scholarly journals Growth kinetics and characterization of human dental pulp stem cells: Comparison between third molar and first premolar teeth

2017 ◽  
pp. 0-0 ◽  
Author(s):  
D Mehrabani ◽  
P Mahdiyar ◽  
K Torabi ◽  
R Robati ◽  
S Zare ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Kinetics ◽  
Dental Pulp ◽  
Third Molar ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

Preparation and characterization of titanium—segmented polyurethane composites for bone tissue engineering

2018 ◽  
Vol 33 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Fernando Javier Aguilar-Perez ◽  
Rossana Vargas-Coronado ◽  
Jose Manuel Cervantes-Uc ◽  
Juan Valerio Cauich-Rodriguez ◽  
Raul Rosales-Ibañez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Soft Segment ◽  
Physicochemical Characterization ◽  
Dental Pulp Stem Cells ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Human Dental Pulp ◽  
Polyurethane Composites

Segmented polyurethanes were prepared with polycaprolactone diol as soft segment and 4,4-methylene-bis cyclohexyl diisocyanate and l-glutamine as the rigid segment. These polyurethanes were filled with 1 wt.% to 5 wt.% titanium particles (Ti), physicochemically characterized and their biocompatibility assessed using human dental pulp stem cells and mice osteoblasts. Physicochemical characterization showed that composites retained the properties of the semicrystalline polyurethane as they exhibited a glass transition temperature (Tg) between −35°C and −45°C, melting temperature (Tm) at 52°C and crystallinity close to 40% as determined by differential scanning calorimetry. In agreement with this, X-ray diffraction showed reflections at 21.3° and 23.6° for polycaprolactone diol and reflections at 35.1°, 38.4°, and 40.2° for Ti particles suggesting that these particles are not acting as nucleating sites. The addition of up to 5 wt.% of Ti reduced both, tensile strength and maximum strain from 1.9 MPa to 1.2 MPa, and from 670% to 172% for pristine and filled polyurethane, respectively. Although there were differences between composites at low strain rates, no significant differences in mechanical behavior were observed at higher strain rate where a tensile stress of 8.5 MPa and strain of 223% were observed for 5 wt.% composites. The addition to titanium particles had a beneficial effect on both human dental pulp stem cells and osteoblasts viability, as it increased with the amount of titanium in composites up to 10 days of incubation.

Characterization of p75 neurotrophin receptor expression in human dental pulp stem cells

2016 ◽  
Vol 53 (1) ◽  
pp. 90-98 ◽  
Author(s):  
Wenru Pan ◽  
Karlea L. Kremer ◽  
Xenia Kaidonis ◽  
Victoria E. Ludlow ◽  
Mary‐Louise Rogers ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Receptor Expression ◽  
Neurotrophin Receptor ◽  
P75 Neurotrophin Receptor ◽  
Human Dental Pulp

Analysis of growth kinetics and colony-forming unit efficiency of human dental pulp stem cells cultured in different media and supplements

2021 ◽  
Vol 16 (7) ◽  
pp. 203-210
Author(s):  
Kumar Chethan ◽  
Shishir Shetty ◽  
Basan Gowda Kurkalli ◽  
Veena Shetty ◽  
Kumar Basavarajappa Mohana
Keyword(s):  
Stem Cells ◽  
Growth Kinetics ◽  
Dental Pulp ◽  
Culture Conditions ◽  
Dental Pulp Stem Cells ◽  
Population Doubling ◽  
Animal Origin ◽  
Dental Tissues ◽  
Colony Forming Unit ◽  
Human Dental Pulp

Dental tissues are considered as ideal autologous sources of multipotent stem cells. Presently, human dental pulp stem cells (DPSCs) are largely being isolated and expanded in media containing fetal bovine serum (FBS). However, the use of FBS has limitations due to its animal origin. Therefore, the present study evaluated the morphology, proliferation rate, population doubling time (PDT) and colony-forming unit fibroblast (CFU-F) efficiency of DPSCs cultured in animal serum-containing medium (SCM) and serumfree medium (SFM) in addition to serum-free culture conditions by supplementing human blood-derivatives such as platelet lysate (PL), fresh frozen plasma (FFP) and umbilical cord blood serum (UCS) at 2.5%, 5% and 7.5% concentrations. Established DPSCs had spindle-shape during primary culture but acquired characteristic fibroblast-like features when cultured in PL, FFP and UCS. DPSCs in SCM, SFM and PL had significantly (P<0.05) higher proliferative potential than those in UCS and FFP and these observations were supported by PDT values. The CFU efficiency of DPSCs was confirmed in all culture conditions with a slightly varied clonogenic potential in blood-derived components. Based on the growth kinetics and CFU ability, it is concluded that PL could be considered as a suitable alternative to FBS for the ex vivo expansion of DPSCs.

scholarly journals Isolation of Human Dental Pulp Stem Cells and Their Characteristics Before and After Cryopreservation

