scholarly journals EXPRESSION OF SELECTED CANDIDATE GENES DURING IN VITRO OOCYTE MATURATION AND EARLY EMBRYONIC DEVELOPMENT OF IN VITRO GENERATED EGYPTIAN BUFFALO (BUBALUS BUBALIS) EMBRYOS

2012 ◽  
Vol 49 (1) ◽  
pp. 19-28 ◽  
Keyword(s):  
Embryonic Development ◽  
Candidate Genes ◽  
Oocyte Maturation ◽  
Bubalus Bubalis ◽  
Early Embryonic Development ◽  
In Vitro Oocyte Maturation ◽  
Egyptian Buffalo

Effect of PRL on In Vitro Follicle Growth, In Vitro Oocyte Maturation, Fertilization and Early Embryonic Development in Mice

2009 ◽  
Vol 11 (2) ◽  
pp. 293-300 ◽  
Author(s):  
Erasmia Kiapekou ◽  
Dimitris Loutradis ◽  
George Mastorakos ◽  
Ritsa Bletsa ◽  
Panagiotis Beretsos ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Early Embryonic Development ◽  
Follicle Growth ◽  
In Vitro Oocyte Maturation

scholarly journals Morphological features of lipid droplet transition during porcine oocyte fertilisation and early embryonic development to blastocyst in vivo and in vitro

Zygote ◽  
2002 ◽  
Vol 10 (4) ◽  
pp. 355-366 ◽  
Author(s):  
Kazuhiro Kikuchi ◽  
Hans Ekwall ◽  
Paisan Tienthai ◽  
Yasuhiro Kawai ◽  
Junko Noguchi ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Lipid Droplets ◽  
Early Embryonic Development ◽  
Oocyte Activation ◽  
Energy Status ◽  
Thin Sections ◽  
Porcine Oocyte

Lipid content in mammalian oocytes or embryos differs among species, with bovine and porcine oocytes and embryos showing large cytoplasmic droplets. These droplets are considered to play important roles in energy metabolism during oocyte maturation, fertilisation and early embryonic development, and also in the freezing ability of oocytes or embryos; however, their detailed distribution or function is not well understood. In the present study, changes in the distribution and morphology of porcine lipid droplets during in vivo and in vitro fertilisation, in contrast to parthenogenetic oocyte activation, as well as during their development to blastocyst stage, were evaluated by transmission electron microscopy (TEM). The analysis of semi-thin and ultra-thin sections by TEM showed conspicuous, large, electron-dense lipid droplets, sometimes associated with mitochondrial aggregates in the oocytes, irrespective of whether the oocytes had been matured in vivo or in vitro. Immediately after sperm penetration, the electron density of the lipid droplets was lost in both the in vivo and in vitro oocytes, the reduction being most evident in the oocytes developed in vitro. Density was restored in the pronculear oocytes, fully in the in vivo specimens but only partially in the in vitro ones. The number and size of the droplets seemed, however, to have decreased. At 2- to 4-cell and blastocyst stages, the features of the lipid droplets were almost the same as those of pronuclear oocytes, showing a homogeneous or saturated density in the in vivo embryos but a marbled or partially saturated appearance in the in vitro embryos. In vitro matured oocytes undergoing parthenogenesis had lipid droplets that resembled those of fertilised oocytes until the pronuclear stage. Overall, results indicate variations in both the morphology and amount of cytoplasmic lipid droplets during porcine oocyte maturation, fertilisation and early embryo development as well as differences between in vivo and in vitro development, suggesting both different energy status during preimplantation development in pigs and substantial differences between in vitro and in vivo development.

Tretinoin-loaded lipid-core nanocapsules decrease reactive oxygen species levels and improve bovine embryonic development during in vitro oocyte maturation

2015 ◽  
Vol 58 ◽  
pp. 131-139 ◽  
Author(s):  
Caroline Gomes Lucas ◽  
Mariana Härter Remião ◽  
Eliza Rossi Komninou ◽  
William Borges Domingues ◽  
Cristina Haas ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Embryonic Development ◽  
Oocyte Maturation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Lipid Core ◽  
In Vitro Oocyte Maturation

scholarly journals The effect of valproic acid on bovine oocyte maturation and early embryonic development in vitro

2013 ◽  
Vol 66 (3) ◽  
pp. 525-532 ◽  
Author(s):  
Haixia Gao ◽  
Haidong Bai ◽  
Xudong Ao ◽  
Rula Sa ◽  
Huimin Wang ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Embryonic Development ◽  
Oocyte Maturation ◽  
Early Embryonic Development ◽  
Bovine Oocyte ◽  
Development In Vitro

scholarly journals Coenzyme Q10 Supplementation Effects on In Vitro Maturation, Fertilization, and Early Embryonic Development in Pigs

2019 ◽  
Vol 119 (2) ◽  
pp. 2
Author(s):  
Caitlin Streacker ◽  
Brian D. Whitaker
Keyword(s):  
Embryonic Development ◽  
Oocyte Maturation ◽  
Coenzyme Q10 ◽  
Early Embryonic Development ◽  
Mitochondrial Activity ◽  
Mitochondrial Membranes ◽  
Vitro Fertilization ◽  
Treatment Groups ◽  
Development Rates

The objective of this study was to determine the reduction of polyspermic penetration, and increase of mitochondrial activity, in early pig embryonic development by supplementing different concentrations of coenzyme Q10 during oocyte maturation. Oocytes (n = 1,100) were supplemented during the last 24 h of maturation with 0 (control), 10, 50, or 100 μM of coenzyme Q10. After in vitro fertilization (IVF), embryos were evaluated for fertilization kinetics (penetration, polyspermic penetration, male pronuclear formation), and subsequent embryonic development and mitochondrial activity. Supplementation of 100 μM coenzyme Q10 was detrimental to the oocytes, as they had significantly lower (p < 0.05) fertilization kinetic and early embryonic development rates to the other treatment groups. There were no differences in fertilization kinetic and early embryonic development rates between the 0, 10 and 50 μM coenzyme Q10 treatment groups. Oocytes, matured in medium supplemented with 50 μM coenzyme Q10, ultimately developed into embryos with a significantly greater (p < 0.05) presence of intact mitochondrial membranes (observed at both 48 and 144 h post-IVF) compared to oocytes not supplemented with coenzyme Q10. In summary, supplementation of 100 μM coenzyme Q10 during oocyte maturation is detrimental, yet supplementation of 50 μM coenzyme Q10 leads to a higher occurrence of intact mitochondrial membranes in the in vitro produced pig embryos.

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