From Vascular Corrosion Cast to Electrical Analog Model for the Study of Human Liver Hemodynamics and Perfusion

2011 ◽  
Vol 58 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Charlotte Debbaut ◽  
Diethard Monbaliu ◽  
Christophe Casteleyn ◽  
Pieter Cornillie ◽  
Denis Van Loo ◽  
...  
Keyword(s):  
Human Liver ◽  
Analog Model ◽  
Electrical Analog ◽  
Vascular Corrosion Cast ◽  
Corrosion Cast ◽  
Liver Hemodynamics

From Human Liver Vascular Corrosion Cast to Electrical Analog Model of the Hepatic Blood Flow

2010 ◽  
Author(s):  
C. Debbaut ◽  
D. Monbaliu ◽  
C. Casteleyn ◽  
P. Cornillie ◽  
D. Van Loo ◽  
...  
Keyword(s):  
Blood Flow ◽  
Organ Preservation ◽  
Human Liver ◽  
Machine Perfusion ◽  
Analog Model ◽  
Hepatic Microcirculation ◽  
Electrical Analog ◽  
Expanded Criteria ◽  
Corrosion Cast ◽  
Potential Damage

Machine perfusion (MP) is experiencing a revival in organ preservation due to the limitations of static cold storage and the need for better preservation methods for expanded criteria donors. Liver MP prototypes, however, face problems such as potential damage to sinusoidal endothelial cells, and heterogeneous perfusion related to the complex hepatic microcirculation.

A CORROSION CAST STUDY OF RAMIFICATION PATTERN OF PORTAL VEIN IN RIGHT LOBE OF HUMAN LIVER

2014 ◽  
Vol 2 (4) ◽  
pp. 791-796 ◽  
Author(s):  
Rajput AS ◽  
◽  
Kumari S ◽  
Mishra GP ◽  
◽  
...  
Keyword(s):  
Portal Vein ◽  
Human Liver ◽  
Right Lobe ◽  
Corrosion Cast

Linear relation between time constant of oxygen uptake kinetics, total creatine, and mitochondrial content in vitro

2008 ◽  
Vol 294 (1) ◽  
pp. C79-C87 ◽  
Author(s):  
Brian Glancy ◽  
Thomas Barstow ◽  
Wayne T. Willis
Keyword(s):  
Time Constant ◽  
Mitochondrial Protein ◽  
Oxygen Uptake Kinetics ◽  
Analog Model ◽  
Electrical Analog ◽  
Skeletal Muscle Mitochondria ◽  
Rate Of Oxygen Consumption ◽  
Total Creatine ◽  
Kinetics Of

Following the onset of moderate aerobic exercise, the rate of oxygen consumption (Jo) rises monoexponentially toward the new steady state with a time constant (τ) in the vicinity of 30 s. The mechanisms underlying this delay have been studied over several decades. Meyer's electrical analog model proposed the concept that the τ is given by τ = Rm· C, where Rm is mitochondrial resistance to energy transfer, and C is metabolic capacitance, determined primarily by the cellular total creatine pool (TCr = phosphocreatine + creatine). The purpose of this study was to evaluate in vitro the Jo kinetics of isolated rat skeletal muscle mitochondria at various levels of TCr and mitochondrial protein. Mitochondria were incubated in a medium containing 5.0 mM ATP, TCr pools of 0–1.5 mM, excess creatine kinase, and an ATP-splitting system of glucose + hexokinase (HK). Pyruvate and malate (1 mM each) were present as oxidative substrates. Jo was measured across time after HK was added to elicit one of two levels of Jo (40 and 60% of state 3). At TCr levels (in mM) of 0.1, 0.2, 0.3, 0.75, and 1.5, the corresponding τ values (s, means ± SE) were 22.2 ± 3.0, 36.3 ± 2.2, 65.7 ± 4.3, 168.1 ± 22.2, and 287.3 ± 25.9. Thus τ increased linearly with TCr ( R2 = 0.916). Furthermore, the experimentally observed τ varied linearly and inversely with the mitochondrial protein added. These in vitro results consistently conform to the predictions of Meyer's electrical analog model.

scholarly journals Effects of curcumin on testis microvascular complication in streptozotocin-induced diabetes rats

2019 ◽  
Vol 9 (1) ◽  
pp. 34
Author(s):  
Wipapan Khimmaktong ◽  
Manaras Komolkriengkrai ◽  
Piyakorn Boonyoung ◽  
Vipavee Anupunpisit
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Structural Changes ◽  
Testicular Tissue ◽  
Diabetic Rats ◽  
Seminiferous Tubules ◽  
Vascular Corrosion Cast ◽  
Pampiniform Plexus ◽  
Corrosion Cast ◽  
Scanning Electron

Background:  Diabetes has a significant impact on the impaired function of the reproductive system in the testis.Objective: The aim of this study was to investigate the effects of curcumin from turmeric on structural changes of testicular tissues and microvasculature in STZ-induced diabetic rats.Methods: Rats were injected with a single dose of streptozotocin (60 mg/kg BW) to induce a diabetic condition. Three days after the injection, rats with a blood sugar level > 250 mg/dl were considered diabetic. Rats were allocated to one of three groups, diabetic (DM), diabetic rats receiving curcumin (DMC) (200 mg/kg BW), and control (C). Testicular tissues were studied eight weeks after the treatment using the histological technique. Testicular microvasculature was examined using the vascular corrosion cast technique under a scanning electron microscope (SEM).Results: The testicular tissue of DM group showed damaged seminiferous tubules, spermatocytes, and spermatids with necrosis and germ cells degeneration. Destruction of testicular arteries and veins in pampiniform plexus were demonstrated. The destroyed capillaries, arterioles, and venules demonstrated stenosis and shrinkage. The vessel diameter in all DM groups decreased. In the DMC group which received curcumin, the appearance of all seminiferous tubules, spermatogenic, and sertoli cell was normal. The reconstruction and restoration of testicular artery, pampiniform plexus, capillary network, arterioles, and venules were clear. As a result of curcumin supplementation, the standard and healthy testis microvasculature was restored. The DMC vessels diameter increased back to a quasi normal condition, particularly at the artery, arteriole, and capillary levels.Conclusions: The results provided evident for effects of curcumin could be associated with recovering and improvement of the testicular tissues and microvasculatures in diabetic condition.Keywords: Diabetes mellitus, Streptozotocin, Curcumin, Testis, Microvasculature, Vascular corrosion cast, Scanning electron microscope

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