Screening of cultivation media for ldpe biodegradation by Penicillium verrucosum CNM-FP-02

2021 ◽  
Author(s):  
Olga Postolachi ◽  
◽  
Inna Rastimesina ◽  
Valentina Josan ◽  
Vera Mamaliga ◽  
...  
Keyword(s):  
Penicillium Verrucosum

The Mycobiota of Speck, a Traditional Tyrolean Smoked and Cured Ham

2000 ◽  
Vol 63 (10) ◽  
pp. 1399-1403 ◽  
Author(s):  
URSULA PEINTNER ◽  
JOHANNES GEIGER ◽  
REINHOLD PÖDER
Keyword(s):  
Species Diversity ◽  
Fungal Species ◽  
Penicillium Canescens ◽  
Penicillium Verrucosum ◽  
South Tyrol ◽  
Cured Meat ◽  
Penicillium Commune ◽  
Penicillium Solitum ◽  
Penicillium Spp

Speck is a ham specialty product traditionally produced in South Tyrol (Italy) and North Tyrol (Austria) by farmers, butcheries, and meat industries. To date, nothing has been learned about fungi associated with this smoked and cured meat. Therefore, it was the main objective of this study to assess the typical mycobiota of Speck in relation to the different production types and the geographic provenance. A total of 121 Speck samples from North Tyrol and South Tyrol was analyzed. From 63 isolated fungal species, only a few can be regarded as typical colonizers: Eurotium rubrum and Penicillium solitum were the dominating species in all types and parts of Speck (crust, meat, and fat). Eight other Penicillium spp. were relatively frequent. The species diversity increased from industrially produced Speck to products from butcheries and farmers, and it was higher in all types of South Tyrolean products. Among the typical mycobiota, Penicillium verrucosum, Penicillium canescens, and Penicillium commune are known as potentially mycotoxigenic.

scholarly journals Analysis of Fungal Contamination on Commercially Sold Rice in Puerto Rico

2018 ◽  
Vol 15 (2) ◽  
Author(s):  
Nicole Colón Carrión ◽  
Chad Lozada Troche
Keyword(s):  
Rice Variety ◽  
Fungal Endophytes ◽  
Brown Rice ◽  
P Value ◽  
Rice Grain ◽  
Penicillium Verrucosum ◽  
Normal Surface ◽  
White Rice ◽  
Rice Grains ◽  
Fungal Contaminants

Crops and stored grains are susceptible to pathogens that represent a threat to our health. The study presented herein compares the normal surface and endophytic fungal communities present on white and brown rice grains. One hundred grains of each rice variety was analyzed to determine their fungal contaminants and endophytes. Fungi were inoculated on SDA media, and purified in PDA media; morphological characterization was performed followed by amplification of the ITS region using PCR for all fungal isolates. Statistical analysis indicated significant differences between medium brown rice compared to white rice for surface and endophytic communities (p-value £ 0.05). In addition, a higher fungal diversity was found on brown rice grains compared to white rice. This variation may be due to differences in the processing methods used for each rice grain type. BLAST analysis revealed the presence of toxigenic strains of Aspergillus flavus, A.oryzae, Penicillium verrucosum, and P. viridicatum. The study of fungal growth in rice grains can contribute to the minimization of mycotoxin production by its prevention and control; therefore, decreasing crop contamination and human exposure to their metabolites. KEYWORDS: Fungi; Rice; Fungal contaminants; Fungal endophytes

scholarly journals Development of Biodegradable Whey-Based Laminate Functionalised by Chitosan–Natural Extract Formulations

2020 ◽  
Vol 21 (10) ◽  
pp. 3668
Author(s):  
Sanja Potrč ◽  
Lidija Fras Zemljič ◽  
Meta Sterniša ◽  
Sonja Smole Možina ◽  
Olivija Plohl
Keyword(s):  
Antimicrobial Activity ◽  
Oxygen Permeability ◽  
Surface Composition ◽  
Antioxidant Properties ◽  
Chitosan Nanoparticles ◽  
Barrier Properties ◽  
Penicillium Verrucosum ◽  
Natural Extracts ◽  
Positive Effect ◽  
Macromolecular Solution

In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside. The aim was to establish the biodegradable and active concept of packaging laminates. For this purpose, chitosan nanoparticles (CSNPs) with embedded rosemary or cinnamon extracts were synthesised and characterised. Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition). Such functionalised whey-based laminate was physicochemically characterized in terms of elemental surface composition, wettability, morphology and oxygen permeability. The antimicrobial activity was tested against Staphylococcus aureus, Escherichia coli, Aspergillus flavus and Penicillium verrucosum. The antioxidant properties were determined using the ABTS assay. It could be shown that after functionalization of the films with the above-mentioned strategy, the wettability was improved. Furthermore, such whey-based laminates still show excellent barrier properties, good antimicrobial activity and a remarkable antioxidative activity. In addition to the improved biodegradability, this type of lamination could also have a positive effect on the shelf-life of products packaged in such structured films.

