Direct Analysis of Elastomer Compounds by Soft Ionization, Tandem (MS/MS) and High Resolution (AC-MS) Mass Spectrometry

1995 ◽  
Vol 68 (5) ◽  
pp. 783-793 ◽  
Author(s):  
Robert P. Lattimer
Keyword(s):  
Mass Spectrometry ◽  
Thermoplastic Polyurethane ◽  
High Sensitivity ◽  
High Specificity ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Tandem Ms ◽  
Organic Additives ◽  
Soft Ionization ◽  
Modern Mass

Abstract In recent years very effective mass spectrometric methods have been developed for direct polymer compound analysis. The high sensitivity, high specificity, and superb mixture analysis capabilities of modern mass spectrometry make it an invaluable tool in the polymer industry, particularly for qualitative analysis (chemical identification) of organic additives as well as polymeric components. “Survey” mass spectra obtained with soft ionization methods — field ionization (FI-MS) and chemical ionization (CI-MS) — provide diagnostic overviews of chemical composition. The supplemental tandem (MS/MS) and atomic composition (AC-MS) techniques are used to make specific identifications of various organic ingredients. This report describes the direct mass spectrometric analysis of three different elastomer compounds. Organic additives, including curatives, were identified via thermal desorption methods in a commercial EPDM bearing as well as a diene rubber V-belt. The composition of a commercial thermoplastic polyurethane was determined via pyrolysis (Py-CI-MS). These problem-solving examples illustrate the very effective role that mass spectrometry can play in the industrial polymer analysis laboratory.

Analysis of Components in Rubber Compounds Using Mass Spectrometry

1989 ◽  
Vol 62 (3) ◽  
pp. 548-567 ◽  
Author(s):  
Robert P. Lattimer ◽  
Robert E. Harris
Keyword(s):  
Mass Spectrometry ◽  
High Sensitivity ◽  
High Specificity ◽  
Mass Spectrometric ◽  
Rubber Compound ◽  
Organic Additives ◽  
Mixture Analysis ◽  
Rubber Compounds ◽  
Invaluable Tool ◽  
Modern Mass

Abstract A large number of very successful mass-spectrometric methods have been developed for rubber-compound analysis. The high sensitivity, high specificity, and superb mixture analysis capabilities of modern mass spectrometry make it an invaluable tool in the polymer industry, particularly for qualitative identification of organic additives. In many cases, mass spectrometry can provide unique information that is not available by use of any other technique.

scholarly journals Elucidation of the Mechanism of Action for Metal Based Anticancer Drugs by Mass Spectrometry-Based Quantitative Proteomics

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 581 ◽  
Author(s):  
Shuailong Jia ◽  
Runjing Wang ◽  
Kui Wu ◽  
Hongliang Jiang ◽  
Zhifeng Du
Keyword(s):  
Mass Spectrometry ◽  
Anticancer Drugs ◽  
Mechanism Of Action ◽  
Quantitative Proteomics ◽  
High Sensitivity ◽  
Mass Spectrometric ◽  
Protein Adducts ◽  
Ionization Mass ◽  
Soft Ionization ◽  
Binding Partners

The discovery of the anticancer activity of cisplatin and its clinical application has opened a new field for studying metal-coordinated anticancer drugs. Metal-based anticancer drugs, such as cisplatin, can be transported to cells after entering into the human body and form metal–DNA or metal–protein adducts. Then, responding proteins will recognize adducts and form stable complexes. The proteins that were binding with metal-based anticancer drugs were relevant to their mechanism of action. Herein, investigation of the recognition between metal-based anticancer drugs and its binding partners will further our understanding about the pharmacology of cytotoxic anticancer drugs and help optimize the structure of anticancer drugs. The “soft” ionization mass spectrometric methods have many advantages such as high sensitivity and low sample consumption, which are suitable for the analyses of complex biological samples. Thus, MS has become a powerful tool for the identification of proteins binding or responding to metal-based anticancer drugs. In this review, we focused on the mass spectrometry-based quantitative strategy for the identification of proteins specifically responding or binding to metal-based anticancer drugs, ultimately elucidating their mechanism of action.

Direct analysis of herbicides by paper spray ionization mass spectrometry

2015 ◽  
Vol 7 (23) ◽  
pp. 9808-9816 ◽  
Author(s):  
Steven L. Reeber ◽  
Sneha Gadi ◽  
Sung-Ben Huang ◽  
Gary L. Glish
Keyword(s):  
Mass Spectrometry ◽  
Environmental Samples ◽  
Direct Analysis ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Paper Spray ◽  
Paper Spray Ionization ◽  
Rapid Mass

Paper spray ionization enables the rapid mass spectrometric analysis of environmental samples without the use of chromatography or sample cleanup techniques.

scholarly journals Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids

2010 ◽  
Vol 2010 ◽  
pp. 1-9 ◽  
Author(s):  
Yulanda M. Williamson ◽  
Hercules Moura ◽  
David Schieltz ◽  
Jon Rees ◽  
Adrian R. Woolfitt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Immune Response ◽  
Mass Spectrometric ◽  
Peptide Sequence ◽  
Spectrometric Analysis ◽  
Vaccine Preventable Disease ◽  
Label Free ◽  
Chemically Modified ◽  
Quantification Method ◽  
Major Virulence Factor

Bordetella pertussis(Bp) is the causative agent of pertussis, a vaccine preventable disease occurring primarily in children. In recent years, there has been increased reporting of pertussis. Current pertussis vaccines are acellular and consist of Bp proteins including the major virulence factor pertussis toxin (Ptx), a 5-subunit exotoxin. Variation in Ptx subunit amino acid (AA) sequence could possibly affect the immune response. A blind comparative mass spectrometric (MS) analysis of commercially available Ptx as well as the chemically modified toxoid (Ptxd) from licensed vaccines was performed to assess peptide sequence and AA coverage variability as well as relative amounts of Ptx subunits. Qualitatively, there are similarities among the various sources based on AA percent coverages and MS/MS fragmentation profiles. Additionally, based on a label-free mass spectrometry-based quantification method there is differential relative abundance of the subunits among the sources.

Extraction and Chromatography-Mass Spectrometric Analysis of the Active Principles from Selected Chinese Herbs and Other Medicinal Plants

2003 ◽  
Vol 31 (06) ◽  
pp. 927-944 ◽  
Author(s):  
Xiaosuo Wang ◽  
Vimal Kapoor ◽  
George A. Smythe
Keyword(s):  
Mass Spectrometry ◽  
Analytical Techniques ◽  
Mass Spectrometric ◽  
Chinese Herbs ◽  
Spectrometric Analysis ◽  
Toxic Substances ◽  
Herbal Extracts ◽  
Active Components ◽  
Herbal Products ◽  
Isolation And Characterization

Medicinal herbs have a long history of use in the practice of traditional Chinese medicine and a substantial body of evidence has, over recent decades, demonstrated a range of important pharmacological properties. Western biomedical researchers are examining not only the efficacy of the traditional herbal products but, through the use of a range of bioassays and analytical techniques, are developing improved methods to isolate and characterize active components. This review briefly describes the different extraction methodologies used in the preparation of herbal extracts and reviews the utility of chromatography-mass spectrometry for the analysis of their active components. In particular, applications of gas or liquid chromatography with mass spectrometry for the isolation and characterization of active components of ginseng are critically assessed. The analysis of toxic substances from herb extracts with mass spectrometric techniques is also discussed along with the potential for mass spectrometric methods to investigate the proteomics of herbal extracts.

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