Effect of zoledronate, a third-generation bisphosphonate, on proliferation and apoptosis of human dental pulp stem cells

2018 ◽  
Vol 96 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Solmaz Pourgonabadi ◽  
Seyed Hadi Mousavi ◽  
Zahra Tayarani-Najaran ◽  
Ahmad Ghorbani
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Third Molar ◽  
Osteonecrosis Of The Jaw ◽  
Dental Pulp Stem Cells ◽  
The Body ◽  
Third Molar Extraction ◽  
Thiazolyl Blue Tetrazolium Bromide ◽  
Human Dental Pulp ◽  
Apoptotic Effects

Clinical use of zoledronate is accompanied by osteonecrosis of the jaw but the pathogenesis is not well understood. We assumed that zoledronate may have cytotoxicity against stem cells of the oral cavity and in this way helps to initiate or promote osteonecrosis. Dental pulp stem cells (DPSCs) and gingival fibroblasts (GFs) were isolated from volunteers who were undergoing a third molar extraction. The proliferation of DPSCs and GFs was evaluated using the thiazolyl blue tetrazolium bromide assay. The effect of zoledronate on apoptosis was determined by propidium iodide staining and Western blotting analysis. Incubation with zoledronate for 72 h and 7 days significantly decreased proliferation of DPSCs and GFs at concentrations of more than 0.4 μmol/L (p < 0.001). The IC50 of zoledronate was lower for DPSCs than for GFs (0.92 versus 3.5 μmol/L for 7 days of treatment). After 72 h of treatment with zoledronate, the percentage of apoptotic DPSCs significantly increased, which was accompanied by an increased level of pro-apoptotic proteins caspase-3 and Bax and decreased the level of the anti-apoptotic protein Bcl-2. In conclusion, zoledronate has anti-proliferative and pro-apoptotic effects in DPSCs. These effects may be involved in promoting zoledronate-induced osteonecrosis and suggest an unfavorable impact of this drug on regenerative potentials of the body stem cells.

scholarly journals Isolation of Human Dental Pulp Stem Cells and Their Characteristics Before and After Cryopreservation

2021 ◽  
Vol 31 (1) ◽  
pp. 58-69
Author(s):  
Svitlana Mazur ◽  
◽  
Olena Rogulska ◽  
Olena Revenko ◽  
Nataliya Volkova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Third Molar ◽  
Tooth Germ ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Dental Pulp Stem Cells ◽  
Slow Cooling ◽  
Differentiation Potential ◽  
Before And After ◽  
Human Dental Pulp

Dental pulp stem cells (DPSCs) from human third molar tooth germ (wisdom tooth) were isolated using a collagenasebased enzymatic method, the obtained cells were analyzed as for morphology in monolayer culture, immunophenotype, proliferation and differentiation potential before and after cryopreservation. In this study, we showed that based on morphological features, surface markers profile and differentiation potential, the isolated DPSCs corresponded to multipotent mesenchymal stromal cells. DPSCs cryopreservation by slow cooling (1 °С / min) down to –80°C with subsequent immersion into liquid nitrogen in cryoprotectant-free culture medium led to cell death. Cryopreservation using the same protocol in the presence of 10% dimethyl sulfoxide (DMSO) and 20% serum ensured (82 ± 6)% cell viability; while metabolic and proliferative activity, as well as the ability to differentiate into the osteo- and adipogenic lineages of cryopreserved DPSCs were similar to their non-cryopreserved counterparts.

scholarly journals The Effect of Three Different Biomaterials on Proliferation and Viability of Human Dental Pulp Stem Cells (In-vitro Study)

2017 ◽  
Vol 5 (5) ◽  
pp. 657-663 ◽  
Author(s):  
Dalia A. Mohamed ◽  
Maha I. Abdelfattah ◽  
Eman H. A. Aboulezz
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Critical Role ◽  
Third Molar ◽  
Dental Pulp Stem Cells ◽  
Hydroxy Apatite ◽  
Cell Counts ◽  
Human Dental Pulp ◽  
Viability Percentage

BACKGROUND: Biomaterial cytotoxicity on dental stem cells plays a critical role in managing the regeneration of dental tissue.AIM: The aim of the present study was to evaluate the effect of Nano-hydroxy apatite (NHA), Mineral trioxide aggregate (MTA), and Calcium-enriched mixture (CEM) on the proliferation, and viability of human dental pulp stem cells (hDPSCs) isolated from third molar teeth.METHODS: Cultured DPSCs were characterized and the tested biomaterials were shaped into cylinders then inserted directly on the DPSCs. Proliferation and viability percentage of DPSCs were evaluated at 1, 3, 5, 7, 9, 11, and 14 days of culture.RESULTS: The biomaterials supplemented DPSCs showed a significant initial decrease in cell count and viability percentage at day one. Then, a rise in cell counts and viabilities was noticed after that. There was a decrease in cell counts, and viabilities in the NHA supplemented cells in comparison to other tested biomaterials.CONCLUSIONS: All tested biomaterials maintain the proliferation of DPSCs for different durations. NHA showed less proliferative and more cytotoxic effect than other tested materials.

Chrysin induces osteogenic differentiation of human dental pulp stem cells

2021 ◽  
Vol 400 (2) ◽  
pp. 112466
Author(s):  
J.F. Huo ◽  
M.L. Zhang ◽  
X.X. Wang ◽  
D.H. Zou
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Methylglyoxal-Dependent Glycative Stress Is Prevented by the Natural Antioxidant Oleuropein in Human Dental Pulp Stem Cells through Nrf2/Glo1 Pathway

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 716
Author(s):  
Simona Delle Delle Monache ◽  
Fanny Pulcini ◽  
Roberta Frosini ◽  
Vincenzo Mattei ◽  
Vincenzo Nicola Talesa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Great Promise ◽  
Oral Diseases ◽  
Bioactive Component ◽  
Glycation End Products ◽  
Abnormal Accumulation ◽  
Synthetic Agents ◽  
Human Dental Pulp

Methylglyoxal (MG) is a potent precursor of glycative stress (abnormal accumulation of advanced glycation end products, AGEs), a relevant condition underpinning the etiology of several diseases, including those of the oral cave. At present, synthetic agents able to trap MG are known; however, they have never been approved for clinical use because of their severe side effects. Hence, the search of bioactive natural scavengers remains a sector of strong research interest. Here, we investigated whether and how oleuropein (OP), the major bioactive component of olive leaf, was able to prevent MG-dependent glycative stress in human dental pulp stem cells (DPSCs). The cells were exposed to OP at 50 µM for 24 h prior to the administration of MG at 300 µM for additional 24 h. We found that OP prevented MG-induced glycative stress and DPSCs impairment by restoring the activity of Glyoxalase 1 (Glo1), the major detoxifying enzyme of MG, in a mechanism involving the redox-sensitive transcription factor Nrf2. Our results suggest that OP holds great promise for the development of preventive strategies for MG-derived AGEs-associated oral diseases and open new paths in research concerning additional studies on the protective potential of this secoiridoid.

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