scholarly journals Characterization of fruit trees pollen

10.5219/1096 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 634-643
Author(s):  
Matej Pospiech ◽  
Zdeňka Javůrková ◽  
Bohuslava Tremlová ◽  
Hana Běhalová

One of the options to determine botanical origin of trees or honey is the analysis of pollen grains. The characteristics of pollen grains in Czech flora has not been sufficiently described yet. Within this work, fruit trees pollen of Czech origin was characterized on the basis of morphological and spectral description of pollen grains produced by fruit species of M. domestica, P. armenica, P. persica, P. domestica, P. avium and P. cerasus. The morphological characterization results of the studied fruit species are consistent with results by other authors, but certain differences between the pollen grains of some fruit trees were confirmed. Most morphological differences were confirmed among the Malus and Prunus genera. Results of morphological and spectral analyzes further confirmed the differences between some types of fruit trees, but homogeneity remained for individual species even in mixed samples. Morphological and spectral analysis can therefore be used for botanical identification of pollen. If this knowledge is applied to pollen analysis in honey, these methods can also be used to verify the botanical origin of honey.

Author(s):  
I. Boujenane ◽  
D. Petit

SummaryThe objective of this study was a morphological characterization of five Moroccan sheep breeds (Béni Guil, Boujaâd, D'man, Sardi and Timahdite) to assess between- and within-breed variability using multivariate analyses. Fourteen morphological measurements were collected on 876 adult animals of both sexes in 98 different flocks located in 22 geographic localities of five breeds. The multiple analysis of variance revealed that significant morphological differences existed between breeds. The overall proportion of total variance due to between-breed component was 28.3 percent. The factor analysis revealed three factors accounting for 50.1, 11.8 and 7.54 percent of total variance. The first factor had high loadings for variables relating to body size, whilst the second factor had high association with traits reflecting tail length and ear size. The third factor had high loadings for wool trait. The squared Mahalanobis distance between the five sheep breeds were highly significant (P< 0.001). The largest morphological divergence was shown between Béni Guil and Sardi breeds (23.5) and the smallest one was between Boujaâd and Sardi breeds (3.54). The discriminant functions clearly discriminated and assigned 94.4 percent of Béni Guil, 79.7 of Boujaâd, 88.5 percent of D'man, 86.7 of Sardi and 80.1 percent of Timahdite sheep into their breed of origin. Overall morphological differences observed within-breeds were due for 18.1 percent to geographic locality and for 20.7 percent to flock management. It was concluded that the information reported in this study will be the basis for the establishment of characterization and selection strategies for Moroccan sheep.


Grana ◽  
2020 ◽  
pp. 1-15
Author(s):  
Priscila Fernandes De Souza ◽  
Cristina M. Ribas Dos Santos ◽  
Joseph Ree ◽  
Miguel Pedro Guerra ◽  
Rosete Pescador

Author(s):  
Dmitry D Sokoloff ◽  
Mikhail V Skaptsov ◽  
Nikolay A Vislobokov ◽  
Sergey V Smirnov ◽  
Alexander I Shmakov ◽  
...  

Abstract Finding morphological differences between cytotypes that are stable throughout their geographical range is important for understanding evolution of polyploid complexes. The ancient monocot lineage Acorus includes two groups, of which A. calamus s.l., an important medicinal plant, is a polyploid complex with a centre of diversity in Asia. European plants are sterile triploids introduced by humans. An early study suggested that plants from temperate Asia are tetraploids, but subsequent work revealed diploids and triploids rather than tetraploids in Asiatic Russia; however, cytotype diversity in Western Siberia is insufficiently known. We document the occurrence of diploids and triploids in Western Siberia. Triploids that do not differ in genome size from European Acorus are abundant in the valley of the river Ob where the ability for extensive vegetative propagation provides ecological advantages. An isolated population of aneuploid triploids with 33 chromosomes is found outside the Ob valley. Flow cytometry provides an efficient tool for identification of aneuploid plants in Acorus. All triploids are sterile, but their flowers develop uniform parthenocarpic fruits. Fruits of diploids usually vary in size within a spadix depending on the number of developing seeds. In contrast to North America, where the native diploid plants differ from the introduced triploids by the absence of a secondary midrib of the ensiform leaf blade, Siberian diploids are similar to triploids in possessing a secondary midrib. We confirm that diploids differ from triploids in the size of air lacunae in leaves, which is determined by cell number rather than cell size in septa of aerenchyma. A combination of spathe width and spadix length measured after the male stage of anthesis shows different (slightly overlapping) patterns of variation between diploids and triploids in our material.


Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1180
Author(s):  
Juan Manuel Lomillos ◽  
Marta E. Alonso

Morphometric studies in Lidia cattle are scarce due to the challenges of handling and approaching this breed of cattle. For this reason, the first morphological characterization of the Lidia breed was carried out using photogrammetry. In the present work, 264 adult individuals (184 males and 80 females), belonging to 21 different herds, were studied. A total of 20 morphological measurements and five indexes were determined in every individual. There were many positive correlations between the measures, giving the model great morphostructural harmony. Considerable internal variability of the studied parameters was observed. This breed reflected significant sexually dimorphic features and internal morphological differences between the different genetic lines of the breed. Lidia cattle are small and mostly have a sub-concave profile (58.4% males and 69.7% females). The male proportionality indexes and the relative depth of the thorax indicated great muscular development of the anterior third and high thoracic capacity. The phaneroptic information describes a mostly black animal with black mucous and hooves and an outstanding development of the dewlaps and the tail in the males.


2009 ◽  
Vol 79-82 ◽  
pp. 1675-1678
Author(s):  
Xiao Ying Lu ◽  
Xiu Hong Wang ◽  
Jian Xin Wang ◽  
Shu Xin Qu ◽  
Jie Weng

The morphological differences of chitosan (CS) in the hydroxyapatite (HA)/CS nanocomposites were investigated in detailed, which were prepared via in situ hydrothermal precipitation. The results show that the obtained nanocomposites have excellent crystallinity and the crystal has excellent ordered structure, which is important to the composites performances in the biomedical application. Moreover, the CS arrangement and crystallinity in the composites greatly depend on the hydrothermal temperature and the pH value of precipitating agent. The temperature ranging from 373 to 413K and pH value of precipitating agent ranging from 12 to 14 were favorable to the crystallization and oriented growth of CS molecules in the composites. The CS crystals with better arrangement are assembled in the order of layer-by-layer in these composites.


2017 ◽  
Vol 17 (1) ◽  
pp. 1
Author(s):  
Nikola Mićić ◽  
Gordana Đurić

Molecular confirmation of the process of differentiation of meristematic tissue of plants is primarily based on histomorphological characterization of the tissues carrying these processes. For perennial plants – fruit trees, the knowledge of these processes is important to define runtime type and pomotechnical treatments but also to identify the genes responsible for determination of the apices into a generative phase of differentiation. Which apex extraction techniques will be applied depends on the structure of buds, i.e. whether an apex differentiates only into generative elements – pure flower buds, or the differentiation goes into two directions: differentiation of leaf primordium with axillar meristematic dome and differentiation of generative elements of flowers in the peak or lateral zone of an apex – mixed buds. The fruit trees of the genus Prunus have purely flower buds and for this purpose the whole apex is taken, between protection – cover leaves, on lateral positions of all collateral buds at shoot nodes. The moment of initiation of determination and the dynamics of differentiation of generative buds are different at different type of shoots on a tree. It is therefore necessary to know the shoots growth cessation time which is in correlation with apex determination. Fruit trees of the genus Prunus are ending the growth of shoots by rejection of shoot peaks, and the cast away time depends on the type, length and the position of shoots.