scholarly journals Effect of fungicides on the growth of fungi isolated from in vitro propagated fruit-bearing plants

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 329-331
Author(s):  
M. Kowalik ◽  
M. Gródek
Keyword(s):  
In Vitro Propagation ◽  
Linear Growth ◽  
Linear Growth Rate ◽  
Ms Medium ◽  
Penicillium Verrucosum ◽  
Mortierella Isabellina ◽  
Cladosporium Sphaerospermum ◽  
Murashige Skoog ◽  
Growth Of Fungi

The aim of the work was to determine the effect of fungicides: Topsin M 70 WG, Bravo 500 SC, and Sportak 45 EC in two concentrations on the growth of fungi: Aspergillus niger, Epicoccum purpurascens, Cladosporium sphaerospermum, Mortierella isabellina and Penicillium verrucosum var. verrucosum on PDA and Murashige-Skoog medium (MS). The smallest index of linear growth rate of the fungi had Topsin M 70 WG for fungi: E. purpurascens and P. verrucosum var. verrucosum on PDA and MS medium and for M. isabellina and C. sphaerospermum on MS in concentrations of 71.42 mg/l and 107.13 mg/l. The highest index of linear growth had Bravo 500 SC in concentrations of 0.5 ml/l and 0.75 ml/l on PDA for E. purpurascens and A. niger. The lowest index of linear growth on MS for E. purpurascens, C. sphaerospermum and P. verrucosum var. verrucosum was noted down. The highest effectiveness in control of linear growth all tested species of fungi was found for fungicide Sportak 45 EC in concentration of 0.55 ml/l and 0.825 ml/l on MS and PDA. Among all tested fungicides used in vitro propagation fungicides from imidazoles group are recommended.

Real time PCR: a good tool to estimate mycotoxin contamination in pig diets

2017 ◽  
Vol 10 (3) ◽  
pp. 219-228 ◽  
Author(s):  
A. Okorski ◽  
M. Polak-Śliwińska ◽  
K. Karpiesiuk ◽  
A. Pszczółkowska ◽  
W. Kozera
Keyword(s):  
Soybean Meal ◽  
Animal Health ◽  
Aspergillus Ochraceus ◽  
Transgenic Soybean ◽  
Penicillium Verrucosum ◽  
Fusarium Spp ◽  
Potential Threat ◽  
Good Tool ◽  
Percentage Content ◽  
Mycotoxin Contamination

Cereals and soybean are the main components of pig diets. Unfortunately, feed materials are often contaminated with fungi and their metabolites, which pose a potential threat to human and animal health. Therefore, this study was undertaken to evaluate the effectiveness of cultural methods and quantitative PCR for detecting fungi and their metabolites in pig diets, and to determine which plant components are responsible for mycotoxin contamination of feed. The presence of mycotoxin-producing fungi of the genera Fusarium, Penicillium and Aspergillus and their metabolites was determined in pig diets with different inclusion levels of various cereals and transgenic soybean meal. Six farm-made complete diets containing locally produced feed materials and imported soybean meal were investigated. The presence of the following fungi in pig diets was determined by microscopic observations of fungal cultures and by qPCR: trichothecene-producing Fusarium spp. (Tri5 gene), Penicillium verrucosum (rRNA) and Aspergillus ochraceus (PKS gene). The concentrations of mycotoxins (ochratoxin A (OTA) and zearalenone (ZEA)), trichothecenes (deoxynivalenol (DON), 3-acetyl-deoxynivalenol and T-2 toxin (T-2)) were analysed by HPLC. The results of the qPCR analysis demonstrated that the presence of DNA of mycotoxin-producing fungi and mycotoxins in pig diets was correlated with the inclusion levels of transgenic soybean meal and various cereals. The above correlation was validated by an analysis of Spearman’s rank correlation between the content of transgenic soybean meal and various cereals vs mycotoxin concentrations and the amount of DNA of toxin-producing fungi in pig diets. A significant positive correlation was found between: the percentage content of soybeans vs the concentrations of DON (R=0.93), trichothecenes (R=0.76) and T-2 (R=0.64), the percentage content of barley vs the concentrations of DON (R=0.50) and T-2 (R=0.49), the percentage content of triticale vs OTA levels (R=0.47), the percentage content of oats vs ZEA levels (0.50). A correlation was also noted between the percentage content of soybeans and the amount of DNA of trichothecene-producing Fusarium spp. (R=0.96). The results of this study indicate that pig diets are significantly contaminated with toxin-producing fungi and their metabolites, and that the quantification of DNA of mycotoxin-producing fungi is a reliable indicator of mycotoxin contamination of feed. Our findings can contribute to reducing the costs of analyses that should be routinely performed to minimise the entry of mycotoxins into the food chain.

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