Analysis of mRNA associated factors during bovine oocyte maturation and early embryonic development

2009 ◽  
Vol 76 (12) ◽  
pp. 1208-1219 ◽  
Author(s):  
Corinna Siemer ◽  
Tatjana Smiljakovic ◽  
Monika Bhojwani ◽  
Claus Leiding ◽  
Wilhelm Kanitz ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Associated Factors ◽  
Early Embryonic Development ◽  
Bovine Oocyte

scholarly journals The effect of valproic acid on bovine oocyte maturation and early embryonic development in vitro

2013 ◽  
Vol 66 (3) ◽  
pp. 525-532 ◽  
Author(s):  
Haixia Gao ◽  
Haidong Bai ◽  
Xudong Ao ◽  
Rula Sa ◽  
Huimin Wang ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Embryonic Development ◽  
Oocyte Maturation ◽  
Early Embryonic Development ◽  
Bovine Oocyte ◽  
Development In Vitro

scholarly journals Requirement of the transcription factor USF1 in bovine oocyte and early embryonic development

Reproduction ◽  
2015 ◽  
Vol 149 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Tirtha K Datta ◽  
Sandeep K Rajput ◽  
Gabbine Wee ◽  
KyungBon Lee ◽  
Joseph K Folger ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Embryonic Development ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Developmental Competence ◽  
Early Embryonic Development ◽  
Bovine Oocyte ◽  
Cell Stage ◽  
Oocyte Competence ◽  
E Box

Upstream stimulating factor 1 (USF1) is a basic helix–loop–helix transcription factor that specifically binds to E-box DNA motifs, knowncis-elements of key oocyte expressed genes essential for oocyte and early embryonic development. However, the functional and regulatory role of USF1 in bovine oocyte and embryo development is not understood. In this study, we demonstrated thatUSF1mRNA is maternal in origin and expressed in a stage specific manner during the course of oocyte maturation and preimplantation embryonic development. Immunocytochemical analysis showed detectable USF1 protein during oocyte maturation and early embryonic development with increased abundance at 8–16-cell stage of embryo development, suggesting a potential role in embryonic genome activation. Knockdown ofUSF1in germinal vesicle stage oocytes did not affect meiotic maturation or cumulus expansion, but caused significant changes in mRNA abundance for genes associated with oocyte developmental competence. Furthermore, siRNA-mediated depletion ofUSF1in presumptive zygote stage embryos demonstrated thatUSF1is required for early embryonic development to the blastocyst stage. A similar (USF2) yet unique (TWIST2) expression pattern during oocyte and early embryonic development for related E-box binding transcription factors known to cooperatively bind USF1 implies a potential link to USF1 action. This study demonstrates that USF1 is a maternally derived transcription factor required for bovine early embryonic development, which also functions in regulation ofJY1, GDF9, andFSTgenes associated with oocyte competence.

Expression of microRNAs and RNA-Binding Protein DND1 During Oocyte Maturation and Early Embryonic Development in the Pig.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 323-323
Author(s):  
Cai-Xia Yang ◽  
Elane C. Wright ◽  
Robyn Scanlon ◽  
Ben Selman ◽  
Randall S. Prather ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Early Embryonic Development

scholarly journals Enucleolation and nucleolus transfer in mammalian oocytes and zygotes

2019 ◽  
Vol 63 (3-4-5) ◽  
pp. 253-258
Author(s):  
Michal Benc ◽  
Josef Jr. Fulka ◽  
František Strejček ◽  
Martin Morovič ◽  
Matej Murín ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Real Function ◽  
Morphological Structure ◽  
Germinal Vesicle ◽  
Early Embryonic Development ◽  
The Real ◽  
Germinal Vesicles

The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygot PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easily manipulated, i.e. removed from GVs/PNs or eventually transferred into another oocyte/zygote. Thus, with the help of micromanipulation techniques it was possible to uncover the real function(s) they play in processes of oocyte maturation and early embryonic development. The purpose of our review is to describe briefly the micromanipulation techniques that can be used for oocyte/zygote nucleoli manipulation. Moreover, we present some examples of results that were obtained in nucleolus manipulation experiments.

