scholarly journals Biological Mass Spectrometry. Mass Spectrometric Analysis of Cyanobacterial Toxins.

1996 ◽  
Vol 44 (3) ◽  
pp. 355-376 ◽  
Author(s):  
Fumio KONDO ◽  
Ken-ichi HARADA
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Biological Mass Spectrometry ◽  
Cyanobacterial Toxins

Direct analysis of herbicides by paper spray ionization mass spectrometry

2015 ◽  
Vol 7 (23) ◽  
pp. 9808-9816 ◽  
Author(s):  
Steven L. Reeber ◽  
Sneha Gadi ◽  
Sung-Ben Huang ◽  
Gary L. Glish
Keyword(s):  
Mass Spectrometry ◽  
Environmental Samples ◽  
Direct Analysis ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Paper Spray ◽  
Paper Spray Ionization ◽  
Rapid Mass

Paper spray ionization enables the rapid mass spectrometric analysis of environmental samples without the use of chromatography or sample cleanup techniques.

Selective mass spectrometric analysis of thiols using charge-tagged disulfides

The Analyst ◽  
2016 ◽  
Vol 141 (19) ◽  
pp. 5520-5526 ◽  
Author(s):  
Eric Janusson ◽  
G. Bryce McGarvey ◽  
Farhana Islam ◽  
Christine Rowan ◽  
J. Scott McIndoe
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Chemical Derivatization ◽  
Complex Matrix ◽  
Simple Chemical

A simple chemical derivatization technique was developed for electrospray ionization mass spectrometry (ESI-MS) in which thiols and disulfides may be selectively analyzed in a complex matrix and easily characterized.

The potential of mass spectrometry for the global profiling of parasite metabolomes

Parasitology ◽  
2009 ◽  
Vol 137 (9) ◽  
pp. 1409-1423 ◽  
Author(s):  
D. G. WATSON
Keyword(s):  
Mass Spectrometry ◽  
Data Processing ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Instrumental Analysis ◽  
Spectrometric Analysis ◽  
Hydrophilic Interaction ◽  
Detection And Identification ◽  
Polar Metabolites ◽  
Metabolite Detection

SUMMARYThe strengths and limitations of existing mass spectrometry methods for metabolite detection and identification are discussed. A brief review is made of the methods available for quenching and extraction of cells or organisms prior to instrumental analysis. The techniques available for carrying out mass spectrometry-based profiling of metabolomes are discussed using the analysis of extracts from trypanosomes to illustrate various points regarding methods of separation and mass spectrometric analysis. The advantages of hydrophilic interaction chromatography (HILIC) for the analysis of polar metabolites are discussed. The challenges of data processing are outlined and illustrated using the example of ThermoFisher's Sieve software. The existing literature on applications of mass spectrometry to the profiling of parasite metabolomes is reviewed.

scholarly journals Circulating Fragments of N-Terminal Pro–B-Type Natriuretic Peptides in Plasma of Heart Failure Patients

2013 ◽  
Vol 59 (10) ◽  
pp. 1523-1531 ◽  
Author(s):  
Jared Yong Yang Foo ◽  
Yunxia Wan ◽  
Benjamin L Schulz ◽  
Karam Kostner ◽  
John Atherton ◽  
...  
Keyword(s):  
Heart Failure ◽  
Mass Spectrometry ◽  
Natriuretic Peptide ◽  
Natriuretic Peptides ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Plasma Samples ◽  
Heart Failure Patients ◽  
Apparent Concentration

BACKGROUND The use of nonstandardized N-terminal pro–B-type natriuretic peptide (NT-proBNP) assays can contribute to the misdiagnosis of heart failure (HF). Moreover, there is yet to be established a common consensus regarding the circulating forms of NT-proBNP being used in current assays. We aimed to characterize and quantify the various forms of NT-proBNP in the circulation of HF patients. METHODS Plasma samples were collected from HF patients (n = 20) at rest and stored at −80 °C. NT-proBNP was enriched from HF patient plasma by use of immunoprecipitation followed by mass spectrometric analysis. Customized homogeneous sandwich AlphaLISA® immunoassays were developed and validated to quantify 6 fragments of NT-proBNP. RESULTS Mass spectrometry identified the presence of several N- and C-terminally processed forms of circulating NT-proBNP, with physiological proteolysis between Pro2-Leu3, Leu3-Gly4, Pro6-Gly7, and Pro75-Arg76. Consistent with this result, AlphaLISA immunoassays demonstrated that antibodies targeting the extreme N or C termini measured a low apparent concentration of circulating NT-proBNP. The apparent circulating NT-proBNP concentration was increased with antibodies targeting nonglycosylated and nonterminal epitopes (P < 0.05). CONCLUSIONS In plasma collected from HF patients, immunoreactive NT-proBNP was present as multiple N- and C-terminally truncated fragments of the full length NT-proBNP molecule. Immunodetection of NT-proBNP was significantly improved with the use of antibodies that did not target these terminal regions. These findings support the development of a next generation NT-proBNP assay targeting nonterminal epitopes as well as avoiding the central glycosylated region of this molecule.

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