Environmental factors affect efficacy of some essential oils and resveratrol to control growth and ochratoxin A production by Penicillium verrucosum and Aspergillus westerdijkiae on wheat grain

2008 ◽  
Vol 44 (4) ◽  
pp. 341-346 ◽  
Author(s):  
David Aldred ◽  
Victoria Cairns-Fuller ◽  
Naresh Magan
Keyword(s):  
Environmental Factors ◽  
Essential Oils ◽  
Ochratoxin A ◽  
Wheat Grain ◽  
Penicillium Verrucosum ◽  
Control Growth ◽  
Aspergillus Westerdijkiae

scholarly journals Effects of selected essential oils on the growth and production of ochratoxin A by Penicillium verrucosum

2014 ◽  
Vol 65 (2) ◽  
pp. 199-208 ◽  
Author(s):  
Barbara Jeršek ◽  
Nataša Poklar Ulrih ◽  
Mihaela Skrt ◽  
Neda Gavarić ◽  
Biljana Božin ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Ochratoxin A ◽  
Abies Alba ◽  
Inhibitory Effect ◽  
Mentha Piperita ◽  
Penicillium Verrucosum ◽  
Foeniculum Vulgare ◽  
Oregano Essential Oil ◽  
Minimal Inhibitory Concentrations

AbstractEssential oils from oregano (Origanum vulgare L.), mint (Mentha piperita L.), fennel (Foeniculum vulgare Mill.), and pine (Abies alba Mill.) needles and cones, and their active substances thymol, carvacrol, menthol, and anisaldehyde were tested for antifungal activity against Penicillium verrucosum. The lowest minimal inhibitory concentrations (MICs) were achieved for essential oil of oregano, followed by carvacrol, thymol, and menthol. These antifungal components were further investigated, as the main aim of our study was to elucidate the effect of natural antifungals on ochratoxin A production. During 21 days of exposure, the growth of P. verrucosum, and subsequently the production of ochratoxin A, was fully inhibited by thymol at ½ MIC (0.0625 mg mL-1), but menthol at ¼ and ½ MIC (0.1875 and 3750 mg mL-1) showed no growth inhibition. After 21 days of incubation, the greatest inhibitory effect on ochratoxin production (inhibition was 96.9 %) was also achieved with thymol at ¼ MIC (0.0313 mg mL-1). Essential oil of oregano (¼ MIC, 0.2930 μL mL-1) and carvacrol (½ MIC, 0.1953 μL mL-1) stimulate production of ochratoxin A at 13.9 % to 28.8 %, respectively. The observed antifungal effects depended on the agent, the concentration used, and the time of interaction between the agent and P. verrucosum. Our results indicate the possibility of using oregano essential oil as a substitute for artificial preservatives in certain foods, but further research is needed.

scholarly journals Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by Aspergillus westerdijkiae and Penicillium verrucosum

2020 ◽  
Vol 21 (22) ◽  
pp. 8548
Author(s):  
Saranyaphat Boonmee ◽  
Vessela Atanasova ◽  
Sylvain Chéreau ◽  
Gisèle Marchegay ◽  
Kevin D. Hyde ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Fungal Growth ◽  
Culture Conditions ◽  
Growth Reduction ◽  
Penicillium Verrucosum ◽  
Aspergillus Westerdijkiae ◽  
Synthetic Pesticides ◽  
Definition Of ◽  
The Impact ◽  
Potential Use

Ochratoxin A (OTA) is one of the worldwide most important mycotoxins in terms of health and agroeconomic consequences. With the aim to promote the use of phytochemicals as alternatives to synthetic fungicides, the effect of hydroxycinnamic acids on the fungal growth and OTA yield by two major OTA-producing species was investigated. After a first step dedicated to the definition of most suitable culture conditions, the impact of 0.5 mM ferulic (FER), p-coumaric (COUM), caffeic and chlorogenic acids was evaluated on Aspergillus westerdijkiae and Penicillium verrucosum. Whereas no fungal growth reduction was observed regardless of the phenolic acid and fungal isolate, our results demonstrated the capacity of FER and COUM to inhibit OTA production. The most efficient compound was FER that led to a 70% reduction of OTA yielded by P. verrucosum and, although not statistically significant, a 35% inhibition of OTA produced by A. westerdijkiae. To further investigate the bioactivity of FER and COUM, their metabolic fate was characterized in fungal broths. The capacity of P. verrucosum to metabolize FER and COUM through a C2-clivage type degradation was demonstrated. Overall, our data support the potential use of FER to prevent OTA contamination and reduce the use of synthetic pesticides.

Agrobacterium-mediated insertional mutagenesis of the ochratoxigenic fungus Aspergillus westerdijkiae

2007 ◽  
Vol 53 (1) ◽  
pp. 148-151 ◽  
Author(s):  
Marcia M Mata ◽  
Marta H Taniwaki ◽  
Beatriz T Iamanaka ◽  
Daniele Sartori ◽  
André L.M Oliveira ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Insertional Mutagenesis ◽  
Aspergillus Ochraceus ◽  
Agrobacterium Mediated Transformation ◽  
Morphological Variations ◽  
Carcinogenic Potential ◽  
Coffee Beans ◽  
Aspergillus Westerdijkiae ◽  
Polymerase Chain ◽  
First Time

Aspergillus westerdijkiae is a potent ochratoxin A (OTA) producer that has been found in coffee beans. OTA is known to have nephrotoxic effects and carcinogenic potential in animal species. Here we report for the first time the Agrobacterium-mediated transformation for Aspergillus westerdijkiae and the generation of ochratoxin-defective mutants. Conidia were transformed to hygromycin B resistance using strain AGL-1 of Agrobacterium tumefaciens. The obtained transformation frequency was up to 47 transformants per 106 target conidia. Among 600 transformants, approximately 5% showed morphological variations. Eight transformants with consistently reduced OTA production were obtained. Two of these transformants did not produce OTA (detection limit: 0.1 µg/kg); the other six mutants produced lower amounts of OTA (1%–32%) compared with the wild-type strain. By using thermal asymmetric interlaced polymerase chain reaction, we successfully identified a putative flavin adenine dinucleotide monooxygenase gene.Key words: Aspergillus ochraceus, Aspergillus westerdijkiae, Agrobacterium-mediated transformation, Agrobacterium-mediated insertional mutagenesis, ochratoxin A.

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