2021 ◽  
Vol 31 (1) ◽  
pp. 58-69
Author(s):  
Svitlana Mazur ◽  
◽  
Olena Rogulska ◽  
Olena Revenko ◽  
Nataliya Volkova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Third Molar ◽  
Tooth Germ ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Dental Pulp Stem Cells ◽  
Slow Cooling ◽  
Differentiation Potential ◽  
Before And After ◽  
Human Dental Pulp

Dental pulp stem cells (DPSCs) from human third molar tooth germ (wisdom tooth) were isolated using a collagenasebased enzymatic method, the obtained cells were analyzed as for morphology in monolayer culture, immunophenotype, proliferation and differentiation potential before and after cryopreservation. In this study, we showed that based on morphological features, surface markers profile and differentiation potential, the isolated DPSCs corresponded to multipotent mesenchymal stromal cells. DPSCs cryopreservation by slow cooling (1 °С / min) down to –80°C with subsequent immersion into liquid nitrogen in cryoprotectant-free culture medium led to cell death. Cryopreservation using the same protocol in the presence of 10% dimethyl sulfoxide (DMSO) and 20% serum ensured (82 ± 6)% cell viability; while metabolic and proliferative activity, as well as the ability to differentiate into the osteo- and adipogenic lineages of cryopreserved DPSCs were similar to their non-cryopreserved counterparts.

Effect of zoledronate, a third-generation bisphosphonate, on proliferation and apoptosis of human dental pulp stem cells

2018 ◽  
Vol 96 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Solmaz Pourgonabadi ◽  
Seyed Hadi Mousavi ◽  
Zahra Tayarani-Najaran ◽  
Ahmad Ghorbani
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Third Molar ◽  
Osteonecrosis Of The Jaw ◽  
Dental Pulp Stem Cells ◽  
The Body ◽  
Third Molar Extraction ◽  
Thiazolyl Blue Tetrazolium Bromide ◽  
Human Dental Pulp ◽  
Apoptotic Effects

Clinical use of zoledronate is accompanied by osteonecrosis of the jaw but the pathogenesis is not well understood. We assumed that zoledronate may have cytotoxicity against stem cells of the oral cavity and in this way helps to initiate or promote osteonecrosis. Dental pulp stem cells (DPSCs) and gingival fibroblasts (GFs) were isolated from volunteers who were undergoing a third molar extraction. The proliferation of DPSCs and GFs was evaluated using the thiazolyl blue tetrazolium bromide assay. The effect of zoledronate on apoptosis was determined by propidium iodide staining and Western blotting analysis. Incubation with zoledronate for 72 h and 7 days significantly decreased proliferation of DPSCs and GFs at concentrations of more than 0.4 μmol/L (p < 0.001). The IC50 of zoledronate was lower for DPSCs than for GFs (0.92 versus 3.5 μmol/L for 7 days of treatment). After 72 h of treatment with zoledronate, the percentage of apoptotic DPSCs significantly increased, which was accompanied by an increased level of pro-apoptotic proteins caspase-3 and Bax and decreased the level of the anti-apoptotic protein Bcl-2. In conclusion, zoledronate has anti-proliferative and pro-apoptotic effects in DPSCs. These effects may be involved in promoting zoledronate-induced osteonecrosis and suggest an unfavorable impact of this drug on regenerative potentials of the body stem cells.

Characterization of Dental Pulp Stem Cells of Human Tooth Germs

2008 ◽  
Vol 87 (7) ◽  
pp. 676-681 ◽  
Author(s):  
T. Takeda ◽  
Y. Tezuka ◽  
M. Horiuchi ◽  
K. Hosono ◽  
K. Iida ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Tooth ◽  
Dna Array ◽  
Human Dental Pulp ◽  
Tooth Germs

In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. In this present study, we characterized hDPSCs isolated from an earlier developmental stage to evaluate the potential usage of these cells for tissue-regenerative therapy. hDPSCs isolated at the crown-completed stage showed a higher proliferation rate than those isolated at a later stage. When the cells from either group were cultured in medium promoting differentiation toward cells of the osteo/odontoblastic lineage, both became alkaline-phosphatase-positive, produced calcified matrix, and were also capable of forming dentin-like matrix on scaffolds in vivo. However, during long-term passage, these cells underwent a change in morphology and lost their differentiation ability. The results of a DNA array experiment showed that the expression of several genes, such as WNT16, was markedly changed with an increasing number of passages, which might have caused the loss of their characteristics as hDPSCs.

Functional and molecular characterization of transmembrane intracellular pH regulators in human dental pulp stem cells

2018 ◽  
Vol 90 ◽  
pp. 19-26 ◽  
Author(s):  
Gunng-Shinng Chen ◽  
Shiao-Pieng Lee ◽  
Shu-Fu Huang ◽  
Shih-Chi Chao ◽  
Chung-Yi Chang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Molecular Characterization ◽  
Intracellular Ph ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Characterization of SSEA-3 Positive Cells Derived from Human Dental Pulp Stem Cells

2019 ◽  
Vol 28 (4) ◽  
pp. 335-340
Author(s):  
Takatoshi Nagano ◽  
Taichiro Funatsu ◽  
Kazuhiro Gomi
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp
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