Horticulturae ◽  
2021 ◽  
Vol 7 (8) ◽  
pp. 248
Author(s):  
Shiro Mori ◽  
Masaki Yahata ◽  
Ayano Kuwahara ◽  
Yurina Shirono ◽  
Yasufumi Ueno ◽  
...  

Limonium sinuatum (L.) Mill. (2n = 2x = 16) is a popular ornamental plant with dimorphism of pollen grains (type A and type B) and stigmas (papilla and cob-like). We applied polyploidy breeding to this species in order to introduce desirable traits. Tetraploid and mixoploid L. sinuatum plants were successfully obtained with oryzalin treatment of L. sinuatum ‘Early Blue’ seeds. All three tetraploids had increased leaf width, stomatal size, flower length, and pollen width compared to those of the diploid, and tetraploids had four germinal pores of pollen grains, whereas the diploid had three. All tetraploids had type A pollen grains and cob-like stigmas. Furthermore, the growth of cultivated tetraploid plants was slow, with later bolting and flowering times. Mixoploids Mixo-1 and Mixo-3 were estimated to be polyploidy periclinal chimeric plants consisting of a tetraploid L1 layer and diploid L2 layer, and Mixo-2 was estimated to be a polyploidy periclinal chimeric plant consisting of the diploid L1 layer and tetraploid L2 layer. Mixo-4 had tetraploid L1 and L2 layers. Mixoploids, except Mixo-4, had type A pollen grains and cob-like stigmas, whereas Mixo-4 had type B pollen grains and papilla stigmas. These polyploids will be useful as polyploidy breeding materials.


2017 ◽  
Vol 92 (2) ◽  
pp. 147
Author(s):  
Ariana Reis Messias Fernandes de Oliveira ◽  
Lenaldo Muniz Oliveira ◽  
José Floriano Barea Pastore ◽  
Tânia Regina dos Santos Silva ◽  
Larissa Correa do Bonfim Costa

The genus Lippia L. is represented by several aromatic species, producing essential oils with biological activity, especially antimicrobial.  The semiarid region of Bahia contains several Lippia species that could be economically exploited. This study aimed to characterize morphological and agronomic traits of four species of Lippia native of the semiarid region. The species were collected in a number of cities of the state of Bahia. The experimental design was performed in randomized blocks with four repetitions and four plants per repetition for each species. We conducted quantitative and qualitative morphological characterizations, and evaluated the characters of stem, leaf and inflorescence; in addition to the agronomic characterization, with the fresh and dry matter of inflorescences, we evaluated leaves and stem and essential oil content and yield. The results showed morphological differences between the species for all traits evaluated, except for the number of flowers per inflorescence. The agronomical characterization showed differences for all measured characters, and the species Lippia lasiocalycina was distinguished by the essential oil yield.


Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2094
Author(s):  
Jose Manuel Ageitos ◽  
Sandra Robla ◽  
Lorena Valverde-Fraga ◽  
Marcos Garcia-Fuentes ◽  
Noemi Csaba

Pollen grains are natural microcapsules comprised of the biopolymer sporopollenin. The uniformity and special tridimensional architecture of these sporopollenin structures confer them attractive properties such as high resistance and improved bioadhesion. However, natural pollen can be a source of allergens, hindering its biomedical applicability. Several methods have been developed to remove internal components and allergenic compounds, usually involving long and laborious processes, which often cannot be extended to other pollen types. In this work, we propose an abridged protocol to produce stable and pristine hollow pollen microcapsules, together with a complete physicochemical and morphological characterization of the intermediate and final products. The optimized procedure has been validated for different pollen samples, also producing sporopollenin microcapsules from Matricaria species for the first time. Pollen microcapsules obtained through this protocol presented low protein content (4.4%), preserved ornamented morphology with a nanoporous surface, and low product density (0.14 g/cm3). These features make them interesting candidates from a pharmaceutical perspective due to the versatility of this biomaterial as a drug delivery platform.


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