scholarly journals Co–Culture with Amniotic Membrane Stem Cells for Oocyte Maturation and Early Embryonic Development in Bovine

2015 ◽  
Vol 60 (2) ◽  
pp. 393-398
Author(s):  
Kil–Woo Han ◽  
Ju Lan Chun ◽  
Ji Hey Lee ◽  
Keun–Jung Kim ◽  
Kyung-Bon Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Development ◽  
Oocyte Maturation ◽  
Amniotic Membrane ◽  
Early Embryonic Development

scholarly journals BAF Complex in Embryonic Stem Cells and Early Embryonic Development

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Heyao Zhang ◽  
Xuepeng Wang ◽  
Jingsheng Li ◽  
Ronghua Shi ◽  
Ying Ye
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Development ◽  
Embryonic Stem ◽  
Early Embryonic Development ◽  
Mouse Escs ◽  
Baf Complex ◽  
Pluripotency Gene ◽  
Chromatin Remodeling Complexes ◽  
Gene Regulatory

Embryonic stem cells (ESCs) can self-renew indefinitely and maintain their pluripotency status. The pluripotency gene regulatory network is critical in controlling these properties and particularly chromatin remodeling complexes. In this review, we summarize the research progresses of the functional and mechanistic studies of BAF complex in mouse ESCs and early embryonic development. A discussion of the mechanistic bases underlying the distinct phenotypes upon the deletion of different BAF subunits in ESCs and embryos will be highlighted.

Expression of microRNAs and RNA-Binding Protein DND1 During Oocyte Maturation and Early Embryonic Development in the Pig.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 323-323
Author(s):  
Cai-Xia Yang ◽  
Elane C. Wright ◽  
Robyn Scanlon ◽  
Ben Selman ◽  
Randall S. Prather ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Early Embryonic Development

scholarly journals Enucleolation and nucleolus transfer in mammalian oocytes and zygotes

2019 ◽  
Vol 63 (3-4-5) ◽  
pp. 253-258
Author(s):  
Michal Benc ◽  
Josef Jr. Fulka ◽  
František Strejček ◽  
Martin Morovič ◽  
Matej Murín ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Real Function ◽  
Morphological Structure ◽  
Germinal Vesicle ◽  
Early Embryonic Development ◽  
The Real ◽  
Germinal Vesicles

The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygot PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easily manipulated, i.e. removed from GVs/PNs or eventually transferred into another oocyte/zygote. Thus, with the help of micromanipulation techniques it was possible to uncover the real function(s) they play in processes of oocyte maturation and early embryonic development. The purpose of our review is to describe briefly the micromanipulation techniques that can be used for oocyte/zygote nucleoli manipulation. Moreover, we present some examples of results that were obtained in nucleolus manipulation experiments.

Embryo co-culture with bovine amniotic membrane stem cells can enhance the cryo-survival of IVF-derived bovine blastocysts comparable with co-culture with bovine oviduct epithelial cells

Zygote ◽  
2020 ◽  
pp. 1-6
Author(s):  
Shayan Nejat-Dehkordi ◽  
Ebrahim Ahmadi ◽  
Abolfazl Shirazi ◽  
Hassan Nazari ◽  
Naser Shams-Esfandabadi
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Epithelial Cells ◽  
Embryonic Development ◽  
Amniotic Membrane ◽  
Biological Activities ◽  
Survival Rates ◽  
Blastocyst Stage ◽  
Oviduct Epithelial Cells

Summary Culture conditions have a profound effect on the quality of in vitro-produced embryos. Co-culturing embryos with somatic cells has some beneficial effects on embryonic development. Considering the ability of stem cells to secrete a broad range of growth factors with different biological activities, we hypothesized that bovine amniotic membrane stem cells (bAMSCs) might be superior to bovine oviduct epithelial cells (BOECs) in supporting embryonic development and enhancing their cryo-survival. Bovine abattoir-derived oocytes were matured and fertilized in vitro. The resultant presumptive zygotes were then cultured up to the blastocyst stage in the following groups: (i) co-culture with bAMSCs, (ii) co-culture with BOECs, and (iii) cell-free culture (Con). Embryos that reached the blastocyst stage were vitrified and warmed, and their post-warming re-expansion, survival and hatching rates were evaluated after 72 h culture. Results showed that the cleavage, blastocyst, and 2 h post-warming re-expansion rates of embryos did not differ between groups. However, their survival rates in BOEC and bAMSC groups were significantly higher compared with the control (72.7, 75.6 and 37.5%, respectively, P < 0.05). In conclusion, our results showed that the cryo-survivability of IVF-derived bovine embryos could be improved through co-culturing with bAMSCs. Moreover, considering the possibility to provide multiple passages from bAMSCs compared with BOECs, due to their stemness properties and their ability to produce growth factors, the use of bAMSCs is a good alternative to BOECs in embryo co-culture systems.