Effect of PRL on In Vitro Follicle Growth, In Vitro Oocyte Maturation, Fertilization and Early Embryonic Development in Mice

2009 ◽  
Vol 11 (2) ◽  
pp. 293-300 ◽  
Author(s):  
Erasmia Kiapekou ◽  
Dimitris Loutradis ◽  
George Mastorakos ◽  
Ritsa Bletsa ◽  
Panagiotis Beretsos ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Early Embryonic Development ◽  
Follicle Growth ◽  
In Vitro Oocyte Maturation

Iloprost, a prostacyclin analogue, stimulates meiotic maturation and early embryonic development in pigs

2010 ◽  
Vol 22 (2) ◽  
pp. 437 ◽  
Author(s):  
Ji-Su Kim ◽  
Jung-Il Chae ◽  
Bong-Seok Song ◽  
Kyu-Sun Lee ◽  
Young-Kug Choo ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Embryonic Development ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Structural Integrity ◽  
Early Embryonic Development ◽  
Mitogen Activated Protein Kinase ◽  
Meiotic Maturation ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Intracellular Camp

Oviduct fluid contains various cytokines and growth factors that enhance the embryo development during the preimplantation period. In hatched embryos, prostacyclin (PGI2) improves implantation, but its role during oocyte maturation and early embryo development remains contentious. Therefore, in the present study, we examined the effects of a PGI2 analogue (iloprost) on meiotic maturation and early embryonic development in pigs, as well on the structural integrity, mitochondrial membrane potential and apoptosis in blastocysts. First, meiotic maturation in pig oocytes was examined in the presence of increasing concentrations of iloprost (1, 5 and 10 μM). After IVM, a higher proportion of iloprost-treated compared with untreated oocytes was in MII (90.0% v. 65.7%, respectively; P < 0.05). In addition, protein kinase A activity increased in iloprost-treated oocytes, indicating increased intracellular cAMP concentrations. After 22 h iloprost treatment (44 h total incubation time), western blotting demonstrated increased expression of extracellular signal-regulated kinase (ERK) 1/2, phosphorylated (p-) ERK1/2, cAMP response element-binding protein (CREB), p-CREB and cyclo-oxygenase-2, indicating activation of the mitogen-activated protein kinase and PGI2 pathways. In addition, the frequency of polyspermy decreased in iloprost-treated oocytes (19.9%) compared with control (35.8%), whereas the rate of blastocyst formation increased (P < 0.05). Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) showed that the number of nuclei containing fragmented DNA at the blastocyst stage decreased in the iloprost-treated group compared with control (1.2% v. 3.6%, respectively). In conclusion, iloprost appears to play a direct role in porcine oocyte maturation by enhancing blastocyst structure and survival.

scholarly journals Morphological features of lipid droplet transition during porcine oocyte fertilisation and early embryonic development to blastocyst in vivo and in vitro

Zygote ◽  
2002 ◽  
Vol 10 (4) ◽  
pp. 355-366 ◽  
Author(s):  
Kazuhiro Kikuchi ◽  
Hans Ekwall ◽  
Paisan Tienthai ◽  
Yasuhiro Kawai ◽  
Junko Noguchi ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Lipid Droplets ◽  
Early Embryonic Development ◽  
Oocyte Activation ◽  
Energy Status ◽  
Thin Sections ◽  
Porcine Oocyte

Lipid content in mammalian oocytes or embryos differs among species, with bovine and porcine oocytes and embryos showing large cytoplasmic droplets. These droplets are considered to play important roles in energy metabolism during oocyte maturation, fertilisation and early embryonic development, and also in the freezing ability of oocytes or embryos; however, their detailed distribution or function is not well understood. In the present study, changes in the distribution and morphology of porcine lipid droplets during in vivo and in vitro fertilisation, in contrast to parthenogenetic oocyte activation, as well as during their development to blastocyst stage, were evaluated by transmission electron microscopy (TEM). The analysis of semi-thin and ultra-thin sections by TEM showed conspicuous, large, electron-dense lipid droplets, sometimes associated with mitochondrial aggregates in the oocytes, irrespective of whether the oocytes had been matured in vivo or in vitro. Immediately after sperm penetration, the electron density of the lipid droplets was lost in both the in vivo and in vitro oocytes, the reduction being most evident in the oocytes developed in vitro. Density was restored in the pronculear oocytes, fully in the in vivo specimens but only partially in the in vitro ones. The number and size of the droplets seemed, however, to have decreased. At 2- to 4-cell and blastocyst stages, the features of the lipid droplets were almost the same as those of pronuclear oocytes, showing a homogeneous or saturated density in the in vivo embryos but a marbled or partially saturated appearance in the in vitro embryos. In vitro matured oocytes undergoing parthenogenesis had lipid droplets that resembled those of fertilised oocytes until the pronuclear stage. Overall, results indicate variations in both the morphology and amount of cytoplasmic lipid droplets during porcine oocyte maturation, fertilisation and early embryo development as well as differences between in vivo and in vitro development, suggesting both different energy status during preimplantation development in pigs and substantial differences between in vitro and in vivo development.

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