM). Follow-up was conducted for 5 days after liver transplantation to compare the surgical outcomes by means of serological examination, pathological results, and survival rate. Results The most satisfactory outcome after liver transplantation was observed in the CS 2 h + HMP 2 h + SP600125 group, which presented with minimal damage of donor livers during 5 days’ follow-up. Additionally, serological examination, pathological results, and survival rate concurred in showing better results in the CS 2 h + HMP 2 h ± SP600125 group than in the CS 4 h ± SP600125 group. Conclusion HMP in combination with SP600125 has hepatoprotective properties and improves the quality and viability of porcine livers collected after DCD, thus improving prognosis after liver transplantation.

scholarly journals Evaluation Using an Isolated Reperfusion Model for Porcine Liver Donated After Cardiac Death Preserved with Oxygenated Hypothermic Machine Perfusion

2018 ◽  
Vol 23 ◽  
pp. 822-827 ◽  
Author(s):  
Ryo Yoshikawa ◽  
Naoto Matsuno ◽  
Noriyuki Morito ◽  
Mikako Gouchi ◽  
Masahide Otani ◽  
...  
Keyword(s):  
Cardiac Death ◽  
Machine Perfusion ◽  
Porcine Liver ◽  
Hypothermic Machine Perfusion

scholarly journals Dual machine perfusion via hepatic artery and portal vein in mouse liver transplantation model

HPB ◽  
2016 ◽  
Vol 18 ◽  
pp. e545
Author(s):  
M. Fujiyoshi ◽  
S. Fujiyoshi ◽  
M. Fukai ◽  
R. Porte ◽  
A. Taketomi
Keyword(s):  
Liver Transplantation ◽  
Portal Vein ◽  
Hepatic Artery ◽  
Mouse Liver ◽  
Machine Perfusion ◽  
Transplantation Model

Reconditioning of Lungs Donated after Cardiac Death with Short-Term Hypothermic Machine Perfusion

2012 ◽  
Vol 94 (10S) ◽  
pp. 39
Author(s):  
D. Nakajima ◽  
F. Chen ◽  
K. Okita ◽  
H. Motoyama ◽  
K. Hijiya ◽  
...  
Keyword(s):  
Cardiac Death ◽  
Machine Perfusion ◽  
Short Term ◽  
Hypothermic Machine Perfusion
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