Effect of PRL on In Vitro Follicle Growth, In Vitro Oocyte Maturation, Fertilization and Early Embryonic Development in Mice

2009 ◽  
Vol 11 (2) ◽  
pp. 293-300 ◽  
Author(s):  
Erasmia Kiapekou ◽  
Dimitris Loutradis ◽  
George Mastorakos ◽  
Ritsa Bletsa ◽  
Panagiotis Beretsos ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Early Embryonic Development ◽  
Follicle Growth ◽  
In Vitro Oocyte Maturation

Analysis of mRNA associated factors during bovine oocyte maturation and early embryonic development

2009 ◽  
Vol 76 (12) ◽  
pp. 1208-1219 ◽  
Author(s):  
Corinna Siemer ◽  
Tatjana Smiljakovic ◽  
Monika Bhojwani ◽  
Claus Leiding ◽  
Wilhelm Kanitz ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Associated Factors ◽  
Early Embryonic Development ◽  
Bovine Oocyte

Iloprost, a prostacyclin analogue, stimulates meiotic maturation and early embryonic development in pigs

2010 ◽  
Vol 22 (2) ◽  
pp. 437 ◽  
Author(s):  
Ji-Su Kim ◽  
Jung-Il Chae ◽  
Bong-Seok Song ◽  
Kyu-Sun Lee ◽  
Young-Kug Choo ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Embryonic Development ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Structural Integrity ◽  
Early Embryonic Development ◽  
Mitogen Activated Protein Kinase ◽  
Meiotic Maturation ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Intracellular Camp

Oviduct fluid contains various cytokines and growth factors that enhance the embryo development during the preimplantation period. In hatched embryos, prostacyclin (PGI2) improves implantation, but its role during oocyte maturation and early embryo development remains contentious. Therefore, in the present study, we examined the effects of a PGI2 analogue (iloprost) on meiotic maturation and early embryonic development in pigs, as well on the structural integrity, mitochondrial membrane potential and apoptosis in blastocysts. First, meiotic maturation in pig oocytes was examined in the presence of increasing concentrations of iloprost (1, 5 and 10 μM). After IVM, a higher proportion of iloprost-treated compared with untreated oocytes was in MII (90.0% v. 65.7%, respectively; P < 0.05). In addition, protein kinase A activity increased in iloprost-treated oocytes, indicating increased intracellular cAMP concentrations. After 22 h iloprost treatment (44 h total incubation time), western blotting demonstrated increased expression of extracellular signal-regulated kinase (ERK) 1/2, phosphorylated (p-) ERK1/2, cAMP response element-binding protein (CREB), p-CREB and cyclo-oxygenase-2, indicating activation of the mitogen-activated protein kinase and PGI2 pathways. In addition, the frequency of polyspermy decreased in iloprost-treated oocytes (19.9%) compared with control (35.8%), whereas the rate of blastocyst formation increased (P < 0.05). Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) showed that the number of nuclei containing fragmented DNA at the blastocyst stage decreased in the iloprost-treated group compared with control (1.2% v. 3.6%, respectively). In conclusion, iloprost appears to play a direct role in porcine oocyte maturation by enhancing blastocyst structure and survival.

scholarly journals Morphological features of lipid droplet transition during porcine oocyte fertilisation and early embryonic development to blastocyst in vivo and in vitro

Zygote ◽  
2002 ◽  
Vol 10 (4) ◽  
pp. 355-366 ◽  
Author(s):  
Kazuhiro Kikuchi ◽  
Hans Ekwall ◽  
Paisan Tienthai ◽  
Yasuhiro Kawai ◽  
Junko Noguchi ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Lipid Droplets ◽  
Early Embryonic Development ◽  
Oocyte Activation ◽  
Energy Status ◽  
Thin Sections ◽  
Porcine Oocyte

Lipid content in mammalian oocytes or embryos differs among species, with bovine and porcine oocytes and embryos showing large cytoplasmic droplets. These droplets are considered to play important roles in energy metabolism during oocyte maturation, fertilisation and early embryonic development, and also in the freezing ability of oocytes or embryos; however, their detailed distribution or function is not well understood. In the present study, changes in the distribution and morphology of porcine lipid droplets during in vivo and in vitro fertilisation, in contrast to parthenogenetic oocyte activation, as well as during their development to blastocyst stage, were evaluated by transmission electron microscopy (TEM). The analysis of semi-thin and ultra-thin sections by TEM showed conspicuous, large, electron-dense lipid droplets, sometimes associated with mitochondrial aggregates in the oocytes, irrespective of whether the oocytes had been matured in vivo or in vitro. Immediately after sperm penetration, the electron density of the lipid droplets was lost in both the in vivo and in vitro oocytes, the reduction being most evident in the oocytes developed in vitro. Density was restored in the pronculear oocytes, fully in the in vivo specimens but only partially in the in vitro ones. The number and size of the droplets seemed, however, to have decreased. At 2- to 4-cell and blastocyst stages, the features of the lipid droplets were almost the same as those of pronuclear oocytes, showing a homogeneous or saturated density in the in vivo embryos but a marbled or partially saturated appearance in the in vitro embryos. In vitro matured oocytes undergoing parthenogenesis had lipid droplets that resembled those of fertilised oocytes until the pronuclear stage. Overall, results indicate variations in both the morphology and amount of cytoplasmic lipid droplets during porcine oocyte maturation, fertilisation and early embryo development as well as differences between in vivo and in vitro development, suggesting both different energy status during preimplantation development in pigs and substantial differences between in vitro and in vivo development.

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