scholarly journals Quantification of cytotoxic levels of exotoxin A ofPseudomonas aeruginosa cultivated on simple synthetic medium

2019 ◽  
Author(s):  
Ibtesam Ghadban Auda ◽  
Afrah Fahad Abdulkareem
Keyword(s):  
Synthetic Medium ◽  
Exotoxin A ◽  
Simple Synthetic Medium

A medium for commercial production of the halophilic Micrococcus nuclease

1979 ◽  
Vol 25 (9) ◽  
pp. 1113-1116 ◽  
Author(s):  
Masahiro Kamekura ◽  
Hiroshi Onishi
Keyword(s):  
Ammonium Sulfate ◽  
Synthetic Medium ◽  
Commercial Production ◽  
Good Growth ◽  
Moderate Halophile ◽  
Sucrose Medium ◽  
Simple Medium ◽  
Simple Synthetic Medium

A simple synthetic medium (glutamate–sucrose medium) was devised for production, during growth in shaken flasks, of extracellular halophilic nuclease (nuclease H) by a moderate halophile, Micrococcus varians subsp. halophilus. A simple medium consisting of 0.7% ammonium sulfate, 1.0% glucose, minerals, three vitamins, and 2 M NaCl gave good growth and excellent production of nuclease H in a jar fermentor when the pH was adjusted to 7.5 to 8.0 during cultivation.

scholarly journals An improved assay method for neutralizing antibodies against influenza viruses

1958 ◽  
Vol 56 (3) ◽  
pp. 415-426 ◽  
Author(s):  
S. Fazekas de St Groth ◽  
J. Withell ◽  
K. J. Lafferty
Keyword(s):  
Influenza Virus ◽  
Neutralizing Antibodies ◽  
Synthetic Medium ◽  
Host Tissue ◽  
Influenza Viruses ◽  
Assay Method ◽  
Techniques Of Neutralization ◽  
Set Up ◽  
Simple Synthetic Medium ◽  
Plastic Trays

1. A method has been developed for the assay of antisera against influenza viruses.2. Surviving bits of allantois-on-shell serve as host tissue, eighty to one hundred units being provided by a single egg. The test is set up in plastic trays, and uses a simple synthetic medium.3. The method has worked equally well with the ten representative strains of influenza virus tested. It surpasses the orthodox techniques of neutralization in mice or whole eggs as regards sensitivity, accuracy and economy.

NITROGEN AND VITAMIN REQUIREMENTS OF PSEUDOMONAS HYDROPHILA

1953 ◽  
Vol 31 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Florence R. Tamboline
Keyword(s):  
Yeast Extract ◽  
Synthetic Medium ◽  
Pantothenic Acid ◽  
Ammonium Phosphate ◽  
Ammonium Salts ◽  
Ammonium Citrate ◽  
Mineral Salts ◽  
Vitamin Requirements ◽  
Casamino Acids ◽  
Simple Synthetic Medium

Yeast extract and vitamin-free casamino acids were found to be equivalent as sources of nitrogen for the growth of Pseudomonas hydrophila in a glucose – mineral salts medium. The addition of a mixture of thiamine, riboflavin, pantothenic acid, pyridoxine, nicotinic acid, para-aminobenzoic acid, biotin, and folic acid to the medium containing vitamin-free casamino acids did not stimulate growth. About 67% as much growth was obtained with a mixture of 20 amino acids and asparagine as with the vitamin-free casamino acids and the mixture could be replaced by any one of asparagine, aspartic acid, serine, and alanine. Of the 11 simple nitrogen compounds tested, including urea, nitrates, and ammonium salts, only ammonium citrate and dibasic ammonium phosphate were utilized appreciably. A simple synthetic medium consisting of ammonium citrate, glucose, and mineral salts was found to give approximately the same amount of growth as the more complex yeast extract – glucose – mineral salts medium.

EFFECT OF FERROCYANIDE ON GROWTH AND ACID PRODUCTION OF ASPERGILLUS NIGER

1955 ◽  
Vol 1 (8) ◽  
pp. 644-652 ◽  
Author(s):  
S. M. Martin
Keyword(s):  
Citric Acid ◽  
Trace Metals ◽  
Aspergillus Niger ◽  
Acid Production ◽  
Zinc Concentration ◽  
Synthetic Medium ◽  
Inhibition Of Growth ◽  
Iron And Zinc ◽  
Simple Synthetic Medium

Growth of Aspergillus niger, in a simple synthetic medium, is inhibited by ferrocyanide ion in concentrations below 1 p.p.m. This inhibition can be reversed by increasing the iron and zinc concentration of the medium. Acid production, however, is inhibited only 50% by 200 p.p.m. ferrocyanide and this inhibition is not reversible by trace metals. Neither the inhibition of growth nor that of acid production is the result of release of cyanide from ferrocyanide. It is suggested that ferrocyanide has at least two functions in the treatment of substrates for use in the production of citric acid. The first is to remove deleterious trace metals from the medium whereas the second is to act as a direct inhibitor of growth of the mold.

A SIMPLE SYNTHETIC MEDIUM FOR THE CULTIVATION OF BACTERIA

Science ◽  
1926 ◽  
Vol 64 (1654) ◽  
pp. 254-254 ◽  
Author(s):  
A. F. Dolloff
Keyword(s):  
Synthetic Medium ◽  
Simple Synthetic Medium

Rational development of a simple synthetic medium for the sustained growth ofLactococcus lactis

1995 ◽  
Vol 79 (1) ◽  
pp. 108-116 ◽  
Author(s):  
M. Cocaign-Bousquet ◽  
C. Garrigues ◽  
L. Novak ◽  
N.D. Lindley ◽  
P. Loublere
Keyword(s):  
Synthetic Medium ◽  
Sustained Growth ◽  
Rational Development ◽  
Simple Synthetic Medium
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